期刊论文

Peng, Qing-Hua

英文

国际眼科杂志

1672-5123

2010

3

1

5-8

10.3969/j.issn.1672-5123.2010.01.002

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30772824]; Natural Science Foundation of Hunan Province of ChinaNatural Science Foundation of Hunan Province [07JJ3049]; Doctor-Subject Site Special-Purpose Foundation of High-School of China [200805410004]; Scientific Research Foundation of Hunan Provincial Science and Technology Department of China [2009FJ3001]; Graduation Innovate Foundation of Hunan Province in 2008 of China

本校为通讯机构

• AIM: To explore the effect of Buddleia flavonoids drugcontaining plasma and androgen receptor(AR) blocker on the expression of STAT1 phosphoprotein. • METHODS: In vitro lacrimal gland epithelial cells were cultivated with H2 O2 to establish the dry eye apoptosis state. Blank plasma group, Buddleia officinalis plasma total flavonoids interfere with drug-containing group, and the intervention group of testosterone propionate were set. The expressions of STAT1 phosphoprotein of each group were observed by Western blot and AR blocker flutamide ...

目的:探讨密蒙花总黄酮含药血浆和雄激素受体阻滞剂对泪腺上皮细胞中STAT1的磷酸化蛋白表达的影响.方法:体外分离及培养泪腺上皮细胞.以H2O2诱导大鼠泪腺上皮细胞凋亡,建立雄激素水平下降所致干眼症的细胞凋亡状态.设立空白血浆组、密蒙花总黄酮含药血浆干预组、丙酸睾酮干预组,分别观察各组STAT1的磷酸化蛋白表达情况;并应用雄激素受体阻滞剂氟他胺,考察密蒙花总黄酮的拟雄激素效应.结果:免疫印迹结果表明,含药血浆干预后,密蒙花总黄酮含药血浆干预组中(0.353±0.494)与丙酸睾酮干预组中STAT1的磷酸化蛋白(0.502±0.036)的表达增强,两组间的差异有统计学意义(P