期刊论文

中文

癌变·畸变·突变

1004-616X

2008

20

5

363-366

10.3969/j.issn.1004-616X.2008.05.007

30672738,30572408:国家自然科学基金 03JJY1006:湖南省***基金 06WK3016:湖南省科技厅国际科技合作项目 06B070:湖南省教育厅优秀青年项目

本校为第一机构

背景与目的:探讨TgN(p53mt-LMPl) HT小鼠鼻腔和鼻咽黏膜上皮癌前病变发生与细胞周期分布,凋亡相关基因bax、bd-2和增殖相关基因PCNA的表达水平及它们与p53mt基因表达的相关关系.材料与方法:HE染色法观察G代12月龄TgN(p53mt-LMP1) HT转基因阳性和阴性小鼠鼻腔和鼻咽黏膜上皮病理组织学变化,流式细胞术测定细胞周期分布特点,免疫组织化学法检测组织中p53mt、bax、bcl-2和PCNA表达水平,综合分析其相关性.结果:转基因阴性小鼠和阳性小鼠鼻腔或鼻咽黏膜上皮癌前病变发生率分别为0和63.64%(P

BACKGROUND AND AIM: To investigate the association of precancerous lesion development with cell cycle characteristics and expressive activities of genes such as bax,bcl-2 and PCNA and the relationship between these and p53mt expression in nasal and nasopharyngeal epithelia of TgN(p53mt-LMP1)/HT mice containing human mutant p53(p53mt) gene and EB virus latent membrane protein 1(LMP1).MATERIALS AND METHODS: The pathohistological changes in nasal and nasopharyngeal epithelial tissues in the fourth generation transgenic positive mice and negative mice aged 12 months were determined by H-E staining...