摘要:
Photodynamic therapy (PDT) provides apparent survival benefits for unresectable cholangiocarcinoma patients. the insufficient sensitivity of cancer cell to PDT treatment limits the clinical application. In this study, according to the GEO datasets, WNT7B expression was decreased by PDT treatment in cholangiocarcinoma samples. In cholangiocarcinoma cells, PDT treatment inhibited Wnt signaling, suppressed cell viability, and enhanced cell apoptosis. Within cholangiocarcinoma cells, PDT treatment induced p53 and miR-34a-5p expression. Under PDT treatment, p53 knockdown downregulated miR-34a-5p expression, whereas the inhibition effect of p53 knockdown on miR-34a-5p could be partially attenuated by agomir-34a-5p. p53 knockdown enhanced cell viability and suppressed cell apoptosis, whereas miR-34a-5p overexpression exerted opposite effects; miR-34a-5p overexpression partially attenuated p53 knockdown effects on PDT-treated cholangiocarcinoma cells. miR-34a-5p directly targeted WNT7B and inhibited WNT7B expression. Under PDT treatment, WNT7B knockdown inhibited the Wnt signaling and cell viability, and promoted cell apoptosis, while miR-34a-5p suppression showed the opposite trends; WNT7B knockdown partially attenuated miR-34a-5p inhibition effects on PDT-treated cholangiocarcinoma cells. In conclusion, PDT treatment induces p53-induced miR-34a transactivation to inhibit cholangiocarcinoma cell proliferation; the miR-34a-5p/WNT7B axis and Wnt signaling are involved. (C) 2021 Elsevier Inc. All rights reserved.
摘要:
Journal of Empirical Research on Human Research Ethics, Volume 17, Issue 3, Page 362-372, July 2022. <br/>This study aims to investigate the knowledge and attitudes of participants and potential participants in clinical trials toward electronic informed consent. We conducted a survey-based cross-sectional study in Hunan Province, China in March 2021. A total of 547 respondents were included in this study. All questions in an 8-item survey section assessing participants’ knowledge of electronic informed consent received correct answers from at least 70% of participants. In terms of attitude scores, most participants (86.3%) believed that electronic informed consent is more convenient than the paper-based version, and more than half (51.2%) believed that electronic informed consent could completely replace the paper-based version. Responses indicated that common concerns about electronic informed consent were its security and confidentiality, legal benefits, and implications for rights protection.
摘要:
Six flavonoids, namely, three undescribed biflavonoids, one undescribed 8-aryl flavonoid, and two known compounds, were isolated from Selaginella tamariscina (P.Beauv.) Spring. The structures and absolute configurations of those undescribed compounds were established by NMR spectroscopy data, HRESIMS analyses and electronic circular dichroism (ECD) analyses. In addition, all the isolates were evaluated for their hypoglycemic activity in HepG2 cells. Involvenflavone H, I, and J significantly increased glucose consumption in both normal and insulin-resistant HepG2 cells. Interestingly, these three compounds can effectively upregulate the protein expression of glucokinase (GCK) and adenylate cyclases (ADCYs). These results suggested that involvenflavone H, I, and J (especially involvenflavone J) may have potent hypoglycemic activity, which also provided promising molecular targets for the treatment of diabetes.
摘要:
Diabetes mellitus increases the risk of dementia, and evidence suggests hyperglycemia is a key contributor to neurodegeneration. However, our understanding of diabetes-associated cognitive decline, an important complication of diabetes mellitus, is lacking and the underlying mechanism is unclear. Blood brain barrier (BBB) breakdown is a possible cause of dementia in diabetes mellitus and Alzheimer's disease. Accumulating evidence shows BBB dysfunction caused by hyperglycemia contributes to cognitive decline. A specific type of inflammatory programmed cell death, called pyroptosis, has potential as a therapeutic target for BBB-associated diseases. Potential inducers of pyroptosis include inflammasomes such as NLRP3, whose activation relies on damage-associated molecular patterns. High mobility group box 1 (HMGB1) is a highly conserved, ubiquitous protein found in most cell types, and acts as a damage-associated molecular pattern when released from the nucleus. We propose that HMGB1 influences vascular inflammation by activating the NLRP3 inflammasome and thereby initiating pyroptosis in vascular cells. Moreover, HMGB1 plays a pivotal role in the pathogenesis of diabetes mellitus and diabetic complications. Here, we review the role of HMGB1 in BBB dysfunction induced by hyperglycemia and propose that HMGB1 is a promising therapeutic target for countering diabetes-associated cognitive decline.
作者机构:
[Jiao, Liqun; Xu, Ran; Yang, Bin; Wang, Tao; Luo, Jichang; Ma, Yan] China Int Neurosci Inst China INI, Beijing, Peoples R China.;[Jiao, Liqun; Xu, Ran; Yang, Bin; Wang, Tao; Luo, Jichang; Ma, Yan] Capital Med Univ, Dept Neurosurg, Xuanwu Hosp, Beijing, Peoples R China.;[Liao, Wanying] Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, Beijing, Peoples R China.;[Wang, Xue] Capital Med Univ, Xuanwu Hosp, Med Lib, Beijing, Peoples R China.;[Li, Wei] Liaocheng Peoples Hosp, Dept Neurosurg, Liaocheng, Shandong, Peoples R China.
通讯机构:
[Yang, B.; Jiao, L.] C;China International Neuroscience Institute (China-INI)China
关键词:
CLINICAL PHARMACOLOGY;Coronary heart disease;Lipid disorders;Protocols & guidelines;VASCULAR MEDICINE
期刊:
Frontiers in Cellular and Infection Microbiology,2022年12:1004845 ISSN:2235-2988
作者机构:
[Xiao, Nenqun; Zhou, Kang; Peng, Maijiao; Yi, Xin] Hunan Univ Chinese Med, Coll Pharm, Changsha, Peoples R China.;[Cai, Ying; Deng, Na] Hunan Univ Chinese Med, Coll Chinese Med, Changsha, Peoples R China.
关键词:
Baohe pill decoction;lactase-producing bacteria;high-fat and high-protein diet;Diarrhea;intestinal content
摘要:
Background: This study investigated the effects of Baohe pill decoction on the diversity and community composition of lactase-producing bacteria in the intestinal contents of mice with diarrhea induced by high-fat and high-protein diet, which provided an experimental basis for the study on the therapeutic mechanism of Baohe pill decoction. Materials and methods: The Traditional Chinese Medicine Systems Pharmacology (TCMSP), DisGeNET, UniProt, National Center for Biotechnology Information (NCBI), and GeneCards databases were used to collect the potential targets with active ingredients of Baohe pill decoction, diarrhea, and lactase, and then construct correlation networks. Fifteen Kunming mice were randomly divided into the control group (CN), natural recovery group (NR), and Baohe pill decoction treatment group (BHP), with five mice in each group. After constructing a mouse diarrhea model by HFHPD induction, BHP was gavaged with Baohe pill decoction, and the other groups were gavaged with distilled water of equal. The intestinal contents were collected from ileal to jejunal and analyzed using metagenomic sequencing to characterize the intestinal content of lactase-producing bacteria in mice. Results: The core active ingredients related to diarrhea in Baohe pill decoction were quercetin, luteolin, kaempferol, forsythin, and wogonin. And there was no intersection between the potential targets with the active ingredient of Baohe pill, lactase, and diarrhea. After the intervention of Baohe pill decoction, the Observed species, Chao1 index, and Operational Taxonomic Units (OTU) number increased in BHP (P > 0.05), while the Pielous evenness and Shannon index decreased (P > 0.05). In Beta diversity, the community structure of the NR was significantly different from CN and BHP (P < 0.05), and the community structure of the CN was not significant difference from BHP (P > 0.05). Compared to NR, the relative abundance of Bifidobacterium and Amycolatopsis increased, while the relative abundance of Lachnoclostridium, Sinorhizobium, Cedecea, and Escherichia decreased in BHP, but none of the significant differences (P > 0.05). Conclusion: The therapeutic effect of Baohe pill decoction on diarrhea induced by HFHPD does not appear to involve the body's lactase gene targets directly, but is associated with the change of the construction of lactase-producing bacterial communities.
期刊:
Eating and Weight Disorders,2022年27(8):3187-3196 ISSN:1124-4909
通讯作者:
Zhang, Bin;He, Jinbo
作者机构:
[Tang, Chanyuan; He, Jinbo] Chinese Univ Hong Kong, Sch Humanities & Social Sci, Shenzhen 518172, Guangdong, Peoples R China.;[Barnhart, Wesley R.] Bowling Green State Univ, Dept Psychol, Bowling Green, OH 43403 USA.;[Zhang, Bin] Hunan Univ Chinese Med, Dept Appl Psychol, Changsha 410208, Hunan, Peoples R China.
通讯机构:
[Zhang, Bin] T;[He, Jinbo] S;The Department of Applied Psychology, Hunan University of Chinese Medicine, Changsha, 410208, Hunan, China.;School of Humanities and Social Science, The Chinese University of Hong Kong, Shenzhen, 518172, Guangdong, China.
关键词:
Validation;Psychometric properties;Drive for muscularity;Body image;Chinese
摘要:
Metabolomics is applied to explore the curative effect of complex systems, such as Chinese medicine. Intrauterine adhesion (IUA) harms the reproductive system and affects fertility, and hence is a significant public health concern. Prunella vulgaris oil (PVO) protects the reproductive system and exerts anti-inflammatory effects, but its effect on IUA and the underlying mechanism is unclear. In this study, we established a serum metabolomics method based on GC-TOF-MS to evaluate the mechanism of PVO in the IUA rat model established by mechanical injury and infection. Animal experiments showed that PVO improves the inflammatory response in the uterus of IUA model rats and reduces the content of inflammatory factors to improve the microenvironment of the reproductive system. It also regulates the expression of TGF-beta 1 and Smad-related mRNA and protein to inhibit fibrosis. Metabolomics indicated a significant abnormality in serum metabolism in IUA rats, and a total of 51 differential markers were screened and identified. After PVO treatment, these metabolic abnormalities improved significantly. The metabolic pathway analysis revealed that PVO affects glyoxylate and dicarboxylate metabolism, and beta-alanine metabolism pathways. This study showed that PVO significantly improves inflammation and fibrosis in IUA rats combined with the pharmacological results. The primary mechanism is related to regulating the metabolism of amino acids and their derivatives to balance the associated disorders and control energy metabolism. (c) 2021 Elsevier B.V. All rights reserved.
期刊:
Cellular and Molecular Neurobiology,2022年43(5):1931-1940 ISSN:0272-4340
通讯作者:
Yuhong Wang
作者机构:
[Jiang, Yajie; Ren, Tingting; Wang, Yuhong; Zou, Manshu] Hunan Univ Chinese Med, Inst Innovat & Appl Res Chinese Med, Changsha, Peoples R China.;[Jiang, Yajie; Ren, Tingting; Wang, Yuhong; Zou, Manshu] Hunan Univ Chinese Med, Hunan Key Lab Tradit Chinese Med Prevent & Treatm, Changsha, Peoples R China.
通讯机构:
[Yuhong Wang] I;Institute of Innovation and Applied Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China<&wdkj&>Hunan Key Laboratory of Traditional Chinese Medicine Prevention & Treatment of Depressive Diseases, Hunan University of Chinese Medicine, Changsha, China
摘要:
Depression is the most common mental illness characterized by anhedonia, avolition and loss of appetite and motivation. The majority of conventional antidepressants are monoaminergic system selective inhibitors, yet the efficacies are not sufficient. Up to 30% of depressed patients are resistant to treatment with available antidepressants, underscoring the urgent need for development of novel therapeutics to meet clinical needs. Recent years, compounds acting on the glutamate system have attracted wide attention because of their strong, rapid and sustained antidepressant effects. Among them, selective inhibitors of metabotropic glutamate receptors 2 and 3 (mGluR2/3) have shown robust antidepressant benefits with fewer side-effects in both preclinical and clinical studies. Thus, we here attempt to summarize the antidepressant effects and underlying mechanisms of these inhibitors revealed in recent years as well as analyze the potential value of mGluR2/3 selective inhibitors in the treatment of depression.
期刊:
Emergency Medicine International,2022年2022 ISSN:2090-2840
作者机构:
[He, Lianyi] First Hosp Hunan Univ Chinese Med, Dept Cadre Hlth Care, Changsha 410007, Hunan, Peoples R China.;[Chen, Xinyu] First Hosp Hunan Univ Chinese Med, Dept Cardiovasc Med, Changsha 410007, Hunan, Peoples R China.;[Zhang, Yuejuan] Hosp Hunan Univ Chinese Med, Changsha 410007, Hunan, Peoples R China.
摘要:
<i>Objective</i>. To explore the clinical effect of Hufu copper scraping on shoulder-hand syndrome after stroke. <i>Methods.</i> A total of 60 patients with shoulder-hand syndrome after stroke admitted to the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine were enrolled between January 2020 and June 2021. According to the random number table method, they were divided into the control group (<i>n</i> = 30) and the intervention group (<i>n</i> = 30). The control group was given routine rehabilitation intervention, while the intervention group was additionally given Hufu copper scraping. The intervention effect, occurrence of adverse reactions during the intervention, pain, swelling degree of affected hands, serum calcitonin gene-related peptide (CGRP), substance P (SP), and Barthel index before and after 2 months of intervention were compared between the two groups. <i>Results.</i> After intervention, the total response rate of the intervention group was significantly higher than that of the control group (96.67% <i>vs</i> 80.00%) (<span class="inline_break"><svg xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" width="19.289pt" style="vertical-align:-0.6370001pt" id="M1" height="9.2729pt" version="1.1" viewBox="-0.0498162 -8.6359 19.289 9.2729"><g transform="matrix(.013,0,0,-0.013,0,0)"><path id="g113-81" d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 195 42 212 127L243 284L314 263C327 263 339 263 352 264C465 271 600 337 600 480ZM508 481C508 351 402 304 329 304C289 304 265 311 250 317L295 559C302 594 310 606 323 611C335 616 350 619 367 619C455 619 508 573 508 481Z"/></g><g transform="matrix(.013,0,0,-0.013,11.658,0)"><path id="g117-91" d="M512 -3V55L134 254V256L512 456V514L75 281V230L512 -3Z"/></g></svg><span class="irelop"/><span class="nowrap"><svg xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" width="21.918pt" style="vertical-align:-0.6370001pt" height="9.2729pt" version="1.1" viewBox="22.8711838 -8.6359 21.918 9.2729"><g transform="matrix(.013,0,0,-0.013,22.921,0)"><path id="g113-49" d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"/></g><g transform="matrix(.013,0,0,-0.013,29.161,0)"><path id="g113-47" d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z"/></g><g transform="matrix(.013,0,0,-0.013,32.125,0)"><use xlink:href="#g113-49"/></g><g transform="matrix(.013,0,0,-0.013,38.365,0)"><path id="g113-54" d="M153 550H386L412 615L406 623H120L82 318C104 327 142 338 184 338C294 338 347 275 347 187C347 112 305 39 221 39C160 39 119 71 97 89C88 97 80 96 71 90C59 80 50 67 49 57C48 45 52 36 66 23C80 9 123 -12 169 -12C221 -11 288 15 342 59C403 109 431 165 431 225C431 308 366 395 238 395C212 395 165 379 127 364L153 550Z"/></g></svg>).</span></span> After intervention, the score of the visual analogue scale (VAS) and water displacement in the intervention group were lower than those in the control group (<span class="inline_break"><svg xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" width="19.289pt" style="vertical-align:-0.6370001pt" id="M2" height="9.2729pt" version="1.1" viewBox="-0.0498162 -8.6359 19.289 9.2729"><g transform="matrix(.013,0,0,-0.013,0,0)"><use xlink:href="#g113-81"/></g><g transform="matrix(.013,0,0,-0.013,11.658,0)"><use xlink:href="#g117-91"/></g></svg><span class="irelop"/><span class="nowrap"><svg xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" width="21.918pt" style="vertical-align:-0.6370001pt" height="9.2729pt" version="1.1" viewBox="22.8711838 -8.6359 21.918 9.2729"><g transform="matrix(.013,0,0,-0.013,22.921,0)"><use xlink:href="#g113-49"/></g><g transform="matrix(.013,0,0,-0.013,29.161,0)"><use xlink:href="#g113-47"/></g><g transform="matrix(.013,0,0,-0.013,32.125,0)"><use xlink:href="#g113-49"/></g><g transform="matrix(.013,0,0,-0.013,38.365,0)"><use xlink:href="#g113-54"/></g></svg>).</span></span> After intervention, levels of CGRP and SP in the intervention group were lower than those in the control group, while the Barthel index was higher compared to the control group (<span class="inline_break"><svg xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" width="19.289pt" style="vertical-align:-0.6370001pt" id="M3" height="9.2729pt" version="1.1" viewBox="-0.0498162 -8.6359 19.289 9.2729"><g transform="matrix(.013,0,0,-0.013,0,0)"><use xlink:href="#g113-81"/></g><g transform="matrix(.013,0,0,-0.013,11.658,0)"><use xlink:href="#g117-91"/></g></svg><span class="irelop"/><span class="nowrap"><svg xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" width="21.918pt" style="vertical-align:-0.6370001pt" height="9.2729pt" version="1.1" viewBox="22.8711838 -8.6359 21.918 9.2729"><g transform="matrix(.013,0,0,-0.013,22.921,0)"><use xlink:href="#g113-49"/></g><g transform="matrix(.013,0,0,-0.013,29.161,0)"><use xlink:href="#g113-47"/></g><g transform="matrix(.013,0,0,-0.013,32.125,0)"><use xlink:href="#g113-49"/></g><g transform="matrix(.013,0,0,-0.013,38.365,0)"><use xlink:href="#g113-54"/></g></svg>).</span></span> <i>Conclusion.</i> The intervention effect of Hufu copper scraping is good, which can promote the recovery of related symptoms, relieve pain and swelling of affected hands, downregulate levels of disease-promoting markers, and accelerate recovery of related function in patients with shoulder-hand syndrome after stroke.
期刊:
Frontiers in Pharmacology,2022年13:960140 ISSN:1663-9812
作者机构:
[Li, Kai; Fu, Rong-geng; Lin, Xiu-lian; Cao, Yu-ling; Luo, Shi-teng; Xia, Bo-hou; Tan, Xi; Liao, Duan-fang; Lin, Li-mei; Shi, Ya-ning; Li, Ya-mei; Cao, Deliang; Zeng, Ya-ling] Hunan Univ Chinese Med, Key Lab Qual Evaluat Bulk Herbs Hunan Prov, Changsha, Hunan, Peoples R China.;[Qin, Li; Liao, Duan-fang; Shi, Ya-ning] Hunan Univ Chinese Med, Div Stem Cell Regulat & Applicat, Changsha, Hunan, Peoples R China.;[Zeng, Jian-guo] Hunan Agr Univ, Hunan Key Lab Tradit Chinese Vet Med, Changsha, Hunan, Peoples R China.
关键词:
sanguinarine;small intestine injury;indomethacin;Nrf2/NF-kappa B pathways;rats;IEC-6 cells
摘要:
In recent years, small intestine as a key target in the treatment of Inflammatory bowel disease caused by NSAIDs has become a hot topic. Sanguinarine (SA) is one of the main alkaloids in the Macleaya cordata extracts with strong pharmacological activity of anti-tumor, anti-inflammation and anti-oxidant. SA is reported to inhibit acetic acid-induced colitis, but it is unknown whether SA can relieve NSAIDs-induced small intestinal inflammation. Herein, we report that SA effectively reversed the inflammatory lesions induced by indomethacin (Indo) in rat small intestine and IEC-6 cells in culture. Our results showed that SA significantly relieved the symptoms and reversed the inflammatory lesions of Indo as shown in alleviation of inflammation and improvement of colon macroscopic damage index (CMDI) and tissue damage index (TDI) scores. SA decreased the levels of TNF-alpha, IL-6, IL-1 beta, MDA and LDH in small intestinal tissues and IEC-6 cells, but increased SOD activity and ZO-1 expression. Mechanistically, SA dose-dependently promoted the expression of Nrf2 and HO-1 by decreasing Keap-1 level, but inhibited p65 phosphorylation and nuclear translocation in Indo-treated rat small intestine and IEC-6 cells. Furthermore, in SA treated cells, the colocalization between p-p65 and CBP in the nucleus was decreased, while the colocalization between Nrf2 and CBP was increased, leading to the movement of gene expression in the nucleus to the direction of anti-inflammation and anti-oxidation. Nrf2 silencing blocked the effects of SA. Together our results suggest that SA can significantly prevent intestinal inflammatory lesions induced by Indo in rats and IEC-6 cells through regulation of the Nrf2 pathway and NF-kappa Bp65 pathway.
摘要:
BackgroundScholars have long understood that gastrointestinal microorganisms are intimately related to human disorders. The literature on research involving the gut microbiome and neuroscience is emerging. This study exposed the connections between gut microbiota and neuroscience methodically and intuitively using bibliometrics and visualization. This study's objectives were to summarize the knowledge structure and identify emerging trends and potential hotspots in this field. Materials and methodsOn October 18, 2022, a literature search was conducted utilizing the Web of Science Core Collection (WoSCC) database for studies on gut microbiota and neuroscience studies from 2002 to 2022 (August 20, 2022). VOSviewer and CiteSpace V software was used to conduct the bibliometrics and visualization analysis. ResultsFrom 2002 to 2022 (August 20, 2022), 2,275 publications in the WoSCC database satisfied the criteria. The annual volume of publications has rapidly emerged in recent years (2016-2022). The most productive nation (n = 732, 32.18%) and the hub of inter-country cooperation (links: 38) were the United States. University College Cork had the most research papers published in this area, followed by McMaster University and Harvard Medical School. Cryan JF, Dinan TG, and Clarke G were key researchers with considerable academic influence. The journals with the most publications are "Neurogastroenterology and Motility" and "Brain Behavior and Immunity." The most cited article and co-cited reference was Cryan JF's 2012 article on the impact of gut microbiota on the brain and behavior. The current research hotspot includes gastrointestinal microbiome, inflammation, gut-brain axis, Parkinson's disease (PD), and Alzheimer's disease (AD). The research focus would be on the "gastrointestinal microbiome, inflammation: a link between obesity, insulin resistance, and cognition" and "the role of two important theories of the gut-brain axis and microbial-gut-brain axis in diseases." Burst detection analysis showed that schizophrenia, pathology, and psychiatric disorder may continue to be the research frontiers. ConclusionResearch on "gastrointestinal microbiome, inflammation: a link between obesity, insulin resistance, and cognition" and "the role of two important theories of the gut-brain axis and microbial-gut-brain axis in diseases" will continue to be the hotspot. Schizophrenia and psychiatric disorder will be the key research diseases in the field of gut microbiota and neuroscience, and pathology is the key research content, which is worthy of scholars' attention.
期刊:
Emergency Medicine International,2022年2022 ISSN:2090-2840
作者机构:
[Liu, Caidan; Luo, Huanwen] Hunan Univ Chinese Med, Dept Neurol, Hosp 1, Changsha 410000, Hunan, Peoples R China.;[Luo, Hong; Wang, Zhongying] Grad Sch Hunan Univ Chinese Med, Changsha 410000, Hunan, Peoples R China.;[Yu, Yanlan] First Hosp Hunan Univ Chinese Med, Dept Nursing, Changsha 410000, Hunan, Peoples R China.
摘要:
<i>Objective</i>. The aim of this study is to investigate the efficacy of moxibustion combined with an ear acupoint pressing bean in the treatment of patients with phlegm stasis syndrome vertigo. <i>Methods</i>. 60 patients with vertigo identified as phlegm stasis syndrome who were hospitalized in our department from May 2020 to May 2021 were selected and divided into a control group and a treatment group of 30 cases each according to the random number method. The control group was treated with conventional treatment and care, and the treatment group was treated with moxibustion combined with ear acupressing beans on top of the conventional group. The treatment effects, the dizziness disorder inventory (DHI), Pittsburgh sleep quality index (PSQI), Hamilton anxiety score (HAMA), TCM symptom score, and blood flow parameters (left vertebral artery flow velocity (LVA), right vertebral artery flow velocity (RVA), and basilar artery flow velocity (BA)) of the two groups were compared with each other during and after the treatment. <i>Results</i>. After implementation, the treatment efficiency of the treatment group was higher than that of the control group, and the treatment group had lower PSQI, HAMA, and DHI scores as well as TCM symptom scores such as vertigo, head heavy as a wrap, chest tightness, and nausea and vomiting than the control group (<span class="inline_break"><svg xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" width="19.289pt" style="vertical-align:-0.6370001pt" id="M1" height="9.2729pt" version="1.1" viewBox="-0.0498162 -8.6359 19.289 9.2729"><g transform="matrix(.013,0,0,-0.013,0,0)"><path id="g113-81" d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 195 42 212 127L243 284L314 263C327 263 339 263 352 264C465 271 600 337 600 480ZM508 481C508 351 402 304 329 304C289 304 265 311 250 317L295 559C302 594 310 606 323 611C335 616 350 619 367 619C455 619 508 573 508 481Z"/></g><g transform="matrix(.013,0,0,-0.013,11.658,0)"><path id="g117-91" d="M512 -3V55L134 254V256L512 456V514L75 281V230L512 -3Z"/></g></svg><span class="irelop"/><span class="nowrap"><svg xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" width="21.918pt" style="vertical-align:-0.6370001pt" height="9.2729pt" version="1.1" viewBox="22.8711838 -8.6359 21.918 9.2729"><g transform="matrix(.013,0,0,-0.013,22.921,0)"><path id="g113-49" d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"/></g><g transform="matrix(.013,0,0,-0.013,29.161,0)"><path id="g113-47" d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z"/></g><g transform="matrix(.013,0,0,-0.013,32.125,0)"><use xlink:href="#g113-49"/></g><g transform="matrix(.013,0,0,-0.013,38.365,0)"><path id="g113-54" d="M153 550H386L412 615L406 623H120L82 318C104 327 142 338 184 338C294 338 347 275 347 187C347 112 305 39 221 39C160 39 119 71 97 89C88 97 80 96 71 90C59 80 50 67 49 57C48 45 52 36 66 23C80 9 123 -12 169 -12C221 -11 288 15 342 59C403 109 431 165 431 225C431 308 366 395 238 395C212 395 165 379 127 364L153 550Z"/></g></svg>).</span></span> In addition, LVA, RVA, and BA were all higher in the treatment group than in the control group after treatment (<span class="inline_break"><svg xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" width="19.289pt" style="vertical-align:-0.6370001pt" id="M2" height="9.2729pt" version="1.1" viewBox="-0.0498162 -8.6359 19.289 9.2729"><g transform="matrix(.013,0,0,-0.013,0,0)"><use xlink:href="#g113-81"/></g><g transform="matrix(.013,0,0,-0.013,11.658,0)"><use xlink:href="#g117-91"/></g></svg><span class="irelop"/><span class="nowrap"><svg xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" width="21.918pt" style="vertical-align:-0.6370001pt" height="9.2729pt" version="1.1" viewBox="22.8711838 -8.6359 21.918 9.2729"><g transform="matrix(.013,0,0,-0.013,22.921,0)"><use xlink:href="#g113-49"/></g><g transform="matrix(.013,0,0,-0.013,29.161,0)"><use xlink:href="#g113-47"/></g><g transform="matrix(.013,0,0,-0.013,32.125,0)"><use xlink:href="#g113-49"/></g><g transform="matrix(.013,0,0,-0.013,38.365,0)"><use xlink:href="#g113-54"/></g></svg>).</span></span> <i>Conclusion</i>. Moxibustion combined with ear acupoint pressing bean treatment can clearly improve patients’ sleep quality, psychological state, relieve patients’ various symptoms caused by vertigo, improve blood flow parameters, and have better efficacy in the treatment of phlegm stasis syndrome vertigo.
作者机构:
[Wang, Xiaoqi; Ning, Yi; Lu, Fangguo; Hu, Jue; Xiao, Rong; Liu, Shiwu] Hunan Univ Chinese Med, Dept Microbiol, Med Sch, Changsha 410208, Hunan, Peoples R China.;[Li, Ling] Hunan Univ Chinese Med, Expt Ctr Mol Biol, Med Sch, Changsha 410208, Hunan, Peoples R China.
通讯机构:
[Yi Ning; Fangguo Lu] D;Department of Microbiology, The Medicine School of Hunan University of Chinese Medicine, Changsha, Hunan, 410208, People's Republic of China<&wdkj&>Department of Microbiology, The Medicine School of Hunan University of Chinese Medicine, Changsha, Hunan, 410208, People's Republic of China
期刊:
Microbes and Infection,2022年24(8):104998 ISSN:1286-4579
通讯作者:
Ling, Li
作者机构:
[Huang, Jiawang; Ma, Xinyue; Zhu, Mengchen; Chen, Yulu] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Xueshi Rd 300, Changsha 410208, Hunan, Peoples R China.;[Lu, Fang-guo; Zhang, Bo; Hu, Jue; Cheng, Lijuan] Hunan Univ Chinese Med, Dept Microbiol, Med Sch, 300 Rd, Changsha, Hunan, Peoples R China.;[Li, Ling; Liu, Weirong] Hunan Univ Chinese Med, Coll Tradit Chinese Med, 300 Rd, Changsha, Hunan, Peoples R China.
通讯机构:
[Ling, Li] T;The College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, 300 Road, Yuelu District, Changsha, Hunan Province, China. Electronic address:
摘要:
Acute lung injury (ALI) is characterized by tissue damage that leads to pulmonary epithelial membrane dysfunction and macrophage activation. Currently however, the exact mechanism by which the initial mediators of mouse lung epithelial (MLE-12) cells induce inflammation remines unclear. We constructed co-culture systems of MLE-12 cells with mouse macrophage cells RAW246.7 which were realized by the supernatant and Transwell chamber. In previous study, we successfully constructed an influenza A virus-induced MLE-12 cells model. Extracellular Vesicles (EVs) from cells supernatant were isolated by differential ultracentrifugation and confirmed by transmission electron microscopy. High-throughput sequencing results showed that MLE-12 cells stimulated by influenza A virus had higher level of miR-1249-5p. The results were validated by RT-qPCR analysis. The research aimed to investigate the roles and mechanisms of miR-1249-5p in ALI. RAW246.7 cells were transfected with miR-1249-5p mimic/inhibitor. The concentrations of TNF-alpha, IL-6 were determined by ELISA and the uptake of EVs was monitored by confocal laser scanning microscope. Western blotting detected changes in the SLC4A1 and NF-kappa B signaling pathway. The results indicated that miR-1249-5p played an important role in ALI, and further investigation of its target gene SLC4A1 and NF-kappa B signaling pathway provides ideas for new therapeutic targets and strategies for ALI. (C) 2022 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
作者机构:
[Chen, Kaiqin; Wang, Xiaoqi; Ning, Yi; Lu, Fangguo; Xiong, Tao; Hu, Jue; Wei, Ke; Xiao, Rong] Hunan Univ Chinese Med, Sch Med, Changsha 410128, Peoples R China.;[Chen, Shanquan] Chinese Univ Hong Kong, Sch Humanities & Social Sci, Shenzhen 518172, Peoples R China.;[Lu, Fangguo] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha 410021, Peoples R China.;[Lu, Fangguo] Hunan Univ Chinese Med, Med Coll, Changsha 410128, Peoples R China.
通讯机构:
[Shanquan Chen; Fangguo Lu] S;School of Humanities and Social Sciences, The Chinese University of Hong Kong, Shenzhen, 518172, China<&wdkj&>School of Medicine, Hunan University of Chinese Medicine, Changsha, 410128, China<&wdkj&>The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410021, China
摘要:
Xiaoqu is a saccharifying and alcohol-producing, flavoring agent used in brewing Chinese rice wine (CRW). We combined high-throughput sequencing with headspace solid-phase microextraction-mass spectrometry-gas phase ion migration spectrometry to identify the microbial communities of four Xiaoqu rice wines collected from Xinhua County, and the flavoring substances of CRWs brewed with them. The most prevalent microorganisms at the genus level were Pediococcus, Enterobacter, Leuconostoc, Weissella, Staphylococcus, Pantoea, Glutamicibacter, Kosakonia, Lactobacillus, Rhizopus, and Mucor. We detected 43 volatile flavoring esters, alcohols, aldehydes, acids, and ketones in the Xiaoqu CRWs. We identified significant positive correlations between Pediococcus/Weissella/ Lactobacillus and ethyl butyrate, Pantoea/Kosakonia and 3-methyl-1-butanol/2-methyl-1-propanol, Lactococcus/ Streptococcus and ethyl lactate, and Apibacter/Rosenbergiella and ethyl acetate/3-methyl-1-butanol. Relationships between Pediococcus and Lactobacillus/Streptococcus, Rhizopus and Weissella, Mucor, and Leuconostoc, Hydropichia and almost all dominant bacterial genera, as well as Rhizopus and five other fungal genera were synergistic. Carbohydrate, coenzyme, and amino acid metabolism might be the main metabolic processes of dominant bacteria in Xiaoqu. We clarified the dominant microbial community structures of Xinhua Xiaoqu, characterized the flavor compounds in CRW and the relationships between microbiota and flavor. Our findings provide reference data for future flavor-based screens of beneficial bacterial and fungal strains to improve the quality of Xiaoqu CRW.