期刊论文

Ma, H.X.

中文

中药材

1001-4454

2008

31

8

1201-1204

10.13863/j.issn1001-4454.2008.08.039

广东省自然科学基金（5300997） 广州市卫生局资助项目（2005A041）

本校为其他机构

目的：探讨菟丝子总黄酮对大鼠流产模型蜕膜PR及Th1／Th2细胞因子表达的影响，同时观察血清P和PR的变化。方法：对溴隐亭致sD孕鼠流产模型，设菟丝子总黄酮高、低剂量治疗组、达芙通为阳性对照组，于孕12d处死各组大鼠，酶联免疫检测血清P和PRL的水平，免疫组化法检测蜕膜PR的表达，RT-PCR法检测蜕膜Th1／Th2mRNA的表达。结果：模型组蜕膜IL4、IL-10mRNA及PR蛋白表达明显低于正常妊娠组，TNF-α、IFN-γ,／mRNA表达明显高于正常妊娠组，差异有显著性（P〈0．05），菟丝子总黄酮可上调流产大鼠蜕膜IL4、IL-10、PR的表达，下调TNF-α、IFN-γ,／表达，同时可提高血清P的水平，其作用与孕激素达芙通...

To study the effect of TFCC on expression of Th type-1/Th type-2 cytokines and PR in rats abortion model, and observe the changes of serum P and PRL. Early abortion rat model was established by Bromocriptine at 6-8 gestation day, the model rats were treated with high and low dosage of TFCC and the progesterone as the positive control. Progesterone and prolactin levels in the serum were measured by radioimmunoassay, the protein expression of PR was inspected by immunohistochemical, RT-PCR was applied to determine the expression of Thl/Th2 mRNA on deciduas. In the deciduas of model group, IL-4 a...