期刊论文

中文

中华中医药杂志

1673-1727

2013

28

7

2135-2137

国家自然科学基金（No.30801496） 湖南省教育厅科研基金（No.09C722）.

本校为第一机构

目的:探讨桔梗总皂苷对人肺腺癌A549细胞周期及增殖抑制作用的影响。方法:体外培养肺腺癌细胞系A549细胞,MTT法检测桔梗总皂苷对人肺腺癌A549细胞增殖的影响;Giemsa染色法观察细胞形态学变化;流式细胞术检测桔梗总皂苷对A549细胞周期的影响。结果:MTT法检测显示,桔梗总皂苷能明显抑制人肺腺癌A549细胞的增殖,其抑制作用随药物浓度的增加而增强,呈剂量依赖关系(P

Objective： To investigate the inhibiting effects of total platycodin on cell cycle and proliferation of human pulmonary adenocarcinoma A549 ceils. Methods： The pulmonary adenocarcinoma A549 cells were cultred in vitro. The proliferation, variation of cytomorphology and total platycodin were detected by MTT, Giemsa staining and FCM （flow cytometry） methods respectively. Results： The results showed that cell proliferation was inhibited apparently by total platycodin with dose-effect relationship （P〈0.05）. The cytomorphology of A549 cells ...