利用分子信标和EXO III放大荧光信号快速检测Ag~+
作者:
贺气志;罗怀青;陈珂珂;徐倩;唐亮;...
期刊:
中国环境科学 ,2018年38(2):737-744 ISSN:1000-6923
通讯作者:
Ning, Yi(121407735@qq.com)
作者机构:
[陈珂珂; 唐亮; 贺气志; 罗怀青; 徐倩] School of Basic Medical Science, Changsha Medical University, Changsha, 410219, China;[宁毅] The Medicine School of Hunan University of Chinese Medicine, Changsha, 410208, China
通讯机构:
The Medicine School of Hunan University of Chinese Medicine, Changsha, China
关键词:
分子信标;探针;荧光值
摘要:
设计新型的DNA探针,其主要功能区域包含适配体区域和分子信标互补区域;当无靶标存在时,羧基荧光素(FAM)的荧光被黑洞猝灭基团(BHQ)猝灭,加入靶标后,Ag~+与适配体区域特异性结合,使得分子信标与其互补区域发生结合,加入EXO III后,分子信标被酶解,从而释放Ag~+/DNA探针复合物用于下一轮反应,同时产生大量的FAM,因此,可以通过荧光的增强来定量检测靶标的含量.评价了该方法的灵敏度和选择性,探究它在实际样品中的表现能力.结果表明,适配体区域与分子信标间隔2个碱基的DNA探针与分子信标结合效果最好,检测的信噪比最大.该方法对Ag~+的检测最低下限(LOD)为0.1nmol/L,在0.5~200nmol/L范围内,荧光值与靶标浓度成线性关系(R~2 = 0.994).对实际样品的检测在20~200nmOl/L范围内呈线性关系(R~2=0.998),且能很好的从混合样品中区分10nmol/L Ag~+.该研究建立的快速检测Ag~+方法具有良好的线性定量能力和操作性能,且灵敏度高、特异性强,可满足现实的需求.
语种:
中文
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Combat biofilm by bacteriostatic aptamer-functionalized graphene oxide
作者:
Mao, Biyao;Cheng, Lijuan
( 程莉娟 ) ;Wang, Sheng;Zhou, Jiaqi;Deng, Le
*
期刊:
Biotechnology and Applied Biochemistry ,2018年65(3):355-361 ISSN:0885-4513
通讯作者:
Deng, Le
作者机构:
[Cheng, Lijuan; Zhou, Jiaqi; Mao, Biyao; Deng, Le; Wang, Sheng] Hunan Normal Univ, State Key Lab Dev Biol Freshwater Fish, Coll Life Sci, Changsha, Hunan, Peoples R China.;[Cheng, Lijuan] Hunan Univ Chinese Med, Dept Biochem & Mol Biol, Changsha, Hunan, Peoples R China.
通讯机构:
[Deng, Le] H;Hunan Normal Univ, State Key Lab Dev Biol Freshwater Fish, Coll Life Sci, Changsha, Hunan, Peoples R China.
关键词:
biofilm;conjugate;Salmonella typhimurium;target delivery
摘要:
Biofilms are the main reason for a large number deaths and high health costs. Their better protection compared to planktonic form against conventional antibiotics leads to poor treatment efficiency. Nanoagent-targeted delivery is a promising avenue for disease therapeutic, but its application targeting biofilms has not been reported currently. The roles, if any, of aptamers acting as delivery carrier and targeting factor, the graphene oxide (GO), and GO modified with aptamers against biofilms were then systematically evaluated. Here, we successfully developed an aptamer-targeted GO strategy against biofilms. We investigated the efficacy of aptamer–GO conjugates by UV spectrophotometer, inverted microscopy, and atomic force microscopy; 93.5 ± 3.4% Salmonella typhimurium biofilms were inhibited and 84.6 ± 5.1% of biofilms were dispersed by a ST-3-GO conjugate. More importantly, this conjugate represented distinctively toxicity to S. typhimurium. Thus, this strategy significantly displays excellent antibiofilm properties and may serve as a long-term solution for biofilm control. © 2018 International Union of Biochemistry and Molecular Biology, Inc.
语种:
英文
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A highly selective ESIPT-based fluorescent probe for cysteine sensing and its bioimaging application in living cells
作者:
Lv, Hao-Ming;Yuan, Ding-Hao;Liu, Wenping;Chen, Yi
* ( 陈懿 ) ;Au, Chak-Tong;...
期刊:
Sensors and Actuators B-Chemical ,2016年233:173-179 ISSN:0925-4005
通讯作者:
Yin, Shuang-Feng;Chen, Yi
( 陈懿 )
作者机构:
[Yin, Shuang-Feng; Lv, Hao-Ming; Yuan, Ding-Hao] Hunan Univ, Coll Chem & Chem Engn, Prov Hunan Key Lab Cost Effect Utilizat Fossil Fu, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Chen, Yi; Liu, Wenping] Hunan Univ Chinese Med, Coll Basic Med, Changsha 410208, Hunan, Peoples R China.;[Au, Chak-Tong] Hunan Inst Engn, Coll Chem & Chem Engn, Xiangtan 411104, Peoples R China.
通讯机构:
[Chen, Yi; Yin, Shuang-Feng] H;Hunan Univ, Coll Chem & Chem Engn, Prov Hunan Key Lab Cost Effect Utilizat Fossil Fu, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Hunan Univ Chinese Med, Coll Basic Med, Changsha 410208, Hunan, Peoples R China.
关键词:
Fluorescent probe;ESIPT;Cysteine;Live cell imaging
摘要:
A novel fluorescent probe, viz. PI-Cys, was developed on the basis of the excited state intramolecular proton transfer (ESIPT) mechanism for the detection of cysteine (Cys). It consists of an new ESIPT dye as the fluorophore, linked via a hydroxyl moiety to an acrylate group. The acrylate group acts as an ESIPT blocking agent as well as a functional recognition moiety. In the presence of Cys, the reaction of Cys with PI-Cys results in cleavage of the acrylate moiety from PI-Cys, thereby unleashing the ESIPT process and leading to about 20-fold enhancement of fluorescence. The probe exhibits high sensitivity and selectivity toward Cys over homocysteine and glutathione. Finally, the probe was successfully applied for fluorescence bioimaging of Cys in living cancer cells.
语种:
英文
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An ESIPT-based ratiometric fluorescent probe for the imaging of nitroxyl in living cells
作者:
Lv, Hao-Ming;Chen, Yi
( 陈懿 ) ;Lei, Jian;Au, Chak-Tong;Yin, Shuang-Feng
*
期刊:
Analytical Methods ,2015年7(9):3883-3887 ISSN:1759-9660
通讯作者:
Yin, Shuang-Feng
作者机构:
[Au, Chak-Tong; Lei, Jian; Yin, Shuang-Feng; Lv, Hao-Ming] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Chen, Yi] Hunan Univ Chinese Med, Med Coll, Changsha 410208, Hunan, Peoples R China.
通讯机构:
[Yin, Shuang-Feng] H;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
摘要:
Nitroxyl is a highly reactive compound that irreversibly dimerizes and subsequently dehydrates to yield nitrous oxide and water, making its direct detection in cells or in vivo difficult. In this paper, a novel fluorescent probe 1 based on the excited state intramolecular proton transfer (ESIPT) mechanism for the detection of nitroxyl was developed for the first time. It consists of 2-(2′-hydroxyphenyl)-benzothiazole as the fluorophore, linked via an ester moiety to a diphenylphosphinobenzoyl group. The probe exhibited high sensitivity and selectivity toward nitroxyl in the presence of biologically relevant species. Moreover, the potential for biological applications of the probe was confirmed by employing it for fluorescence imaging of nitroxyl in living cells. This journal is ©The Royal Society of Chemistry 2015.
语种:
英文
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非那雄胺/聚碳酸亚丙酯马来酸酯缓释微球的制备与性能
作者:
彭东明;张航;王晓红;刘艳飞
期刊:
中南大学学报(自然科学版) ,2014年45(7):2171-2175 ISSN:1672-7207
通讯作者:
Liu, Yanfei
作者机构:
[彭东明; 刘艳飞; 王晓红; 张航] School of Chemistry and Chemical Engineering, Central South University, Changsha, China;[彭东明] School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China
通讯机构:
School of Chemistry and Chemical Engineering, Central South University, Changsha, China
关键词:
非那雄胺;聚碳酸亚丙酯马来酸酯;缓释微球;药物传递体系
摘要:
以新型聚合物聚碳酸亚丙酯马来酸酯(PPCM)为载体,采用Oil/Water单乳液溶剂挥发法制备药物非那雄胺(finasteride)的缓释微球,并研究聚合物PPCM与药物finasteride的质量比对微球特性的影响。研究结果表明:所得PPCM微球外观圆整,平均粒径约为2 μm。随着非那雄胺比例的增加,微球的载药量提高,而药物的包封率则明显降低。在m(PPCM):m(finasteride)为5:1的条件下,获得较高的载药量和包封率,分别为14.78%和66.17%。在pH 7.4的磷酸盐缓冲溶液中,载药微球的体外释放时间达42 d,药物累积释放量为(92.59±2.62)%。微球的释药特性符合Higuchi方程Q_t=3.11+15.07 t~(1/2)。PPCM适用于长效缓释药物传递系统。
语种:
中文
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Organoantimony and organobismuth complexes for CO2 fixation
作者:
Chen, Yi
( 陈懿 ) ;Qiu, Renhua;Xu, Xinhua;Au, Chak-Tong;Yin, Shuang-Feng
*
期刊:
RSC Advances ,2014年4(23):11907-11918 ISSN:2046-2069
通讯作者:
Yin, Shuang-Feng
作者机构:
[Chen, Yi; Au, Chak-Tong; Yin, Shuang-Feng; Qiu, Renhua; Xu, Xinhua] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Chen, Yi] Hunan Univ Chinese Med, Coll Basic Med, Changsha 410208, Hunan, Peoples R China.;[Au, Chak-Tong] Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Hong Kong, Peoples R China.
通讯机构:
[Yin, Shuang-Feng] H;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
摘要:
The utilization of organoantimony (bismuth) complexes in CO<inf>2</inf> fixation is reviewed in this article. The efficient synthesis of cyclic carbonates from CO<inf>2</inf> and epoxides over an organoantimony(v) catalyst was first reported in 1979. After that, several organoantimony(v) complexes were found to be active for CO<inf>2</inf> fixation. In 2009, an organoantimony(iii) complex was reported as an effective CO<inf>2</inf> absorbent and it is the only example of this kind. The study of organobismuth complexes for CO <inf>2</inf> fixation started in 2008, and the achievements are: (i) when organobismuth oxides, hydroxides and methoxides are used as CO<inf>2</inf> absorbents, they are converted to carbonates, and the organobismuth carbonates can be renewed as organobismuth oxides;(ii) organobismuth complexes can be used as catalysts to transform CO<inf>2</inf> into epoxides as cyclic carbonates at room temperature in the presence of a co-catalyst such as LiI and Bu <inf>4</inf>NI;(iii) there is the development of bimetallic organobismuth complexes that show cooperative catalytic action;(iv) there is the physical fixation of CO<inf>2</inf> by inorganic-organic bismuth complexes;and (v) CO<inf>2</inf> insertion into the Bi-C bond of an organobismuth complex is disclosed. Most of the above catalytic systems can be considered as "electrophile-nucleophile". In general, the synthesis of cyclic carbonates from CO<inf>2</inf> and epoxides catalyzed by an organoantimony (bismuth) complex follows the mechanism: the complex first reacts with CO <inf>2</inf> to form an organometallic carbonate, then the carbonate reacts with an epoxide with ring-opening by a base (e.g., n-Bu<inf>4</inf>NI). ©The Royal Society of Chemistry 2014.
语种:
英文
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Analysis of the Interaction of Tarantula Toxin Jingzhaotoxin-III (beta-TRTX-Cj1 alpha) with the Voltage Sensor of Kv2.1 Uncovers the Molecular Basis for Cross-Activities on Kv2.1 and Nav1.5 Channels
作者:
Tao, Huai
( 陶怀 ) ;Chen, Jin J.;Xiao, Yu C.;Wu, Yuan Y.;Su, Hai B.;...
期刊:
Biochemistry ,2013年52(42):7439-7448 ISSN:0006-2960
通讯作者:
Liang, Song P.
作者机构:
[Tao, Huai; Wang, Heng Y.; Li, Dan; Liang, Song P.; Liu, Zhong H.; Wu, Yuan Y.; Su, Hai B.; Xiao, Yu C.; Wang, Mei C.] Hunan Normal Univ, Coll Life Sci, Key Lab Prot Chem & Dev Biol, Minist Educ, Changsha 410081, Hunan, Peoples R China.;[Tao, Huai] Hunan Univ Chinese Med, Dept Biochem & Mol Biol, Changsha 410208, Hunan, Peoples R China.;[Chen, Jin J.] Hunan Agr Univ, Coll Biol Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Deng, Mel C.] Cent S Univ, Sch Biol Sci & Technol, Dept Biochem, Changsha 410013, Hunan, Peoples R China.
通讯机构:
[Liang, Song P.] H;Hunan Normal Univ, Coll Life Sci, Key Lab Prot Chem & Dev Biol, Minist Educ, Changsha 410081, Hunan, Peoples R China.
摘要:
Animal venoms contain a fascinating array of divergent peptide toxins that have cross-activities on different types of voltage-gated ion channels. However, the underlying mechanism remains poorly understood. Jingzhaotoxin-III (JZTX-III), a 36-residue peptide from the tarantula Chilobrachys jingzhao, is specific for Nav1.5 and Kv2.1 channels over the majority of other ion channel subtypes. JZTX-III traps the Nav1.5 DII voltage sensor at closed state by binding to the DIIS3-S4 linker. In this study, electrophysiological experiments showed that JZTX-III had no effect on five voltage-gated potassium channel subtypes (Kv1.4, Kv3.1, and Kv4.1-4.3), whereas it significantly inhibited Kv2.1 with an IC<inf>50</inf> of 0.71 ±0.01 μM. Mutagenesis and modeling data suggested that JZTX-III docks at the Kv2.1 voltage-sensor paddle. Alanine replacement of Phe274, Lys280, Ser281, Leu283, Gln284, and Val288 could decrease JZTX-III affinity by 7-, 9-, 34-, 12-, 9-, and 7-fold, respectively. Among them, S281 is the most crucial determinant, and the substitution with Thr only slightly reduced toxin sensitivity. In contrast, a single conversion of Ser281 to Ala, Phe, Ile, Val, or Glu increased the IC<inf>50</inf> value by >34-fold. Alanine-scanning mutagenesis experiments indicated that the functional surface of JZTX-III bound to the Kv2.1 channel is composed of four hydrophobic residues (Trp8, Trp28, Trp30, and Val33) and three charged residues (Arg13, Lys15, and Glu34). The bioactive surfaces of JZTX-III interacting with Kv2.1 and Nav1.5 are only partially overlapping. These results strongly supported the hypothesis that animal toxins might use partially overlapping bioactive surfaces to target the voltage-sensor paddles of two different types of ion channels. Increasing our understanding of the molecular mechanisms of toxins interacting with voltage-gated sodium and potassium channels may provide new molecular insights into the design of more potent ion channel inhibitors. ©2013 American Chemical Society.
语种:
英文
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盐酸头孢吡肟缓释微球的制备与工艺优化
作者:
彭东明;王春燕;刘艳飞;李永欣;苏小爱;...
期刊:
中南大学学报(自然科学版) ,2013年44(3):907-913 ISSN:1672-7207
通讯作者:
Liu, Y.(liuyf@csu.edu.cn)
作者机构:
[彭东明; 王春燕; 刘艳飞; 苏小爱; 帅海涛; 盛毓; 李永欣] School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China;[刘艳飞] Key Laboratory of Resources Chemistry of Nonferrous Metals, Ministry of Education, Central South University, Changsha 410083, China;[彭东明] School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China
通讯机构:
School of Chemistry and Chemical Engineering, Central South University, China
关键词:
盐酸头孢吡肟;O-羧甲基壳聚糖;星点设计-效应面法;缓释微球
摘要:
以O-羧甲基壳聚糖(OCMC)为载体、戊二醛(GA)为交联剂,采用乳化交联法制备包覆盐酸头孢吡肟(CD)的药物缓释微球。以OCMC质量分数、CD与OCMC质量比(m(CD)/m(OCMC))、交联剂用量(V(GA)/m(OCMC))及油相乳化剂Span80质量分数为自变量,以载药量和包封率为因变量,采用星点设计-效应面法优化制备工艺,并对优化后的工艺进行验证。所得最优制备条件如下:OCMC质量分数为2.7%,m(CD)/m(OCMC)为0.665,交联剂用量V(GA)/m(OCMC)为8mL/g,Span80质量分数为4%;在最优工艺条件下制备的微球外观圆整,平均粒径为7μm,粒度跨距为1.52,载药量和包封率分别为(21.4±0.5)%和(42.3±0.7)%,缓释时间达到10d以上;星点设计-效应面法预测性良好。
语种:
中文
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Synthesis and structures of hypervalent organoantimony and organobismuth chlorides containing asymmetric C,E,C-chelating (E = O, S) ligands
作者:
Tan, Nianyuan;Chen, Yi
( 陈懿 ) ;Yin, Shuang-Feng
* ;Qiu, Renhua;Zhou, Yongbo;...
期刊:
Dalton Transactions ,2013年42(26):9476-9481 ISSN:1477-9226
通讯作者:
Yin, Shuang-Feng
作者机构:
[Chen, Yi; Tan, Nianyuan; Zhou, Yongbo; Yin, Shuang-Feng; Qiu, Renhua; Au, C. T.] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Tan, Nianyuan] Hunan Inst Engn, Coll Chem & Chem Engn, Xiangtan 411104, Peoples R China.;[Chen, Yi] Hunan Univ Chinese Med, Sch Basic Med, Changsha 410208, Hunan, Peoples R China.;[Au, C. T.] Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Hong Kong, Peoples R China.
通讯机构:
[Yin, Shuang-Feng] H;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
关键词:
Synthesis;structures;organobismuth
摘要:
Two asymmetric tridentate C,E,C-chelating ligand precursors, 1-Br-2-[(2′-BrC<inf>6</inf>H<inf>4</inf>CH<inf>2</inf>E)CH <inf>2</inf>]C<inf>10</inf>H<inf>6</inf> (E = O (1), E = S (2), were prepared in good yield. Lithiation of the two precursors was achieved by a reaction with n-BuLi, and was followed by treatment with SbCl<inf>3</inf> or BiCl<inf>3</inf> in a 1:1 molar ratio to give four air-stable hypervalent organoantimony and organobismuth chlorides with an asymmetric C,E,C-chelating ligand (E = O, S), i.e. (C<inf>6</inf>H<inf>4</inf>CH<inf>2</inf>OCH<inf>2</inf>C <inf>10</inf>H<inf>6</inf>)SbCl (3), (C<inf>6</inf>H<inf>4</inf>CH <inf>2</inf>SCH<inf>2</inf>C<inf>10</inf>H<inf>6</inf>)SbCl (4), (C <inf>6</inf>H<inf>4</inf>CH<inf>2</inf>OCH<inf>2</inf>C<inf>10</inf>H <inf>6</inf>)BiCl (5) and (C<inf>6</inf>H<inf>4</inf>CH<inf>2</inf>SCH <inf>2</inf>C<inf>10</inf>H<inf>6</inf>)BiCl (6). These compounds were characterized by NMR spectroscopy, elemental analysis and melting point determination. X-ray structure analysis of compounds 3-6 revealed that the donor atoms (O, S) are strongly coordinated to the metal atoms (Sb, Bi). Compounds 3-6 exhibit chirality and crystallize as racemic mixtures. This journal is ©The Royal Society of Chemistry 2013.
语种:
英文
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A mini-review on air-stable organometallic Lewis acids: synthesis, characterization, and catalytic application in organic synthesis
作者:
Qiu, Renhua
* ;Chen, Yi
( 陈懿 ) ;Yin, Shuang-Feng;Xu, Xinhua;Au, Chak-Tong
期刊:
RSC Advances ,2012年2(29):10774-10793 ISSN:2046-2069
通讯作者:
Qiu, Renhua
作者机构:
[Chen, Yi; Au, Chak-Tong; Yin, Shuang-Feng; Qiu, Renhua; Xu, Xinhua] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Chen, Yi] Hunan Univ Chinese Med, Coll Basic Med, Changsha 410208, Hunan, Peoples R China.;[Au, Chak-Tong] Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Hong Kong, Peoples R China.
通讯机构:
[Qiu, Renhua] H;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
摘要:
Organometallic Lewis acids play an important role in modern organic synthesis. How to design and synthesize highly efficient and recyclable organometallic Lewis acid catalysts that can be conveniently applied in chemical reactions are key issues for sustainable synthetic processes. In general, stronger acidity means higher catalytic activity for organometallic Lewis acids. However, with the rise in acidity, the compound becomes more susceptible to hydrolysis and cannot be recycled. Simultaneous improvement of the hygroscopic character and Lewis acidity/catalytic activity of organometallic Lewis acids is highly desirable from the standpoint of practical applications. In this mini-review, the history of air-stable organometallic Lewis acids is introduced, with emphasis on our research works on metallocene, organobismuth, and organoantimony Lewis acids to the aspects of synthesis, characterization and catalytic application in carbon–carbon bond (Friedel–Crafts acylation, Mukaiyama aldol reactions; allylation, cyclotrimerization, Mannich reactions, cross-condensation reactions) and carbon–heteroatom bond (acylation, S–S bond cleavage, glycosylation) formation reactions. In terms of stability, storage, versatile ability, high catalytic activity and chemo-/stereo-selectivity, the complexes will find broad applications in organic synthesis.
语种:
英文
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逐次最小二乘在高精度曲面建模方法(HASM)中的应用
作者:
陈曙;刘向前;彭力平
期刊:
武汉大学学报:信息科学版 ,2011年36(10):1246-1250 ISSN:1671-8860
通讯作者:
Wang, S.(wangsh.05b@igsnrr.ac.cn)
作者机构:
[陈曙] 韶关学院医学院;[刘向前] 湖南中医学院第一附属医院;[彭力平] 深圳市福田区中医院
通讯机构:
Institute of Geographic Science and Natural Resource Research, CAS, A11 Andingmenwai Street, China
关键词:
曲面建模;逐次最小二乘;空间插值
摘要:
从高精度曲面建模方法(HASM)曲面方程出发,结合数据平差理论,建立了基于独立单元的计算模型,并且采用逐次最小二乘法对计算方程进行分组求解。对HASM-AD和几种经典方法的精度进行了交叉验证,结果表明,该算法模拟误差的均方根差远小于其他空间插值方法。
语种:
中文
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双巷网络并行电法探测工作面内薄煤区范围
作者:
吴荣新;张平松;刘盛东
期刊:
岩石力学与工程学报 ,2009年28(9):1834-1838 ISSN:1000-6915
通讯作者:
Wu, R.(rxwu@aust.edu.cn)
作者机构:
[张平松; 吴荣新] School of Earth Science and Environmental Engineering, Anhui University of Science and Technology, Huainan, Anhui 232001, China;[刘盛东] School of Resources and Geosciences, China University of Mining and Technology, Xuzhou, Jiangsu 221008, China
通讯机构:
School of Earth Science and Environmental Engineering, Anhui University of Science and Technology, China
关键词:
采矿工程;网络并行电法;电阻率;煤层工作面;地质异常;薄煤区
摘要:
提出采用双巷网络并行电法探测工作面内薄煤区范围,为煤矿工作面内地质异常区探测提供新的物探手段,特别是对传统的无线电波透视难以穿透的大面宽工作面,更是有益的补充.网络并行电法采集数据方式简单,实现了整条电法测线电位数据的同步采集,显著提高了电法采集数据的效率和信噪比,可进行多种电极装置的电阻率反演.在工作面双巷中分别布置电法测线,所有电极均布置在巷帮煤壁中部腰线位置,利用网络并行电法仪采集电法数据,将双巷测站采集电法数据进行拼接,联合进行二维电阻率层析成像,可以得到工作面双巷间电阻率变化情况.根据薄煤区与正常煤体间的电性差异情况,分析薄煤区可能的电阻率表现形式,再结合已有地质资料,分析出工作面内的薄煤区及分布范围.淮南矿业集团张集矿综采工作面的探测及验证实例表明,该方法较好地查明了工作面内薄煤区的展布范围,有力地指导了工作面安全生产,并取得了显著的应用效果.
语种:
中文
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考虑剪胀性和应变软化的粉细砂双屈服面本构模型
作者:
杨琼梁;袁佳敏;黄星雨;颜冲;杨仁义;...
期刊:
水利学报 ,2008年39(2):129-136 ISSN:0559-9350
通讯作者:
Huang, M.-S.(mshuang@mail.tongji.edu.cn)
作者机构:
[杨琼梁; 袁佳敏; 黄星雨; 李博杨; 颜红] 湖南中医药大学药学院;[颜冲] 湖南中医药大学医学院;[杨仁义] 湖南中医药大学中西医结合学院;[苏啟后] 湖南中医药大学中医学院
通讯机构:
Tongji University, China
关键词:
粉细砂;剪胀性;应变软化;双屈服面
摘要:
针对上海粉细砂不存在唯一临界状态线的特点,对传统的砂土本构模型进行了改进,提出了一个能合理描述粉细砂剪胀性和应变软化特性的弹塑性本构模型。该模型采用双屈服面形式,可同时反映剪切变形及压缩变形机理。模型对传统修正剑桥模型中的剪胀性公式进行了改进,考虑了反映塑性体积应变由正转为负时对应的特征状态应力比与初始有效围压的相关性。为了描述应变软化特性,提出了一个利用残余状态应力比和峰值应力比的应变软化公式,可较为合理地反映粉细砂的应变软化特性。通过对上海粉细砂的多组试验结果模拟,验证了本文模型的合理性和有效性。
语种:
中文
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Automatic detection of Staphylococcus aureus and Shigella dysenteriae with separated electrodes series piezoelectric sensing technique
作者:
Zhu, Wenjie;Chen, Lingli
( 陈伶利 ) ;Yang, Zhihui;Chai, Liyuan
*
期刊:
World Journal of Microbiology and Biotechnology ,2008年24(7):1073-1079 ISSN:0959-3993
通讯作者:
Chai, Liyuan
作者机构:
[Zhu, Wenjie; Yang, Zhihui; Chai, Liyuan] Cent S Univ, Sch Met Sci & Engn, Changsha 410083, Hunan, Peoples R China.;[Chen, Lingli] Hunan Univ Tradit Chinese Med, Dept Basic Med, Changsha 410007, Hunan, Peoples R China.
通讯机构:
[Chai, Liyuan] C;Cent S Univ, Sch Met Sci & Engn, Changsha 410083, Hunan, Peoples R China.
关键词:
Automated;Detection;Series piezoelectric quartz crystal sensor;Shigella dysenteriae;Staphylococcus aureus
摘要:
The series piezoelectric quartz crystal (SPQC) sensing technique is a rapid and sensitive method for detection of microorganisms. In the present study, the detection device was composed of detecting system, signal generating system and data analyzing system. To magnify the amount of detection samples, eight independent SPQC sensors were parallel connected to form a muti-channel detecting unit. Electrodes were separated from the SPQC sensor and immersed into culture medium to detect the change of solution conductivity. The cell constant k was determined as 0.05 m, and the sensitivity interval of the device was from 550 to 600 μs. To maintain sensitivity of the SPQC sensor, a novel culture medium amino acid broth (AaB) was developed. It was nutrient with low conductivity and satisfied our detection device. For determining frequency detection time (FDT) expediently and accurately, FDT was defined afresh with fitting–differentiating method. Pathogens Staphylococcus aureus and Shigella dysenteriae were determined with an automated detecting device and the methods mentioned above. The calibration curves of FDT against density of bacteria showed a linear correlation coefficient (R ≥ 0.99) over the range of 10–106 cells ml−1. Detection results all fell inside the 95% confidence interval of a standard pour plate counting method. The reproducibility was also reviewed, and results showed that the device was stable and sensitive even after 180 days of employment.
语种:
英文
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二维快速子空间DOA估计算法
作者:
周日宝;方石林;常劲;罗友义
期刊:
华中科技大学学报(自然科学版) ,2008年36(4):20-23 ISSN:1671-4512
通讯作者:
Ma, H.(mahong@mail.hust.edu.cn)
作者机构:
[周日宝; 常劲; 罗友义] 湖南中医学院中药系;[方石林] 长沙市药品检验所
通讯机构:
Wuhan National Laboratory for Optoelectronics, Department of Electronics and Information Engineering, Huazhong University of Science and Technology, China
关键词:
阵列信号处理;DOA(波达方向)估计;快速子空间算法;谱峰搜索;计算复杂度
摘要:
提出一种二维快速子空间DOA估计算法,该算法利用阵列协方差矩阵的一个子矩阵得到降维的信号子空间,不需估计整个阵列的协方差矩阵,也不需进行特征值分解,从而使得该方法具有运算量小、复杂度低和易于实时处理的特点,因而可以应用在小数据样本和快速时变的信号环境中.理论分析和计算机仿真结果表明:与MUSIC算法相比,该算法运算量最多为MUSIC算法的1/4,低信噪比条件下DOA估计性能损失并不大,当信噪比大于5dB时,性能与MUSIC算法相当.
语种:
中文
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探测器交会小天体的UPF自主导航方案
作者:
董克芳;田心义;孙绍裘;朱付平;周昭辉;...
期刊:
吉林大学学报(工学版) ,2007年37(3):691-695 ISSN:1671-5497
通讯作者:
Li, P.(lipeng@astro.hit.edu.cn)
作者机构:
[董克芳; 田心义; 孙绍裘; 周昭辉; 孙达武] 湖南中医学院第二附属医院骨科;[朱付平; 肖符武] 湖南中医学院第一附属医院骨科
通讯机构:
Deep Space Exploration Research Center, Harbin Institute of Technology, China
关键词:
飞行器控制与导航技术;自主光学导航;Unscented粒子滤波;非线性非高斯;自主轨道确定
摘要:
采用基于目标天体信息的光学导航方案实现探测器交会小天体,利用导航相机提高了定轨精度和星历精度。在此基础上,针对深空导航的轨道确定过程中可能存在的初始估计信息误差较大、状态及量测误差不服从高斯分布等问题,将UPF(Unscented Particle Filter)引入到导航方案中。该方案利用UPF在处理非线性非高斯问题上的优势,克服了EKF、UKF、PF等传统滤波方案对非线性非高斯状态模型、量测模型的近似处理所带来的影响。数值仿真表明了该方案的可行性。
语种:
中文
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Immunomagnetic separation and MS/SPR end-detection combined procedure for rapid detection of Staphylococcus aureus and protein A
作者:
Chen, Lingli
( 陈伶利 ) ;Deng, Le
* ;Liu, Linlin;Peng, Zhihui
期刊:
Biosensors and Bioelectronics ,2007年22(7):1487-1492 ISSN:0956-5663
通讯作者:
Deng, Le
作者机构:
[Chen, Lingli; Deng, Le; Liu, Linlin; Peng, Zhihui] Hunan Normal Univ, Dept Microbiol, Changsha 410081, Hunan, Peoples R China.
通讯机构:
[Deng, Le] H;Hunan Normal Univ, Dept Microbiol, Changsha 410081, Hunan, Peoples R China.
关键词:
S. aureus;SPA;IMS-MS/SPR
摘要:
The aim of this study was to establish an IMS-MS/SPR technique for the detection of Staphylococcus aureus (S. aureus) and Staphylococcus protein A (SPA) at the same time, which consists of isolating S. aureus and trapping-enrichmenting its SPA by IMS, and the end point is determined by using either MS or SPR measurements. Magnetic bead (MB) containing aldehyde group was synthesized with latex-polymerization and immunomagnetic bead (IMB) was fabricated by modifying its surface with an oriented layer of human IgG in covalent linkage. As soon as sample of pulverator-treated bacterial cell lysate (10<sup>8</sup> cfu/mL) was incubated with IMB at 4 °C for 30 min, SPA was captured and separated from the mixed solution in a few minutes by the IMB and then detected with mass spectrometry after washing. SPR was used to detect S. aureus quantitatively in situ at the end-detection procedure. All in all, this technique can be employed to detect rapidly SPA and S. aureus within 2 h and also be applied to detect other cells or their membrane proteins with changed modified antibodies. ©2006 Elsevier B.V. All rights reserved.
语种:
英文
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反相高效液相色谱法测定大鼠血浆中补阳还五汤中黄芪甲苷的含量及药动学研究
作者:
贺福元;刘平安;邓凯文;刘文龙;邓常青
期刊:
中国药学杂志 ,2005年40(21):1647-1649 ISSN:1001-2494
通讯作者:
He, F.-Y.
作者机构:
[刘平安; 贺福元; 刘文龙] Department of Pharmaceutics, Hunan College of TCM, Changsha 410007, China;[贺福元] School of Pharmacy, Chengdu University of TCM, Chengdu 610075, China;[邓常青] Institute of TCM's Diagnosis, Hunan College of TCM, Changsha 410007, China;[邓凯文] First Affiliated Hospital, Hunan College of TCM, Changsha 410007, China
通讯机构:
[He, F.-Y.] D;Department of Pharmaceutics, Hunan College of TCM, China
关键词:
补阳还五汤;总苷;黄芪甲苷;反相高效液相色谱法;药动学
摘要:
目的对大鼠血浆补阳还五汤中黄芪甲苷的含量进行了测定,并进行了药动学研究.方法采用反相高效液相色谱法测定,其条件为色谱柱:C18柱(4.6 mm×250 mm,5 μm);检测波长:203nm;流动相:乙腈-水(35:65),流速:1 mL·min-1;柱压:(15.17±0.4137)MPa;温度:45℃.药动学参数采用3P87软件处理.结果血浆样品中黄芪甲苷线性范围为2.4~12μg,r=0.999 6,回收率为100.2%,RSD为1.100%.黄芪甲苷药动学参数:复方:t1/2α为1.392 h;t1/2β为14.24h;K12为0.162 4 h-1;K21为0.304 5 h-1;K10为0.079 58 h-1;总苷:t1/2α为2.641 h;t1/2β为37.12 h;K12为0.1283 h-1;K21为0.1071 h-1;K10为0.045 74h-1.结论该法快速、简便,准确.补阳还五汤复方及总苷中黄芪甲苷药动学为二室模型.中药复方成分组合对药动学参数有一定影响.
语种:
中文
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HPLC-MS/ESI测定人血浆中米格列醇
作者:
王峰;周晋;向大雄;肖轶雯;原海燕;...
期刊:
中国药学杂志 ,2005年40(1):51-53 ISSN:1001-2494
通讯作者:
Wang, F.
作者机构:
[李焕德; 王峰; 向大雄; 周志凌; 肖轶雯; 原海燕; 徐萍] Clinical Pharmacy Laboratory, Second Xiangya Hospital, Central South University, Changsha 410011, China;[周晋] Hunan Coll. Traditional Chinese M., Changsha 410007, China
通讯机构:
[Wang, F.] C;Clinical Pharmacy Laboratory, Second Xiangya Hospital, Central South University, China
会议名称:
中国药学会学术年会
会议时间:
2004-07-19
会议地点:
昆明
会议论文集名称:
2004年中国药学会学术年会论文集
关键词:
高效液相色谱-质谱;米格列醇;药动学
摘要:
目的建立高效液相色谱-质谱联用测定人血浆中米格列醇浓度的方法,并用于健康人体内米格列醇药物动力学研究.方法采用MACHEREY-NAGELCN色谱柱(4.6mm×250mm,5μm),柱温50℃,以乙腈-甲醇-0.02%盐酸水溶液为流动相,流速0.80mL·min-1;质谱采用电喷雾电离源正源(ESI+),定量分析采用选择离子监测(SIR),质荷比为准分子离子峰;血浆样品用乙腈沉淀后,将乙腈挥干,残渣用氯仿-异丙醇提取杂质,磷酸液提取药物,外标法定量.结果米格列醇浓度在32~3 200 ng·mL-1内线性关系良好,相关系数为0.999 7,最低检测浓度5ng·mL-1(S/N=3).批内、批间RSD符合方法学要求,单次服用100mg米格列醇片后药动学参数AUC0~12,AUC0~∞,cmax,tmax,t1/2分别为(7 592.7±4 207.4)ng·h·mL-1,(7 957.6±4 425.7)ng·h·mL-1,(1 883.9±912.3)ng·mL-1,(2.4±0.6)h,(2.5±0.9)h.结论该方法稳定、灵敏度高、操作简单,适用于米格列醇血药浓度测定及药动学研究.
语种:
中文
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葛根总黄酮生物黏附性缓释片在家犬体内的药动学及生物利用度研究
作者:
向大雄;李焕德;罗杰英;袁红波;张杰;...
期刊:
中国药学杂志 ,2005年40(7):532-534 ISSN:1001-2494
通讯作者:
Xiang, D.-X.(xiangdaxiong@netease.com)
作者机构:
[向大雄; 李焕德; 龚莉; 袁红波; 孙银香] Second Xiangya Hospital, Central Southern University, Changsha, 410011, China;[张杰] Pharmacy Department of Third Xangya Hospital, Central Southern University, Changsha 410013, China;[罗杰英] Hunan College of the Traditional Chinese Medicine, Changsha 410007, China
通讯机构:
[Xiang, D.-X.] S;Second Xiangya Hospital, Central Southern University, China
关键词:
葛根总黄酮;生物黏附;缓释片;药动学;生物利用度
摘要:
目的 研究葛根总黄酮(TPF)生物黏附性缓释片和普通片在家犬体内的药动学与生物利用度,揭示葛根总黄酮生物黏附性缓释片在家犬体内的药动学变化规律,为临床合理运用该药提供实验依据?方法健康家犬6只,以自身前后交叉对照方式,单剂量分别给予葛根总黄酮生物黏附性缓释片和普通片600mg。葛根素血药浓度经时数据以3P87药动学程序处理,以对两种片剂在家犬体内的动态过程进行拟合,以非室模型统计矩方法,计算药时曲线下面积(AUC0→2),并由血药浓度数据读取Cmax,tmax,经统计分析比较普通片与缓释片的生物利用度。结果单剂量给予家犬TPF片及生物黏附片后,葛根素血药浓度变化符合一级吸收一室模型,Ke分别为(0.461±0.115)h^-1,(0.238±0.042)h^-1;Ka分别为(0.844±0.404)h^-1,(0.446±0.100)h^-1;t1/2ka分别为(0.954±0.352)h,(1.609±0.292)h,t1/2ke分别为(1.570±0.333)h,(2.995±0.617)h。普通片的AUC0-12,Cmax,tmax分别为(4430±998)ng·h·mL^-1,(1241±247)ng·mL^-1和(2.2±0.6)h。缓释片的AUC0→12,cmax和tmax分别为(6459±738)ng·h·mL^-1,(1040±134)ng·mL^-1和(4.0±0.7)h。以普通片为参比制剂,缓释片的生物利用度为(151.4±37.6)%。结论葛根总黄酮缓释片在家犬体内表现出较好的缓释特性,主要药动学参数发生了变化,生物黏附片的生物利用度也明显高与普通片。
语种:
中文
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