通讯机构：
[Bo-Hoh Xia] K;Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, Hunan University of Chinese Medicine, Changsha 410208, PR China
摘要：
Our preliminary results suggested a favorable therapeutical effect of essential oil from Prunella vulgaris L. (PVO) on pelvic inflammation. In this study, we further analyzed the chemical components of PVO through gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS), and its anti-bacterial, anti-inflammatory, and immunoregulatory properties. Fifty-six chemical com-ponents were identified with GC-MS, most of which were fatty acids. The positive and negative ion modes of LC-MS were used to detect 48 and 37 compounds with content above 0.1%, respectively, most of which were terpenoids and lipids. The anti-bacterial experiment revealed that PVO could strongly inhibit both gram -neg-ative and gram-positive bacteria. It had a significant inhibiting effect on influenza virus neuraminidase, with an inhibition ratio of over 50%. Besides, PVO dramatically inhibited the NO release and the levels of IL-6 in RAW264.7 cells induced by LPS. Moreover, immunological results indicated that PVO could synergistically facilitate concanavalin A (ConA) to stimulate the secretion of Th1 and Th2 cytokines from the splenic lym-phocytes of mice but also regulate the Th1/Th2 balance to exert dual immunoregulatory effects. Generally, these results proved the anti-bacterial, anti-inflammatory, and immunoregulatory effects of PVO, thus pro-viding a reference for the application of PVO as a food or drug.(c) 2022 Published by Elsevier B.V. on behalf of SAAB.
通讯机构：
College of Acupuncture, Massage and Rehabilitation, Hunan University of Chinese Medicine, Changsha, China
摘要：
The latest spectrum of moxibustion disease shows that primary dysmenorrhea is a high-frequency symptom of moxibustion and that it is the dominant clinical disease. In the specific treatment methods, all types of moxibustion methods have been widely used, such as thermal, thunder fire, partitioned, and spreading moxibustion. Moxibustion plays a therapeutic role through its four mechanisms of action: heat, light, moxa smoke, and drug effects. The mechanism of moxibustion treatment for primary dysmenorrhea focuses on adjusting endocrine hormones, regulating immune function and neuro-related factors, and improving uterine microcirculation. In this study, based on the clinical evidence of different moxibustion methods for treating primary dysmenorrhea, the design model, intervention characteristics, and clinical outcomes were analyzed. Meanwhile, the brain effect mechanisms of different imaging methods were summarized from the perspective of neuroimaging. It was pointed out that the left anterior cingulate gyrus, left inferior parietal angular gyrus, and left superior gyrus may be the analgesic brain regions that regulate sensory, emotional, and cognitive aspects. Moreover, the neural circuits involved can be inferred: the frontal cortex-basal ganglia (the pea nucleus)-cerebral cortex, which mediates motivation and emotional drive, and the parietal lobe-basal ganglia-limbic lobe-frontal lobe, which is involved in neurotransmitter transport and emotional regulation and behavioral expression. There are still problems and deficiencies in studies on the mechanism of moxibustion treatment for primary dysmenorrhea. Studies should be strengthened on how moxibustion produces an effect. Attention should be paid to exploring how the spectrum range and peak in the light effect of moxibustion treat primary dysmenorrhea. Studies assessing the mechanisms of moxibustion treatment for primary dysmenorrhea should be conducted to provide an experimental basis and evidence-based medical evidence for clinical treatment.
摘要：
OBJECTIVE: Women have a higher lifetime risk of stroke than men and are more likely to die from it. Ferroptosis is a recently discovered form of programmed cell death implicated in many diseases. The role of ferroptosis-related genes in the diagnosis, prognosis. and treatment of elderly women with ischemic stroke (IS) requires additional clarification. This paper aimed to screen ferroptosis-related genes associated with IS in elderly women and to identify hub genes and candidate drugs. MATERIALS AND METHODS: Ferroptosis-related differentially expressed genes (DEGs) in elderly women with IS were identified by bioinformatics analysis of the GSE22255 and ferroptosis-related gene datasets. Subsequently, ferroptosis-related hub genes were used to predict targeted miRNA, construct the miRNA-mRNA network, and identify candidate drugs. RESULTS: Eleven ferroptosis-related DEGs were identified in elderly women with IS vs. controls. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis revealed that the 11 genes were mainly enriched in the IL-17. TNF, and NF-kappa B signaling pathways. Moreover. the hub genes suggested 10 ferroptosis-related biomarkers for IS, including SOCS1, IFNG, TNFAIP3, IL1B, IL-6, PTGS2, DDIT3, CXCL2, NFE2L2. and ATF3. Furthermore, our findings revealed the miRNA-mRNA network of the hub genes and identified candidate drugs. 10 potential therapeutic compounds, especially estradiol CTD 00005920, corresponded to the 10 key genes which could be targets for IS treatment in elderly women. CONCLUSIONS: Our results suggested ferroptosis-related DEGs (SOCS1, 1FNG, TNFAIP3, IL1B, IL-6, PTGS2, DDIT3, CXCL2, NFE2L2. and ATF3) as potential biomarkers for IS diagnosis, prognosis, and treatment, providing additional evidence of the important role of ferroptosis in IS in elderly women.
期刊：
Frontiers in Pharmacology,2022年13:3205 ISSN：1663-9812
作者机构：
[Wang, Huiling; Liang, Jingyan; Chen, Yu; Mo, Chao] Guangxi Univ Chinese Med, Grad Sch, Nanning, Peoples R China.;[Mo, Chao] Guangxi Univ Chinese Med, Affiliated Hosp 1, Dept Nephrol, Nanning, Peoples R China.;[Zhao, Jie] Hunan Univ Chinese Med, Grad Sch, Changsha, Peoples R China.;[Huang, Guodong] Guangxi Univ Chinese Med, Guangxi Int Zhuang Med Hosp, Dept Nephrol, Nanning, Peoples R China.
关键词：
Traditional Chinese Medicine;exosome;modulator;Delivery carrier;biomarker
摘要：
Exosomes are small extracellular vesicles and play an essential role in the mediation of intercellular communication both in health and disease. Traditional Chinese medicine (TCM) has historically been used to maintain human health and treat various diseases up till today. The interplay between exosomes and TCM has attracted researchers' growing attention. By integrating the available evidence, TCM formulas and compounds isolated from TCM as exosome modulators have beneficial effects on multiple disorders, such as tumors, kidney diseases, and hepatic disease, which may associate with inhibiting cells proliferation, anti-inflammation, anti-oxidation, and attenuating fibrosis. Exosomes, a natural delivery system, are essential in delivering compounds isolated from TCM to target cells or tissues. Moreover, exosomes may be the potential biomarkers for TCM syndromes, providing strategies for TCM treatment. These findings may provide a novel insight into TCM from exosomes and serve as evidence for better understanding and development of TCM.
摘要：
Hyperlipidemia, the most common form of dyslipidemia, is the main source of cardiovascular disorders, characterized by elevated level of total cholesterol (TC), triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C) with high-density lipoprotein cholesterol (HDL-C) in peripheral blood. It is caused by a defect in lipid metabolism in the surface of Apoprotein C-II or a defect in lipoprotein lipase activity as well as reported in genetic, dietary and environmental factors. Several electronic databases were investigated as information sources, including Google Scholar, PubMed, Web of Science, Scopus, ScienceDirect, SpringerLink, Semantic Scholar, MEDLINE and CNKI Scholar. The current review focused on the risk factors of dyslipidemia, synthetic medication with their side effects and different types of medicinal plants having significant potential for the management of hyperlipidemia. The management of hyperlipidemia mostly involves a constant decrease in lipid level using different remedial drugs like statin, fibrate, bile acid sequestrates and niacin. However, this extensive review suggested that the consequences of these drugs are arguable, due to their numerous adverse effects. The selected parts of herb plants are used intact or their extracts containing active phytoconstituents to regulate the lipids in blood level. It was also noted that the Chinese herbal medicine and combination therapy is promising for the lowering of hyperlipidemia. This review intends to provide a scientific base for future endeavors, such as in-depth biological and chemical investigations into previously researched topics.
期刊：
Frontiers in Immunology,2022年13:949746 ISSN：1664-3224
通讯作者：
Ge, J.
作者机构：
[Xu, Hao; Yang, Kailin; Wang, Shanshan; Ge, Jinwen; Yuan, Xiao] Hunan Univ Chinese Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha, Peoples R China.;[Chen, Junpeng] Hunan Univ Sci & Technol, Sch Mech Engn, Xiangtan, Peoples R China.;[Zhang, Tianqing] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Cardiovasc Med, Hengyang, Peoples R China.;[Ge, Anqi] Hunan Univ Chinese Med, First Hosp, Changsha, Peoples R China.;[Zeng, Liuting] Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, Dept Rheumatol & Clin Immunol, Beijing, Peoples R China.
通讯机构：
[Ge, J.] K;Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, China
关键词：
nonalcoholic fatty liver disease;Systematic review;Meta-analysis;Polyphenol - Rich Extract of leaves of Vernonia amygdalina (PEVA);Dietary polyphenol intervention
摘要：
The intake of moderate oils and fats is necessary to maintain the body's energy balance, and the fatty acid composition of different oils and fats varies in their nutrition and function. The study aimed to investigate the effects of lard and vegetable blend oil on gut microbiota, intestinal enzyme activities, and blood routine. Kunming mice were assigned to the three groups: (1) Control group (CK) was gavage administration with distilled water, (2) Plant oil group (ZWY) was gavage administration with edible vegetable blend oil, (3) Lard group (DWY) was gavage administration with lard. After 42 days, microbiological, digestive enzymes, and blood routine were performed. Compared with the CK group, Escherichia coli, Lactobacilli, and Bifidobacteria were significantly decreased (p < 0.05), the activities of protease, cellulase, amylase, and xylanase were markedly reduced (p < 0.05), the hemoglobin was significantly increased (p < 0.05) in the ZWY group and DWY groups, and the hematocrit was increased in the ZWY group (p < 0.05), while other routine blood indices were increased (p > 0.05). Compared to the ZWY group, the activity of cellulase and amylase were significantly increased (p < 0.05), the intestinal microorganism and the routine blood indexes had no significant difference in the DWY group. Lard and vegetable blend oil diet affected the composition of the intestinal microorganisms, and the functions of digestive enzymes. Meanwhile, the levels of digestive enzymes may be correlated with the intestinal microbiota.
摘要：
A pair of 3,4-seco-cycloartane triterpenoid isomers with a rare peroxy bridge, namely, xuetonins A and B (1 and 2), four new lignans xuetonlignans A-D (3-6), a new sesquiterpene xuetonpene (7), and a new natural product xuetonin C (8), along with 43 known compounds, were obtained from the leaves of Tujia ethnomedicine, Kadsura heteroclita. Their structures and configurations were determined with the help of a combination of 1D- and 2D-NMR, HRESIMS spectra, electronic circular dichroism (ECD), and X-ray diffraction data. Compounds 2, 10, 13-15, and 17-19 showed moderate-to-potent activity against rheumatoid arthritis fibroblast-like synoviocytes (RAFLS) with IC50 values of 19.81 +/- 0.26, 12.73 +/- 0.29, 5.70 +/- 0.24, 9.25 +/- 0.79, 5.66 +/- 0.52, 11.91 +/- 0.44, 13.22 +/- 0.27, and 15.94 +/- 0.36 mu M, respectively. Furthermore, compounds 22, 25, and 31 exhibited significant hepatoprotective effects against N-acetyl-p-aminophenol (APAP)-induced toxicity in HepG2 cells at 10 mu M, and the cell viability increased by 12.93, 25.23, and 13.91%, respectively, compared with that in the model group (cf. bicyclol, 12.60%).
摘要：
Soybean isoflavones (SIs) are widely found in food and herbal medicines. Although the pharmacological activities of SIs have been widely reported, their effects on the intestinal microecology of normal hosts have received little attention. Five-week-old Kunming (KM) mice were administered SIs (10 mg/kg/day) for 15 days. Food intake, body weight, and digestive enzyme activity were measured. Small intestine microbiota, including lumen-associated bacteria (LAB) and mucosa-associated bacteria (MAB), were analyzed using 16S ribosomal ribonucleic acid (16S rRNA) gene sequencing. Short-chain fatty acids (SCFAs) were analyzed using gas chromatography-mass spectrometry (GC-MS). The results showed that the mice that consuming SIs showed a higher food intake but a lower body weight gain rate than that of normal mice. Sucrase, cellulase, and amylase activities reduced, while protease activity increased after SIs intervention. Moreover, SIs increased the intestinal bacterial diversity in both LAB and MAB of normal mice. The composition of LAB was more sensitive to SIs than those of MAB. Lactobacillus, Adlercreutzia, Coprococcus, Ruminococcus, Butyricicoccus, and Desulfovibrio were the differential bacteria among the LAB of mice treated with SIs. In addition, acetic acid, valeric acid, isobutyric acid, isovaleric acid, and caproic acid decreased, while butyric acid and propionic acid increased in the mice treated with SIs. Taken together, SIs are beneficial for weight control, even in short-term interventions. The specific mechanism is related to regulating the gut microbiota, changing digestive enzyme activities, and further affecting carbohydrate absorption and metabolism.
作者：
Zhang, Shuo;Zhu, Neng;Li, Hong Fang;Gu, Jia;Zhang, Chan Juan;...
期刊：
Stem Cell Research & Therapy,2022年13(1):1-15 ISSN：1757-6512
通讯作者：
Qin, Li
作者机构：
[Zhang, Shuo; Zhang, Chan Juan; Li, Hong Fang; Liao, Duan Fang; Gu, Jia; Qin, Li] Hunan Univ Chinese Med, Sch Pharm, Lab Stem Cell Regulat Chinese Med & Its Applicat, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.;[Zhu, Neng] Hunan Univ Chinese Med, Dept Urol, Hosp 1, Changsha, Peoples R China.;[Qin, Li] Hunan Univ Chinese Med, Inst Key Lab Vasc Biol & Translat Med Hunan Prov, Changsha, Peoples R China.;[Qin, Li] Hunan Univ Chinese Med, Hunan Prov Engn Res Ctr Bioact Subst Discovery Tr, Changsha, Peoples R China.
通讯机构：
[Qin, Li] L;Laboratory of Stem Cell Regulation With Chinese Medicine and Its Application, School of Pharmacy, Hunan University of Chinese Medicine, 300 Xueshi Road, Hanpu Science and Education District, 410208, Changsha, Hunan, People's Republic of China.;Institutional Key Laboratory of Vascular Biology and Translational Medicine in Hunan Province, Hunan University of Chinese Medicine, Changsha, China.;Hunan Province Engineering Research Center of Bioactive Substance Discovery of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China.
摘要：
Cancer stem cells (CSCs) are a subpopulation of cancer cells with stem cell properties that sustain cancers, which may be responsible for cancer metastasis or recurrence. Lipid rafts are cholesterol- and sphingolipid-enriched microdomains in the plasma membrane that mediate various intracellular signaling. The occurrence and progression of cancer are closely related to lipid rafts. Emerging evidence indicates that lipid raft levels are significantly enriched in CSCs compared to cancer cells and that most CSC markers such as CD24, CD44, and CD133 are located in lipid rafts. Furthermore, lipid rafts play an essential role in CSCs, specifically in CSC self-renewal, epithelial-mesenchymal transition, drug resistance, and CSC niche. Therefore, lipid rafts are critical regulatory platforms for CSCs and promising therapeutic targets for cancer therapy.
期刊：
Journal of Healthcare Engineering,2022年2022 ISSN：2040-2295
通讯作者：
Zhang, W
作者机构：
[Li, Junxi; Yang, Renyi; Zhang, Wei; Fu, Xinying] Hunan Univ Chinese Med, Changsha 410000, Hunan, Peoples R China.
通讯机构：
[Zhang, W ] H;Hunan Univ Chinese Med, Changsha 410000, Hunan, Peoples R China.
摘要：
Atherosclerosis is a chronic inflammatory disease of the arterial wall and the main cause of cardiovascular disease and cerebrovascular disease. In recent years, the mortality rate of atherosclerotic diseases has become higher and higher. This article aims to study the dysregulation of atherosclerotic vascular endothelial secretion and smooth muscle cell proliferation, and put forward and practice the pathological research of atherosclerotic disease. This article describes in detail atherosclerosis, endothelial dysfunction, and smooth muscle cell proliferation, and studies the causes of atherosclerosis. Research results indicate that atherosclerotic vascular endothelial dysfunction also has a great influence on the proliferation of smooth muscle cells. Many genes and environmental factors can regulate the functions of endothelial cells, vascular smooth muscle cells, and mononuclear macrophages and affect the formation of atherosclerosis. At the same time, diabetes, hypertension, hyperlipidemia, obesity, etc. are the main causes of atherosclerosis. The number of patients with cardiovascular and cerebrovascular diseases dying from atherosclerosis in the country is increasing, and the proportion is close to 30%.
期刊：
Frontiers in Physiology,2022年13:937737 ISSN：1664-042X
作者机构：
[Zhou, Zhihuan; Chen, Rui] Tianjin Univ Tradit Chinese Med, Coll Tradit Chinese Med, Tianjin, Peoples R China.;[Chen, Ting] Hunan Univ Chinese Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha, Peoples R China.;[Yang, Xintong; Wang, Yi; Ding, Xinya; Zhang, Han; Song, Zhihui; Feng, Wanying] Tianjin Univ Tradit Chinese Med, Inst Tradit Chinese Med, Tianjin, Peoples R China.;[Li, Bin; Wang, Xianliang] Tianjin Univ Tradit Chinese Med, Natl Clin Res Ctr Chinese Med Acupuncture & Moxibu, Teaching Hosp 1, Tianjin, Peoples R China.;[Zhang, Han] Tianjin Univ Tradit Chinese Med, Tianjin State Key Lab Modern Chinese Med, Tianjin, Peoples R China.
关键词：
Tangzhiqing;Inflammasomes;pyroptosis;Atherosclerosis;Chinese traditional medicine
摘要：
Tangzhiqing formula (TZQ) is a traditional Chinese medicine prescribed to treat glucose and lipid metabolism disorders. A significant effect of TZQ on diabetes and hyperlipidemia has been demonstrated, but its effect on atherosclerosis (AS) remains unknown. This study combines pyroptosis with metabolomics to elucidate the effect and mechanism of TZQ on AS. A model of AS was developed using ApoE(-/-) mice fed a high-fat diet for 8 weeks. After 6 weeks of atorvastatin (Ator) or TZQ treatment, aortic lumen diameter, aortic lesion size, serum lipid profile, cytokines, and Nod-like receptor protein 3 (NLRP3) inflammasome-mediated pyroptosis were analyzed. Serum metabolomics profiles were obtained to examine the effect of TZQ on AS and the correlation between pyroptosis and metabolites was further analyzed. As a result, TZQ significantly reduced the diameter of the common carotid artery during diastole and the blood flow velocity in the aorta during systole; reduced blood lipid levels, arterial vascular plaques, and the release of inflammatory cytokines; and inhibited the NLRP3 inflammasome-mediated pyroptosis. According to metabolomics profiling, TZQ is engaged in the treatment of AS via altering arachidonic acid metabolism, glycerophospholipid metabolism, steroid hormone production, and unsaturated fatty acid biosynthesis. The cytochrome P450 enzyme family and cyclooxygenase 2 (COX-2) are two major metabolic enzymes associated with pyroptosis.
期刊：
Frontiers in Pharmacology,2022年13:3280 ISSN：1663-9812
作者机构：
[Du, Ke; Peng, Caiwang; Sun, Yang; Li, Hengli; Zhao, Fengyan; Liu, Fang] Hunan Univ Chinese Med, Sch Pharm, Changsha, Peoples R China.;[Peng, Caiwang; Sun, Yang; Li, Hengli; Zhao, Fengyan; Liu, Fang] Ctr Standardizat & Funct Engn Tradit Chinese Med H, Changsha, Peoples R China.;[Peng, Caiwang; Sun, Yang; Li, Hengli; Zhao, Fengyan; Liu, Fang] Educ Dept Hunan Prov, Key Lab Modern Res TCM, Changsha, Peoples R China.
关键词：
Traditional Chinese Medicine(TCM);cerebral ischemia;ferroptosis;Apoptosis;Autophagy
摘要：
Traditional Chinese medicine (TCM) has attracted a great deal of attention in the treatment of cerebral ischemia is credited with the remarkable neuroprotective effects. However, the imperfect functional mechanism of TCM is a major obstacle to their application. Many studies have been conducted to illustrate the pathophysiology of post-ischemic cerebral ischemia by elucidating the neuronal cell death pathway. Meanwhile, a new type of cell death, ferroptosis, is gradually being recognized in various diseases and is becoming a new pathway of therapeutic intervention strategy to solve many health problems. Especially since ferroptosis has been found to be closely involved into the pathogenesis of cerebral ischemia, it has been considered as a key target in the treatment of cerebral ischemia. Therefore, this paper reviews the latest research findings about the treatment of cerebral ischemia with TCM focused on ferroptosis as a target. Also, in order to explores the possibility of a new approach to treat cerebral ischemia with TCM, we discusses the correlation between ferroptosis and other cell death pathways such as apoptosis and autophagy, which would provide references for the following researches.
期刊：
IEEE Transactions on Industrial Informatics,2022年18(7):4969-4980 ISSN：1551-3203
通讯作者：
Xiong, N.
作者机构：
[Liu, Anfeng; Mo, Wen] Cent South Univ, Sch Comp Sci & Engn, Changsha 410083, Peoples R China.;[Liu, Wei] Hunan Univ Chinese Med, Sch Informat, Changsha 410208, Peoples R China.;[Huang, Guosheng] Hunan First Normal Univ, Sch Informat Sci & Engn, Changsha 410205, Peoples R China.;[Xiong, Neal N.] Sul Ross State Univ, Dept Comp Sci & Math, Alpine, TX 79830 USA.;[Zhang, Shaobo] Hunan Univ Sci & Technol, Sch Comp Sci & Engn, Xiangtan 411201, Peoples R China.
通讯机构：
[Xiong, N.] D;Department of Computer Science and Mathematics, United States
关键词：
Cloud computing;Data collection;Reliability;Internet of Things;Routing;Smart grids;Monitoring;Cloud computing;Internet of Things (IoTs);reliable trust computing;security of data collection;unmanned aerial vehicles
摘要：
Large number of Internet of Things (IoT) applications with intelligent sensing devices (ISDs) are combined with cloud computing to collect and process data more efficiently. However, ISDs are vulnerable to various attacks. Compromised devices may maliciously provide unreliable data to the cloud, causing damage to IoT applications. Therefore, it is a critical issue to design an effective mechanism to ensure the security of data collection in cloud computing. In this article, a cloud-assisted reliable trust computing (CRTC) scheme is proposed to identify the trust of ISDs at low cost, providing high-quality data for IoT applications. The CRTC scheme mainly includes the following parts: First, a reliable approach of obtaining the real data of ISDs at a low cost is proposed to identify the trust of ISDs. In the proposed method, ISDs fits the forwarding packets information to form inspection information (II) with a small amount of data and then sends II to inspection nodes, effectively reducing the cost of routing II. Second, an effective method of trust computing is given to evaluate the trustworthiness of ISDs based on the data and II collected by unmanned aerial vehicles (UAV). Then, the aggregators are selected from high-trust ISDs to ensure secure data collection. Third, to obtain more reliable trust at lower cost, a trajectory optimization algorithm for UAV is proposed to collect as much II as possible and reduce the moving distance. Theoretical analysis and experimental results show that the proposed CRTC scheme is superior to previous strategies in terms of the success rate of data collection, the speed of identifying the trust of ISDs, and the UAV's trajectory distance.
作者机构：
[Zeng, Liuting; Xu, Hao; Zeng, Jinsong; Yang, Kailin; Wang, Shanshan; Ge, Anqi; Ge, Jinwen; Yuan, Xiao] Hunan Univ Chinese Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha, Peoples R China.;[Zeng, Liuting] Chinese Academy of Medical Sciences & Peking Union, Peking Union Med Coll Hosp, Natl Clin Res Ctr Dermatol & Immunol Dis NCRC DID, Key Lab Rheumatol & Clin Immunol Minist Educ,Dept, Beijing, Peoples R China.;[Zeng, Jinsong; Ge, Anqi] Hunan Univ Chinese Med, Hosp 1, Changsha, Hunan, Peoples R China.
通讯机构：
[Jinwen Ge] K;Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine, Changsha, China
关键词：
Ischemic stroke;Ferroptosis;Oxidative stress;Natural pharmacological active component
摘要：
Cerebrovascular diseases, such as ischemic stroke, pose serious medical challenges worldwide due to their high morbidity and mortality and limitations in clinical treatment strategies. Studies have shown that reactive oxygen species (ROS)-mediated inflammation, excitotoxicity, and programmed cell death of each neurovascular unit during post-stroke hypoxia and reperfusion play an important role in the pathological cascade. Ferroptosis, a programmed cell death characterized by iron-regulated accumulation of lipid peroxidation, is caused by abnormal metabolism of lipids, glutathione (GSH), and iron, and can accelerate acute central nervous system injury. Recent studies have gradually uncovered the pathological process of ferroptosis in the neurovascular unit of acute stroke. Some drugs such as iron chelators, ferrostatin-1 (Fer-1) and liproxstatin-1 (Lip-1) can protect nerves after neurovascular unit injury in acute stroke by inhibiting ferroptosis. In addition, combined with our previous studies on ferroptosis mediated by natural compounds in ischemic stroke, this review summarized the progress in the regulation mechanism of natural chemical components and herbal chemical components on ferroptosis in recent years, in order to provide reference information for future research on ferroptosis and lead compounds for the development of ferroptosis inhibitors.
通讯机构：
[Kangping Xu] X;Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China<&wdkj&>Hunan QingYa Health Service Limited Company, Changsha 410083, China<&wdkj&>The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, China<&wdkj&>Hunan Key Laboratory of Diagnostic and Therapeutic Drug Research for Chronic Diseases, Central South University, Changsha 410013, China
摘要：
Abstract: Five new furofurans lignans, Brasesquilignan A–E (1–5), were isolated from the aqueous ethanol extract of Selaginella braunii Baker. Their structures were elucidated by extensive analysis of NMR and HRESIMS data. Their absolute configurations were determined by CD spectra, enzymatic hydrolysis, and GCMS analysis. Furthermore, all compounds were evaluated for anti-proliferative activities against various human cancer cellsin vitro. Compounds 2 and 3 exhibited weak inhibitorypotency against five human cancer cells. Keywords: Selaginellaceae; Selaginella braunii Baker; furofurans lignans; anti-proliferativeactivity
摘要：
The drug resistance of chemotherapy is a major challenge to overcome for antineoplastic agents and the reverse of drug resistant is essential for cancer therapy. Herein, we developed a drug delivery system which can simultaneously detect/reverse the drug resistance and perform synergetic treatment of cancer. In this work, we integrated cyanine5（Cy5） modified mi RNA（let-7 i）（Cy5-mi RNA） and platinum onto nano-graphene oxide（NGO）（30-50 nm） platform to achieve simultaneously detection/reversion of drug resistance and synergetic treatment of cisplatin resistant SKOV3 cells（SKOV3 DDP cells）. The Cy5-mi RNA adsorbed on NGO could selectively bind the drug resistance related m RNA follow by suppress the expression of drug resistance m RNA, and the binding simultaneously induced the release of Cy5-mi RNA from the NGO, thus the fluorescence signal of Cy5 recovered and could be used for drug resistance monitoring.Moreover, the mi RNA suppressed the Cyclin D1 protein expressions thus reversed the drug resistance. The loaded platinum（Ⅳ）（Pt（Ⅳ）） was converted to the therapeutic platinum（Ⅱ）（Pt（Ⅱ）） in both tumor acidic and reductive environment responsive behavior. NGO furtherly performed photothermal therapy under near infrared（NIR） laser irradiation and enhanced the therapeutic effect. All in all, this nanoplatform realized detection/reversion of the drug resistance as well as synergetic chemical-photothermal treatment of ovarian cancer cells, which holds great promise in the treatment of drug resistant cancer cells.
