期刊:
Frontiers in Pharmacology,2023年14:1173747 ISSN:1663-9812
通讯作者:
Long, HP
作者机构:
[Zhou, Siqian; Long, HP; Liu, Jian; Long, Hongping] Hunan Univ Chinese Med, Ctr Med Res & Innovat, Hosp 1, Changsha, Peoples R China.;[Ding, Changsong; Zhou, Siqian; Wang, Yajing; Long, HP; Long, Hongping] Hunan Univ Chinese Med, Changsha, Peoples R China.;[Tan, Leihong] Hunan Univ Chinese Med, Dept Pharm, Hosp 2, Changsha, Peoples R China.;[Wang, Yikun] Cent South Univ, Xiangya Hosp 2, Dept Pharm, Changsha, Peoples R China.;[Li, Jing] Cent South Univ, Xiangya Hosp, Dept Pharm, Changsha, Peoples R China.
通讯机构:
[Long, HP ] H;Hunan Univ Chinese Med, Ctr Med Res & Innovat, Hosp 1, Changsha, Peoples R China.;Hunan Univ Chinese Med, Changsha, Peoples R China.
摘要:
Introduction: Corni Fructus (CF) is a Chinese herbal medicine used for medicinal and dietary purposes. It is available commercially in two main forms: raw CF (unprocessed CF) and wine-processed CF. Clinical observations have indicated that wine-processed CF exhibits superior hypoglycemic activity compared to its raw counterpart. However, the mechanisms responsible for this improvement are not well understood. Methods: To address this gap in knowledge, we conducted metabolomics analysis using ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-QTOF-MS) to compare the chemical composition of raw CF and wine-processed CF. Subsequently, network analysis, along with immunofluorescence assays, was employed to elucidate the potential targets and mechanisms underlying the hypoglycemic effects of metabolites in CF. Results: Our results revealed significant compositional differences between raw CF and wine-processed CF, identifying 34 potential markers for distinguishing between the two forms of CF. Notably, wine processing led to a marked decrease in iridoid glycosides and flavonoid glycosides, which are abundant in raw CF. Network analysis predictions provided clues that eight compounds might serve as hypoglycemic metabolites of CF, and glucokinase (GCK) and adenylate cyclase (ADCYs) were speculated as possible key targets responsible for the hypoglycemic effects of CF. Immunofluorescence assays confirmed that oleanolic acid and ursolic acid, two bioactive compounds present in CF, significantly upregulated the expression of GCK and ADCYs in the HepG2 cell model. Discussion: These findings support the notion that CF exerted hypoglycemic activity via multiple components and targets, shedding light on the impact of processing methods on the chemical composition and hypoglycemic activity of Chinese herbal medicine.
期刊:
Frontiers in Public Health,2023年11:1293134 ISSN:2296-2565
通讯作者:
Song, Zhenyan;Cheng, SW
作者机构:
[Song, Zhenyan; Guo, Minhua; Cheng, Shaowu; Song, ZY; He, Jiawei; Cheng, SW; Wang, Shiwei] Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Changsha, Peoples R China.;[Song, Zhenyan; Guo, Minhua; Cheng, Shaowu; Song, ZY; He, Jiawei; Cheng, SW; Wang, Shiwei] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha 410128, Peoples R China.;[Wang, Weijie] Hunan Univ Chinese Med, Sch Informat, Changsha, Peoples R China.
通讯机构:
[Song, ZY; Cheng, SW ] H;Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Changsha, Peoples R China.;Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha 410128, Peoples R China.
关键词:
Barthel Index (BI);activities of daily living;machine learning algorithm;memory-related diseases;the China health and retirement longitudinal survey
摘要:
INTRODUCTION: Memory-related diseases (MDs) pose a significant healthcare challenge globally, and early detection is essential for effective intervention. This study investigates the potential of Activities of Daily Living (ADL) as a clinical diagnostic indicator for MDs. Utilizing data from the 2018 national baseline survey of the China Health and Retirement Longitudinal Study (CHARLS), encompassing 10,062 Chinese individuals aged 45 or older, we assessed ADL using the Barthel Index (BI) and correlated it with the presence of MDs. Statistical analysis, supplemented by machine learning algorithms (Support Vector Machine, Decision Tree, and Logistic Regression), was employed to elucidate the relationship between ADL and MDs. BACKGROUND: MDs represent a significant public health concern, necessitating early detection and intervention to mitigate their impact on individuals and society. Identifying reliable clinical diagnostic signs for MDs is imperative. ADL have garnered attention as a potential marker. This study aims to rigorously analyze clinical data and validate machine learning algorithms to ascertain if ADL can serve as an indicator of MDs. METHODS: Data from the 2018 national baseline survey of the China Health and Retirement Longitudinal Study (CHARLS) were employed, encompassing responses from 10,062 Chinese individuals aged 45 or older. ADL was assessed using the BI, while the presence of MDs was determined through health report questions. Statistical analysis was executed using SPSS 25.0, and machine learning algorithms, including Support Vector Machine (SVM), Decision Tree Learning (DT), and Logistic Regression (LR), were implemented using Python 3.10.2. RESULTS: Population characteristics analysis revealed that the average BI score for individuals with MDs was 70.88, significantly lower than the average score of 87.77 in the control group. Pearson's correlation analysis demonstrated a robust negative association (r = -0.188, p < 0.001) between ADL and MDs. After adjusting for covariates such as gender, age, smoking status, drinking status, hypertension, diabetes, and dyslipidemia, the negative relationship between ADL and MDs remained statistically significant (B = -0.002, β = -0.142, t = -14.393, 95% CI = -0.002, -0.001, p = 0.000). The application of machine learning models further confirmed the predictive accuracy of ADL for MDs, with area under the curve (AUC) values as follows: SVM-AUC = 0.69, DT-AUC = 0.715, LR-AUC = 0.7. Comparative analysis of machine learning outcomes with and without the BI underscored the BI's role in enhancing predictive abilities, with the DT model demonstrating superior performance. CONCLUSION: This study establishes a robust negative correlation between ADL and MDs through comprehensive statistical analysis and machine learning algorithms. The results validate ADL as a promising diagnostic indicator for MDs, with enhanced predictive accuracy when coupled with the Barthel Index. Lower levels of ADL are associated with an increased likelihood of developing memory-related diseases, underscoring the clinical relevance of ADL assessment in early disease detection.
作者机构:
[Lin, Mei-yu; Wang, Hui-qin; Yang, Yan-tao] Hunan Univ Chinese Med, Changsha, Peoples R China.;[Chen, Nai-hong] Chinese Acad Med Sci & Peking Union Med Coll, Inst Mat Med, Beijing, Peoples R China.;[Chen, Nai-hong] Chinese Acad Med Sci & Peking Union Med Coll, Neurosci Ctr, Beijing, Peoples R China.
会议名称:
19th World Congress of Basic and Clinical Pharmacology (WCP)
摘要:
There are different views of how the immune system participates in the reaction to cancer. Here, we evaluated expression of DAMP proteins HSP70 and cancer-testis antigen SPAG9 in patients with hepatocellular carcinoma(HCC) and lung cancer to explore tumor immunity. Our analysis showed that levels of HSP70 and SPAG9 antibody were significantly higher in serum of lung cancer and HCC patients than in serum from healthy subjects(P < 0.001), but there were no differences in levels of HSP70 antibody in patients and controls. Levels of serum SPAG9 antibody in newly diagnosed lung cancer patients were significantly higher than in treated lung cancer patients(P < 0.05), but there were no differences in levels of HSP70 or HSP70 antibody. Levels of serum HSP70 and SPAG9 antibody, but not HSP70 antibody, were also higher in hepatitis/cirrhosis patients than in healthy subjects(P = 0.005, P < 0.001). Levels of serum SPAG9 antibody were significantly higher in HCC patients than in hepatitis/cirrhosis patients, but there were no differences in HSP70 or HSP70 antibody levels. Finally, levels of serum HSP70 and SPAG9 antibody were significantly higher in HCC patients than in lung cancer patients(P < 0.05, P < 0.001). These results indicate that cancer-testis antigen SPAG9 induces a strong humoral immune response in cancer patients but HSP70 does not. These results show that SPAG9 has potential as a tumor-specific biomarker.
作者机构:
[Liu, Yu] Hunan Univ Chinese Med, Coll Acupuncture & Moxibust & Tui Na, Changsha, Peoples R China.;[Gong, Houwu] DHC SOFTWARE CO LTD, DHC Mediway Technol Co Ltd, Beijing, Peoples R China.;[Chen, Heng] Hunan Univ Chinese Med, Affiliated Hosp 2, Dept Encephalopathy, Changsha, Peoples R China.
会议名称:
IEEE International Conference on Bioinformatics and Biomedicine (BIBM)
会议时间:
NOV 18-21, 2019
会议地点:
San Diego, CA
会议主办单位:
[Liu, Yu] Hunan Univ Chinese Med, Coll Acupuncture & Moxibust & Tui Na, Changsha, Peoples R China.^[Gong, Houwu] DHC SOFTWARE CO LTD, DHC Mediway Technol Co Ltd, Beijing, Peoples R China.^[Chen, Heng] Hunan Univ Chinese Med, Affiliated Hosp 2, Dept Encephalopathy, Changsha, Peoples R China.
会议论文集名称:
IEEE International Conference on Bioinformatics and Biomedicine-BIBM
关键词:
Xievin Decoction Analogous Prescriptions;Xinkai Kujiang therapy(herbs acrid in flavor for dispersing and herbs bitter in flavorfor descending);complex network;data mining
摘要:
Objective To summarize the core drugs, compatibility rules and properties, flavor and meridian tropism of the Xiexin Decoction Analogous Prescriptions by complex network mining techniques, in order to provide reference for the research of the Analogous prescriptions. Methods The prescription information was extracted from the case of Xiexin Decoction Analogous Prescription(herbs acrid in flavor for dispersing and herbs bitter in flavor for descending)in the the "Ancient and Modern Medical Case Cloud Platform", and the core drugs, compatibility rules and properties,flavor and meridian tropism of the Xiexin Decoction Analogous Prescriptions were analyzed. Results The core drugs of Xiexin Decoction Analogous Prescription were Pinellia, Dried Ginger, Licorice, Rhizoma Coptidis, Scutellaria, Poria, Codonopsis, Jujube, Radix Paeoniae Alba, Atractylodes Rhizome, Tangerine, Amomum vulgaris. The herbs especially belonged to the warm properties,the acrid flavor, and the meridian tropism of these drugs belonged to spleen and stomach channels. Excavation of the common drugs on the compatibility and network compatibility group are: Scutellaria-Coptis, Dried ginger - Pinellia, Jujube - Licorice - Codonopsis, Berberine+Scutellaria Pinellia+Dried ginger. Conclusion Using the complex network mining technology, the core drugs, compatibility drugs and properties, flavor and meridian tropism of the Xiexin Decoction Analogous Prescription were obtained, which played an exemplary role in further digging into the compatibility of the analogous prescriptions.