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MicroRNA‐375 promotes 3T3‐L1 adipocyte differentiation through modulation of extracellular signal‐regulated kinase signalling

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WOS被引频次:65
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成果类型:
期刊论文
作者:
Ling, Hong-Yan;Wen, Ge-Bo;Feng, Shui-Dong;Tuo, Qin-Hui;Ou, He-Sheng;Yao, Chao Hua;Zhu, Bing-Yang;Gao, Zhi-Ping;Zhang, Liang*;Liao, Duan-Fang
通讯作者:
Zhang, Liang
作者机构:
[Feng, Shui-Dong] Univ S China, Sch Publ Hlth, Dept Epidemiol, Hengyang, Peoples R China.
[Gao, Zhi-Ping; Zhu, Bing-Yang; Ou, He-Sheng; Tuo, Qin-Hui; Liao, Duan-Fang] Univ S China, Inst Pharm & Pharmacol, Key Lab Pharmacoprote Hunan Prov, Hengyang, Peoples R China.
[Wen, Ge-Bo; Ling, Hong-Yan] Univ S China, Affiliated Hosp 1, Inst Clin Res, Hengyang, Peoples R China.
[Zhang, Liang] Palmer Coll Chiropract Florida, Palmer Ctr Chiropract Res, Lab Cell & Mol Biol, Port Orange, FL 32129 USA.
[Zhang, Liang] Palmer Coll Chiropract Florida, Palmer Ctr Chiropract Res, Lab Cell & Mol Biol, 4705 S Clyde Morris Blvd, Port Orange, FL 32129 USA.
通讯机构:
[Zhang, Liang] Palmer Coll Chiropract Florida, Palmer Ctr Chiropract Res, Lab Cell & Mol Biol, 4705 S Clyde Morris Blvd, Port Orange, FL 32129 USA.
语种:
英文
关键词:
3T3‐L1 adipocytes;differentiation;extracellular signal‐regulated kinases 1/2;microRNA‐375;obesity
期刊:
Clinical and Experimental Pharmacology and Physiology
ISSN:
1440-1681
年:
2011
卷:
38
期:
4
页码:
239-246
文献类别:
WOS:Article
所属学科:
ESI学科类别:药理学&毒理学;WOS学科类别:Pharmacology & Pharmacy;Physiology
入藏号:
WOS:000288803600007;PMID:21291493
基金类别:
National Natural Science Foundation of China [81000328, 30971170]; National Major Basic Research Program of China (973 Program) [2006CB503808]; Natural Science Foundation of Hunan [07C0050]; Key Discipline in Hunan Province; National Institutes of Health [K01AG21999]
机构署名:
本校为其他机构
院系归属:
药学院
摘要:
1. Adipocyte hypertrophy and hyperplasia are important processes in the development of obesity. To understand obesity and its associated diseases, it is important to elucidate the molecular mechanisms governing adipogenesis. MicroRNA‐375 has been shown to inhibit differentiation of neurites, and participate in the regulation of insulin secretion and blood homeostasis. However, it is unknown whether miR‐375 plays a role in adipocyte differentiation. 2. To investigate the role of miR‐375 in adipocyte differentiation, we compared the miR‐375 expression level between 3T3‐L1 pre‐adipocytes and adipocytes using miRNA microarray and quantitative real‐time reverse transcription polymerase chain reaction (qRT‐PCR) analysis. Furthermore, we evaluated the effects of overexpression or inhibition of miR‐375 on 3T3‐L1 adipocyte differentiation. 3. In the present study, we found that miR‐375 expression was increased after induction of adipogenic differentiation. Overexpression of miR‐375 enhanced 3T3‐L1 adipocyte differentiation, as evidenced by its ability to increase mRNA levels of both CCAAT/enhancer binding protein‐α (C/EBPα) and peroxisome proliferator‐activated receptor‐γ (PPARγ2), and induction of adipocyte fatty acid‐binding protein (aP2) and triglyceride (TG) accumulation. Furthermore, we found overexpression of miR‐375 suppressed phosphorylation levels of extracellular signal‐regulated kinases 1/2 (ERK1/2). In contrast, anti‐miR‐375 increased ERK1/2 phosphorylation levels and inhibited mRNA expression of C/EBPα, PPARγ2 and aP2 in 3T3‐L1 adipocyte, accompanied by decreased adipocyte differentiation. 4. Taken together, these data suggest that miR‐375 promotes 3T3‐L1 adipocyte differentiation, possibly through modulating the ERK–PPARγ2–aP2 pathway.
参考文献:
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Bartel DP, 2004, CELL, V116, P281, DOI 10.1016/S0092-8674(04)00045-5
Bhattacharya I, 2006, FEBS LETT, V580, P5765, DOI 10.1016/j.febslet.2006.09.032
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