期刊论文

Effects and mechanisms of nicotinic acid curcumin on apoptosis induced by low shear stress in human umbilical vein endothelial cells

中文

中国临床药理学与治疗学

1009-2501

2018

23

8

855-861

10.12092/j.issn.1009-2501.2018.08.003

国家自然科学基金（81673722） 湖南省教育厅重点项目（201515A） 湖南省自然科学基金资助项目（2015JJ4097） 湖南省大学生研究性学习和创新性实验计划项目（2017286）

本校为第一机构

目的:探讨烟酸姜黄素酯(curcumin nicotinate,CN)对低剪切应力(low shear stress,LSS)诱导人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)凋亡的影响及机制研究。方法:运用平行平板流动腔建立LSS诱导的HUVECs凋亡模型。采用Hoechst33258荧光染色,流式细胞术检测CN对LSS诱导的HUVECs凋亡的影响。Western blot检测CN对LSS诱导的HUVECs中前蛋白转化酶枯草溶菌素9 (proprotein convertase subtilisin /kexin type 9,PCSK9)蛋白表达的影响。结果: Hoechst33258荧光染色结果显示,与静态培养组比较,LSS组(3 dyne /cm~2,12 h)内呈现凋亡状态的HUVECs明显增多。与LSS组比较,各CN + LS...

AIM: To investigate the effect of curcumin nicotinate(CN) on apoptosis of human umbilical vein endothelial cells(HUVECs) induced by low shear stress(LSS) and its mechanism.METHODS: A parallel plate flow chamber was used to establish a model of HUVECs apoptosis induced by low shear stress.Hoechst33258 fluorescence staining and flow cytometry were used to detect the effects of curcumin on the apoptosis of HUVECs.Western blot was used to detect the expression of proprotein convertase subtilisin /kexin type 9.RESULTS: Hoechst 33258 fluorescence sta...