作者:
Tang, Jiaqi;Gao, Hongyan;Xu, Yanqiu;Chen, Jingru;Wu, Bin
期刊:
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH,2023年15(4):2291-2303 ISSN:1943-8141
通讯作者:
Wu, B
作者机构:
[Tang, Jiaqi; Xu, Yanqiu; Wu, Bin; Wu, B] Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing 400016, Peoples R China.;[Tang, Jiaqi; Xu, Yanqiu; Wu, Bin; Wu, B] Chongqing Hosp Tradit Chinese Med, Dept Rheumatol, Chongqing 400021, Peoples R China.;[Gao, Hongyan] Chongqing Key Lab Tradit Chinese Med Prevent & Tre, R China, Chongqing 400021, Peoples R China.;[Chen, Jingru] Hunan Univ Tradit Chinese Med, Changsha 410208, Peoples R China.;[Wu, Bin; Wu, B] Chongqing Hosp Tradit Chinese Med, Dept Rheumatol, 6 Pan Xi Qi Zhi Rd, Chongqing 400021, Peoples R China.
通讯机构:
[Wu, B ] C;Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing 400016, Peoples R China.;Chongqing Hosp Tradit Chinese Med, Dept Rheumatol, Chongqing 400021, Peoples R China.;Chongqing Hosp Tradit Chinese Med, Dept Rheumatol, 6 Pan Xi Qi Zhi Rd, Chongqing 400021, Peoples R China.
摘要:
The annual incidence of gout is increasing along with lifestyle and dietary changes. Accumulation of urate crystals in joints and tissues when the amount of uric acid exceeds its saturation concentration causes acute inflammation that leads to gout. Reducing the serum uric acid concentration is the key to treating gout. Allopurinol, febuxostat, benzbromarone, and other drugs are effective, but side effects of treatment such as toxicity and recurrence after drug withdrawal cannot be ignored. Recent studies have found that many Chinese medicines are effective and safe, provide durable efficacy, and are associated with low recurrence rates. This article reviews recent investigations of Chinese medicines for lowering uric acid, including components such as berberine, luteolin, and others; single medicines such as Smilax glabra Roxb., Reynoutria japonica Houtt., and Plantago asiatica L.; and compounds such as Wuling Powder and Compound Tufuling Granules. Mechanisms of lowering uric acid, including inhibiting uric acid production and promoting uric acid excretion are discussed. Clinical studies and basic research are reviewed.
期刊:
Mediators of Inflammation,2023年2023 ISSN:0962-9351
通讯作者:
Jian-Hu Fan<&wdkj&>Guo-Huang Hu<&wdkj&>Da-Hua Wu<&wdkj&>Li Mao
作者机构:
[Mao, Li] Changsha Hlth Vocat Coll, Dept Basic Med, Changsha 410600, Hunan, Peoples R China.;[Fan, Jian-Hu; Wu, Da-Hua] Hunan Acad Tradit Chinese Med, Affiliated Hosp, Dept Neurol, Changsha 410006, Hunan, Peoples R China.;[Hu, Guo-Huang] Hunan Normal Univ, Affiliated Changsha Hosp, Changsha 410006, Hunan, Peoples R China.
关键词:
Introduction;Materials and Methods;Results;Discussion;Conclusion;Abstract;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interests;Authors’ Contributions;Funding Statement;Acknowledgements;Acknowledgments;Supplementary Materials;Reference;Dataset Description;Dataset Files;Abstract;Introduction;Introduction and Materials;Introduction and Methods;Materials;Materials and Methods;Methods;Results;Discussion;Results and Discussion;Discussion and Conclusion;Results and Conclusion;Conclusion;Conclusions;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interest;Authors’ Contributions;Funding Statement;Acknowledgements;Supplementary Materials;References;Appendix;Abbreviations;Preliminaries;Introduction and Preliminaries;Notation;Proof of Theorem;Proofs;Analysis of Results;Examples;Numerical Example;Applications;Numerical Simulation;Model;Model Formulation;Systematic Palaeontology;Nomenclatural Acts;Taxonomic Implications;Experimental;Synthesis;Overview;Characterization;Background;Experimental;Theories;Calculations;Model Verification;Model Implementation;Geographic location;Study Area;Geological setting;Data Collection;Field Testing;Data and Sampling;Dataset;Literature Review;Related Works;Related Work;System Model;Methods and Data;Experimental Results;Results and Analysis;Evaluation;Implementation;Case Presentation;Case Report;Search Terms;Case Description;Case Series;Background;Limitations;Additional Points;Case;Case 1;Case 2 etc.;Concern Details;Retraction Details;Copyright;Related Articles
摘要:
Ischemic stroke is a kind of central nervous disease characterized by high morbidity, high mortality, and high disability. Inflammation and autophagy play important roles in cerebral ischemia/reperfusion (CI/R) injury. The present study characterizes the effects of TLR4 activation on inflammation and autophagy in CI/R injury. An in vivo CI/R rat injury model and an in vitro hypoxia/reoxygenation (H/R) SH-SY5Y cell model were established. Brain infarction size, neurological function, cell apoptosis, inflammatory mediators' levels, and gene expression were measured. Infarction, neurological dysfunction, and neural cell apoptosis were induced in CI/R rats or in H/R-induced cells. The expression levels of NLRP3, TLR4, LC3, TNF-alpha, interleukin-1 (IL-1), interleukin-6 (IL-6), and interleukin-18 (IL-18) clearly increased in I/R rats or in H/R-induced cells, while TLR4 knockdown significantly suppressed NLRP3, TLR4, LC3, TNF-alpha, and interleukin-1/6/18 (IL-1/6/18) in H/R-induced cells, as well as cell apoptosis. These data indicate that TLR4 upregulation induced CI/R injury via stimulating NLRP3 inflammasome and autophagy. Therefore, TLR4, is a potential therapeutic target to improve management of ischemic stroke.
通讯机构:
[Shi, LR ] ;Cent South Univ, Xiangya Hosp, Dept Radiol, 87 Xiangya Rd, Changsha 410005, Peoples R China.
摘要:
PURPOSE: To assess the safety and efficacy of local ablation plus PD-1 inhibitor toripalimab in previously treated unresectable hepatocellular carcinoma (HCC). PATIENTS AND METHODS: In the multicenter, two-stage, and randomized phase 1/2 trial, patients were randomly assigned to receive toripalimab alone (240 mg, every 3 weeks), subtotal local ablation followed by toripalimab starting on post-ablation day 3 (Schedule D3), or on post-ablation day 14 (Schedule D14). The first endpoint of stage 1 was to determine which combination schedule could continue and progression-free survival (PFS) as the primary endpoint for stage 1/2. RESULTS: A total of 146 patients were recruited. During stage 1, Schedule D3 achieved numerically higher objective response rate (ORR) than Schedule D14 for non-ablation lesions (37.5% vs. 31.3%), and was chosen for stage 2 evaluation. For the entire cohort of both stages, patients with Schedule D3 had a significantly higher ORR than with toripalimab alone (33.8% vs. 16.9%; P = 0.027). Moreover, patients with Schedule D3 had improved median PFS (7.1 vs. 3.8 months; P < 0.001) and median overall survival (18.4 vs. 13.2 months; P = 0.005), as compared with toripalimab alone. In addition, six (9%) patients with toripalimab, eight (12%) with Schedule D3, and 4 (25%) with Schedule D14 developed grade 3 or 4 adverse events, and one patient (2%) with Schedule D3 manifested grade 5 treatment-related pneumonitis. CONCLUSIONS: In patients with previously treated unresectable HCC, subtotal ablation plus toripalimab improved the clinical efficacy as compared with toripalimab alone, with an acceptable safety profile.
期刊:
Frontiers in Microbiology,2023年14:1208157 ISSN:1664-302X
通讯作者:
Song, HP;Zeng, Meiyan
作者机构:
[Song, Houpan; Yuan, Chengzhi; Xiong, Meng; Sun, Qifang; Yu, Chang] Hunan Univ Chinese Med, Hunan Prov Key Lab Tradit Chinese Med Diagnost, Changsha, Hunan, Peoples R China.;[Yuan, Chengzhi] Hunan Univ Chinese Med, Sch Med, Changsha, Hunan, Peoples R China.;[Zeng, Meiyan; Song, Houpan; Xiong, Meng; Sun, Qifang; Yu, Chang] Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Hunan, Peoples R China.;[Zhou, Sainan] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.
通讯机构:
[Zeng, MY; Song, HP ] H;Hunan Univ Chinese Med, Hunan Prov Key Lab Tradit Chinese Med Diagnost, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Hunan, Peoples R China.
摘要:
Resistance of Helicobacter pylori (H. pylori) to antibiotics has reached alarming levels worldwide, and the efficacy of the H. pylori eradication treatment has decreased dramatically because of antibiotic resistance. To gain a more comprehensive understanding of the development status, research hotspots, and future trends related to H. pylori antibiotic resistance, we conducted a thorough retrospective analysis via the bibliometrics method. We searched the Science Citation Index Expanded of the Web of Science Core Collection for all pertinent articles on H. pylori antibiotic resistance from 2013 to 2022. R-bibliometrix, CiteSpace, and VOSviewer tools were utilized to depict statistical evaluations in order to provide an unbiased presentation and forecasts in the field. We incorporated a total of 3,509 articles related to H. pylori antibiotic resistance. Publications were inconsistent prior to 2017, but steadily increased after 2017. China generated the most papers and the United States of America received the most citations and the highest H-index. Baylor College of Medicine was the most influential institution in this field, with the highest number of publications and citations, as well as the highest H-index. Helicobacter was the most productive journal, followed by the World Journal of Gastroenterology and Frontiers in Microbiology. The World Journal of Gastroenterology had the highest citation. Graham, David Y was the most productive and cited author. Clarithromycin resistance, prevalence, gastric cancer, quadruple therapy, sequential therapy, 23S rRNA, whole genome sequencing, bismuth, and probiotics appeared with a high frequency in the keywords. The top keywords with the highest citation bursts were vonoprazan, RdxA, biofilm formation, and fatty acid chain. Our research illustrated a multi-dimensional facet and a holistic knowledge structure for H. pylori antibiotic resistance research over the past decade, which can serve as a guide for the H. pylori research community to conduct in-depth investigations in the future.
期刊:
Frontiers in Pharmacology,2023年14:1137609 ISSN:1663-9812
通讯作者:
Liu, BY;Yuan, CY
作者机构:
[Yi, Jian; Tian, Fengming; Xu, Yaqian; Liu, Baiyan; Liu, BY; Chen, Bowei; Ouyang, Yin; Liu, Yingfei] Hunan Univ Chinese Med, Hosp 1, Changsha, Peoples R China.;[Yi, Jian; Tian, Fengming; Xu, Yaqian; Liu, Baiyan; Liu, BY; Chen, Bowei; Ouyang, Yin; Liu, Yingfei] Hunan Univ Chinese Med, MOE Key Lab Res & Translat Prevent & Treatment Maj, Changsha, Peoples R China.;[Yuan, Chunyun] Hunan Hosp Integrated Tradit Chinese & Western Med, Changsha, Peoples R China.;[Yuan, Chunyun] Hunan Acad Chinese Med, Affiliated Hosp, Changsha, Peoples R China.;[Liu, Baiyan; Liu, BY] Hunan Acad Chinese Med, Changsha, Peoples R China.
通讯机构:
[Liu, BY ; Yuan, CY ] H;Hunan Univ Chinese Med, Hosp 1, Changsha, Peoples R China.;Hunan Univ Chinese Med, MOE Key Lab Res & Translat Prevent & Treatment Maj, Changsha, Peoples R China.;Hunan Hosp Integrated Tradit Chinese & Western Med, Changsha, Peoples R China.;Hunan Acad Chinese Med, Affiliated Hosp, Changsha, Peoples R China.
摘要:
Background: Nasopharyngeal carcinoma (NPC) is a usual head and neck malignancy. Guggulsterone (GS) has potential in cancer chemoprophylaxis and treatment, but its therapeutic effect on NPC is unknown. We aimed to explore whether GS could promote the secretion of exosomal circFIP1L1 from NPC cells and its regulatory mechanism.<&wdkj&>Methods: NPC tissues and adjacent tissues were collected from NPC patients. Human nasopharyngeal epithelial cell lines (NP69) and NPC lines (5-8F, CNE1, and HNE1) were used for in vitro experiments. HNE1 cells were treated with GS (20, 40, 60 μmol/L). The expressions of miR-125a-5p and circFIP1L1 were evaluated by qRT-PCR. Cell proliferation and apoptosis abilities were measured by CCK-8 and flow cytometry. HNE1 cell exosomes were extracted and identified, and the levels of VEGFA and VEGFR2 were detected by ELISA. Then miR-125a-5p was knocked down and overexpressed. HUVECs angiogenesis was determined by the tube formation assay. qRT-PCR and Western blot were utilized to evaluate the expressions of VEGFA, MMP-2, MMP-9, and ICAM-1 in HUVECs.<&wdkj&>Results: miR-125a-5p was highly expressed in NPC tissues and cells. GS promoted the secretion of exosomal circFIP1L1 from HNE1 cells to affect HUVECs proliferation and angiogenesis. Overexpression of miR-125a-5p accelerated HUVECs proliferation and angiogenesis. Knocking down miR-125a- 5p inhibited VEGFA expression. In addition, exosomal circFIP1L1 sponged miR-125a-5p, inhibiting the VEGFA pathway to repress HUVECs angiogenesis.<&wdkj&>Conclusions: GS promoted exosomal circFIP1L1 in NPC cells to mediate miR-125a-5p/VEGFA axis affecting tumor angiogenesis.
期刊:
Scandinavian Journal of Rheumatology,2023年52(2):200-207 ISSN:0300-9742
通讯作者:
Wu, B.;Li, C.
作者机构:
[Li, C.; Yu, X.; Wu, B.; Wu, B; Li, C] Chongqing Hosp Tradit Chinese Med, Basic Res Dept Tradit Chinese Med & Pharm, Chongqing 400021, Peoples R China.;[Li, C.; Wu, B.; Wang, J.; Zhu, F.] Chongqing Hosp Tradit Chinese Med, Dept Rheumatol, Chongqing 400021, Peoples R China.;[Yu, X.] Hunan Univ Tradit Chinese Med, Grad Sch, Changsha, Peoples R China.
通讯机构:
[Wu, B; Li, C] C;Chongqing Hosp Tradit Chinese Med, Basic Res Dept Tradit Chinese Med & Pharm, Chongqing 400021, Peoples R China.
摘要:
OBJECTIVE: The aim of this study was to explore the significance of serum CCL28 in Sjögren's syndrome (SS) diagnosis and evaluation. METHOD: The expression of CCL28 mRNA in salivary glands of SS patients from the GEO database was analysed. Serum levels of CCL28 of SS patients, rheumatoid arthritis (RA) patients, systemic lupus erythematosus (SLE) patients, and healthy controls (HCs) were measured by enzyme-linked immunosorbent assay. The serum immunoglobulin A (IgA) levels and the focus score of labial salivary gland (LSG) in patients with SS were also measured, and the correlation between serum IgA levels and serum CCL28 was explored. In addition, the level of serum CCL28 was compared between two subsets of SS patients who were classified by clinical symptoms and laboratory tests. RESULTS: SS patients displayed decreased expression of CCL28 mRNA in salivary glands, accompanying more severe pathological injury. Serum levels of CCL28 in both primary and secondary SS patients were significantly lower than those in the HC group, whereas no significant differences were observed between RA patients or SLE patients and HCs. Compared with RA and SLE patients alone, serum levels of CCL28 were dramatically lower in patients with SS secondary to RA or SLE. No remarkable correlation between serum IgA and CCL28 levels was observed, while the focus score of LSG negatively correlated with serum CCL28 levels. Serum levels of CCL28 were lower in SS patients who had dental caries and thrombocytopenia. CONCLUSION: Serum CCL28 is a useful biomarker in the diagnosis and evaluation of SS.
期刊:
FRONTIERS IN ONCOLOGY,2023年12:7406 ISSN:2234-943X
通讯作者:
Cao, J.;Jiang, X.;Cho, W.C.
作者机构:
[Zhou, Fang] Hunan Univ Chinese Med, Changsha, Peoples R China.;[Zhang, Zhen; Zhou, Fang] Hunan Acad Tradit Chinese Med, Affiliated Hosp, Dept Oncol, Changsha, Peoples R China.;[Zeng, Lingfeng] Prince Wales Hosp, Carol & Richard Yu Peritoneal Dialysis Res Ctr, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China.;[Zeng, Lingfeng] Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci LiHS, Fac Med, Shatin, Hong Kong, Peoples R China.;[Chen, Xi; Zhao, Jinxi; Huang, Xiaobin; Tian, Jintao; Liu, Xujie; Yao, Huayi; Wang, Heping; Pu, Jun] Kunming Med Univ, Dept Neurosurg, Affiliated Hosp 2, Kunming, Peoples R China.
通讯机构:
[Cho, W.C.; Cao, J.] D;[Jiang, X.] K;Department of Clinical Oncology, Hong Kong;Department of Oncology, China;Kunming College of Life Science, China
摘要:
Dual-specificity phosphatase 10 (DUSP10) correlates with inflammation, cytokine secretion, cell proliferation, survival, and apoptosis. However, its role in glioma is unclear. Herein, we sought to examine the expression and the underlying carcinogenic mechanisms of DUSP10 action in glioma. DUSP10 expression in glioma was significantly higher than that in normal brain tissues. High DUSP10 expression indicated adverse clinical outcomes in glioma patients. Increased DUSP10 expression correlated significantly with clinical features in glioma. Univariate Cox analysis showed that high DUSP10 expression was a potential independent marker of poor prognosis in glioma. Furthermore, DUSP10 expression in glioma correlated negatively with its DNA methylation levels. DNA methylation level of DUSP10 also correlated negatively with poor prognosis in glioma. More importantly, DUSP10 expression correlated positively with the infiltration of B cells, CD4+ T cells, CD8+ T cells, neutrophils, macrophages, and dendritic cells in glioma. Gene set enrichment analysis (GSEA) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis confirmed that DUSP10 participated in signaling pathways involved in focal adhesion, TNF cascade, Th17 cell differentiation, and NF-kappa B cascade. Finally, we uncovered that DUSP10 was dramatically upregulated in glioblastoma (GBM) cells and that the knockdown of DUSP10 inhibited glioma cell proliferation and migration. Our findings suggested that DUSP10 may serve as a potential prognostic biomarker in glioma.
作者机构:
[Zhang, Weili; Jin-si-han, E-er-man-bie-ke; Lu, ZH; Lian, Shaopu; Li, Yuan; Lu, Zhenhai; Feng, Cheng; Wang, Hao] Sun Yat Sen Univ Canc Ctr, Dept Colorectal Surg, Guangzhou 510515, Guangdong, Peoples R China.;[He, Meng; He, M] Chinese Acad Med Sci & Peking Union Med Coll, Natl Clin Res Ctr Canc, Canc Hosp, Dept Radiat Oncol,Natl Canc Ctr, Shenzhen 518116, Guangdong, Peoples R China.;[He, Meng; He, M] Chinese Acad Med Sci & Peking Union Med Coll, Shenzhen Hosp, Shenzhen 518116, Guangdong, Peoples R China.;[Chen, QF; Chen, Qifeng] Sun Yat Sen Univ Canc Ctr, Dept Minimally Invas Intervent Therapy, Liver Canc Study & Serv Grp, Guangzhou 510060, Guangdong, Peoples R China.;[Tai, Yi] Sun Yat Senen Univ Canc Ctr, Dept Musculoskeletal Oncol, Guangzhou 510515, Guangdong, Peoples R China.
通讯机构:
[Chen, QF ; Lu, ZH ] S;[He, M ] C;Sun Yat Sen Univ Canc Ctr, Dept Colorectal Surg, Guangzhou 510515, Guangdong, Peoples R China.;Chinese Acad Med Sci & Peking Union Med Coll, Natl Clin Res Ctr Canc, Canc Hosp, Dept Radiat Oncol,Natl Canc Ctr, Shenzhen 518116, Guangdong, Peoples R China.;Chinese Acad Med Sci & Peking Union Med Coll, Shenzhen Hosp, Shenzhen 518116, Guangdong, Peoples R China.
摘要:
Neutrophil extracellular traps (NETs) have been categorized as a form of inflammatory cell death mode of neutrophils (NETosis) involved in natural immunity and the regulation of adaptive immunity. More and more studies revealed the ability of NETs to reshape the tumor immune microenvironment (TIME) by limiting antitumor effector cells, which may impair the efficacy of immunotherapy. To explore whether NETs-related genes make vital impacts on Colon carcinoma (COAD), we have carried out a systematic analysis and showed several findings in the present work. First, we obtained the patient's data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) dataset, aiming to detect two NETs-associated subtypes by consensus clustering. For the purpose of annotating the roles of NETs-related pathways, gene ontology enrichment analyses were adopted. Next, we constructed a 6 novel NETs-related genes score using the Least Absolute Shrinkage and Selection Operator (LASSO) Cox regression model. We found that the NETs risk score was notably upregulated in COAD patient samples, and its levels were notably correlated with tumor clinicopathological and immune traits. Then, according to NETs-associated molecular subtypes and the risk signature, this study compared immune cell infiltration calculated through the estimate, CIBERSORT, TIMER, ssGSEA algorithms, tumor immune dysfunction, as well as exclusion (TIDE). Furthermore, we confirm that MPO(myeloperoxidase) was significantly upregulated in COAD patient samples, and its levels were significantly linked to tumor malignancy and clinic outcome. Moreover, multiplex immunohistochemistry (mIHC) spatial analysis confirmed that MPO was closely related to Treg and PD-1 + Treg in spatial location which suggested MPO may paly an important role in TIME formation. Altogether, the obtained results indicated that a six NETs-related genes prognostic signature was conducive to estimating the prognosis and response of chemo-/immuno-therapy of COAD patients.
作者机构:
[Xia, Zi-An] Cent South Univ, Xiangya Hosp, Dept Integrated Tradit Chinese & Western Med, Changsha 410008, Peoples R China.;[Xia, Zi-An; He, Jiang; Sun, Lunquan] Cent South Univ, Natl Clin Res Ctr Geriatr Disorders, XiangyaHosp, Changsha 410008, Peoples R China.;[Lu, Can] Cent South Univ, Xiangya Hosp, Dept Pathol, Changsha 410078, Peoples R China.;[Pan, Can] Hunan Univ Tradit Chinese Med, Sch Clin Med, Changsha 410208, Peoples R China.;[Li, Jia] Cent South Univ, Xiangya Hosp, Dept Emergency, Changsha 410008, Peoples R China.
通讯机构:
[Sun, LQ ; He, J ; Sun, LQ] ;Cent South Univ, Natl Clin Res Ctr Geriatr Disorders, XiangyaHosp, Changsha 410008, Peoples R China.;Cent South Univ, Xiangya Canc Ctr, Dept Oncol, XiangyaHosp, Changsha 410008, Peoples R China.;Key Lab Mol Radiat Oncol Hunan Prov, Changsha 410008, Peoples R China.;Hunan Int Sci & Technol Collaborat Base Precis Med, Changsha 410008, Peoples R China.
关键词:
Large-scale data analysis;Tumour-infiltrating lymphocytes;CD103;LAG3;Immunotherapy;Chemotherapy
摘要:
Tumour-infiltrating lymphocytes (TILs), including T and B cells, have been demonstrated to be associated with tumour progression. However, the different subpopulations of TILs and their roles in breast cancer remain poorly understood. Large-scale analysis using multiomics data could uncover potential mechanisms and provide promising biomarkers for predicting immunotherapy response. Single-cell transcriptome data for breast cancer samples were analysed to identify unique TIL subsets. Based on the expression profiles of marker genes in these subsets, a TIL-related prognostic model was developed by univariate and multivariate Cox analyses and LASSO regression for the TCGA training cohort containing 1089 breast cancer patients. Multiplex immunohistochemistry was used to confirm the presence of TIL subsets in breast cancer samples. The model was validated with a large-scale transcriptomic dataset for 3619 breast cancer patients, including the METABRIC cohort, six chemotherapy transcriptomic cohorts, and two immunotherapy transcriptomic cohorts. We identified two TIL subsets with high expression of CD103 and LAG3 (CD103+LAG3+), including a CD8+ T-cell subset and a B-cell subset. Based on the expression profiles of marker genes in these two subpopulations, we further developed a CD103+LAG3+ TIL-related prognostic model (CLTRP) based on CXCL13 and BIRC3 genes for predicting the prognosis of breast cancer patients. CLTRP-low patients had a better prognosis than CLTRP-high patients. The comprehensive results showed that a low CLTRP score was associated with a high TP53 mutation rate, high infiltration of CD8 T cells, helper T cells, and CD4 T cells, high sensitivity to chemotherapeutic drugs, and a good response to immunotherapy. In contrast, a high CLTRP score was correlated with a low TP53 mutation rate, high infiltration of M0 and M2 macrophages, low sensitivity to chemotherapeutic drugs, and a poor response to immunotherapy. Our present study showed that the CLTRP score is a promising biomarker for distinguishing prognosis, drug sensitivity, molecular and immune characteristics, and immunotherapy outcomes in breast cancer patients. The CLTRP could serve as a valuable tool for clinical decision making regarding immunotherapy.
作者机构:
[Liu, Xiu; Zou, Junju; Tan, Danni; Yu, Rong; Xiang, Qin; Wu, Yongjun; Shi, Liuyang] Hunan Univ Tradit Chinese Med, Sch Tradit Chinese Med, Changsha 410208, Hunan, Peoples R China.;[Liu, Xiu; Zou, Junju; Tan, Danni; Yu, Rong; Xiang, Qin; Shi, Liuyang] Hunan Univ Chinese Med, Hunan Key Lab Tradit Chinese Med Prescript & Syndr, Changsha 410208, Hunan, Peoples R China.;[Wu, Yongjun] Hunan Univ Tradit Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Zou, Junju] Natl Key Lab Cultivat Base Chinese Med Powder & In, Changsha 410208, Hunan, Peoples R China.
通讯机构:
[Yongjun Wu; Rong Yu] S;School of Traditional Chinese Medicine, Hunan University of Traditional Chinese Medicine, Changsha, Hunan 410208, China<&wdkj&>Hunan University of Traditional Chinese Medicine School of Pharmacy, Changsha, Hunan 410208, China<&wdkj&>School of Traditional Chinese Medicine, Hunan University of Traditional Chinese Medicine, Changsha, Hunan 410208, China<&wdkj&>Hunan Key Laboratory of Traditional Chinese Medicine Prescription and Syndromes Translational Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
摘要:
Non-alcoholic fatty liver disease (NAFLD) pathogenesis is affected by dysbiosis of the gut microbiome and the metabolites it generates. Therefore, restoring the equilibrium between the gut microbiome and the generated metabolites may have therapeutic potential for the syndrome. Zuogui Jiangtang Qinggan Fang (ZGJTQGF) is a Chinese herbal formulation used clinically to treat type 2 diabetic mellitus (T2DM) and fatty liver disease. However, its pharmacological mechanisms have not been well characterized. This work aimed to evaluate the hepatoprotective mechanism of ZGJTQGF in T2DM with NAFLD mice by incorporating gut microbiota, short-chain fatty acids(SCFAs), and metabolomic analysis, and then to provide strong support for clinical treatment of T2DM with NAFLD. The sequencing of 16S rRNA revealed that ZGJTQGF therapy modified the composition and abundance of the gut microbiome, raised the level of SCFAs, and restored the intestinal mucosal barrier. The non-targeted metabolomic analysis of liver tissues identified 212 compounds, of which108 were differentially expressed between the HFD and ZGJTQGF groups. Moreover, L-glutamic acid, L-Phenylalanine, Glycine, Taurine, Deoxycholic acid, and citric acid levels were also considerably altered by ZGJTQGF. Our findings suggest that ZGJTQGF ameliorates HFD-induced hepatic steatosis by modulating the gut microbiota composition and its metabolites and boosting the levels of SCFAs. More notably, ZGJTQGF may be a promising medication for preventing and treating NAFLD.
作者机构:
[Zhong, Dayuan; Liu, Pingwen; Liu, Deliang; Ou, Xueming] Guangzhou Univ Chinese Med, Guangdong Prov Hosp Integrated Tradit Chinese & We, Foshan 528200, Peoples R China.;[Zheng, Mengxue; Gan, Zhenyu; Luo, Hongsheng] Guangzhou Univ Chinese Med, Guangzhou 510006, Peoples R China.;[Deng, Yihui; Li, Lan] Hunan Univ Tradit Chinese Med, Changsha 410208, Peoples R China.;[Cheng, Hui] Jinan Univ, Guangzhou 510632, Peoples R China.;[Li, Huanjie] Foshan Hosp Tradit Chinese Med, Foshan 528099, Peoples R China.
通讯机构:
[Zhong, DY ] G;Guangzhou Univ Chinese Med, Guangdong Prov Hosp Integrated Tradit Chinese & We, Foshan 528200, Peoples R China.
关键词:
Blood-brain barrier;Nanoparticle drug delivery system;Nanomedicine;Bibliometric analysis;Central nervous system
摘要:
BACKGROUND: The blood-brain barrier (BBB) is a natural physiological barrier that protects the central nervous system from foreign substances and limits the delivery of drugs to the brain. Nanotechnology has opened up new possibilities for drug delivery in the brain. Over several decades, various Nanoparticle Drug Delivery Systems (NDDS) that can cross the BBB have been developed for targeted delivery in the brain. To gain a comprehensive understanding of the current research hotspots and trends of NDDS across the BBB, this paper employs bibliometric analysis of articles published in the core database of Web of Science (WOS) from 1996 to 2022. METHOD: A search for relevant research literature on NDDS that can cross the BBB was conducted in the Web of Science database, covering the period from 1996 to 2022. The Bibliometrix R-4.0 software package was used to analyze data related to the countries of publication, research institutions, journals, citations, and keywords. The analysis aimed to identify the co-occurrence of keywords in the documents, including their titles and abstracts. Additionally, cooperative network analyses of authors, institutions, and countries of publication were conducted. RESULTS: A total of 436 articles were analyzed, originating from 174 journals and 13 books, with the majority published in Q1 and Q2 journals. Contributors from 53 countries or regions participated in the publication of these articles, with China, the United States, and India having the highest number of articles by correspondent authors, and China, the United States, and Germany being the most cited countries. Fudan University, Hacettepe University, and Sichuan University were the top three institutions with the most publications. Among the 436 articles analyzed, 1337 keywords and 1450 keywords plus were identified. Factor analysis grouped the keywords plus into two categories: drug delivery systems, polymeric nanoparticles, gold nanoparticles, transferrin, and others, and drug, delivery, efficiency, expression, and mechanism. CONCLUSION: The research on NDDS that can cross the BBB is gradually receiving attention, and the recognition and cooperation in this field have increased.
通讯机构:
[Guo, ZH ] H;Hunan Univ Chinese Med, 300 Xue Shi Rd, Changsha 410208, Peoples R China.
关键词:
bile acids;gut microbiota;heart failure;metabolites
摘要:
Heart failure (HF) is the terminal manifestation of various cardiovascular diseases. Recently, accumulating evidence has demonstrated that gut microbiota are involved in the development of various cardiovascular diseases. Gut microbiota and their metabolites might play a pivotal role in the development of HF. However, previous studies have rarely described the complex role of gut microbiota and their metabolites in HF. In this review, we mainly discussed bile acids (BAs), the metabolites of gut microbiota. We explained the mechanisms by which BAs are involved in the pathogenesis of HF. We also discussed the use of gut microbiota and BAs for treating HF in Chinese medicine, highlighting the advantages of Chinese medicine in treating HF.
期刊:
Biochemical Systematics and Ecology,2023年109:104662 ISSN:0305-1978
通讯作者:
Wang, Wei;Jian, YQ
作者机构:
[Wang, Wei; Yang, Yupei; Li, Wenchu; Jiang, Sai; Jian, Yuqing; Guo, Tingsi; Wu, Juanjiang; Yang, Yong] Hunan Univ Chinese Med, Innovat Mat Med Res Inst, Sch Pharm, TCM & Ethnomedicine Innovat & Dev Int Lab, Changsha 410208, Hunan, Peoples R China.
通讯机构:
[Wang, W; Jian, YQ ] H;Hunan Univ Chinese Med, Innovat Mat Med Res Inst, Sch Pharm, TCM & Ethnomedicine Innovat & Dev Int Lab, Changsha 410208, Hunan, Peoples R China.
摘要:
Tumor metastasis is the leading cause of mortality among advanced cancer patients. Understanding its mechanisms and treatment strategies is vital for clinical application. Arginine methylation, a post-translational modification catalyzed by protein arginine methyltransferases (PRMTs), is implicated in diverse physiological processes and disease progressions. Previous research has demonstrated PRMTs' involvement in tumor occurrence, progression, and metastasis. This review offers a comprehensive summary of the relationship between PRMTs, prognosis, and metastasis in various cancers. Our focus centers on elucidating the molecular mechanisms through which PRMTs regulate tumor metastasis. We also discuss relevant clinical trials and effective PRMT inhibitors, including chemical compounds, long non-coding RNA (lncRNA), micro-RNA (miRNA), and nanomaterials, for treating tumor metastasis. While a few studies present conflicting results, the overall trajectory suggests that inhibiting arginine methylation exhibits promise in curtailing tumor metastasis across various cancers. Nonetheless, the underlying mechanisms and molecular interactions are diverse. The development of inhibitors targeting arginine methylation, along with the progression of clinical trials, holds substantial potential in the field of tumor metastasis, meriting sustained attention.
摘要:
Background: Among the 382 million diabetic patients worldwide, approximately 30% experience neuropathy, and one-fifth of these patients eventually develop diabetes cognitive impairment (CI). However, the mechanism underlying diabetes CI remains unknown, and early diagnostic methods or effective treatments are currently not available.Objective: This study aimed to explore the risk factors for CI in patients with type 2 diabetes mellitus (T2DM), screen potential therapeutic drugs for T2DM-CI, and provide evidence for preventing and treating T2DM-CI.Methods: This study focused on the T2DM population admitted to the First Affiliated Hospital of Hunan College of Traditional Chinese Medicine and the First Affiliated Hospital of Hunan University of Chinese Medicine. Sociodemographic data and clinical objective indicators of T2DM patients admitted from January 2018 to December 2022 were collected. Based on the Montreal Cognitive Assessment (MoCA) Scale scores, 719 patients were categorized into two groups, the T2DM-CI group with CI and the T2DM-N group with normal cognition. The survey content included demographic characteristics, laboratory serological indicators, complications, and medication information. Six machine learning algorithms were used to analyze the risk factors of T2DM-CI, and the Shapley method was used to enhance model interpretability. Furthermore, we developed a graph neural network (GNN) model to identify potential drugs associated with T2DM-CI.Results: Our results showed that the T2DM-CI risk prediction model based on Catboost exhibited superior performance with an area under the receiver operating characteristic curve (AUC) of 0.95 (specificity of 93.17% and sensitivity of 78.58%). Diabetes duration, age, education level, aspartate aminotransferase (AST), drinking, and intestinal flora were identified as risk factors for T2DM-CI. The top 10 potential drugs related to T2DM-CI, including Metformin, Liraglutide, and Lixisenatide, were selected by the GNN model. Some herbs, such as licorice and cuscutae semen, were also included. Finally, we discovered the mechanism of herbal medicine interventions in gut microbiota.Conclusion: The method based on Interpreting AI and GNN can identify the risk factors and potential drugs associated with T2DM-CI.
期刊:
Journal of Clinical Nursing,2023年32(11-12):2399-2409 ISSN:0962-1067
通讯作者:
Chengwei Fu PhD<&wdkj&>Juan Yuan MD
作者机构:
[Chen, Wenzhen; Yuan, Juan] Anhui Univ Chinese Med, Sch Nursing, 103 Meishan Rd, Hefei 230012, Anhui, Peoples R China.;[Fu, Chengwei] Guangzhou Univ Chinese Med, Clin Med Sch 2, 12 Airport Rd, Guangzhou 510080, Guangdong, Peoples R China.;[Wu, Boyu] Hunan Univ Chinese Med, Changsha, Peoples R China.;[Zhou, Hong] Wuhan 1 Hosp, Wuhan, Peoples R China.;[Chen, Erfei] Univ Sci & Technol China, Sch Software Engn, Hefei, Peoples R China.
通讯机构:
[Chengwei Fu PhD; Juan Yuan MD] T;The Second Clinical Medical School, Guangzhou University of Chinese Medicine Guangzhou, China<&wdkj&>The School of Nursing, Anhui University of Chinese Medicine, Hefei, China