摘要：
Background: Chronic heart failure (CHF) is a common and difficult-to-treat disease in clinical practice. The efficacy and safety of Zhenyuan capsule (ZYC) in the treatment of CHF were evaluated by meta-analysis and trial sequential analysis (TSA) of published relevant data. Methods: Searched 8 databases for clinical literature on ZYC in the treatment of CHF, up to December 2022. Then the meta-analysis and TSA were performed on the studies that met the inclusion criteria. Results: Meta-analysis showed that compared with conventional treatment, combined use of ZYC could significantly increase the clinical effective rate (risk ratio 1.20, 95% confidence interval [CI] 1.14 similar to 1.26, P < .00001) by 20%, left ventricular ejection fraction (MD 8.85, 95%CI 4.57 similar to 13.12, P < .0001) by 8.85%, and 6-minutes walking distance (MD 47.91, 95%CI 18.66 similar to 77.17, P = .001) by 47.91 m, and significantly reduce brain natriuretic peptide (MD -247.86, 95%CI -330.62 similar to-165.09, P < .00001) by 247.86 pg/mL. TSA showed that the benefits suggested by the original results were conclusive. In terms of safety, the total adverse events in the combined group of ZYC were comparable to those in the conventional group, and TSA demonstrated that this result needed more research and demonstration. Conclusion: ZYC can effectively improve the clinical efficacy of treating CHF, significantly increase left ventricular ejection fraction and 6-minute walk distance, and remarkably reduce brain natriuretic peptide. ZYC, with definite efficacy and safety, has the value of clinical application and in-depth research.
摘要：
Atherosclerosis (AS) is the major form of cardiovascular disease and the leading cause of morbidity and mortality in countries around the world. Atherosclerosis combines the interactions of systemic risk factors, haemodynamic factors, and biological factors, in which biomechanical and biochemical cues strongly regulate the process of atherosclerosis. The development of atherosclerosis is directly related to hemodynamic disorders and is the most important parameter in the biomechanics of atherosclerosis. The complex blood flow in arteries forms rich WSS vectorial features, including the newly proposed WSS topological skeleton to identify and classify the WSS fixed points and manifolds in complex vascular geometries. The onset of plaque usually occurs in the low WSS area, and the plaque development alters the local WSS topography. low WSS promotes atherosclerosis, while high WSS prevents atherosclerosis. Upon further progression of plaques, high WSS is associated with the formation of vulnerable plaque phenotype. Different types of shear stress can lead to focal differences in plaque composition and to spatial variations in the susceptibility to plaque rupture, atherosclerosis progression and thrombus formation. WSS can potentially gain insight into the initial lesions of AS and the vulnerable phenotype that gradually develops over time. The characteristics of WSS are studied through computational fluid dynamics (CFD) modeling. With the continuous improvement of computer performance-cost ratio, WSS as one of the effective parameters for early diagnosis of atherosclerosis has become a reality and will be worth actively promoting in clinical practice. The research on the pathogenesis of atherosclerosis based on WSS is gradually an academic consensus. This article will comprehensively review the systemic risk factors, hemodynamics and biological factors involved in the formation of atherosclerosis, and combine the application of CFD in hemodynamics, focusing on the mechanism of WSS and the complex interactions between WSS and plaque biological factors. It is expected to lay a foundation for revealing the pathophysiological mechanisms related to abnormal WSS in the progression and transformation of human atherosclerotic plaques.
期刊：
Frontiers in Microbiology,2023年14:1208157 ISSN：1664-302X
通讯作者：
Song, HP;Zeng, Meiyan
作者机构：
[Song, Houpan; Yuan, Chengzhi; Xiong, Meng; Sun, Qifang; Yu, Chang] Hunan Univ Chinese Med, Hunan Prov Key Lab Tradit Chinese Med Diagnost, Changsha, Hunan, Peoples R China.;[Yuan, Chengzhi] Hunan Univ Chinese Med, Sch Med, Changsha, Hunan, Peoples R China.;[Zeng, Meiyan; Song, Houpan; Xiong, Meng; Sun, Qifang; Yu, Chang] Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Hunan, Peoples R China.;[Zhou, Sainan] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.
通讯机构：
[Zeng, MY; Song, HP ] H;Hunan Univ Chinese Med, Hunan Prov Key Lab Tradit Chinese Med Diagnost, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Hunan, Peoples R China.
摘要：
Resistance of Helicobacter pylori (H. pylori) to antibiotics has reached alarming levels worldwide, and the efficacy of the H. pylori eradication treatment has decreased dramatically because of antibiotic resistance. To gain a more comprehensive understanding of the development status, research hotspots, and future trends related to H. pylori antibiotic resistance, we conducted a thorough retrospective analysis via the bibliometrics method. We searched the Science Citation Index Expanded of the Web of Science Core Collection for all pertinent articles on H. pylori antibiotic resistance from 2013 to 2022. R-bibliometrix, CiteSpace, and VOSviewer tools were utilized to depict statistical evaluations in order to provide an unbiased presentation and forecasts in the field. We incorporated a total of 3,509 articles related to H. pylori antibiotic resistance. Publications were inconsistent prior to 2017, but steadily increased after 2017. China generated the most papers and the United States of America received the most citations and the highest H-index. Baylor College of Medicine was the most influential institution in this field, with the highest number of publications and citations, as well as the highest H-index. Helicobacter was the most productive journal, followed by the World Journal of Gastroenterology and Frontiers in Microbiology. The World Journal of Gastroenterology had the highest citation. Graham, David Y was the most productive and cited author. Clarithromycin resistance, prevalence, gastric cancer, quadruple therapy, sequential therapy, 23S rRNA, whole genome sequencing, bismuth, and probiotics appeared with a high frequency in the keywords. The top keywords with the highest citation bursts were vonoprazan, RdxA, biofilm formation, and fatty acid chain. Our research illustrated a multi-dimensional facet and a holistic knowledge structure for H. pylori antibiotic resistance research over the past decade, which can serve as a guide for the H. pylori research community to conduct in-depth investigations in the future.
通讯作者：
Yi Zhang<&wdkj&>Jun Jiang<&wdkj&>Yi Zhang Yi Zhang Yi Zhang<&wdkj&>Jun Jiang Jun Jiang Jun Jiang
作者机构：
[Yi Sun; Yi Sun Yi Sun Yi Sun] Department of Breast and Thyroid Surgery, Xuchang Central Hospital, Xuchang, Henan, China;[Jinhui Hu; Jinhui Hu Jinhui Hu Jinhui Hu] Department of Breast Surgery, The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, China;[Yanwu Zhang; Yanwu Zhang Yanwu Zhang Yanwu Zhang] Department of Breast Surgery, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China;[Xiaowei Qi; Peng Tang; Linjun Fan; Li Chen; Shushu Wang; Yan Liang; Ying Hu; Minghao Wang; Lin Ren; Guozhi Zhang; Xuanni Tan; Long Yuan; Junze Du; Xiujuan Wu; Yi Zhang; Jun Jiang; Xiaowei Qi Xiaowei Qi Xiaowei Qi; Peng Tang Peng Tang Peng Tang; Linjun Fan Linjun Fan Linjun Fan; Li Chen Li Chen Li Chen; Shushu Wang Shushu Wang Shushu Wang; Yan Liang Yan Liang Yan Liang; Ying Hu Ying Hu Ying Hu; Minghao Wang Minghao Wang Minghao Wang; Lin Ren Lin Ren Lin Ren; Guozhi Zhang Guozhi Zhang Guozhi Zhang; Xuanni Tan Xuanni Tan Xuanni Tan; Long Yuan Long Yuan Long Yuan; Junze Du Junze Du Junze Du; Xiujuan Wu Xiujuan Wu Xiujuan Wu; Yi Zhang Yi Zhang Yi Zhang; Jun Jiang Jun Jiang Jun Jiang] Department of Breast and Thyroid Surgery, Southwest Hospital, Army Medical University, Chongqing, China;The Eighth Medical Center of Chinese PLA General Hospital, Beijing, China
通讯机构：
[Yi Zhang; Jun Jiang; Yi Zhang Yi Zhang Yi Zhang; Jun Jiang Jun Jiang Jun Jiang] D;Department of Breast and Thyroid Surgery, Southwest Hospital, Army Medical University, Chongqing, China
关键词：
HER2‐positive breast cancer;multicenter study;neoadjuvant therapy;pyrotinib;trastuzumab
摘要：
In this multicenter, single‐arm trial, we investigated the efficacy and safety of the ECPy‐THPy regimen (epirubicin, cyclophosphamide, and pyrotinib followed by docetaxel, trastuzumab, and pyrotinib) as neoadjuvant therapy for patients with stage II–III HER2‐positive breast cancer. Our results revealed that tpCR was achieved in 107 (68.6%) out of 156 patients, which was in line with our previous pilot study and numerically higher than the tpCR rate in previous phase III KRISTINE trial and the phase III POENY trial. Abstract The objective of this multicenter, single‐arm trial (ChiCTR1900022293) was to explore the efficacy and safety of neoadjuvant therapy with epirubicin, cyclophosphamide, and pyrotinib followed by docetaxel, trastuzumab, and pyrotinib (ECPy‐THPy) in the treatment of patients with stage II–III HER2‐positive breast cancer. The present study enrolled patients with stage II–III HER2‐positive breast cancer. Epirubicin and cyclophosphamide were administrated for four 21‐day cycles, followed by four cycles of docetaxel and trastuzumab. Pyrotinib was taken orally once per day throughout the treatment period. The primary endpoint was total pathological complete response (tpCR, ypT0/is ypN0) rate in the modified intention‐to‐treat (mITT) population. In total, 175 patients were included. The tpCR rate was 68.6% (95% CI, 60.7–75.8%), while the objective response rate was 89.1%. In the post‐hoc subgroup analysis, no association between clinical characteristics and the tpCR rate was observed. The most common grade ≥3 adverse events were diarrhea (54.3%), followed by white blood cell count decreased (5.1%), and neutrophil count decreased (4.6%). In conclusion, the neoadjuvant regimen with ECPy‐THPy showed promising pathological response and clinical benefits with an acceptable safety profile in patients with stage II–III HER2‐positive breast cancer.
通讯机构：
[Shi, LR ] ;Cent South Univ, Xiangya Hosp, Dept Radiol, 87 Xiangya Rd, Changsha 410005, Peoples R China.
摘要：
PURPOSE: To assess the safety and efficacy of local ablation plus PD-1 inhibitor toripalimab in previously treated unresectable hepatocellular carcinoma (HCC). PATIENTS AND METHODS: In the multicenter, two-stage, and randomized phase 1/2 trial, patients were randomly assigned to receive toripalimab alone (240 mg, every 3 weeks), subtotal local ablation followed by toripalimab starting on post-ablation day 3 (Schedule D3), or on post-ablation day 14 (Schedule D14). The first endpoint of stage 1 was to determine which combination schedule could continue and progression-free survival (PFS) as the primary endpoint for stage 1/2. RESULTS: A total of 146 patients were recruited. During stage 1, Schedule D3 achieved numerically higher objective response rate (ORR) than Schedule D14 for non-ablation lesions (37.5% vs. 31.3%), and was chosen for stage 2 evaluation. For the entire cohort of both stages, patients with Schedule D3 had a significantly higher ORR than with toripalimab alone (33.8% vs. 16.9%; P = 0.027). Moreover, patients with Schedule D3 had improved median PFS (7.1 vs. 3.8 months; P < 0.001) and median overall survival (18.4 vs. 13.2 months; P = 0.005), as compared with toripalimab alone. In addition, six (9%) patients with toripalimab, eight (12%) with Schedule D3, and 4 (25%) with Schedule D14 developed grade 3 or 4 adverse events, and one patient (2%) with Schedule D3 manifested grade 5 treatment-related pneumonitis. CONCLUSIONS: In patients with previously treated unresectable HCC, subtotal ablation plus toripalimab improved the clinical efficacy as compared with toripalimab alone, with an acceptable safety profile.
摘要：
目的采用文献计量学方法分析口腔黏膜下纤维性变(OSF)的研究现状和热点。方法在Web of Science(WOS)核心集中搜索OSF相关文献,检索时限均从建库至2022年12月31日。综合运用CiteSpace软件和V...展开更多 目的采用文献计量学方法分析口腔黏膜下纤维性变(OSF)的研究现状和热点。方法在Web of Science(WOS)核心集中搜索OSF相关文献,检索时限均从建库至2022年12月31日。综合运用CiteSpace软件和VOSviewer软件对作者、国家分布、期刊共引等内容进行知识图谱绘制,并进行可视化分析。结果共纳入1530篇文献,文献年发表量呈稳定增长的趋势;OSF相关研究热点包括口腔癌、黏膜白斑、槟榔、槟榔碱、烟草以及黏膜下纤维变性。结论文献可视化分析直观地展现了OSF相关研究的热点、前沿、核心期刊和核心作者,为相关学者的学习与研究提供了参考。收起
摘要：
Liuwei Dihuang Pill (LP) was verified to alleviate postmenopausal osteoporosis (PMOP) development. Nevertheless, the major constituent of LP and the related network pharmacology study remain unexplored. Protein–protein interaction was established to identify the downstream target of LP in PMOP, and the related signaling pathway was investigated by bioinformatics analysis. MC3T3-E1 cells were added to ferric ammonium citrate (FAC) to mimic osteoporosis in vitro. The osteoblasts were identified by Alizarin red staining. Western blot was applied to evaluate protein levels. In addition, Cell Counting Kit-8 (CCK8) assay was applied to assess cell viability, and cell apoptosis was assessed by flow cytometry. Quercetin was the major constituent of LP. In addition, quercetin significantly reversed FAC-induced inhibition of osteogenic differentiation in MC3T3-E1 cells. In addition, quercetin notably abolished the FAC-induced upregulation of Bax, Caspase-3, FOS, JUN, TGFB1 and PPARD. In contrast, Bcl-2, p-mTOR/mTOR, p-AKT/AKT and p-PI3K/PI3K levels in MC3T3-E1 cells were reduced by FAC, which was restored by quercetin. Meanwhile, FAC notably inhibited the viability of MC3T3-E1 cells via inducing apoptosis, but this impact was abolished by quercetin. Furthermore, quercetin could reverse pcDNA3.1-FOS-mediated growth of FAC-treated osteoblasts by mediating PI3K/AKT/mTOR signaling. Quercetin alleviated the progression of PMOP via activation of PI3K/AKT/mTOR signaling. Hence, this study would shed novel insights into discovering new methods against PMOP.
通讯机构：
[Zhou, J.; Ren, F.; Zhu, L.] D;[Huang, G.] H;Hernia and Abdominal Wall Surgery Center, China;Department of Gastrointestinal Hernia Surgery, China;Department of Geriatric Surgery, China
期刊：
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS,2023年37(3):1381-1391 ISSN：0393-974X
通讯作者：
Chen, XY;Cai, HZ
作者机构：
[Deng, Xu; Chen, Xinyu; Xu, Zelin] Hunan Univ Tradit Chinese Med, Hosp 1, Dept Prevent & Treatment Ctr, Changsha 410007, Hunan, Peoples R China.;[Luo, Yuntao] Hunan Univ Tradit Chinese Med, Hosp 1, Dept Hlth Management, Changsha 410007, Hunan, Peoples R China.;[Cai, Huzhi] Hunan Univ Tradit Chinese Med, Hosp 1, Dept Int Med, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Chen, XY ; Cai, HZ ] H;Hunan Univ Tradit Chinese Med, Hosp 1, Dept Prevent & Treatment Ctr, Changsha 410007, Hunan, Peoples R China.;Hunan Univ Tradit Chinese Med, Hosp 1, Dept Int Med, Changsha 410007, Hunan, Peoples R China.
关键词：
Notch signal pathway;miR-18a-5p;chronic heart failure;cardiac function;cardiomyocyte;apoptosis
摘要：
Objectives: To explore how the mechanism of miR-18a-5p overexpression through the Notch signaling pathway improves cardiac function and inhibits cardiomyocyte apoptosis in chronic heart failure rats. Methods: Forty rats were randomly divided into contrast group, negative control (NC) group, high-expression group, and low -expression group by random number table, with ten rats in each group. NC group rats, high-expression group rats, and low -expression group rats were injected intraperitoneally with 2 mg/mL adriamycin solution to establish the rat model of chronic heart failure. The miR-18a-5p overexpression and inhibition lentivirus vector was constructed, and the miR-18a-5p overexpression and inhibition lentivirus suspension were injected into the myocardium of rats in the high-expression group and low-expression group respectively. No treatment was made in the contrast group and NC group. The expression levels of miR-18a-5p were detected in the myocardial tissue of rats and the cardiac function of rats. In addition, the apoptosis of rat cardiomyocytes was observed, and the expression levels of apoptosis-related proteins (Cleaved-caspase-3, Bax, and Bcl-2) were detected in the myocardial tissue of rats. The possible binding sites of miR-18a-5p and Notch2 3 ' untranslated region (UTR) region were predicted, and the interaction between miR-18a-5p and Notch2 was verified. Finally, the expression levels of Notch signal pathway-related genes and proteins (Notch2, Hes1, and Hes5) were detected in the myocardial tissue of rats. Results: In comparison with the NC group, miR-18a-5p expression levels in myocardial tissue of high-expression group rats increased (p < 0.05), and that of low-expression group rats decreased (p < 0.05). In contrast with the NC group and low-expression group, the values of left ventricular end-diastolic dimension (LVEDD) and left ventricular end-systolic dimension (LVESD) in the high-expression group decreased (p < 0.05), and the values of left ventricular ejection fraction (LVEF) and fraction shortening (FS) of the left ventricle in the high-expression group increased (p < 0.05). In comparison with the NC group and low-expression group, myocardial cell apoptosis in the high-expression group decreased (p < 0.05), and the protein expression levels of Cleaved-caspase-3 and Bax in myocardial tissue of high-expression group rats decreased (p < 0.05). Moreover, the Bcl-2 protein expression levels in the myocardial tissue of high-expression group rats increased (p < 0.05). Through prediction and verification, miR-18a-5p might bind to the "GCACCUUA" site of the Notch2 3 ' UTR region, which could inhibit the activity of wild-type Notch2 3 ' UTR luciferase reporter plasmid but could not inhibit the activity of mutant Notch2 3 ' UTR luciferase reporter plasmid. In comparison with the NC group and low-expression group, the messenger ribonucleic acid (mRNA) and protein expression levels of Notch signal pathway-related factors Notch2, Hes1, and Hes5 in myocardial tissue of high-expression group rats were decreased (p < 0.05).Conclusions: miR-18a-5p overexpression could improve cardiac function and inhibit cardiomyocyte apoptosis in chronic heart failure rats, which might be related to the regulation of the Notch signal pathway.
