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MicroRNA-375 promotes 3T3-L1 adipocyte differentiation through modulation of extracellular signal-regulated kinase signalling.

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WOS被引频次:55
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成果类型:
期刊论文
作者:
Ling, Hong-Yan;Wen, Ge-Bo;Feng, Shui-Dong;Tuo, Qin-Hui;Ou, He-Sheng;Yao, Chao Hua;Zhu, Bing-Yang;Gao, Zhi-Ping;Zhang, Liang;Liao, Duan-Fang
通讯作者:
Zhang, LA
作者机构:
[Liao, Duan-Fang; Tuo, Qin-Hui; Ou, He-Sheng; Zhu, Bing-Yang; Gao, Zhi-Ping] Univ S China, Inst Pharm & Pharmacol, Key Lab Pharmacoprote Hunan Prov, Hengyang, Peoples R China.
[Liao, Duan-Fang] Hunan Univ Chinese Med, Sch Pharm, Dept Tradit Chinese Diagnost, Changsha, Hunan, Peoples R China.
[Ling, Hong-Yan] Univ S China, Sch Med, Dept Physiol, Hengyang, Peoples R China.
[Ling, Hong-Yan; Wen, Ge-Bo] Univ S China, Affiliated Hosp 1, Inst Clin Res, Hengyang, Peoples R China.
[Zhang, Liang] Palmer Coll Chiropract Florida, Palmer Ctr Chiropract Res, Lab Cell & Mol Biol, Port Orange, FL 32129 USA.
通讯机构:
[Zhang, LA] Palmer Coll Chiropract Florida, Palmer Ctr Chiropract Res, Lab Cell & Mol Biol, 4705 S Clyde Morris Blvd, Port Orange, FL 32129 USA.
语种:
英文
关键词:
3T3‐L1 adipocytes;differentiation;extracellular signal‐regulated kinases 1/2;microRNA‐375;obesity
期刊:
Clinical and Experimental Pharmacology and Physiology
ISSN:
0305-1870
年:
2011
卷:
38
期:
4
页码:
239-246
文献类别:
WOS:Article
所属学科:
ESI学科类别:药理学&毒理学
入藏号:
WOS:000288803600007;PMID:21291493
基金类别:
National Natural Science Foundation of China [81000328, 30971170]; National Major Basic Research Program of China (973 Program) [2006CB503808]; Natural Science Foundation of Hunan [07C0050]; Key Discipline in Hunan Province; National Institutes of Health [K01AG21999]
机构署名:
本校为其他机构
院系归属:
医学院
中医学院
第一中医临床学院
药学院
摘要:
P>1. Adipocyte hypertrophy and hyperplasia are important processes in the development of obesity. To understand obesity and its associated diseases, it is important to elucidate the molecular mechanisms governing adipogenesis. MicroRNA-375 has been shown to inhibit differentiation of neurites, and participate in the regulation of insulin secretion and blood homeostasis. However, it is unknown whether miR-375 plays a role in adipocyte differentiation. 2. To investigate the role of miR-375 in adipocyte differentiation, we compared the miR-375 expression level between 3T3-L1 pre-adipocytes and adipocytes using miRNA microarray and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) analysis. Furthermore, we evaluated the effects of overexpression or inhibition of miR-375 on 3T3-L1 adipocyte differentiation. 3. In the present study, we found that miR-375 expression was increased after induction of adipogenic differentiation. Overexpression of miR-375 enhanced 3T3-L1 adipocyte differentiation, as evidenced by its ability to increase mRNA levels of both CCAAT/enhancer binding protein-alpha (C/EBP alpha) and peroxisome proliferator-activated receptor-gamma (PPAR gamma 2), and induction of adipocyte fatty acid-binding protein (aP2) and triglyceride (TG) accumulation. Furthermore, we found overexpression of miR-375 suppressed phosphorylation levels of extracellular signal-regulated kinases 1/2 (ERK1/2). In contrast, anti-miR-375 increased ERK1/2 phosphorylation levels and inhibited mRNA expression of C/EBP alpha, PPAR gamma 2 and aP2 in 3T3-L1 adipocyte, accompanied by decreased adipocyte differentiation. 4. Taken together, these data suggest that miR-375 promotes 3T3-L1 adipocyte differentiation, possibly through modulating the ERK-PPAR gamma 2-aP2 pathway.
参考文献:
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Bartel DP, 2004, CELL, V116, P281, DOI 10.1016/S0092-8674(04)00045-5
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