期刊：
Journal of Ginseng Research,2024年 ISSN：1226-8453
通讯作者：
Hongxin Wang
作者机构：
[Chenyang Ran; Meili Lu; Yi Hao; Xinyu Guo; Yunhan Li; Hongxin Wang] The Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Jinzhou Medical University, Jinzhou, 121001, PR China;[Fang Zhao] Institute of Innovation and Applied Research in Chinese Medicine and Department of Rheumatology of the First Hospital, Hunan University of Chinese Medicine. Changsha, Hunan, 410208, PR China;[Yuhong Su] The College of Food and Health of Liaoning Province, Jinzhou Medical University, Jinzhou, PR China
通讯机构：
[Hongxin Wang] T;The Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Jinzhou Medical University, Jinzhou, 121001, PR China
摘要：
Hypoxic pulmonary hypertension (HPH) is the main pathological change in vascular remodeling, a complex cardiopulmonary disease caused by hypoxia. Some research results have shown that ginsenoside Rg1 (Rg1) can improve vascular remodeling, but the effect and mechanism of Rg1 on hypoxia-induced pulmonary hypertension are not clear. The purpose of this study was to discuss the potential mechanism of action of Rg1 on HPH.
C57BL/6 mice, calpain-1 knockout mice and Pulmonary artery smooth muscle cells (PASMCs) were exposed to a low oxygen environment with or without different treatments. The effect of Rg1 and calpain-1 silencing on inflammation, fibrosis, proliferation and the protein expression levels of calpain-1, STAT3 and p-STAT3 were determined at the animal and cellular levels.
At the mouse and cellular levels, hypoxia promotes inflammation, fibrosis, and cell proliferation, and the expression of calpain-1 and p-STAT3 is also increased. Ginsenoside Rg1 administration and calpain-1 knockdown, MDL-28170, and HY-13818 treatment showed protective effects on hypoxia-induced inflammation, fibrosis, and cell proliferation, which may be associated with the downregulation of calpain-1 and p-STAT3 expression in mice and cells. In addition, overexpression of calpain 1 increased p-STAT3 expression, accelerating the onset of inflammation, fibrosis and cell proliferation in hypoxic PASMCs.
Ginsenoside Rg1 may ameliorate hypoxia-induced pulmonary vascular remodeling by suppressing the calpain-1/STAT3 signaling pathway.
期刊：
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING,2024年 ISSN：1386-2073
作者机构：
[Li, Qing; Li, Xiao Ling; Hou, Chao Wen; Shi, Yuhe; Zhu, Jue; Tong, Qiao Zhen; Liu, Xiang Dan] Department of Hunan University of Chinese Medicine, School of Pharmacy, Changsha 410208, Hunan Province, P.R.China
摘要：
AIMS AND OBJECTIVE: This study aimed to identify the bioactive compounds and explore the multi-target mechanisms of Salvia miltiorrhiza Bunge (SMB) against coronary heart disease (CHD) using an integrated serum pharmacochemistry and network pharmacology approach. MATERIALS AND METHODS: The chemical constituents of SMB were characterized by UPLC-MS. The absorbed ingredients and metabolites after oral SMB administration were identified in rat serum. Therapeutic targets of SMB against CHD were predicted by intersecting the targets of absorbed compounds from databases and CHD-associated genes. Protein-protein interaction network, pathway analysis, molecular docking, and molecular dynamic simulation were performed. RESULTS: A total of 61 SMB-derived compounds were identified in rat serum. Network analysis revealed 111 candidate targets highly related to CHD pathways. Further topological analysis identified 10 hub targets and 20 key active compounds, constructing an informative compoundtarget- pathway network. PTGS2 and TNF were predicted as primary targets of SMB against CHD based on molecular dynamic simulation. CONCLUSION: This integrated approach identified bioactive compounds and multi-target mechanisms of SMB against CHD. The results provide scientific evidence supporting SMB's clinical efficacy and reveal potential anti-CHD targets.
摘要：
Seco-triterpenoids are a unique class of triterpenoids possessing distinct structural features. Based on the differences in their parent nucleus, they can be divided into two categories: tetracyclic triterpenes and pentacyclic triterpenes, which are widely distributed across various plant species. Previous studies indicate that natural seco-triterpenoids have diverse chemical structures and exhibit extensive biological activities, including anti-tumor, anti-inflammatory, hypoglycemic, and neuroprotective effects. This review comprehensively summarizes recent research (2020–2023) on seco-triterpenoids and derived saponins, encompassing their chemical structures, plant distributions, pharmacological activities, structure–activity relationships, and future development trends. Furthermore, we utilized the latest bibliometric tool, VOSviewer, to conduct a keyword cluster analysis, enabling us to identify current research hotspots and patterns. Besides, we have cataloged and analyzed 351 compounds, inclusive of 298 tetracyclic triterpenoids and 53 pentacyclic triterpenoids, in order to provide valuable references for activity mining and structural modification of seco-triterpenoids, facilitating further clinical applications and developments. Collectively, seco-triterpenoids represent an important class of natural products with significant potential for pharmacological use. By summarizing recent research achievements, this review provides a beneficial resource for scientists in the field, promoting the exploration and development of seco-triterpenoid-based drugs.
期刊：
BALKAN MEDICAL JOURNAL,2024年41(2):130-138 ISSN：2146-3123
作者机构：
[Yang, Renyi; Chen, Hongyao; Li, Kexiong; Zeng, Puhua; Yu, Xiaopeng; Peng, Wei] Hunan Provincial Hospital of Integrated Traditional Chinese and Western, Cancer Research Institute of Hunan Academy of Traditional Chinese Medicine, Hunan Academy of Chinese Medicine, Hunan, China;[Yang, Renyi; Chen, Hongyao; Xue, Peisen] Hunan University of Chinese Medicine, Hunan, China
摘要：
BACKGROUND: The changes in risk scores of inflammatory markers among patients diagnosed with hepatocellular carcinoma (HCC) remain unknown. AIMS: To investigate the relationship between the inflammation risk score and other contributing factors and the prognostic outcomes in patients with moderate and advanced hepatitis B virus (HBV)-related HCC. STUDY DESIGN: A retrospective cohort study. METHODS: A total of 174 patients with moderate and advanced HBV related HCC were recruited to investigate the impact of stratified inflammatory risk scores and other associated risk factors on disease prognosis. Based on the optimal cut-off values calculated by the Youden index, the patients were divided into high-risk and low-risk groups based on their inflammation risk scores. RESULTS: The study found a significant difference in median survival time between the low-risk and high-risk groups based on the inflammation risk score. Furthermore, the inflammation risk score, alpha-fetoprotein levels, transarterial chemoembolization treatment, and Barcelona Clinic Liver Cancer stage were identified as independent prognostic factors. The four variables were used to construct a prognostic nomogram for HCC. Subsequent evaluations using time-dependent receiver operating characteristic analysis and calibration curve tests revealed the nomogram's commendable discriminatory ability. As a result, the nomogram proved to be an effective tool for predicting survival at 2- to 4-years. CONCLUSION: The inflammation risk score has been identified as a significant prognostic factor for HBV-related HCC. The development of nomogram models has provided a practical and effective tool for determining the prognosis of patients affected by HBV-related HCC.
