Combat biofilm by bacteriostatic aptamer-functionalized graphene oxide
作者:
Mao, Biyao;Cheng, Lijuan
( 程莉娟 ) ;Wang, Sheng;Zhou, Jiaqi;Deng, Le
*
期刊:
Biotechnology and Applied Biochemistry ,2018年65(3):355-361 ISSN:0885-4513
通讯作者:
Deng, Le
作者机构:
[Cheng, Lijuan; Zhou, Jiaqi; Mao, Biyao; Deng, Le; Wang, Sheng] Hunan Normal Univ, State Key Lab Dev Biol Freshwater Fish, Coll Life Sci, Changsha, Hunan, Peoples R China.;[Cheng, Lijuan] Hunan Univ Chinese Med, Dept Biochem & Mol Biol, Changsha, Hunan, Peoples R China.
通讯机构:
[Deng, Le] H;Hunan Normal Univ, State Key Lab Dev Biol Freshwater Fish, Coll Life Sci, Changsha, Hunan, Peoples R China.
关键词:
biofilm;conjugate;Salmonella typhimurium;target delivery
摘要:
Biofilms are the main reason for a large number deaths and high health costs. Their better protection compared to planktonic form against conventional antibiotics leads to poor treatment efficiency. Nanoagent-targeted delivery is a promising avenue for disease therapeutic, but its application targeting biofilms has not been reported currently. The roles, if any, of aptamers acting as delivery carrier and targeting factor, the graphene oxide (GO), and GO modified with aptamers against biofilms were then systematically evaluated. Here, we successfully developed an aptamer-targeted GO strategy against biofilms. We investigated the efficacy of aptamer–GO conjugates by UV spectrophotometer, inverted microscopy, and atomic force microscopy; 93.5 ± 3.4% Salmonella typhimurium biofilms were inhibited and 84.6 ± 5.1% of biofilms were dispersed by a ST-3-GO conjugate. More importantly, this conjugate represented distinctively toxicity to S. typhimurium. Thus, this strategy significantly displays excellent antibiofilm properties and may serve as a long-term solution for biofilm control. © 2018 International Union of Biochemistry and Molecular Biology, Inc.
语种:
英文
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非那雄胺/聚碳酸亚丙酯马来酸酯缓释微球的制备与性能
作者:
彭东明;张航;王晓红;刘艳飞
期刊:
中南大学学报(自然科学版) ,2014年45(7):2171-2175 ISSN:1672-7207
通讯作者:
Liu, Yanfei
作者机构:
[彭东明; 刘艳飞; 王晓红; 张航] School of Chemistry and Chemical Engineering, Central South University, Changsha, China;[彭东明] School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China
通讯机构:
School of Chemistry and Chemical Engineering, Central South University, Changsha, China
关键词:
非那雄胺;聚碳酸亚丙酯马来酸酯;缓释微球;药物传递体系
摘要:
以新型聚合物聚碳酸亚丙酯马来酸酯(PPCM)为载体,采用Oil/Water单乳液溶剂挥发法制备药物非那雄胺(finasteride)的缓释微球,并研究聚合物PPCM与药物finasteride的质量比对微球特性的影响。研究结果表明:所得PPCM微球外观圆整,平均粒径约为2 μm。随着非那雄胺比例的增加,微球的载药量提高,而药物的包封率则明显降低。在m(PPCM):m(finasteride)为5:1的条件下,获得较高的载药量和包封率,分别为14.78%和66.17%。在pH 7.4的磷酸盐缓冲溶液中,载药微球的体外释放时间达42 d,药物累积释放量为(92.59±2.62)%。微球的释药特性符合Higuchi方程Q_t=3.11+15.07 t~(1/2)。PPCM适用于长效缓释药物传递系统。
语种:
中文
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Analysis of the Interaction of Tarantula Toxin Jingzhaotoxin-III (beta-TRTX-Cj1 alpha) with the Voltage Sensor of Kv2.1 Uncovers the Molecular Basis for Cross-Activities on Kv2.1 and Nav1.5 Channels
作者:
Tao, Huai
( 陶怀 ) ;Chen, Jin J.;Xiao, Yu C.;Wu, Yuan Y.;Su, Hai B.;...
期刊:
Biochemistry ,2013年52(42):7439-7448 ISSN:0006-2960
通讯作者:
Liang, Song P.
作者机构:
[Tao, Huai; Wang, Heng Y.; Li, Dan; Liang, Song P.; Liu, Zhong H.; Wu, Yuan Y.; Su, Hai B.; Xiao, Yu C.; Wang, Mei C.] Hunan Normal Univ, Coll Life Sci, Key Lab Prot Chem & Dev Biol, Minist Educ, Changsha 410081, Hunan, Peoples R China.;[Tao, Huai] Hunan Univ Chinese Med, Dept Biochem & Mol Biol, Changsha 410208, Hunan, Peoples R China.;[Chen, Jin J.] Hunan Agr Univ, Coll Biol Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Deng, Mel C.] Cent S Univ, Sch Biol Sci & Technol, Dept Biochem, Changsha 410013, Hunan, Peoples R China.
通讯机构:
[Liang, Song P.] H;Hunan Normal Univ, Coll Life Sci, Key Lab Prot Chem & Dev Biol, Minist Educ, Changsha 410081, Hunan, Peoples R China.
摘要:
Animal venoms contain a fascinating array of divergent peptide toxins that have cross-activities on different types of voltage-gated ion channels. However, the underlying mechanism remains poorly understood. Jingzhaotoxin-III (JZTX-III), a 36-residue peptide from the tarantula Chilobrachys jingzhao, is specific for Nav1.5 and Kv2.1 channels over the majority of other ion channel subtypes. JZTX-III traps the Nav1.5 DII voltage sensor at closed state by binding to the DIIS3-S4 linker. In this study, electrophysiological experiments showed that JZTX-III had no effect on five voltage-gated potassium channel subtypes (Kv1.4, Kv3.1, and Kv4.1-4.3), whereas it significantly inhibited Kv2.1 with an IC<inf>50</inf> of 0.71 ±0.01 μM. Mutagenesis and modeling data suggested that JZTX-III docks at the Kv2.1 voltage-sensor paddle. Alanine replacement of Phe274, Lys280, Ser281, Leu283, Gln284, and Val288 could decrease JZTX-III affinity by 7-, 9-, 34-, 12-, 9-, and 7-fold, respectively. Among them, S281 is the most crucial determinant, and the substitution with Thr only slightly reduced toxin sensitivity. In contrast, a single conversion of Ser281 to Ala, Phe, Ile, Val, or Glu increased the IC<inf>50</inf> value by >34-fold. Alanine-scanning mutagenesis experiments indicated that the functional surface of JZTX-III bound to the Kv2.1 channel is composed of four hydrophobic residues (Trp8, Trp28, Trp30, and Val33) and three charged residues (Arg13, Lys15, and Glu34). The bioactive surfaces of JZTX-III interacting with Kv2.1 and Nav1.5 are only partially overlapping. These results strongly supported the hypothesis that animal toxins might use partially overlapping bioactive surfaces to target the voltage-sensor paddles of two different types of ion channels. Increasing our understanding of the molecular mechanisms of toxins interacting with voltage-gated sodium and potassium channels may provide new molecular insights into the design of more potent ion channel inhibitors. ©2013 American Chemical Society.
语种:
英文
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盐酸头孢吡肟缓释微球的制备与工艺优化
作者:
彭东明;王春燕;刘艳飞;李永欣;苏小爱;...
期刊:
中南大学学报(自然科学版) ,2013年44(3):907-913 ISSN:1672-7207
通讯作者:
Liu, Y.(liuyf@csu.edu.cn)
作者机构:
[彭东明; 王春燕; 刘艳飞; 苏小爱; 帅海涛; 盛毓; 李永欣] School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China;[刘艳飞] Key Laboratory of Resources Chemistry of Nonferrous Metals, Ministry of Education, Central South University, Changsha 410083, China;[彭东明] School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China
通讯机构:
School of Chemistry and Chemical Engineering, Central South University, China
关键词:
盐酸头孢吡肟;O-羧甲基壳聚糖;星点设计-效应面法;缓释微球
摘要:
以O-羧甲基壳聚糖(OCMC)为载体、戊二醛(GA)为交联剂,采用乳化交联法制备包覆盐酸头孢吡肟(CD)的药物缓释微球。以OCMC质量分数、CD与OCMC质量比(m(CD)/m(OCMC))、交联剂用量(V(GA)/m(OCMC))及油相乳化剂Span80质量分数为自变量,以载药量和包封率为因变量,采用星点设计-效应面法优化制备工艺,并对优化后的工艺进行验证。所得最优制备条件如下:OCMC质量分数为2.7%,m(CD)/m(OCMC)为0.665,交联剂用量V(GA)/m(OCMC)为8mL/g,Span80质量分数为4%;在最优工艺条件下制备的微球外观圆整,平均粒径为7μm,粒度跨距为1.52,载药量和包封率分别为(21.4±0.5)%和(42.3±0.7)%,缓释时间达到10d以上;星点设计-效应面法预测性良好。
语种:
中文
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Synthesis and structures of hypervalent organoantimony and organobismuth chlorides containing asymmetric C,E,C-chelating (E = O, S) ligands
作者:
Tan, Nianyuan;Chen, Yi
( 陈懿 ) ;Yin, Shuang-Feng
* ;Qiu, Renhua;Zhou, Yongbo;...
期刊:
Dalton Transactions ,2013年42(26):9476-9481 ISSN:1477-9226
通讯作者:
Yin, Shuang-Feng
作者机构:
[Chen, Yi; Tan, Nianyuan; Zhou, Yongbo; Yin, Shuang-Feng; Qiu, Renhua; Au, C. T.] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Tan, Nianyuan] Hunan Inst Engn, Coll Chem & Chem Engn, Xiangtan 411104, Peoples R China.;[Chen, Yi] Hunan Univ Chinese Med, Sch Basic Med, Changsha 410208, Hunan, Peoples R China.;[Au, C. T.] Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Hong Kong, Peoples R China.
通讯机构:
[Yin, Shuang-Feng] H;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
关键词:
Synthesis;structures;organobismuth
摘要:
Two asymmetric tridentate C,E,C-chelating ligand precursors, 1-Br-2-[(2′-BrC<inf>6</inf>H<inf>4</inf>CH<inf>2</inf>E)CH <inf>2</inf>]C<inf>10</inf>H<inf>6</inf> (E = O (1), E = S (2), were prepared in good yield. Lithiation of the two precursors was achieved by a reaction with n-BuLi, and was followed by treatment with SbCl<inf>3</inf> or BiCl<inf>3</inf> in a 1:1 molar ratio to give four air-stable hypervalent organoantimony and organobismuth chlorides with an asymmetric C,E,C-chelating ligand (E = O, S), i.e. (C<inf>6</inf>H<inf>4</inf>CH<inf>2</inf>OCH<inf>2</inf>C <inf>10</inf>H<inf>6</inf>)SbCl (3), (C<inf>6</inf>H<inf>4</inf>CH <inf>2</inf>SCH<inf>2</inf>C<inf>10</inf>H<inf>6</inf>)SbCl (4), (C <inf>6</inf>H<inf>4</inf>CH<inf>2</inf>OCH<inf>2</inf>C<inf>10</inf>H <inf>6</inf>)BiCl (5) and (C<inf>6</inf>H<inf>4</inf>CH<inf>2</inf>SCH <inf>2</inf>C<inf>10</inf>H<inf>6</inf>)BiCl (6). These compounds were characterized by NMR spectroscopy, elemental analysis and melting point determination. X-ray structure analysis of compounds 3-6 revealed that the donor atoms (O, S) are strongly coordinated to the metal atoms (Sb, Bi). Compounds 3-6 exhibit chirality and crystallize as racemic mixtures. This journal is ©The Royal Society of Chemistry 2013.
语种:
英文
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Discrimination of A1555G and C1494T Point Mutations in the Mitochondrial 12S rRNA Gene by On/Off Switch
作者:
Guo, Zi-Fen;Guo, Wu-Shuang;Xiao, Li;Gao, Guo-Qiang;Lan, Fen;...
期刊:
Molecular Biotechnology ,2012年166(1):234-242 ISSN:1073-6085
通讯作者:
Li, Kai
作者机构:
[Guo, Zi-Fen; Lan, Fen; Gao, Guo-Qiang; Guo, Wu-Shuang; Liao, Duan-Fang] Univ S China, Inst Pharm & Pharmacol, Hengyang 421001, Hunan, Peoples R China.;[Li, Kai] Soochow Univ, Coll Med, Dept Pharmacol, Suzhou 215123, Jiangsu, Peoples R China.;[Lu, Xue-Guan; Xiao, Li] Soochow Univ, Affiliated Hosp 2, Mol Med Ctr, Suzhou 215004, Jiangsu, Peoples R China.;[Liao, Duan-Fang] Hunan Univ Chinese Med, Sch Pharm, Dept Tradit Chinese Diognot, Changsha 410208, Hunan, Peoples R China.
通讯机构:
[Li, Kai] S;Soochow Univ, Coll Med, Dept Pharmacol, Suzhou 215123, Jiangsu, Peoples R China.
关键词:
Mitochondrial 12S rRNA;Exo(+) polymerase;Phosphorothioate modification;Aminoglycoside antibiotics
摘要:
The objective of this study was to apply the "on/off" switch consisting of 3' phosphorothioate-modified allele specific primers and exo(+) polymerase in single base discrimination of A1555G and C1494T mutations in the highly conserved sites of the mitochondrial 12S rRNA. The two point mutations are the hotspot mutations associated with either aminoglycoside antibiotics induced deafness or inherited nonsyndromic hearing loss. The PCR products of mitochondrial DNA (mtDNA) 12S rRNA gene were inserted into the pMD19-T vector for transformation into Escherichia coli JM109 competent cells for preparing wild-type pMD19-T/mt vector. Inverse PCR was carried out for mtDNA 12S rRNA gene C1494T and A1555G mutagenesis and DpnI endonuclease degradating methylated pMD19-T/mt vector existing in the inverse PCR products was carried out to construct the mutation-type pMD19-T/mtM vector. These constructed vectors were confirmed by DNA sequencing. Allelic specific primers targeting wild-type and mutation-type templates were designed with 3' terminal phosphorothioate modification. Two-directional primer extension was performed using Pfu polymerases. Amplified by exo(+) polymerase, allelic specific primers perfectly matching wild-type allele were extended while no products were produced from primers targeting point-mutated deafness-related allele. Similarly, allelic specific primers perfectly matching point-mutated deafness-related mutation-type allele were extended and no products were yielded from primers targeting wild-type allele. No specific product was observed in the primer extension reaction mediated by on/off switch in screening the mtDNA 12S rRNA gene harboring either C1494T or A1555G mutation in 40 healthy volunteers tested. These data suggest that the "off switch" mediated by exo(+) polymerase is highly reliable in the diagnosis of monogenic diseases and the novel "on/off" switch has enormous applications in systematic and extended screening of the12S rRNA gene A1555G and C1494T mutations. The established assay can be widely used not only for hearing loss patients but also for normal subjects before the use of aminoglycoside antibiotics.
语种:
英文
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逐次最小二乘在高精度曲面建模方法(HASM)中的应用
作者:
陈曙;刘向前;彭力平
期刊:
武汉大学学报:信息科学版 ,2011年36(10):1246-1250 ISSN:1671-8860
通讯作者:
Wang, S.(wangsh.05b@igsnrr.ac.cn)
作者机构:
[陈曙] 韶关学院医学院;[刘向前] 湖南中医学院第一附属医院;[彭力平] 深圳市福田区中医院
通讯机构:
Institute of Geographic Science and Natural Resource Research, CAS, A11 Andingmenwai Street, China
关键词:
曲面建模;逐次最小二乘;空间插值
摘要:
从高精度曲面建模方法(HASM)曲面方程出发,结合数据平差理论,建立了基于独立单元的计算模型,并且采用逐次最小二乘法对计算方程进行分组求解。对HASM-AD和几种经典方法的精度进行了交叉验证,结果表明,该算法模拟误差的均方根差远小于其他空间插值方法。
语种:
中文
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双巷网络并行电法探测工作面内薄煤区范围
作者:
吴荣新;张平松;刘盛东
期刊:
岩石力学与工程学报 ,2009年28(9):1834-1838 ISSN:1000-6915
通讯作者:
Wu, R.(rxwu@aust.edu.cn)
作者机构:
[张平松; 吴荣新] School of Earth Science and Environmental Engineering, Anhui University of Science and Technology, Huainan, Anhui 232001, China;[刘盛东] School of Resources and Geosciences, China University of Mining and Technology, Xuzhou, Jiangsu 221008, China
通讯机构:
School of Earth Science and Environmental Engineering, Anhui University of Science and Technology, China
关键词:
采矿工程;网络并行电法;电阻率;煤层工作面;地质异常;薄煤区
摘要:
提出采用双巷网络并行电法探测工作面内薄煤区范围,为煤矿工作面内地质异常区探测提供新的物探手段,特别是对传统的无线电波透视难以穿透的大面宽工作面,更是有益的补充.网络并行电法采集数据方式简单,实现了整条电法测线电位数据的同步采集,显著提高了电法采集数据的效率和信噪比,可进行多种电极装置的电阻率反演.在工作面双巷中分别布置电法测线,所有电极均布置在巷帮煤壁中部腰线位置,利用网络并行电法仪采集电法数据,将双巷测站采集电法数据进行拼接,联合进行二维电阻率层析成像,可以得到工作面双巷间电阻率变化情况.根据薄煤区与正常煤体间的电性差异情况,分析薄煤区可能的电阻率表现形式,再结合已有地质资料,分析出工作面内的薄煤区及分布范围.淮南矿业集团张集矿综采工作面的探测及验证实例表明,该方法较好地查明了工作面内薄煤区的展布范围,有力地指导了工作面安全生产,并取得了显著的应用效果.
语种:
中文
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考虑剪胀性和应变软化的粉细砂双屈服面本构模型
作者:
杨琼梁;袁佳敏;黄星雨;颜冲;杨仁义;...
期刊:
水利学报 ,2008年39(2):129-136 ISSN:0559-9350
通讯作者:
Huang, M.-S.(mshuang@mail.tongji.edu.cn)
作者机构:
[杨琼梁; 袁佳敏; 黄星雨; 李博杨; 颜红] 湖南中医药大学药学院;[颜冲] 湖南中医药大学医学院;[杨仁义] 湖南中医药大学中西医结合学院;[苏啟后] 湖南中医药大学中医学院
通讯机构:
Tongji University, China
关键词:
粉细砂;剪胀性;应变软化;双屈服面
摘要:
针对上海粉细砂不存在唯一临界状态线的特点,对传统的砂土本构模型进行了改进,提出了一个能合理描述粉细砂剪胀性和应变软化特性的弹塑性本构模型。该模型采用双屈服面形式,可同时反映剪切变形及压缩变形机理。模型对传统修正剑桥模型中的剪胀性公式进行了改进,考虑了反映塑性体积应变由正转为负时对应的特征状态应力比与初始有效围压的相关性。为了描述应变软化特性,提出了一个利用残余状态应力比和峰值应力比的应变软化公式,可较为合理地反映粉细砂的应变软化特性。通过对上海粉细砂的多组试验结果模拟,验证了本文模型的合理性和有效性。
语种:
中文
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二维快速子空间DOA估计算法
作者:
周日宝;方石林;常劲;罗友义
期刊:
华中科技大学学报(自然科学版) ,2008年36(4):20-23 ISSN:1671-4512
通讯作者:
Ma, H.(mahong@mail.hust.edu.cn)
作者机构:
[周日宝; 常劲; 罗友义] 湖南中医学院中药系;[方石林] 长沙市药品检验所
通讯机构:
Wuhan National Laboratory for Optoelectronics, Department of Electronics and Information Engineering, Huazhong University of Science and Technology, China
关键词:
阵列信号处理;DOA(波达方向)估计;快速子空间算法;谱峰搜索;计算复杂度
摘要:
提出一种二维快速子空间DOA估计算法,该算法利用阵列协方差矩阵的一个子矩阵得到降维的信号子空间,不需估计整个阵列的协方差矩阵,也不需进行特征值分解,从而使得该方法具有运算量小、复杂度低和易于实时处理的特点,因而可以应用在小数据样本和快速时变的信号环境中.理论分析和计算机仿真结果表明:与MUSIC算法相比,该算法运算量最多为MUSIC算法的1/4,低信噪比条件下DOA估计性能损失并不大,当信噪比大于5dB时,性能与MUSIC算法相当.
语种:
中文
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探测器交会小天体的UPF自主导航方案
作者:
董克芳;田心义;孙绍裘;朱付平;周昭辉;...
期刊:
吉林大学学报(工学版) ,2007年37(3):691-695 ISSN:1671-5497
通讯作者:
Li, P.(lipeng@astro.hit.edu.cn)
作者机构:
[董克芳; 田心义; 孙绍裘; 周昭辉; 孙达武] 湖南中医学院第二附属医院骨科;[朱付平; 肖符武] 湖南中医学院第一附属医院骨科
通讯机构:
Deep Space Exploration Research Center, Harbin Institute of Technology, China
关键词:
飞行器控制与导航技术;自主光学导航;Unscented粒子滤波;非线性非高斯;自主轨道确定
摘要:
采用基于目标天体信息的光学导航方案实现探测器交会小天体,利用导航相机提高了定轨精度和星历精度。在此基础上,针对深空导航的轨道确定过程中可能存在的初始估计信息误差较大、状态及量测误差不服从高斯分布等问题,将UPF(Unscented Particle Filter)引入到导航方案中。该方案利用UPF在处理非线性非高斯问题上的优势,克服了EKF、UKF、PF等传统滤波方案对非线性非高斯状态模型、量测模型的近似处理所带来的影响。数值仿真表明了该方案的可行性。
语种:
中文
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基于贝叶斯网络的中医辨证系统
作者:
朱咏华;朱文锋
期刊:
湖南大学学报. 自然科学版 ,2006年33(4):123-125 ISSN:1000-2472
通讯作者:
Zhu, Y.-H.
作者机构:
[朱咏华] Institute of Life Science and Technology, Hunan Univ., Changsha 410082, China;[朱文锋] Institute of Traditional Chinese Medicine Diagnosis, Hunan Univ. of Traditional Chinese Medicine, Changsha 410007, China
通讯机构:
Institute of Life Science and Technology, Hunan Univ., China
关键词:
医学计算;贝叶斯网络;贝叶斯概率;证素;辨证系统;思维规律
摘要:
将贝叶斯网络运用于中医辨证系统的研究,以更加量化中医辨证诊断系统.通过将中医体系中的916个证候,51项证素及其构成的1700条证名构成中医辨证贝叶斯网络的节点集,初步建立起中医辨证数据库并通过网络学习,形成中医辨证贝叶斯网络结构及概率表.利用建立的贝叶斯网络中医辨证系统,进行数据计量分析、推理验证证候-证素-证名间的关系,其结果与中医专家经验有很高的吻合性,尽管其仍未能全面反映中医辨证的思维能力.所以贝叶斯网络是对中医辨证进行信息挖掘处理的一种较好方法,可应用于中医人工智能辨证系统的建立.
语种:
中文
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反相高效液相色谱法测定大鼠血浆中补阳还五汤中黄芪甲苷的含量及药动学研究
作者:
贺福元;刘平安;邓凯文;刘文龙;邓常青
期刊:
中国药学杂志 ,2005年40(21):1647-1649 ISSN:1001-2494
通讯作者:
He, F.-Y.
作者机构:
[刘平安; 贺福元; 刘文龙] Department of Pharmaceutics, Hunan College of TCM, Changsha 410007, China;[贺福元] School of Pharmacy, Chengdu University of TCM, Chengdu 610075, China;[邓常青] Institute of TCM's Diagnosis, Hunan College of TCM, Changsha 410007, China;[邓凯文] First Affiliated Hospital, Hunan College of TCM, Changsha 410007, China
通讯机构:
[He, F.-Y.] D;Department of Pharmaceutics, Hunan College of TCM, China
关键词:
补阳还五汤;总苷;黄芪甲苷;反相高效液相色谱法;药动学
摘要:
目的对大鼠血浆补阳还五汤中黄芪甲苷的含量进行了测定,并进行了药动学研究.方法采用反相高效液相色谱法测定,其条件为色谱柱:C18柱(4.6 mm×250 mm,5 μm);检测波长:203nm;流动相:乙腈-水(35:65),流速:1 mL·min-1;柱压:(15.17±0.4137)MPa;温度:45℃.药动学参数采用3P87软件处理.结果血浆样品中黄芪甲苷线性范围为2.4~12μg,r=0.999 6,回收率为100.2%,RSD为1.100%.黄芪甲苷药动学参数:复方:t1/2α为1.392 h;t1/2β为14.24h;K12为0.162 4 h-1;K21为0.304 5 h-1;K10为0.079 58 h-1;总苷:t1/2α为2.641 h;t1/2β为37.12 h;K12为0.1283 h-1;K21为0.1071 h-1;K10为0.045 74h-1.结论该法快速、简便,准确.补阳还五汤复方及总苷中黄芪甲苷药动学为二室模型.中药复方成分组合对药动学参数有一定影响.
语种:
中文
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HPLC-MS/ESI测定人血浆中米格列醇
作者:
王峰;周晋;向大雄;肖轶雯;原海燕;...
期刊:
中国药学杂志 ,2005年40(1):51-53 ISSN:1001-2494
通讯作者:
Wang, F.
作者机构:
[李焕德; 王峰; 向大雄; 周志凌; 肖轶雯; 原海燕; 徐萍] Clinical Pharmacy Laboratory, Second Xiangya Hospital, Central South University, Changsha 410011, China;[周晋] Hunan Coll. Traditional Chinese M., Changsha 410007, China
通讯机构:
[Wang, F.] C;Clinical Pharmacy Laboratory, Second Xiangya Hospital, Central South University, China
会议名称:
中国药学会学术年会
会议时间:
2004-07-19
会议地点:
昆明
会议论文集名称:
2004年中国药学会学术年会论文集
关键词:
高效液相色谱-质谱;米格列醇;药动学
摘要:
目的建立高效液相色谱-质谱联用测定人血浆中米格列醇浓度的方法,并用于健康人体内米格列醇药物动力学研究.方法采用MACHEREY-NAGELCN色谱柱(4.6mm×250mm,5μm),柱温50℃,以乙腈-甲醇-0.02%盐酸水溶液为流动相,流速0.80mL·min-1;质谱采用电喷雾电离源正源(ESI+),定量分析采用选择离子监测(SIR),质荷比为准分子离子峰;血浆样品用乙腈沉淀后,将乙腈挥干,残渣用氯仿-异丙醇提取杂质,磷酸液提取药物,外标法定量.结果米格列醇浓度在32~3 200 ng·mL-1内线性关系良好,相关系数为0.999 7,最低检测浓度5ng·mL-1(S/N=3).批内、批间RSD符合方法学要求,单次服用100mg米格列醇片后药动学参数AUC0~12,AUC0~∞,cmax,tmax,t1/2分别为(7 592.7±4 207.4)ng·h·mL-1,(7 957.6±4 425.7)ng·h·mL-1,(1 883.9±912.3)ng·mL-1,(2.4±0.6)h,(2.5±0.9)h.结论该方法稳定、灵敏度高、操作简单,适用于米格列醇血药浓度测定及药动学研究.
语种:
中文
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葛根总黄酮生物黏附性缓释片在家犬体内的药动学及生物利用度研究
作者:
向大雄;李焕德;罗杰英;袁红波;张杰;...
期刊:
中国药学杂志 ,2005年40(7):532-534 ISSN:1001-2494
通讯作者:
Xiang, D.-X.(xiangdaxiong@netease.com)
作者机构:
[向大雄; 李焕德; 龚莉; 袁红波; 孙银香] Second Xiangya Hospital, Central Southern University, Changsha, 410011, China;[张杰] Pharmacy Department of Third Xangya Hospital, Central Southern University, Changsha 410013, China;[罗杰英] Hunan College of the Traditional Chinese Medicine, Changsha 410007, China
通讯机构:
[Xiang, D.-X.] S;Second Xiangya Hospital, Central Southern University, China
关键词:
葛根总黄酮;生物黏附;缓释片;药动学;生物利用度
摘要:
目的 研究葛根总黄酮(TPF)生物黏附性缓释片和普通片在家犬体内的药动学与生物利用度,揭示葛根总黄酮生物黏附性缓释片在家犬体内的药动学变化规律,为临床合理运用该药提供实验依据?方法健康家犬6只,以自身前后交叉对照方式,单剂量分别给予葛根总黄酮生物黏附性缓释片和普通片600mg。葛根素血药浓度经时数据以3P87药动学程序处理,以对两种片剂在家犬体内的动态过程进行拟合,以非室模型统计矩方法,计算药时曲线下面积(AUC0→2),并由血药浓度数据读取Cmax,tmax,经统计分析比较普通片与缓释片的生物利用度。结果单剂量给予家犬TPF片及生物黏附片后,葛根素血药浓度变化符合一级吸收一室模型,Ke分别为(0.461±0.115)h^-1,(0.238±0.042)h^-1;Ka分别为(0.844±0.404)h^-1,(0.446±0.100)h^-1;t1/2ka分别为(0.954±0.352)h,(1.609±0.292)h,t1/2ke分别为(1.570±0.333)h,(2.995±0.617)h。普通片的AUC0-12,Cmax,tmax分别为(4430±998)ng·h·mL^-1,(1241±247)ng·mL^-1和(2.2±0.6)h。缓释片的AUC0→12,cmax和tmax分别为(6459±738)ng·h·mL^-1,(1040±134)ng·mL^-1和(4.0±0.7)h。以普通片为参比制剂,缓释片的生物利用度为(151.4±37.6)%。结论葛根总黄酮缓释片在家犬体内表现出较好的缓释特性,主要药动学参数发生了变化,生物黏附片的生物利用度也明显高与普通片。
语种:
中文
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大孔吸附树脂分离纯化葛根总黄酮的研究
作者:
向大雄;李焕德;朱叶超;罗杰英
期刊:
中国药学杂志 ,2003年38(1):35-37 ISSN:1001-2494
通讯作者:
Xiang, D.-X.(xiangdaxiong@netease.com)
作者机构:
[李焕德; 向大雄] Second Xiangya Hospital, Central South University, Changsha 410011, China;[罗杰英] Hunan Coll. Traditional Chinese Med., Changsha 410007, China;[朱叶超] Daya Pharmaceutical Limited Company, Huizhou 516001, China
通讯机构:
[Xiang, D.-X.] S;Second Xiangya Hospital, Central South University, China
关键词:
葛根总黄酮;大孔吸附树脂;分离;纯化
摘要:
目的研究大孔吸附树脂分离纯化葛根总黄酮的工艺,为葛根总黄酮的工业化生产提供实验依据.方法采用D101型、D201型、AB-8型3种大孔吸附树脂对葛根总黄酮进行吸附纯化,以总黄酮收率、纯度为考察指标综合评价.结果D101型、D201型、AB-8型大孔吸附树脂吸附3种方法中静态饱和吸附量以干树脂计分别为113.5,133.1和133.9 mg@g-1;静态脱吸附率分别为75.6%,66.7%,94.2%;以70%的乙醇为洗脱剂洗脱率最高为92.4%;AB-8型吸附树脂饱和吸附-洗脱量为82.0 mg@g-1;稳定性实验中第11个周期总黄酮收率为91.3%,纯度为86.0%.结论3种纯化方法中,以AB-8型树脂综合性能最好,适合于葛根总黄酮的分离纯化.
语种:
中文
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Analysis of Polygonatum odoratum and its substitutes by RAPD
作者:
刘塔斯;李钟;刘春林
期刊:
中国药学杂志 ,2002年37(10):734-736 ISSN:1001-2494
通讯作者:
Liu, T.-S.(LiuTasi@163.net)
作者机构:
[刘塔斯; 李钟] 湖南中医学院;[刘春林] 湖南农业大学
通讯机构:
[Liu, T.-S.] H;Human Coll. Traditional Chinese Med., Changsha 410007, China
关键词:
玉竹;黄精;遗传多样性;中药鉴定
摘要:
目的鉴别中药玉竹Polygonatumodoratum及其混淆品,探索药材鉴别的新方法,为玉竹品质评价提供分子依据.方法用随机扩增多态性DNA(RAPD)技术进行基因组DNA多态性分析,并构建聚类树型图.结果玉竹、黄精遗传多态性丰富,道地药材玉竹和其他产地的玉竹已产生遗传分化.结论 RAPD技术可应用于药材玉竹的鉴定,结合药效成分分析,可以成为玉竹品质评价的有力工具.
语种:
中文
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新滩滑坡运动全过程的非连续变形分析与仿真模拟
作者:
殷坤龙;姜清辉;汪洋
期刊:
岩石力学与工程学报 ,2002年21(7):959-962 ISSN:1000-6915
通讯作者:
Yin, K.
作者机构:
国地质大学工程学院;武汉大学水电学院;国地质大学工程学院 武汉430074;武汉430072;[殷坤龙; 汪洋] 中国地质大学(武汉)
通讯机构:
Eng. Faculty, China Univ. of Geosci., China
关键词:
非连续变形分析;新滩滑坡;数值模拟
摘要:
简要介绍了非连续变形分析的基本原理,分析了新滩滑坡的工程地质特征和发展变化特征,并用非连续变形分析的方法对该滑坡运动的全过程进行了数值模拟,明确了其时空变化规律.非连续变形分析模拟结果表明,中部的姜家坡先发生滑动,继而对上部古滑体产生牵引作用,对下部古滑体产生推挤作用,导致新老崩积堆积区、毛家院和柳林地区的失稳,造成整体滑动.
语种:
中文
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低温固结K球团的还原
作者:
王颜平;孟香芝;唐敏;唐贤容
期刊:
矿冶工程 ,2001年21(1):46-48 ISSN:0253-6099
作者机构:
[王颜平; 唐敏] 湖南中医学院计算机中心;[孟香芝] 湖南制药厂;[唐贤容] 中南大学
关键词:
K球团;直接还原;计算机
摘要:
根据攀枝花钒钛磁铁精矿、冀东磁铁精矿和锌精矿等K球团(包括团矿)还原试验结果,探讨了K球团的还原机理;研究了影响还原反应速度的因素;提出了磁铁精矿K球团金属还原率(Ym)与还原时间(X1)、还原温度(X2)、C/Fe(X3)的二次旋转回归方程,在实际生产过程中可采用计算机对K球团的还原状况进行预测和控制。
语种:
中文
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关于分断常变元时滞微分方程解的振动性
作者:
郑洁钢
期刊:
湖南师范大学自然科学学报 ,2001年24(4):14-18 ISSN:2096-5281
通讯作者:
Zheng, J.-G.
作者机构:
[郑洁钢] Department of Basic Sciences, Hunan Coll. of Traditions Chinese, Changsha 410081, China
关键词:
振动性;微分方程;差分方程
摘要:
考虑分段常变元时滞微分方程x′(t)+a(t)x(t)+b(t)x([t-l])=0的振动性,其中a(t)和b(t)是在[-k,∞)上的连续函数,b(t)≥0,k是正整数[·]表示最大整函数,得到了一些新的振动条件.
语种:
中文
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