作者机构：
[Hao-Ran Peng; Jie Yu; Zhi-Min Yu; Zhi-Min Jiang; Ting-Ting Yan] Kunming University of Science and Technology, Yunnan 650093, China;[Qiang Zhao] Department of Stomatology, 920 Hospital of Joint Logistics Support Force, PLA, Kunming 650032, China;[Ting Xiao] Changsha Stomatological Hospital, School of Dental Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, China
通讯机构：
[Ting-Ting Yan] K;Kunming University of Science and Technology, Yunnan 650093, China
摘要：
As one of the commonly used methods for healing bone defects, autologous bone transplantation is limited due to the scarcity of bone sources and the side effects. Therefore, bone tissue engineering scaffolds are widely used as bone repair materials. Research has shown that the addition of trace elements can improve the osteogenic performance of hydroxyapatite, such as magnesium and strontium ions that can promote bone mineralization and osteoblast proliferation, and promote the formation of new bone in vitro and in vivo tests. The main research results of this paper are as follows: (1) The pore size of the scaffold is distributed between 10 and 70 μm, and it also has a through hole structure of 300–500 μm; (2) Whisker scaffolders had no obvious cytotoxicity, and the addition of Mg and Sr ions would increase the cell proliferation; (3) The degradation rate of the scaffold is relatively slow; (4) ALP shows that pure hydroxyapatite whisker scaffolds have bone mineralization ability, and magnesium and strontium ions can enhance the bone mineralization ability of hydroxyapatite.
通讯机构：
[Tang, ZL; Qiu, RH ; Chen, Y ] H;Hunan Univ Sci & Technol, Sch Chem & Chem Engn, Key Lab Theoret Organ Chem & Funct Mol, Minist Educ,Hunan Prov Key Lab Controllable Prepar, Xiangtan 411201, Hunan, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Adv Catalyt Engn Res Ctr,Minist Educ, Changsha 410082, Peoples R China.;Hunan Univ Chinese Med, Sch Med, Dept Physiol, Changsha 410208, Hunan, Peoples R China.
摘要：
A Sb,N ligand (L-Sb) for Pd-catalyzed double N-arylation of primary amines was developed. This trivalent ligand L-Sb, containing a 5,6,7,12-tetrahydrodibenzo[c,f][1,5]azastibocine skeleton and stable under air and moisture, could be synthesized facilely on a gram scale from chlorostibine (1) and cyclopentylmagnesium bromide. L-Sb showed excellent catalytic performance in Pd-2(dba)(3)-catalyzed double N-arylation of 2,2 '-dibromo-1,1 '-biphenyl (2) with primary amines (3), affording functionalized carbazoles in good yields. This Pd-2(dba)(3)/L-Sb-catalyzed double N-arylation, the first example of the application of trivalent organostibines as a ligand in N-arylation, featured the following advantages: small catalyst loading, wide functional group tolerance, good yields, and ease of gram-scale synthesis.
通讯机构：
[Qiu, RH ; Xiong, BQ ; Chen, Y ] H;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan Univ Chinese Med, Sch Med, Dept Physiol, Changsha 410208, Peoples R China.;Hunan Inst Sci & Technol, Dept Chem & Chem Engn, Yueyang 414006, Peoples R China.
摘要：
In this study, we present a nickel-catalyzed reductive C-(sp(3))-Sb coupling of unactivated alkyl chlorides with chlorostibines. This approach is highly versatile, tolerating various functional groups such as acetal, alkene, nitrile, amine, ester, silyl ether, thioether, and various heterocyclic compounds. Notably, the late-stage modification of bioactive molecules and the satisfactory anticancer activity against cancerous MDA-MB-231 also demonstrate the potential application.
摘要：
BACKGROUND: Hyperglycemia is widespread in the world's population, increasing the risk of many diseases. This study aimed to explore the regulatory effect and mechanism of astragaloside IV (ASIV)-mediated endothelial progenitor cells (EPCs) exosomal LINC01963 in endothelial cells (HUVECs) impaired by high glucose. METHODS: Morphologies of exosomes were observed by light microscope and electron microscope. Immunofluorescence was used to identify EPCs and detect the expressions of caspase-1. LINC01963 was detected by quantitative reverse transcription PCR. NLRP3, ASC, and caspase-3 were detected by Western Blot. Nanoparticle tracking analysis was carried out to analyze the exosome diameter. High-throughput sequencing was applied to screen target lncRNAs. The proliferation of endothelial cells was measured by cell counting kit-8 assay. The apoptosis level of HUVECs was detected by flow cytometry and TdT-mediated dUTP Nick-End labeling. The levels of IL-1β, IL-18, ROS, SOD, MDA, and LDH were measured by enzyme-linked immunosorbent assay. RESULTS: ASIV could promote the secretion of the EPC exosome. LINC01963 was obtained by high-throughput sequencing. It was observed that high glucose could inhibit the proliferation, reduce the level of SOD, the expression of NLRP3, ASC, and caspase-1, increase the levels of IL-1β, IL-18, ROS, MDA, and LDH, and promote apoptosis of HUVECs. Whereas LINC01963 could inhibit the apoptosis of HUVECs, the expression of NLRP3, ASC, and caspase-1 increased, and the levels of IL-1β, IL-18, ROS, MDA, and LDH decreased. CONCLUSION: EPCs exosomal LINC01963 play an inhibitory role in high glucose-induced pyroptosis and oxidative stress of HUVECs. This study provides new ideas and directions for treating hyperglycemia and researching exosomal lncRNAs.
通讯机构：
[Wu, P; Lin, LM ] H;Hunan Univ Chinese Med, Coll Pharm, 300 Xueshi Rd, Changsha 410208, Peoples R China.
关键词：
AMPK;Diabetes mellitus;Medicinal and edible homologous;PI3K/Akt;Plant polysaccharides
摘要：
Medicinal and edible homologs (MEHs) can be used in medicine and food. The National Health Commission announced that a total of 103 kinds of medicinal and edible homologous plants (MEHPs) would be available by were available in 2023. Diabetes mellitus (DM) has become the third most common chronic metabolic disease that seriously threatens human health worldwide. Polysaccharides, the main component isolated from MEHPs, have significant antidiabetic effects with few side effects. Based on a literature search, this paper summarizes the preparation methods, structural characterization, and antidiabetic functions and mechanisms of MEHPs polysaccharides (MEHPPs). Specifically, MEHPPs mainly regulate PI3K/Akt, AMPK, cAMP/PKA, Nrf2/Keap1, NF-κB, MAPK and other signaling pathways to promote insulin secretion and release, improve glycolipid metabolism, inhibit the inflammatory response, decrease oxidative stress and regulate intestinal flora. Among them, 16 kinds of MEHPPs were found to have obvious anti-diabetic effects. This article reviews the prevention and treatment of diabetes and its complications by MEHPPs and provides a basis for the development of safe and effective MEHPP-derived health products and new drugs to prevent and treat diabetes.
期刊：
TISSUE ENGINEERING AND REGENERATIVE MEDICINE,2024年21(1):185-197 ISSN：1738-2696
通讯作者：
Zhang, Ying;Cao, XY
作者机构：
[Zhang, Leilei; Cao, XY; Zhang, Ying; Cao, Xiangyang] Luoyang Orthoped Traumatol Hosp, Orthoped Hosp Henan Prov, Med Ctr Hip, 82 Qiming South Rd, Luoyang 471002, Henan, Peoples R China.;[Li, Chuan] 920Th Hosp Joint Logist Support Force, Dept Oncol, Kunming 650032, Yunnan, Peoples R China.;[Wei, Qiushi; He, Wei] Guangzhou Univ Tradit Chinese Med, Inst Orthopaed, Guangzhou 510240, Peoples R China.;[Wei, Qiushi; He, Wei] Guangzhou Univ Tradit Chinese Med, Affiliated Hosp 3, Guangzhou 510240, Peoples R China.;[Jing, Zhenhao; Yuan, Qiang; Dong, Yiping] Henan Univ Tradit Chinese Med, Zhengzhou 450046, Henan, Peoples R China.
通讯机构：
[Cao, XY ; Zhang, Y] L;Luoyang Orthoped Traumatol Hosp, Orthoped Hosp Henan Prov, Med Ctr Hip, 82 Qiming South Rd, Luoyang 471002, Henan, Peoples R China.
关键词：
Osteonecrosis of the femoral head;Sustained release system;PEG-PLGA-PLL nanoparticle;MiR-320a;Bone regeneration
摘要：
BACKGROUND: This study aimed to explore the effect of a nanomaterial-based miR-320a inhibitor sustained release system in trauma-induced osteonecrosis of the femoral head (TIONFH). METHODS: The miR-320a inhibitor-loaded polyethylene glycol (PEG)- Poly(lactic-co-glycolic acid) (PLGA)- Poly-L-lysine (PLL) nanoparticles were constructed using the double emulsion method. The TIONFH rabbit model was established to observe the effects of miR-320a inhibitor nanoparticles in vivo. Hematoxylin-eosin staining and microcomputed tomography scanning were used for bone morphology analysis. Bone marrow mesenchymal stem cells (BMSCs), derived from TIONFH rabbits, were used for in vitro experiments. Cell viability was determined using the MTT assay. RESULTS: High expression of miR-320a inhibited the osteogenic differentiation capacity of BMSCs in vitro by inhibiting the expression of the osteoblastic differentiation markers ALP and RUNX2. MiR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticles were constructed with a mean loading efficiency of 1.414 ± 0.160%, and a mean encapsulation efficiency of 93.45 ± 1.24%, which released 50% of the loaded miR-320a inhibitor at day 12 and 80% on day 18. Then, inhibitor release entered the plateau. After treatment with the miR-320a inhibitor nanoparticle, the empty lacunae were decreased in the femoral head tissue of TIONFH rabbits, and the osteoblast surface/bone surface (Ob.S/BS), osteoblast number/bone perimeter (Ob.N/B.Pm), bone volume fraction, and bone mineral density increased. Additionally, the expression of osteogenic markers RUNX2 and ALP was significantly elevated in the TIONFH rabbit model. CONCLUSION: The miR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticle sustained drug release system significantly contributed to bone regeneration in the TIONFH rabbit model, which might be a promising strategy for the treatment of TIONFH.
