期刊论文

Ma, H.X.

中文

中药材

1001-4454

2009

32

6

939-943

10.3321/j.issn:1001-4454.2009.06.036

基金项目：广东省自然科学基金（5300997） 广州市卫生局项目（2005A041）

本校为其他机构

目的:探讨菟丝子总黄酮对早孕期人细胞滋养细胞增殖的影响及其信号转导机制.方法:应用硅化聚乙酞胺吡咯烷酮(Percoll)密度梯度离心法,分离纯化早孕期人细胞滋养细胞,经免疫细胞化学法鉴定其分子表达特征和纯度;采用MTT法评价早孕期人细胞滋养细胞活力;流式细胞术分析细胞滋养细胞PCNA的表达;用Western blotting法观察Perk1/2的水平.结果:菟丝子总黄酮呈时间和剂量依赖性增强早孕期人细胞滋养细胞活力,促进细胞滋养细胞ERK1/2的磷酸化.结论:菟丝子总黄酮可能通过活化MAPK/ERK1/2信号通路促进人细胞滋养细胞增殖.

Objective ： To investigate the effect of total flavones from Cuscuta chinens is （TFCC） on proliferation of the first trimes- ter human cytotrophoblast and its possible signal transduction pathway. Methods： The first trimester human trophoblast cells were isola- ted by trypsin/Dnasel digestion and discontinuous Percoll density gradient centrifugation. After identified by immunocytochemistry to Cytokeratin 7 and Vimentin, the trophoblast cells appared to more than 95% pure. MTT Assay was used to evaluate the effect of TFCC in vitro viability/...