作者机构:
[Sheng, Wen] Hunan Univ Chinese Med, Coll Tradit Chinese Med, Changsha 410000, Peoples R China.;[Lu, Baowei; Sheng, Wen; Liu, Lumei; Ding, Jin; He, Qinghu] Hunan Univ Chinese Med, Androl Lab, Changsha 410000, Peoples R China.;[Xu, Wenjing] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Dermatol, Changsha 410000, Peoples R China.;[Ding, Jin] Guangzhou Univ Tradit Chinese Med, Affiliated Baoan Hosp Tradit Chinese Med, Clin Med Coll 7, Dept Androl, Shenzhen 518000, Peoples R China.;[Lu, Baowei; Liu, Lumei] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha 410000, Peoples R China.
通讯机构:
[Zhou, Q ; He, QH ] H;Hunan Univ Chinese Med, Androl Lab, Changsha 410000, Peoples R China.;Hunan Univ Med, Coll Tradit Chinese Med, Changsha 410000, Peoples R China.;Hunan Univ Chinese Med, Hosp 1, Dept Androl, Changsha 410000, Peoples R China.
关键词:
Colla Carapacis et Plastri;Fecal microbiota transplantation;Male infertility;Gut microbiota;Spermatogenesis ability
摘要:
[ABSTRACT] Male infertility is a significant cause of psychosocial and marital distress in approximately 50% of couples who are unable to conceive, with male factors being the underlying cause. Guijiajiao (Colla Carapacis et Plastri, CCP) is a Traditional Chinese Medicine commonly used to treat male infertility. The present study aimed to investigate the potential mechanisms underlying the preventive effects of CCP on male infertility. An infertile male rat model was established using cyclophosphamide (CTX), and CCP was administered for both treatment and prevention. Fecal microbiota transplantation (FMT) was also performed to explore the role of gut microbiota in the CCP-mediated prevention of male infertility in rats. Sperm motility and concentration were determined using a semiautomatic sperm classification analyzer. Subsequently, histopathological analysis using HE staining was performed to examine the changes in the small intestine and testis. Moreover, the serum levels of lipopolysaccharide (LPS) and testosterone were measured by ELISA. In addition, immunohistochemistry was conducted to detect CD3 expression in the small intestine, while RT-qPCR was employed to assess the expressions of interleukin-1 beta (IL-10), cluster of differentiation 3 (CD3), Monocyte chemoattractant protein-1 (MCP-1), and C-X-C motif chemokine ligand 10 (CXCL-10) in the small intestine and epididymis. Finally, gut microbiota was analyzed by 16S rRNA sequencing. CCP improved sperm motility, number, and concentration in CTX-induced infertile male rats. CCP increased the serum testosterone level, inhibited the immune cell infiltration of the intestinal lamina propria, and promoted the aggregation of CD3+ T cells in CTX-induced male infertility rats. CCP also inhibited the expressions of MCP-1, CXCL-10, and IL-10 in the epididymis of male infertility rats. At the genus level, CTX led to a reduction in the abundance of Lactobacillus, Clostridia_UCG.014, and Romboutsia in the intestinal tract of rats. In contrast, CCP decreased the abundance of Ruminococcus and increased the abundance of Romboutsia in infertile male rats. Additionally, FMT experiments proved that the gut microbiota of CCP-treated rats facilitated testicular tissue recovery and spermatogenesis while also reducing the serum LPS level in infertile male rats. CCP improves the spermatogenic ability of infertile male rats by restoring gut microbiota diversity and inhibiting epididymal inflammation.
摘要:
Atherosclerosis (AS) is the major form of cardiovascular disease and the leading cause of morbidity and mortality in countries around the world. Atherosclerosis combines the interactions of systemic risk factors, haemodynamic factors, and biological factors, in which biomechanical and biochemical cues strongly regulate the process of atherosclerosis. The development of atherosclerosis is directly related to hemodynamic disorders and is the most important parameter in the biomechanics of atherosclerosis. The complex blood flow in arteries forms rich WSS vectorial features, including the newly proposed WSS topological skeleton to identify and classify the WSS fixed points and manifolds in complex vascular geometries. The onset of plaque usually occurs in the low WSS area, and the plaque development alters the local WSS topography. low WSS promotes atherosclerosis, while high WSS prevents atherosclerosis. Upon further progression of plaques, high WSS is associated with the formation of vulnerable plaque phenotype. Different types of shear stress can lead to focal differences in plaque composition and to spatial variations in the susceptibility to plaque rupture, atherosclerosis progression and thrombus formation. WSS can potentially gain insight into the initial lesions of AS and the vulnerable phenotype that gradually develops over time. The characteristics of WSS are studied through computational fluid dynamics (CFD) modeling. With the continuous improvement of computer performance-cost ratio, WSS as one of the effective parameters for early diagnosis of atherosclerosis has become a reality and will be worth actively promoting in clinical practice. The research on the pathogenesis of atherosclerosis based on WSS is gradually an academic consensus. This article will comprehensively review the systemic risk factors, hemodynamics and biological factors involved in the formation of atherosclerosis, and combine the application of CFD in hemodynamics, focusing on the mechanism of WSS and the complex interactions between WSS and plaque biological factors. It is expected to lay a foundation for revealing the pathophysiological mechanisms related to abnormal WSS in the progression and transformation of human atherosclerotic plaques.
期刊:
Frontiers in Microbiology,2023年14:1288430 ISSN:1664-302X
通讯作者:
Jiang, Ping;Tan, ZJ;Jiang, P
作者机构:
[Tan, Zhoujin; Liu, Jing; Wu, Yi; Jiang, Ping; Deng, Na] Hunan Univ Chinese Med, Coll Tradit Chinese Med, Changsha, Peoples R China.;[Jiang, Ping] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha, Peoples R China.
通讯机构:
[Jiang, P; Tan, ZJ ; Jiang, P ] H;Hunan Univ Chinese Med, Coll Tradit Chinese Med, Changsha, Peoples R China.;Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha, Peoples R China.
关键词:
Chinese medicine;cold-dampness trapped spleen syndrome;diarrhea;digestive enzyme activities;intestinal microbiota
摘要:
INTRODUCTION: Cold and humid environments alter the intestinal microbiota, and the role of the intestinal microbiota in the development of diarrhea associated with cold-dampness trapped spleen syndrome in Chinese medicine is unclear. METHODS: The 30 mice were randomly divided into normal and model groups, with the model group being exposed to cold and humid environmental stresses for 7 days. Then, mouse intestinal contents were collected and analyzed their intestinal microbiota and digestive enzymes. RESULTS: Our findings revealed significant increases in sucrase and lactase activities, as well as microbial activity, in the model group (p < 0.05). β-diversity analysis highlighted distinct intestinal microbiota compositions between the two groups. Specifically, the experimental group showed a unique dominance of the genera and strains Clostridium sensu stricto 1 and Clostridium sp. ND2. LEfSe analysis identified Helicobacter, Roseburia, and Eubacterium plexicaudatum ASF492 as differentially abundant species in them model group. Network analysis demonstrated that rare bacterial species mostly governed the microbial interactions, exhibiting increased mutual promotion. On the other hand, abundant species like Lactobacillus johnsonii and Lactobacillus reuteri showed mutual inhibitory relationships. DISCUSSION: In summary, exposure to cold and humid conditions led to increased intestinal enzyme activities and a shift in microbial composition, favoring the growth of rare bacterial species. These changes suggest that rare bacteria in the intestinal microbiota play a critical role in the pathology of diarrhea associated with cold-dampness trapped spleen syndrome, revealing unique survival strategies among bacterial populations under stressful conditions.
摘要:
Immune-related adverse events (irAEs), including skin injury, liver and kidney injury, colitis, as well as cardiovascular adverse events, are a series of complications arising during the treatment of immune checkpoint inhibitors (ICIs). Cardiovascular events are the most urgent and the most critical, as they can end life in a short period of time. With the widespread use of ICIs, the number of immune-related cardiovascular adverse events (irACEs) induced by ICIs has increased. More attention has been paid to irACEs, especially regarding cardiotoxicity, the pathogenic mechanism, diagnosis and treatment. This review aims to assess the risk factors for irACEs, to raise awareness and help with the risk assessment of irACEs at an early stage.
期刊:
Frontiers in Medicine,2023年10:1259182 ISSN:2296-858X
通讯作者:
Hu, ZX
作者机构:
[Hu, Zhixi; Li, Lin; Hu, ZX; Hu, Siyuan] Hunan Univ Chinese Med, Domest class Discipline Construct Project Chinese, Changsha, Hunan, Peoples R China.;[Hu, Zhixi; Li, Lin; Hu, ZX] Hunan Univ Chinese Med, Prov Key Lab TCM Diagnost, Changsha, Hunan, Peoples R China.;[Li, Lin] Hunan Engn Technol Res Ctr Med & Funct Food, Changsha, Hunan, Peoples R China.;[Zhong, Senjie; Ye, Jiahao] Guangzhou Univ Chinese Med, Affiliated Hosp 1, Guangzhou, Guangdong, Peoples R China.;[Ye, Jiahao] Hunan Univ Chinese Med, Postgrad Sch, Changsha, Hunan, Peoples R China.
通讯机构:
[Hu, ZX ] H;Hunan Univ Chinese Med, Domest class Discipline Construct Project Chinese, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Prov Key Lab TCM Diagnost, Changsha, Hunan, Peoples R China.
关键词:
Heart Failure;Danhong injection;Metabolomics;transverse aortic constriction;Chinese medicine (CM)
摘要:
BackgroundHeart failure (HF) is characterized by reduced ventricular filling or ejection function due to organic or non-organic cardiovascular diseases. Danhong injection (DHI) is a medicinal material used clinically to treat HF for many years in China. Although prior research has shown that Danhong injection can improve cardiac function and structure, the biological mechanism has yet to be determined.MethodsSerum metabolic analysis was conducted via ultra-high-performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UHPLC-QE/MS) to explore underlying protective mechanisms of DHI in the transverse aortic constriction (TAC)-induced heart failure. Multivariate statistical techniques were used in the research, such as unsupervised principal component analysis (PCA) and orthogonal projection to latent structures discriminant analysis (OPLS-DA). MetaboAnalyst and Kyoto Encyclopedia of Genes and Genomes (KEGG) were employed to pinpoint pertinent metabolic pathways.ResultsAfter DHI treatment, cardiac morphology and function as well as the metabolism in model rats were improved. We identified 17 differential metabolites and six metabolic pathways. Two biomarkers, PC(18:3(6Z,9Z,12Z)/24:0) and L-Phenylalanine, were identified for the first time as strong indicators for the significant effect of DHI.ConclusionThis study revealed that DHI could regulate potential biomarkers and correlated metabolic pathway, which highlighted therapeutic potential of DHI in managing HF.
作者机构:
[Zhang, Xiang-Zhuo; Huang, Shu-Chun; Li, Jing; Yang, Yang; Kuang, Hui-Fang] Hunan Univ Chinese Med, Coll Tradit Chinese Med, Changsha 410208, Hunan, Peoples R China.;[Su, Chang] Hunan Univ Chinese Med, Coll Xiangxing, Changsha 410208, Hunan, Peoples R China.;[Zhang, Qiu-Yan] Hunan Univ Chinese Med, Sch Infirm, Changsha 410208, Hunan, Peoples R China.
通讯机构:
[Kuang, Hui-Fang; Su, Chang; Zhang, Xiang-Zhuo; Huang, Shu-Chun; Yang, Yang; Li, Jing] C;[Zhang, Qiu-Yan] S;College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China. Electronic address:;College of Xiangxing, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China. Electronic address:;School Infirmary, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China. Electronic address:
摘要:
Coronary heart disease (CHD) has become the leading cause of mortality, morbidity, and disability worldwide. Though the therapeutic effect of Xuefu Zhuyu Decoction (XFZY) on CHD has been demonstrated in China, the active ingredients and molecular mechanisms of XFZY have not been elucidated. The purpose of the current study is to explore the molecular mechanisms of XFZY in the treatment of CHD via network pharmacology, metabolomics, and experimental validation. First, we established a CHD rat model by permanently ligating the left anterior descending coronary artery (LAD), and evaluated the therapeutic effect of XFZY by hemorheology and histopathology. Second, network pharmacology was employed to screen the active ingredients and potential targets of XFZY for the treatment of CHD. Metabolomic was applied to identify the molecules present in the serum after XFZY treatment. Third, the results of network pharmacology and metabolomics were further analyzed by Cytoscape to elucidate the core ingredients and pathways. Finally, the obtained key pathways were verified by transmission electron microscopy and immunofluorescence assay. The results showed that XFZY was effective in the treatment of CHD in the rat model, and the highest dose exerted the best effect. Network pharmacology analysis revealed 215 active ingredients and 129 key targets associated with XFZY treatment of CHD. These targets were enriched in pathways of cancer, lipid and atherosclerosis, fluid shear stress and atherosclerosis, proteoglycans in cancer, chemical carcinogenesis - receptor activation, HIF-1 signaling, et al. Serum metabolomic identified 1081 metabolites involved in the therapeutic effect of XFZY on CHD. These metabolites were enriched in taurine and hypotaurine metabolism, histidine metabolism, retrograde endocannabinoid signaling pathways, et al. Cytoscape analysis combining the data from serum metabolomic and network pharmacology revealed that energy metabolism as the core pathway for XFZY treatment of CHD. Electron microscope observation identified changes in the level of autophagy in the mitochondrial structure of cardiomyocytes. Immunofluorescence assay showed that the expression levels of autophagy-related proteins LC3-B and P62/SQSTM1 were consistent with the levels of autophagy observed in mitochondria. In conclusion, our findings suggest that the possible mechanisms of XFZY in the treatment of CHD are reducing the level of autophagy, improving energy metabolism, and maintaining mitochondrial homeostasis in cardiomyocytes. Our study also shows that the combined strategies of network pharmacology, metabolomics, and experimental validation may provide a powerful approach for TCM pharmacology study.
作者机构:
[Tian, He-Yan] Shenzhen Polytech Univ, Sch Med Technol & Nursing, Shenzhen 518000, Peoples R China.;[Yang, Tong; Huang, Bo-Yang; Nie, Hui-Fang; Cheng, Shao-Wu; Mei, Zhi-Gang; Chen, Xiang-Yu; Ge, Jin-Wen; Zhou, Yue] Hunan Univ Chinese Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha 410208, Peoples R China.;[Ge, Jin-Wen] Hunan Acad Tradit Chinese Med, Inst Chinese Med, Changsha 410208, Peoples R China.
通讯机构:
[Mei, ZG; Ge, JW ] H;Hunan Univ Chinese Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha 410208, Peoples R China.;Hunan Acad Tradit Chinese Med, Inst Chinese Med, Changsha 410208, Peoples R China.
关键词:
cerebral ischemia;central nervous system diseases;mitochondrial dysfunction;ferroptosis;therapeutics
摘要:
Cerebral ischemia, a leading cause of disability and mortality worldwide, triggers a cascade of molecular and cellular pathologies linked to several central nervous system (CNS) disorders. These disorders primarily encompass ischemic stroke, Alzheimer's disease (AD), Parkinson's disease (PD), epilepsy, and other CNS conditions. Despite substantial progress in understanding and treating the underlying pathological processes in various neurological diseases, there is still a notable absence of effective therapeutic approaches aimed specifically at mitigating the damage caused by these illnesses. Remarkably, ischemia causes severe damage to cells in ischemia-associated CNS diseases. Cerebral ischemia initiates oxygen and glucose deprivation, which subsequently promotes mitochondrial dysfunction, including mitochondrial permeability transition pore (MPTP) opening, mitophagy dysfunction, and excessive mitochondrial fission, triggering various forms of cell death such as autophagy, apoptosis, as well as ferroptosis. Ferroptosis, a novel type of regulated cell death (RCD), is characterized by iron-dependent accumulation of lethal reactive oxygen species (ROS) and lipid peroxidation. Mitochondrial dysfunction and ferroptosis both play critical roles in the pathogenic progression of ischemia-associated CNS diseases. In recent years, growing evidence has indicated that mitochondrial dysfunction interplays with ferroptosis to aggravate cerebral ischemia injury. However, the potential connections between mitochondrial dysfunction and ferroptosis in cerebral ischemia have not yet been clarified. Thus, we analyzed the underlying mechanism between mitochondrial dysfunction and ferroptosis in ischemia-associated CNS diseases. We also discovered that GSH depletion and GPX4 inactivation cause lipoxygenase activation and calcium influx following cerebral ischemia injury, resulting in MPTP opening and mitochondrial dysfunction. Additionally, dysfunction in mitochondrial electron transport and an imbalanced fusion-to-fission ratio can lead to the accumulation of ROS and iron overload, which further contribute to the occurrence of ferroptosis. This creates a vicious cycle that continuously worsens cerebral ischemia injury. In this study, our focus is on exploring the interplay between mitochondrial dysfunction and ferroptosis, which may offer new insights into potential therapeutic approaches for the treatment of ischemia-associated CNS diseases.
摘要:
Plants from the Asteraceae family are widely used as ethno medicines to treatment parasitic, malaria, hematemesis, pruritus, pyretic, anthelmintic, wound healing. The aim of this review is to provide an overview of Asteraceae plants antimicrobial activity. The most relevant results from the published studies are summarized and discussed. The species in genus of Artemisia, Echinacea, Centaurea, Baccharis, and Calendula showed antimicrobial activity. Most of these species are usually used as ethno medicines to treat infection, inflammation, and parasitics. The effective part or component for antimicrobial was essential oil and crude extract, and essential oil attracted more attention. It was also reported that nanoparticles coated with crude extract were effective against multidrug resistant bacteria. For multidrug resistant bacteria study, the species in Armtemisia were the most investigated, and Staphylococcus aureus and Escherichia coli were the most studied multidrug resistant strains. The antimicrobial activity was evaluated mainly based on the results of minimum inhibitory concentration (MIC). Few reports have been reported on minimum bactericide concentration (MBC) and its antibacterial mechanisms. According to the reported study results, some plants in Asteraceae have the potential to be developed as bacteriostatic agents and against multidrug resistant bacteria. However, most studies are still in vitro, further clinical and applied studies are needed.
作者机构:
[Huang, Jiawang; Ma, Xinyue; Li, Ling; Liao, Zexuan; Liu, Zhuolin] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha 410208, Peoples R China.;[Feng, Zhiying; Wang, Kangyu] Hunan Univ Chinese Med, Coll Tradit Chinese Med, Changsha 410208, Peoples R China.;[Ning, Yi; Lu, Fangguo] Hunan Univ Chinese Med, Med Sch, Changsha 410208, Peoples R China.;[Li, Ling; Liu, Zhuolin] Hunan Univ Chinese Med, Hunan Prov Key Lab Integrated Tradit Chinese & Wes, Changsha 410208, Peoples R China.
通讯机构:
[Li, L ] H;Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha 410208, Peoples R China.;Hunan Univ Chinese Med, Hunan Prov Key Lab Integrated Tradit Chinese & Wes, Changsha 410208, Peoples R China.
摘要:
Influenza is an acute viral respiratory infection that has caused high morbidity and mortality worldwide. Influenza A virus (IAV) has been found to activate multiple programmed cell death pathways, including ferroptosis. Ferroptosis is a novel form of programmed cell death in which the accumulation of intracellular iron promotes lipid peroxidation, leading to cell death. However, little is known about how influenza viruses induce ferroptosis in the host cells. In this study, based on network pharmacology, we predicted the mechanism of action of Maxing Shigan decoction (MXSGD) in IAV-induced ferroptosis, and found that this process was related to biological processes, cellular components, molecular function and multiple signaling pathways, where the hypoxia inducible factor-1(HIF-1) signaling pathway plays a significant role. Subsequently, we constructed the mouse lung epithelial (MLE-12) cell model by IAV-infected in vitro cell experiments, and revealed that IAV infection induced cellular ferroptosis that was characterized by mitochondrial damage, increased reactive oxygen species (ROS) release, increased total iron and iron ion contents, decreased expression of ferroptosis marker gene recombinant glutathione peroxidase 4 (GPX4), increased expression of acyl-CoA synthetase long chain family member 4 (ACSL4), and enhanced activation of hypoxia inducible factor-1 alpha (HIF-1 alpha), induced nitric oxide synthase (iNOS) and vascular endothelial growth factor (VEGF) in the HIF-1 signaling pathway. Treatment with MXSGD effectively reduced intracellular viral load, while reducing ROS, total iron and ferrous ion contents, repairing mitochondrial results and inhibiting the expression of cellular ferroptosis and the HIF-1 signaling pathway. Finally, based on animal experiments, it was found that MXSGD effectively alleviated pulmonary congestion, edema and inflammation in IAV-infected mice, and inhibited the expression of ferroptosis-related protein and the HIF-1 signaling pathway in lung tissues.
期刊:
Skin Research and Technology,2023年29(9) ISSN:0909-752X
通讯作者:
Li, X
作者机构:
[Li, Xiaosha; Wang, Haizhen] Hunan Univ Chinese Med, Affiliated Hosp 2, Dept Dermatol, Changsha, Peoples R China.;[Tang, Shiyang] Hunan Univ Chinese Med, Sch Chinese Med, Changsha, Peoples R China.;[Li, Xin] Hunan Univ Chinese Med, Hunan Prov Key Lab Diagnost Chinese Med, 300 Xueshi Rd, Hanpu Sci & Educ Pk, Changsha 410208, Hunan, Peoples R China.
通讯机构:
[Li, X ] H;Hunan Univ Chinese Med, Hunan Prov Key Lab Diagnost Chinese Med, 300 Xueshi Rd, Hanpu Sci & Educ Pk, Changsha 410208, Hunan, Peoples R China.
关键词:
NLRP3;pyroptosis;ROS;tanshinoneIIA;vitiligo
摘要:
Abstract Background Pyroptosis has been implicated in the development of human diseases, including vitiligo. TanshinoneIIA has been confirmed to play anti‐vitiligo role. However, whether tanshinoneIIA inhibits vitiligo progression via regulating cell pyroptosis remains unclear. Methods Hydrogen peroxide (H2O2)‐induced melanocytes were used to mimic vitiligo cell model in vitro. Cell viability was assessed by cell counting kit 8 assay, and reactive oxygen species (ROS) production was detected by DCFH‐DA staining. Nod‐like receptor protein 3 (NLRP3) expression was detected by quantitative real‐time PCR, western blot and immunofluorescence staining. Cell pyroptosis was measured using flow cytometry, and the contents of interleukin‐1β and interleukin‐18 were determined by ELISA. Besides, the protein levels of apoptosis‐associated speck‐like protein containing CARD (ASC) and cleaved‐Caspase‐1 were examined by western blot analysis. Results H2O2 could induce ROS production, NLRP3 expression and pyroptosis in melanocytes. TanshinoneIIA inhibited ROS production, pyroptosis, and the expression of NLRP3, ASC and cleaved‐caspase‐1 in H2O2‐induced melanocytes. Compared with the function of ROS inhibitor (NAC), tanshinoneIIA acted as a ROS scavenger to relieve melanocyte pyroptosis. In addition, NLRP3 inhibitor (MCC950) also could aggravate the inhibition effect of tanshinoneIIA on melanocyte pyroptosis. Conclusion TanshinoneIIA suppressed melanocyte pyroptosis probably through modulating the ROS/NLRP3 signaling axis, which provides the evidence for therapeutic effect in vitiligo.
摘要:
BACKGROUND: Spinal cord injury (SCI) refers to the interruption of the tracts inside the spinal cord caused by various factors. The repair of damaged axons has always been a difficult point in clinical treatment and neuroscience research. The treatment of SCI with Buyang huanwu decoction (BYHWD), a well-known recipe for invigorating Qi (a vital force forming part of any living entity in traditional Chinese culture) and promoting blood circulation, shows a good effect. METHODS: The rubrospinal tract (RST) transection model in rats was established in this study and rats were administrated with low (BL), medium (BM), or high (BH) doses of BYHWD. RESULTS: Compared with the SCI group, BL, BM moderately, and BH significantly improved the motor function of forelimbs and increased the number of red nucleus neurons in SCI rats. As for the possible molecular mechanism, BL, BM moderately, and BH significantly increased mTOR whereas decreased Beclin-1 and LC3 in the red nucleus. CONCLUSION: In conclusion, low, medium, and high doses of BYHWD could promote neural recovery in SCI rats through improving motor function and neuron survival in the red nucleus. The neuroprotective effects of BYHWD might be associated with affecting the mTOR signaling pathway and autophagy.
作者机构:
[Yu Yi-Pin; Sheng Dan; Zhong Li-Qin; Tan Duo-Ting] Hunan Univ Chinese Med, Grad Sch, Changsha 410208, Peoples R China.;[Liang, H; Hu Zhi-Xi; Liang Hao; Yang Liu] Hunan Univ Chinese Med, Inst TCM Diagnost, Changsha 410208, Peoples R China.;[Huang Ru-Jia] Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Changsha 410208, Peoples R China.
通讯机构:
[Liang, H ] H;Hunan Univ Chinese Med, Inst TCM Diagnost, Changsha 410208, Peoples R China.
作者:
Huang, Yalan;Zhang, Yanling;Wu, Yongjun;Xiang, Qin;Yu, Rong
期刊:
Drug Design, Development and Therapy,2023年17:237-260 ISSN:1177-8881
通讯作者:
Yu, R.;Xiang, Q.
作者机构:
[Huang, Yalan; Yu, Rong] Hunan Univ Tradit Chinese Med, Grad Sch, Changsha 410208, Peoples R China.;[Huang, Yalan] Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, Changsha 410021, Peoples R China.;[Zhang, Yanling] Hunan Univ Tradit Chinese Med, Coll Tradit Chinese Med, Changsha 410208, Peoples R China.;[Zhang, Yanling] Ningxia Med Univ, Gen Hosp, Ningxia 750003, Peoples R China.;[Wu, Yongjun] Hunan Univ Tradit Chinese Med, Coll Pharm, Changsha 410208, Peoples R China.
通讯机构:
[Qin Xiang] S;[Rong Yu] G;Science and Technology Department, Hunan University of Traditional Chinese Medicine, Changsha, 410208, People’s Republic of China<&wdkj&>Graduate School, Hunan University of Traditional Chinese Medicine, Changsha, 410208, People’s Republic of China
关键词:
apoptosis;diabetic cardiomyopathy;network pharmacology;pharmacochemistry;PI3K/Akt pathway;Zuogui Jiangtang Shuxin formula
摘要:
Prolonged exposure of the peritoneum to high glucose dialysate leads to the development of peritoneal fibrosis (PF), and apoptosis of peritoneal mesothelial cells (PMCs) is a major cause of PF. The aim of this study is to investigate whether Astragaloside IV could protect PMCs from apoptosis and alleviate PF. PMCs and rats PF models were induced by high glucose peritoneal fluid. We examined the pathology of rat peritoneal tissue by HE staining, the thickness of rat peritoneal tissue by Masson's staining, the number of mitochondria and oxidative stress levels in peritoneal tissue by JC-1 and DHE fluorescence staining, and mitochondria-related proteins and apoptosis-related proteins such as PGC-1α, NRF1, TFAM, Caspase3, Bcl2 smad2 were measured. We used hoechst staining and flow cytometry to assess the apoptotic rate of PMCs in the PF model, and further validated the observed changes in the expressions of PGC-1α, NRF1, TFAM, Caspase3, Bcl2 smad2 in PMCs. We further incubated PMCs with MG-132 (proteasome inhibitor) and Cyclohexylamine (protein synthesis inhibitor). The results demonstrated that Astragaloside IV increased the expression of PGC-1α by reducing the ubiquitination of PGC-1α. It was further found that the protective effects of Astragaloside IV on PMCs were blocked when PGC-1α was inhibited. In conclusion, Astragaloside IV effectively alleviated PF both in vitro and in vivo, possibly by promoting PGC-1α to enhance mitochondrial synthesis to reduce apoptotic effects.
摘要:
Osteoarthritis (OA) is a chronic joint disease characterized by progressive cartilage degeneration, which imposes a heavy physical and financial burden on the middle-aged and elderly population. As the pathogenesis of OA has not been fully elucidated, it is of great importance to develop targeted therapeutic or preventive medications. Traditional therapeutic drugs, such as non-steroidal anti-inflammatory drugs, steroids and opioids, have significant side effects, making the exploration for safe and effective alternative therapeutic drugs urgent. In recent years, many studies have reported the role of plant-derived polysaccharides in anti-inflammation, anti-oxidation, regulation of chondrocyte metabolism and proliferation, and cartilage protection, and have demonstrated their great potential in the treatment of OA. Therefore, by focusing on studies related to the intervention of plant-derived polysaccharides in OA, including in vivo and in vitro experiments, this review aimed to classify and summarize the existing research findings according to different mechanisms of action. In addition, reports on plant-derived polysaccharides as nanoparticles were also explored. Then, candidate monomers and theoretical bases were provided for the further development and application of novel drugs in the treatment of OA.
期刊:
European Journal of Integrative Medicine,2023年58:102222 ISSN:1876-3820
通讯作者:
Zhixi Hu
作者机构:
[Yi, Shuai; Jiang, Yang] Hunan Acad Tradit Chinese Med, Affiliated Hosp, Dept gastroenterol, Changsha 410006, Peoples R China.;[Yan, Xu] Hunan Acad Tradit Chinese Med, Affiliated Hosp, Dept cardiol, Changsha 410006, Peoples R China.;[Hu, Zhixi] Hunan Univ Chinese Med, Coll Tradit Chinese Med, Changsha 410208, Peoples R China.
通讯机构:
[Zhixi Hu] C;College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China
关键词:
Chronic heart failure;Traditional Chinese medicine;Biomarker;Heart -yang deficiency syndrome;Heart -yin deficiency syndrome;Case -control study
摘要:
Introduction: The recognition of serum biomarkers for differentiation between traditional Chinese medicine (TCM) syndromes would greatly increase the objectivity and treatment results. The objective of this study was to identify whether adiponectin (ADIPOQ), insulin-like growth factor binding protein 2 (IGFBP-2), and insulin-like growth factor binding protein 4 (IGFBP-4) are reliable biomarkers for differentiation between the heart-yang deficiency and heart-yin deficiency syndromes of TCM-based chronic heart failure.Methods: Serum samples of 25 heart-yang deficiency patients, 25 heart-yin deficiency patients, and 25 healthy participants were analyzed. The participants were also subjected to blood pressure measurements and laboratory testing of the variables creatinine, CRP, BNP, NT-proBNP, IGFBP-2, ADIPOQ, and IGFBP-4. ELISA assays were performed to confirm the expression patterns of IGFBP-2, ADIPOQ, and IGFBP-4. ANOVA was used to compare variables between groups and the Tukey's Test was applied to statistically significant results for post-hoc analysis. Logistic regression analysis was used to evaluate the relationship between IGFBP-4 and dichotomous variables, while linear regression analysis was used to evaluate continuous variables. A receiver operating characteristic (ROC) curve was plotted to evaluate the diagnostic ability of IGFBP-4.Results: The ELISA results showed that only IGFBP-4 was significantly up-regulated (p < 0.01) in heart-yang deficiency patients, while ADIPOQ and IGFBP-2 were significantly upregulated in both groups. The ROC curve suggested that the area under the curve value of IGFBP-4 was as high as 0.969.Conclusions: The results suggest IGFBP-4 may have diagnostic value as a potential biomarker for clinical dif-ferentiation between heart-yin deficiency and heart-yang deficiency patients.
摘要:
Luteolin has been reported to exhibit therapeutic effect on depressive-like behaviors in mice. Nevertheless, the therapeutic effect of luteolin on the depression-related dry eye disorder remains inconclusive. In this study, C57 mice were subjected to chronic unpredictable mild stress in a dry environment (relative humidity in the cage < 40%). The behavioral test and phenol red cotton thread test were employed to select the mice with both dry eye and depression-like behavior. The mechanism of luteolin on depression-related dry eye disorder was assessed by the Sirt1 selective inhibitor EX-527. Luteolin alleviated depressive-like behaviors induced by CUMS, increased tear secretion and restored corneal defects in mice. The secretions of pro-inflammatory factors IL-18, IL-6, IL-18 and TNF-alpha were decreased in hippocampi and corneal tissues by Luteolin treatment. Luteolin treatment up-regulated Sirt1 expression and down-regulated Ac-NF-KB, NLRP3, Ac-Caspase-1, GSDMD-N, Cleaved IL-18, and Cleaved IL-18 expressions. In addition, the selective inhibition of Sirt1 could weaken the therapeutic effect of luteolin on depression-related dry eye disorder. The beneficial effect of luteolin through Sirt1/NF-KB/NLRP3 signaling pathway might be a therapeutic strategy for the depression -related dry eye disorder.
期刊:
Annals of Translational Medicine,2022年10(18) ISSN:2305-5839
作者机构:
[Huang, Yalan; Yu, Rong] Hunan Univ Tradit Chinese Med, Coll Grad, Changsha, Peoples R China.;[Cai, Linkun] Guangdong Prov Hosp Tradit Chinese Med, Dept Gastroenterol, Guangzhou, Peoples R China.;[Liu, Xiu] Hunan Univ Tradit Chinese Med, Coll Tradit Chinese Med, Changsha, Peoples R China.;[Wu, Yongjun] Hunan Univ Tradit Chinese Med, Coll Pharm, Changsha, Peoples R China.;[Xiang, Qin] Hunan Univ Tradit Chinese Med, Sci & Technol Dept, Changsha, Peoples R China.
关键词:
Type 2 diabetes (T2D);RNA-seq;single-cell RNA (sc-RNA)-seq;biomarkers;transcription factors (TFs)
摘要:
Background: Type 2 diabetes (T2D) is a prevalent chronic disease with elusive. Combining transcriptome and single-cell sequencing data to explore biomarkers of T2D could provide new insights into the in-depth understanding of the molecular mechanisms and diagnosis of T2D. Methods: The GSE41762 dataset including RNA-seq data for healthy and T2D patients, was obtained from the Gene Expression Omnibus (GEO) database. The potential functions of the differentially expressed genes (DEGs) were revealed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Moreover, biomarkers were screened out by the Least Absolute Shrinkage and Selection Operator (LASSO) algorithm and receiver operating characteristic (ROC) analysis. Furthermore, single-cell RNA (sc-RNA)-seq data in the "E-MTAB-5061" dataset was downloaded from the ArrayExpress (European Bioinformatics Institute, EBI) database. Principal components analysis (PCA) and t-distributed stochastic neighbor embedding (tSNE) were used for dimensionality reduction analysis and cell clustering. The FindAllMarkers function was used annotate different cell clusters, and key cell clusters were screened by the expression levels of the biomarkers. Finally, the transcription factors (TFs) of the biomarkers were recognized. Results: A total of 111 DEGs were screened in the GSE41762 dataset, which were mainly related to hormone secretion, specialized postsynaptic membrane, pancreatic secretion, JAK-STAT signaling pathway, and Ras signaling pathway. In addition, SLC2A2, SERPINF1, RASGRP1, and CHL1 were screened out as biomarkers of T2D, which possessed potential diagnostic value as AUC value greater than 0.8. A total of 1,515 T2D group cells and 1,817 healthy cohort cells were screened as core cells in the "E-MTAB-5061" dataset. Following tSNE dimensionality reduction cluster analysis, the core cells were divided into 13 cell clusters. According to the marker genes, the 13 cell clusters were annotated into six types of cells. Notably, SERPINF1 was highly expressed in fibroblasts and might be regulated by NR2F2 (nuclear receptor subfamily2, group F, and member 2). Conclusions: This study identified four biomarkers (SLC2A2, SERPINF1, RASGRP1, and CHL1) for T2D, which provided new markers for the clinical diagnosis of T2D. Among them, SERPINF1 might be regulated by NR2F2, which provides valuable insight into the pathogensis of T2D.
摘要:
BACKGROUND: Osteoporosis (OP) has emerged as a major global public health issue due to its high prevalence, unknown pathogenesis, and lack of specific drugs for prevention and treatment. Studies have demonstrated that acupuncture in combination with Chinese herbal medicine (CHM) is effective in treating OP. However, there is a scarcity of experience and high-quality evidence. A network meta-analysis and systematic review were used to evaluate the efficacy and safety of acupuncture in combination with CHM for the treatment of OP. METHODS: Comprehensive search of Chinese databases such as China National Knowledge Infrastructure, VIP, Wanfang, China Biomedical Literature Database and English databases for example PubMed, Cochrane Library, EMbase, etc. The search period was extended from the creation of the database to November 2022. All randomized controlled trials on acupuncture in combination with CHM in dealing with OP were collected. After literature analysis and data extraction, the Cochrance system was used to evaluate the high quality of the included articles and Stata 14.0 was used for the network meta-analysis. RESULTS: The current systematic review and network meta-analysis will provide the effectiveness and safety of acupuncture in combination with CHM in dealing with OP. CONCLUSION: The research will provide reliable evidence for the clinical use of acupuncture in combination with CHM in dealing with OP.