Objective To construct an alpha enolase (ENO1) recombinant expression plasmid, and study its expression form. Methods The cDNA was synthesized using total RNA of Human Umbilical Vein Endothelial Cel (HUVEC) as the template. ENO1 gene was amplified from cDNA using specific forward and reverse primers by polymerase chain reaction (PCR). The recombinant expression vector was obtained by ligating ENO1 gene and pET28 (a) using T4 ligase. ENO1 protein was produced by transfecting expression vector pET28 (a)-ENO1 into BL21 (DE3) E.Coli. and subsequent IPTG induction. The supernatant and bacteria prec...
