期刊论文

Effects of different formulae composed from the therapeutic principle of Qiboosting toxin-resolving on migrating potentiality and associated gene expressive activities of CNE2Z NPC cells

中文

中国中西医结合耳鼻咽喉科杂志

1007-4856

2013

3

165-170,198

10.3969/j.issn.1007-4856.2013.03.002

81072904:国家自然科学基金 30973856:国家自然科学基金 2009FJ3202:湖南省科技厅项目 2012FJ6124:湖南省科技厅项目 06WK3016:湖南省科技厅国际科技合作项目 12C1257:教育厅项目 :湖南省海外名师项目

本校为其他机构

　　目的观察益气解毒法不同组方对CNE2Z鼻咽癌细胞迁徙运动潜能的影响并探讨其效应与相关基因表达活性变化的关系。方法依据益气解毒法组成原方并在此基础上依法构建改方，分别制备不同浓度含药血浆并作用于相同靶细胞CNE2Z鼻咽癌细胞，划痕法检测药物干预前后靶细胞的迁徙运动潜能，免疫组化法和RT-PCR法分别检测干预前后靶细胞nm23和c-myc蛋白表达水平和mRNA转录活性，比较分析组间差异。结果细胞迁徙运动能力检测结果显示，原方呈现抑制效应而改方表现则促进效应(P＜0.05)。免疫组化检测结果，在c-myc与nm23-H1蛋白表达活性上，原方对前者表达活性无明显影响但后者显著增强，差异均有统计学...

Objective To investigate the effect of different formulae composed from the therapeutic principle of Qi-boosting toxin-resolving on the migrating potentiality and associated gene expressive activities of nasopharyngeal carcinoma (NPC) cell line CNE2Z cells. Methods Firstly, different formulae derived from the same therapeutic principle of Qi-boosting toxin-resolving were composed only with their dosages changed but not with their composing kinds of herbs, i.e. the original formula (OF) and the modified one (MF). Herbal medicines-containing plasma (HMCP) were prepared routinely from rats by fee...