期刊：
Frontiers in Bioscience-Landmark,2023年28(10):271 ISSN：2768-6701
通讯作者：
Yu, Lili;Wu, QB;Fan, XM
作者机构：
[Luo, Dan; Wang, Jue; Liang, Yuling; Yu, Lili; Wu, Qibiao] Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Fac Chinese Med, Taipa 999078, Macao, Peoples R China.;[Luo, Dan; Fan, Xianming; Wang, Wenjun; Gui, Xuemei; Yan, Jie; Fang, Mengying; Liang, Yuling; Chen, Mengqin] Southwest Med Univ, Affiliated Hosp, Dept Resp & Crit Care Med, Luzhou 646099, Sichuan, Peoples R China.;[Luo, Dan; Fan, Xianming; Wang, Wenjun; Gui, Xuemei; Yan, Jie; Fang, Mengying; Liang, Yuling; Chen, Mengqin] Southwest Med Univ, Affiliated Hosp, Inflammat & Allerg Dis Res Unit, Luzhou 646099, Sichuan, Peoples R China.;[Shao, Le] Hunan Univ Chinese Med, Hosp 1, Ctr Med Res & Innovat, Changsha 410021, Hunan, Peoples R China.;[Wu, Qibiao] Guangdong Univ Technol, Guangdong Hong Kong Macao Joint Lab Contaminants, Guangzhou 510520, Peoples R China.
通讯机构：
[Yu, LL; Wu, QB ] M;[Fan, XM ] S;Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Fac Chinese Med, Taipa 999078, Macao, Peoples R China.;Southwest Med Univ, Affiliated Hosp, Dept Resp & Crit Care Med, Luzhou 646099, Sichuan, Peoples R China.;Southwest Med Univ, Affiliated Hosp, Inflammat & Allerg Dis Res Unit, Luzhou 646099, Sichuan, Peoples R China.
摘要：
BACKGROUND: Lung cancer is the main cause of cancer-related death, with epithelial-mesenchymal transition (EMT) playing an important role in the development of this disease. The EMT-related genes Polypeptide N-Acetylgalactosaminyltransferase 3 (GALNT3) and 2'-5'-Oligoadenylate Synthetase 1 (OAS1) are involved in numerous tumor processes. Although these genes have been extensively studied in cancer, they have yet to be analyzed by multi-omics in lung adenocarcinoma (LUAD). METHODS: EMT-related genes were identified by R and Venn diagram. Cox regression and Kaplan-Meier analysis were performed to evaluate patient survival, and the Gene Expression Profiling Interactive Analysis (GEPIA) database was used for correlation analysis. GeneCards and R packages were used to explore gene characterization and functional annotation. The Tumor Immune Estimation Resource (TIMER), Human Protein Atlas (HPA), University of Alabama at Birmingham Cancer (UALCAN), and The Cancer Genome Atlas (TCGA) databases were used to investigate gene expression, which was then confirmed by RT-PCR. Clinicopathological analysis was carried out using the UALCAN database. Functional mechanisms and multi-omics analysis were performed using DNA Methylation Interactive Visualization Database (DNMIVD), Targetscan, TIMER, Tumor-immune System Interactions Database (TISIDB) and cBioportal. Diagnostic values were calculated using ROC curve analysis. RESULTS: A total of 320 EMT-related genes were identified in LUAD. Their characteristics were confirmed in the Database for Annotation, Visualization and Integrated Discovery (DAVID) database by the intersection of 855 and 3600 different genes from the Gene Expression Omnibus (GEO) and EMTome databases, respectively. Expression of the EMT-related genes GALNT3 and OAS1 was associated with the prognosis of LUAD patients. A positive correlation was observed between the expression of GALNT3 and OAS1, and their expression was higher in LUAD tissue than in normal lung tissue. This was confirmed using RT-PCR. Multi-omics analysis revealed that GALNT3 and OAS1 expression was associated with gene mutation and methylation, cellular immune infiltration, and several immune subtypes. A miRNA-GALNT3/OAS1 regulatory network was also found. Receiver operating characteristic (ROC) curve analysis found that GALNT3 and OAS1 expression combined had superior diagnostic value to that of each marker alone. CONCLUSIONS: GALNT3 and OAS1 expression are associated with immune cell infiltration and poor prognosis in LUAD. Their combined expression has high diagnostic value; hence, GALNT3 and OAS1 may be valuable biomarkers for the early detection of LUAD.
摘要：
Background: Among the 382 million diabetic patients worldwide, approximately 30% experience neuropathy, and one-fifth of these patients eventually develop diabetes cognitive impairment (CI). However, the mechanism underlying diabetes CI remains unknown, and early diagnostic methods or effective treatments are currently not available.Objective: This study aimed to explore the risk factors for CI in patients with type 2 diabetes mellitus (T2DM), screen potential therapeutic drugs for T2DM-CI, and provide evidence for preventing and treating T2DM-CI.Methods: This study focused on the T2DM population admitted to the First Affiliated Hospital of Hunan College of Traditional Chinese Medicine and the First Affiliated Hospital of Hunan University of Chinese Medicine. Sociodemographic data and clinical objective indicators of T2DM patients admitted from January 2018 to December 2022 were collected. Based on the Montreal Cognitive Assessment (MoCA) Scale scores, 719 patients were categorized into two groups, the T2DM-CI group with CI and the T2DM-N group with normal cognition. The survey content included demographic characteristics, laboratory serological indicators, complications, and medication information. Six machine learning algorithms were used to analyze the risk factors of T2DM-CI, and the Shapley method was used to enhance model interpretability. Furthermore, we developed a graph neural network (GNN) model to identify potential drugs associated with T2DM-CI.Results: Our results showed that the T2DM-CI risk prediction model based on Catboost exhibited superior performance with an area under the receiver operating characteristic curve (AUC) of 0.95 (specificity of 93.17% and sensitivity of 78.58%). Diabetes duration, age, education level, aspartate aminotransferase (AST), drinking, and intestinal flora were identified as risk factors for T2DM-CI. The top 10 potential drugs related to T2DM-CI, including Metformin, Liraglutide, and Lixisenatide, were selected by the GNN model. Some herbs, such as licorice and cuscutae semen, were also included. Finally, we discovered the mechanism of herbal medicine interventions in gut microbiota.Conclusion: The method based on Interpreting AI and GNN can identify the risk factors and potential drugs associated with T2DM-CI.
期刊：
Journal of Clinical Nursing,2023年32(11-12):2399-2409 ISSN：0962-1067
通讯作者：
Chengwei Fu PhD<&wdkj&>Juan Yuan MD
作者机构：
[Chen, Wenzhen; Yuan, Juan] Anhui Univ Chinese Med, Sch Nursing, 103 Meishan Rd, Hefei 230012, Anhui, Peoples R China.;[Fu, Chengwei] Guangzhou Univ Chinese Med, Clin Med Sch 2, 12 Airport Rd, Guangzhou 510080, Guangdong, Peoples R China.;[Wu, Boyu] Hunan Univ Chinese Med, Changsha, Peoples R China.;[Zhou, Hong] Wuhan 1 Hosp, Wuhan, Peoples R China.;[Chen, Erfei] Univ Sci & Technol China, Sch Software Engn, Hefei, Peoples R China.
通讯机构：
[Chengwei Fu PhD; Juan Yuan MD] T;The Second Clinical Medical School, Guangzhou University of Chinese Medicine Guangzhou, China<&wdkj&>The School of Nursing, Anhui University of Chinese Medicine, Hefei, China
作者机构：
[Tang, Siqi; Huang, Hao; Li, Xiaojun; Wei, Kaixin; Suo, Zongwu; Xu, Yi] Gannan Med Univ, Natl Engn Res Ctr Modernizat Tradit Chinese Med, Sch Pharm, Hakka Med Resources Branch, Ganzhou 341000, Peoples R China.;[Liu, Dongxu; Li, Chunying; Zhao, Lei] Gannan Med Univ, Coll Basic Med, Ganzhou 341000, Peoples R China.;[Liu, Xiangqian] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Peoples R China.
通讯机构：
[Xiaojun Li] N;[Dongxu Liu] C;National Engineering Research Center for Modernization of Traditional Chinese Medicine—Hakka Medical Resources Branch, School of Pharmacy, Gannan Medical University, Ganzhou 341000, China<&wdkj&>College of Basic Medicine, Gannan Medical University, Ganzhou 341000, China
摘要：
Abstract: The traditional herb Eleutherococcus henryi Oliv. is commonly used to treat inflammatory conditions including rheumatism, arthritis, and hepatitis, as well as mental fatigue and amnesia, according to traditional Chinese medicine (TCM) theory. Savinin is a natural lignan obtained from the roots of E. henryi. The present study was undertaken to determine whether savinin can relieve LPS-induced neuroinflammation and if so, what the mechanism is. Groups of male C57BL/6 mice were administered savinin (5, 10, 20 mg/kg) and DEX (10 mg/kg) by gavage once daily for a continuous 7 days. On the 5th day of continuous pre-administration, LPS (2.5 mg/kg) was injected into the lateral ventricles of the mice for modeling 48 h. We found that treatment with savinin decreased the levels of neuroinflammatory cytokines and histopathological alterations dramatically. Consequently, it improved the LPS-induced neuroinflammatory response in mice. Furthermore, savinin inhibited the up-regulated expression of related proteins in the activated MAPK/NF-κB and NLRP3 inflammasome signaling pathways caused by LPS. Docking studies demonstrated the binding of savinin to three receptors (MAPK, NF-κB and NLRP3) using a well-fitting mode. These findings suggest that savinin may suppress neuroinflammation induced by LPS in vivo via modulating MAPK/NF-κB and NLRP3 signaling pathways. Keywords: savinin; neuroinflammation; MAPK; NF-κB; NLRP3 inflammasome
摘要：
Trastuzumab (Tra), the first humanized monoclonal antibody that targets human epidermal growth factor receptor 2 (HER2), is commonly used alongside doxorubicin (Dox) as a combination therapy in HER2-positive breast cancer. Unfortunately, this leads to a more severe cardiotoxicity than Dox alone. NLRP3 inflammasome is known to be involved in Dox-induced cardiotoxicity and multiple cardiovascular diseases. However, whether the NLRP3 inflammasome contributes to the synergistic cardiotoxicity of Tra has not been elucidated. In this study, primary neonatal rat cardiomyocyte (PNRC), H9c2 cells and mice were treated with Dox (15mg/kg in mice or 1μM in cardiomyocyte) or Tra (15.75mg/kg in mice or 1μM in cardiomyocyte), or Dox combined Tra as cardiotoxicity models to investigate this question. Our results demonstrated that Tra significantly potentiated Dox-induced cardiomyocyte apoptosis and cardiac dysfunction. These were accompanied by the increased expressions of NLRP3 inflammasome components (NLRP3, ASC and cleaved caspase-1), the secretion of IL-β and the pronounced production of ROS. Inhibiting the activation of NLRP3 inflammasome by NLRP3 silencing significantly reduced cell apoptosis and ROS production in Dox combined Tra-treated PNRC. Compared with the wild type mice, the systolic dysfunction, myocardial hypertrophy, cardiomyocyte apoptosis and oxidative stress induced by Dox combined Tra were alleviated in NLRP3 gene knockout mice. Our data revealed that the co-activation of NLRP3 inflammasome by Tra promoted the inflammation, oxidative stress and cardiomyocytes apoptosis in Dox combined Tra-induced cardiotoxicity model both in vivo and in vitro. Our results suggest that NLRP3 inhibition is a promising cardioprotective strategy in Dox/Tra combination therapy.
期刊：
Geriatric Orthopaedic Surgery & Rehabilitation,2023年14:21514593231195237 ISSN：2151-4585
通讯作者：
Zhang, YH;Yang, SF
作者机构：
[Li, Haili; Zhou, Jin] Luohe Cent Hosp, Hemodialysis Ctr, Luohe, Henan, Peoples R China.;[Liang, Hao; Jiang, Haobo] Hunan Univ Chinese Med, Sch Chinese Med, Changsha, Peoples R China.;[Wang, Jingye; Yang, Shaofeng; Jiang, Haobo; Guo, Zehua; Chen, Guangxue] Hunan Univ Chinese Med, Hosp 1, Dept Spinal Surg, Changsha, Hunan, Peoples R China.;[Zhang, Yonghui] Luohe Cent Hosp, Dept Joint Surg, Luohe, Henan, Peoples R China.;[Zhang, Yonghui] Luohe Cent Hosp, Dept Joint Surg, 56 Renmin East Rd, Luohe 462000, Henan, Peoples R China.
通讯机构：
[Yang, SF ] H;[Zhang, YH ] L;Luohe Cent Hosp, Dept Joint Surg, 56 Renmin East Rd, Luohe 462000, Henan, Peoples R China.;Hunan Univ Chinese Med, Hosp 1, Dept Spinal Surg, Changsha 410007, Hunan, Peoples R China.
关键词：
geriatric medicine;meta-analysis;metabolic bone disorders;mind-body exercise;osteoporosis
摘要：
Geriatric Orthopaedic Surgery & Rehabilitation, Volume 14, Issue , January-December 2023. <br/>IntroductionOsteoporosis is a major cause of fractures and even life-threatening fractures in the elderly. Mind-body exercise is a beneficial intervention to improve flexibility, control body balance and reduce pain. We aimed to evaluate the effects of physical and mental exercise on osteoporosis in the elderly.MethodsRandomized controlled trials (RCTs) focusing on mind-body exercises for osteoporosis were included. Web of Science, PubMed, Science Direct, Medline, Cochrane Library, China National Knowledge Infrastructure (CNKI), and Wanfang were searched from inception to January 2023. Outcomes included bone mineral density (BMD), bone mineral content (BMC), body balance (BB), pain, indicators of bone metabolism (BMI), lower extremity function, fearing level, and quality of life (QOL). The quality of study reporting was rated by 2 reviewers independently, and Review Manager software (version 5.3) was used for meta-analysis.ResultsThirty-nine trials with 2325 participants were included. The pooled results showed that mind-body exercises have encouraging effect on elderly people with osteoporosis, especially in aspects of BMD, BMC, QOL, improving the function of lower extremity, reducing pain and fearing level. While, dance and eight-section brocade could not improve the quality of life,or dance and eight-section brocade have no effect on BMD.ConclusionsMind-body exercises may have potential efficacy for osteoporosis in the elderly. However, due to the poor methodological quality of the included trials, more clinical trials with precise methodological design and rigorous reporting are needed.
摘要：
Heterotopic ossification (HO), including hereditary and acquired HO, is the formation of extraskeletal bone in skeletal muscle and surrounding soft tissues. Acquired HO is often caused by range of motion, explosion injury, nerve injury or burns. Severe HO can lead to pain and limited joint activity, affecting functional rehabilitation and quality of life. Increasing evidence shows that inflammatory processes and mesenchymal stem cells (MSCs) can drive HO. However, explicit knowledge about the specific mechanisms that result in HO and related cell precursors is still limited. Moreover, there are no effective methods to prevent or reduce HO formation. In this review, we provide an update of known risk factors and relevant cellular origins for HO. In particular, we focus on the underlying mechanisms of MSCs in acquired HO, which follow the osteogenic program. We also discuss the latest therapeutic value and implications for acquired HO. Our review highlights the current gaps in knowledge regarding the pathogenesis of acquired HO and identifies potential targets for the prevention and treatment of HO.
摘要：
BACKGROUND: Doxorubicin (Dox), a chemotherapeutic agent known for its efficacy, has been associated with the development of severe cardiotoxicity, commonly referred to as doxorubicin-induced cardiotoxicity (DIC). The role and mechanism of action of phloretin (Phl) in cardiovascular diseases are well-established; however, its specific function and underlying mechanism in the context of DIC have yet to be fully elucidated. OBJECTIVE: This research aimed to uncover the protective effect of Phl against DIC in vivo and in vitro, while also providing a comprehensive understanding of the underlying mechanisms involved. METHODS: DIC cell and murine models were established. The action targets and mechanism of Phl against DIC were comprehensively examined by systematic network pharmacology, molecular docking, transcriptomics technologies, transcription factor (TF) prediction, and experimental validation. RESULTS: Phl relieved Dox-induced cell apoptosis in vitro and in vivo. Through network pharmacology analysis, a total of 554 co-targeted genes of Phl and Dox were identified. Enrichment analysis revealed several key pathways including the PI3K-Akt signaling pathway, Apoptosis, and the IL-17 signaling pathway. Protein-protein interaction (PPI) analysis identified 24 core co-targeted genes, such as Fos, Jun, Hif1a, which were predicted to bind well to Phl based on molecular docking. Transcriptomics analysis was performed to identify the top 20 differentially expressed genes (DEGs), and 202 transcription factors (TFs) were predicted for these DEGs. Among these TFs, 10 TFs (Fos, Jun, Hif1a, etc.) are also the co-targeted genes, and 3 TFs (Fos, Jun, Hif1a) are also the core co-targeted genes. Further experiments validated the finding that Phl reduced the elevated levels of Hif3a (one of the top 20 DEGs) and Fos (one of Hif3a's predicted TFs) induced by Dox. Moreover, the interaction between Fos protein and the Hif3a promoter was confirmed through luciferase reporter assays. CONCLUSION: Phl actively targeted and down-regulated the Fos protein to inhibit its binding to the promoter region of Hif3a, thereby providing protection against DIC.
摘要：
Syndecan-1 (SDC-1) was a critical membrane proteoglycan and an important component of the glycocalyx in endothelial cells, but its role in atherosclerosis remains unknown. This study attempted to investigate the role of SDC-1 in atherosclerotic-related endothelial cell injury. Bioinformatics analyzed the differential miRNAs between atherosclerosis and healthy. Subjects with coronary atherosclerosis, which were diagnosed with intravascular atherosclerosis (IVUS), were enrolled as non-vulnerable plaque and vulnerable plaque in Changsha Central Hospital. Human aortic endothelial cells (HAECs) were induced by oxidized low-density lipoprotein (ox-LDL) to construct an in vitro model. A dual luciferase reporter assay was applied to analyze the target between miR-19a-3p and SDC-1. The cell proliferation and apoptosis were detected by CCK8 and flow cytometry, respectively. SDC-1 and cholesterol efflux was determined by ELISA. The expression of ATPbinding cassette (ABC) transports A1 (ABCA1), miR-19a-3p, ABCG1 and SDC-1 genes were detected by RT-qPCR. The expressions of SDC-1, ABCA1, ABCG1, TGF-& beta;1, Smad3 and p-Smad3 proteins were detected by western blot. Our results found that miR-19a-3p was down-regulated in atherosclerosis. ox-LDL decreased miR-19a-3p expression, increased cholesterol efflux and the expression of ABCA1, ABCG1 and SDC-1 in HAECs. Vulnerable plaque tissues in patients with coronary atherosclerosis showed palpable fibrous necrosis and calcification with elevated blood SDC-1 levels. miR-19a-3p could bind to SDC-1. Overexpression of miR19a-3p promoted cell proliferation, inhibited apoptosis and cholesterol efflux, down-regulated the expression of SDC-1, ABCA1, ABCG1, TGF-& beta;1 and p-Smad3 proteins in ox-LDL-induced HAECs. In conclusion, miR19a-3p targeting SDC-1 inhibited the ox-LDL-induced activation of the TGF-& beta;1/Smad3 pathway in HAECs.
期刊：
Journal of Pharmaceutical and Biomedical Analysis,2023年236:115656 ISSN：0731-7085
通讯作者：
Zhu, Liguo;Yang, SF
作者机构：
[Zhan, Jiawen; Wang, Shangquan; Li, Linghui; Sun, Wu; Chen, Ming; Yang, Shaofeng; Chen, Xin; Li, Kaiming; Zhu, Liguo; Yu, Jie; Gu, Jinyu; Feng, Minshan; Yin, Xunlu] China Acad Chinese Med Sci, Wangjing Hosp, Dept Gen Orthoped, Huajiadi St, Beijing 100102, Peoples R China.;[Wei, Xu] China Acad Chinese Med Sci, Wangjing Hosp, Dept Acad Dev, Huajiadi St, Beijing 100102, Peoples R China.;[Gong, Hao] Changping Hosp Integrated Chinese & Western Med, Dept Orthopaed, 219 Huangping St, Beijing 102208, Peoples R China.;[Yang, Shaofeng] Hunan Univ Chinese Med, Dept Spine, Hosp 1, Changsha 410007, Peoples R China.
通讯机构：
[Yang, SF ; Zhu, LG] C;China Acad Chinese Med Sci, Wangjing Hosp, Dept Gen Orthoped, Huajiadi St, Beijing 100102, Peoples R China.;Hunan Univ Chinese Med, Dept Spine, Hosp 1, Changsha 410007, Peoples R China.
摘要：
Degeneration of the intervertebral disc is primarily caused by the loss of nucleus pulposus cells (NPCs) and extracellular matrix (ECM) (IDD). Bu-Shen-Huo-Xue-Fang (BSHXF), a traditional Chinese medicine decoction, has been used to treat IDD in clinical; nevertheless, the active components and underlying molecular mechanisms remain unknown. BSHXF improved IL-113 and H2O2 stimulation-induced injuries on NPCs by promoting cell viability, increasing ECM deposition, inhibiting cell senescence, and decreasing the levels of inflammatory factors. The active ingredients in BSHXF were identified by LC-MS/MS analysis; three active ingredients from the principal drugs, Aucubin, Tanshinol, and Tanshinone II A promoted NPC viability; and Aucubin and Tanshinol promoted NPC viability more. Aucubin and Tanshinol, respectively, improved H2O2 stimulation-induced injuries on NPCs by promoting cell viability, increasing ECM deposition, inhibiting cell senescence, and decreasing the levels of inflammatory factors. The activator of NF-cB and Wnt signaling pathways attenuated Aucubin and Tanshinol's protective effects by promoting ECM degradation and NPC senescence. Aucubin, Tanshinol, and Tanshinone II A were identified as the most potent compounds in BSHXF protection against degenerative changes in NPCs. The NF-cB and Wnt signaling pathways might be involved in the protective effects of Aucubin and Tanshinol against H2O2-induced degenerative changes.
摘要：
The study uses an LMJSS‐TMT‐UPLC‐MS method to spatially map amine metabolites to brain histopathological changes including neuron loss, glial cell activation, and neurogenesis. This integrated study explains the mechanisms of posttraumatic brain injury brain damage and repair. It also uncovers the therapeutic mechanisms of traditional Chinese medicine, Xuefu Zhuyu decoction. Abstract Introduction Spatial changes of amine metabolites and histopathology of the whole brain help to reveal the mechanism of traumatic brain injury (TBI) and treatment. Methods A newly developed liquid microjunction surface sampling–tandem mass tag–ultra performance liquid chromatography–mass spectrometry technique is applied to profile brain amine metabolites in five brain regions after impact‐induced TBI at the subacute stage. H&E, Nissl, and immunofluorescence staining are performed to spatially correlate microscopical changes to metabolic alterations. Then, bioinformatics, molecular docking, ELISA, western blot, and immunofluorescence are integrated to uncover the mechanism of Xuefu Zhuyu decoction (XFZYD) against TBI. Results Besides the hippocampus and cortex, the thalamus, caudate‐putamen, and fiber tracts also show differentiated metabolic changes between the Sham and TBI groups. Fourteen amine metabolites (including isomers such as L‐leucine and L‐isoleucine) are significantly altered in specific regions. The metabolic changes are well matched with the degree of neuronal damage, glia activation, and neurorestoration. XFZYD reverses the dysregulation of several amine metabolites, such as hippocampal Lys‐Phe/Phe‐Lys and dopamine. Also, XFZYD enhances post‐TBI angiogenesis in the hippocampus and the thalamus. Conclusion This study reveals the local amine‐metabolite and histological changes in the subacute stage of TBI. XFZYD may promote TBI recovery by normalizing amine metabolites and spatially promoting dopamine production and angiogenesis.
摘要：
Background: How to comprehensively and objectively evaluate the quality, safety and efficacy of foods has always been a challenge in the field of food analysis. Molecular networking is an efficient analytical tool used for systematically profiling the components of food samples. It has various significant applications in food metabolomics, including systematic analysis and chemotaxonomic annotation of food ingredients, identification of quality markers of various foods, detection and quantification of toxicants and contaminants in foods, and guiding the development of new food products. Scope and approach: Herein, we summarized the recent progress in molecular networking in foodomics. Notably, this review provides an overview of the concepts, workflows, and applications of molecular networking in food and metabolomics. The potential and limitations of molecular networking are also analyzed. Ultimately, the current challenges and future developments of molecular networking in foodomics are briefly discussed. Key findings and conclusions: Molecular networking is a valuable tool for monitoring the quality and safety of foods and evaluating food function in food metabolomics, as well as accelerating the development of functional food products. However, further research is needed to validate these laboratory findings and apply them into industrialized food production and real-world applications.
作者机构：
[Zhou, Hao] Hunan Univ Chinese Med, Affiliated Hosp 2, Dept Urol, Changsha 410001, Hunan, Peoples R China.;[Wang, Fang] Changsha Social Work Coll, Med Coll, Changsha 410004, Hunan, Peoples R China.;[Wang, F; Wang, Fang] Changsha Social Work Coll, Med Coll, 22 XiangZhang Rd, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Wang, F ] C;Changsha Social Work Coll, Med Coll, 22 XiangZhang Rd, Changsha 410004, Hunan, Peoples R China.
摘要：
Hepatic leukemia factor (HLF), a transcription factor, is dysregulated in many cancers. This study investigates the function of HLF in prostate cancer (PCa) and its relation to tensin 1 (TNS1). Clinical tissues were collected from 24 PCa patients. Duke University 145 (DU145) and PC3 cells overexpressing HLF were established. HLF signaling was downregulated in PCa tissues compared to adjacent tissues and in DU145 and PC3 cells compared to prostate epithelial cells RWPE-1 or prostate stromal cells (WPMY-1). PCa cell lines with overexpression of HLF had reduced proliferative, migratory, and invasive activity, increased apoptosis, and cell mitosis mostly in the G0/G1 phase. HLF induced the TNS1 transcription to activate the p53 pathway. Depletion of TNS1 reversed the anti-tumor effects of HLF on PCa cells and tumor growth and metastasis in vivo. In summary, our findings suggest that HLF suppressed PCa progression by upregulating TNS1 expression and inducing the p53 pathway activation, which might provide insights into novel strategies for combating PCa.
摘要：
Background: Intracranial teratomas or other cystic lesions with atypical imaging manifestations can still be frequently seen clinically. The specific reasons for unusual imaging manifestations need to be further explored.Observation(s): A case of adult teratoma in the posterior fossa with unusual imaging manifesta-tions was reported. The chemical composition of its cystic fluid was quantitatively detected, and in vitro imaging simulation experiments were performed on some fluid substances with similar cystic fluid properties to explore the reasons for special imaging manifestations. The content of inorganic substances and protein in the cystic fluid were both low, with no melanin detected. In vitro experiments revealed that MR T1 signals could increase with protein content rising and changes in MR T2 signals presented no obvious correlation with it. CT values increased gradually with protein concentration rising. The substances with similar viscosity had similar CT values, whereas substance viscosity showed no significant correlation with changes in MR signals.Conclusion: The abnormality of imaging manifestations cannot be confirmed as the result of "high protein content", nor can it be simply attributed to bleeding. Further research is required for the impact of the combination of paramagnetic particles and biofluid on imaging.
通讯机构：
[Jianbo Liu] D;Department of Child Psychiatry of Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen Institute of Mental Health, Shenzhen Key Laboratory of Mental Health, Shenzhen, China
摘要：
Essential proteins play important roles in the development and survival of organisms whose mutations are proven to be the drivers of common internal diseases having higher prevalence rates. Due to high costs of traditional biological experiments, an improved Transfer Neural Network (TNN) was designed to extract raw features from multiple biological information of proteins first, and then, based on the newly-constructed Transfer Neural Network, a novel computational model called TNNM was designed to infer essential proteins in this paper. Different from traditional Markov chain, since Transfer Neural Network adopted the gradient descent algorithm to automatically obtain the transition probability matrix, the prediction accuracy of TNNM was greatly improved. Moreover, additional antecedent memory coefficient and bias term were introduced in Transfer Neural Network, which further enhanced both the robustness and the non-linear expression ability of TNNM as well. Finally, in order to evaluate the identification performance of TNNM, intensive experiments have been executed based on two well-known public databases separately, and experimental results show that TNNM can achieve better performance than representative state-of-the-art prediction models in terms of both predictive accuracies and decline rate of accuracies. Therefore, TNNM may play an important role in key protein prediction in the future.
期刊：
Chinese Medicine,2023年18(1):1-22 ISSN：1749-8546
通讯作者：
Zhang, SS
作者机构：
[Sheng, Qi; Zhang, Dingbang; Chen, Hongzhou; Cui, Tao; Li, Minghao; Liu, Xiaoqin; Zhang, Shuosheng; Zhang, Fayun; Meng, Xianglong; Li, Bin; Zhang, SS; Zhang, Jia] Shanxi Univ Chinese Med, Jinzhong 030619, Shanxi, Peoples R China.;[Liu, Xiaoqin; Zhang, Shuosheng; Meng, Xianglong; Zhang, SS] Shanxi Key Lab Tradit Herbal Med Proc, Jinzhong 030619, Shanxi, Peoples R China.;[Tan, Jiaying] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha 410021, Hunan, Peoples R China.;[Sheng, Qi] Guangxi Univ Chinese Med, Nanning 530001, Guangxi, Peoples R China.
通讯机构：
[Zhang, SS ] S;Shanxi Univ Chinese Med, Jinzhong 030619, Shanxi, Peoples R China.;Shanxi Key Lab Tradit Herbal Med Proc, Jinzhong 030619, Shanxi, Peoples R China.
关键词：
Xiaoke;Diabetes mellitus;Traditional Chinese medicine;Etiology and pathogenesis;Treatment guidelines
摘要：
Diabetes mellitus (DM) is a chronic metabolic disorder characterized by hyperglycemia resulting from insulin secretion defects or insulin resistance. The global incidence of DM has been gradually increasing due to improvements in living standards and changes in dietary habits, making it a major non-communicable disease that poses a significant threat to human health and life. The pathogenesis of DM remains incompletely understood till now, and current pharmacotherapeutic interventions are largely inadequate, resulting in relapses and severe adverse reactions. Although DM is not explicitly mentioned in traditional Chinese medicine (TCM) theory and clinical practice, it is often classified as “Xiaoke” due to similarities in etiology, pathogenesis, and symptoms. With its overall regulation, multiple targets, and personalized medication approach, TCM treatment can effectively alleviate the clinical manifestations of DM and prevent or treat its complications. Furthermore, TCM exhibits desirable therapeutic effects with minimal side effects and a favorable safety profile. This paper provides a comprehensive comparison and contrast of Xiaoke and DM by examining the involvement of TCM in their etiology, pathogenesis, treatment guidelines, and other relevant aspects based on classical literature and research reports. The current TCM experimental research on the treatment of DM by lowering blood glucose levels also be generalized. This innovative focus not only illuminates the role of TCM in DM treatment, but also underscores the potential of TCM in DM management.
