期刊论文

Effects of Zuogui Jiangtang Jieyu Prescription on Hippocampal Neurons in Simulated Diabetes Mellitus with Depression

中文

中国中医药信息杂志

1005-5304

2019

26

1

51-56

10.3969/j.issn.1005-5304.2019.01.013

国家科技重大专项(2017ZX09309026) 国家自然科学基金(81573965) 湖南省教育厅优秀青年资助项目(17B201).

本校为第一机构

目的 观察左归降糖解郁方对模拟糖尿病并发抑郁症(DD)环境下海马神经元的影响,探讨其可能的作用机制.方法 海马神经元原代细胞取自受孕18 d SD大鼠胎鼠,葡萄糖联合皮质酮干预构建DD海马神经元细胞模型.将培养的海马神经元随机分为正常组、空白血清组、模型组、阳性药(二甲双胍十氟西汀)药物血清组、中药(左归降糖解郁方)药物血清组和阻断剂组.正常组和模型组给予等量培养液,阻断剂组给予促肾上腺皮质激素释放激素1型受体(CRHR1)阻断剂安塔拉明200 μmol/L,其余组给予体积分数为10％的药物血清或空白血清,造模干预18h后,尼氏染色检测海马神经元损伤情况,高内涵细胞成像分析(HCA)技术检测CRHR1、突...

Objective To explore the effects of Zuogui Jiangtang Jieyu Prescription on hippocampal neurons in simulated diabetes mellitus with depression (DD); To discuss its possible mechanism. Methods Hippocampal neurons from fetal rats of 18 d pregnant rats were primitively cultivated, and the glucose combined with corticosterone was used to establish DD hippocampal neuronal cell model. Cultured hippocampal neurons were randomly divided into normal group, blank serum group, model group, positive drug (metformin plus fluoxetine) serum group, TCM (Zuogui ...