摘要：
Objective: To explore the effects of Compound Qinbo Decoction (CQD) on the intestinal mucosa of ulcerative colitis (UC) through the Notch signaling pathway.Methods: The UC rat model, established using 2,4,6- Trinitrobenzene sulfonic acid (TNBS)/ethanol, was divided into the UC group, mesalazine (MS) group and CQD group. Normal rats were set as the normal group (n = 10). The pathological changes, messager RNA (mRNA) levels and protein expressions of Notch 1 and hairy and enhancer of split 1 (Hes1), epithelial cell proliferation in colon tissues were detected. Primary rat colon epithelial NCM460 cells were added with Tumor Necrosis Factor-a (TNF-a) to establish the UC model, then treated with different drug-contained serum. The cell proliferation and protein expressions of Notch 1 and Hes1 were measured.Results: In vivo, the UC rat model was established with obvious pathological changes in colon tissues, which were greatly relieved by MS or CQD. After MS or CQD treatment, mRNA and protein levels of Notch 1 and Hes1 in the UC rat model were increased (p < 0.05). The epithelial cell proliferation was listed from high to low: MS group, CQD group, normal group and UC group (p < 0.05). In vitro, UC in NCM460 cells was established with TNF-a of 50 ng/mL for 6 h, and the cell proliferation level was decreased, which was then increased with MS-containing serum or CQD-containing serum (p < 0.05). The protein expressions of Notch 1 and Hes1 were decreased with TNF-a treatment, which was increased with MS-containing serum or CQD-containing serum (p < 0.05). The effect of CQD-containing serum was suppressed by the Notch signaling pathway inhibitor (p < 0.05).Conclusions: CQD has a good therapeutic effect on the intestinal mucosa of UC through the Notch signaling pathway, and the underlying mechanism needs to be explored in the future.
作者机构：
[Huang, Pan; Li, Ying; Peng, Youhua; Zeng, Bijun; Zhang, Yujin; Wang, Haizhen; Yang, Zhibo; Luo, Meijunzi] Hunan Univ Chinese Med, Hosp 2, Dept Dermatol, Domest Class Discipline Construct Project Chinese, Changsha 410005, Hunan, Peoples R China.;[Tang, Qing; Gao, Jie] Hunan Univ Chinese Med, Grad Sch, Changsha 410005, Hunan, Peoples R China.;[Gao, Jie] Ninth Hosp Changsha, Dept Dermatol, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Bijun Zeng; Zhibo Yang] D;Department of Dermatology, The Second Hospital of Hunan University of Chinese Medicine, The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, Changsha, China<&wdkj&>Department of Dermatology, The Second Hospital of Hunan University of Chinese Medicine, The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, Changsha, China
摘要：
BACKGROUND: Androgenetic alopecia can affect up to 70% of males and 40% of females; however, certain therapeutic medications offer partial and transitory improvement but with major side effects. Dendrobium officinale polysaccharide (DOP) has been reported to improve androgen-related hair loss in mice, but the molecular mechanism remains unclear. OBJECTIVES: To explore the effects of DOP on androgenetic alopecia. METHODS: In this study, testosterone was subcutaneously administered to shave dorsa skin of mice to establish androgenetic alopecia; the effects of DOP in androgenetic alopecia were explored by DOP administration. RESULTS: Testosterone treatment extended the time of skin growing dark and hair growing, decreased the mean numbers of follicles in skin tissues, decreased β-catenin and cyclin D1 levels, and elevated testosterone, DHT (dihydrotestosterone), and 5α-reductase levels. In contrast, DOP administration shortened skin growing dark and hair growing times, promoted follicle cell proliferation, increased follicle numbers, increased β-catenin and cyclin D1 levels, and decreased testosterone, DHT, and 5α-reductase levels. CONCLUSION: DOP application significantly improved testosterone-induced hair follicle miniaturization and hair loss, possibly through affecting the Wnt signaling and hair follicle stem cell functions. NO LEVEL ASSIGNED: This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
摘要：
Thirteen robustaflavones (1-13) were isolated from the 70% EtOH extract of Selaginella trichoclada Alston herbs for the first time. The structures of these compounds were identified on the basis of extensive spectroscopic data including UV-vis, 1D and 2D NMR, and mass spectra. The molecular docking results indicate that the robustaflavone derivates may play a role as a NEDD4 protein inhibitor.
摘要：
Immune-mediated inflammatory diseases (IMIDs) consist of a common and clinically diverse group of diseases. Despite remarkable progress in the past two decades, no remission is observed in a large number of patients, and no effective treatments have been developed to prevent organ and tissue damage. Brain-derived neurotrophic factor precursor (proBDNF) and receptors, such as p75 neurotrophin receptor (p75(NTR)) and sortilin, have been proposed to mediate intracellular metabolism and mitochondrial function to regulate the progression of several IMIDs. Here, the regulatory role of proBDNF and its receptors in seven typical IMIDs, including multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus, allergic asthma, type I diabetes, vasculitis, and inflammatory bowel diseases, was investigated.
期刊：
Skin Research and Technology,2023年29(9) ISSN：0909-752X
通讯作者：
Li, X
作者机构：
[Li, Xiaosha; Wang, Haizhen] Hunan Univ Chinese Med, Affiliated Hosp 2, Dept Dermatol, Changsha, Peoples R China.;[Tang, Shiyang] Hunan Univ Chinese Med, Sch Chinese Med, Changsha, Peoples R China.;[Li, Xin] Hunan Univ Chinese Med, Hunan Prov Key Lab Diagnost Chinese Med, 300 Xueshi Rd, Hanpu Sci & Educ Pk, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Li, X ] H;Hunan Univ Chinese Med, Hunan Prov Key Lab Diagnost Chinese Med, 300 Xueshi Rd, Hanpu Sci & Educ Pk, Changsha 410208, Hunan, Peoples R China.
关键词：
NLRP3;pyroptosis;ROS;tanshinoneIIA;vitiligo
摘要：
Abstract Background Pyroptosis has been implicated in the development of human diseases, including vitiligo. TanshinoneIIA has been confirmed to play anti‐vitiligo role. However, whether tanshinoneIIA inhibits vitiligo progression via regulating cell pyroptosis remains unclear. Methods Hydrogen peroxide (H2O2)‐induced melanocytes were used to mimic vitiligo cell model in vitro. Cell viability was assessed by cell counting kit 8 assay, and reactive oxygen species (ROS) production was detected by DCFH‐DA staining. Nod‐like receptor protein 3 (NLRP3) expression was detected by quantitative real‐time PCR, western blot and immunofluorescence staining. Cell pyroptosis was measured using flow cytometry, and the contents of interleukin‐1β and interleukin‐18 were determined by ELISA. Besides, the protein levels of apoptosis‐associated speck‐like protein containing CARD (ASC) and cleaved‐Caspase‐1 were examined by western blot analysis. Results H2O2 could induce ROS production, NLRP3 expression and pyroptosis in melanocytes. TanshinoneIIA inhibited ROS production, pyroptosis, and the expression of NLRP3, ASC and cleaved‐caspase‐1 in H2O2‐induced melanocytes. Compared with the function of ROS inhibitor (NAC), tanshinoneIIA acted as a ROS scavenger to relieve melanocyte pyroptosis. In addition, NLRP3 inhibitor (MCC950) also could aggravate the inhibition effect of tanshinoneIIA on melanocyte pyroptosis. Conclusion TanshinoneIIA suppressed melanocyte pyroptosis probably through modulating the ROS/NLRP3 signaling axis, which provides the evidence for therapeutic effect in vitiligo.
期刊：
Journal of Ethnopharmacology,2023年307:116203 ISSN：0378-8741
通讯作者：
Xiaolong Lu
作者机构：
[Lu, Xiaolong] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Orthoped, Changsha 410005, Hunan, Peoples R China.;[Lu, Xuedi; Li, Wei; Li, Juan; Ouyang, Jian] Hunan Univ Chinese Med, Affiliated Hosp 2, Dept Orthoped, Changsha 410005, Hunan, Peoples R China.;[Zhou, Biao] Chinese Acad Chinese Med Sci, Wangjing Hosp, Dept Orthoped, Beijing 100102, Peoples R China.;[Zhou, Biao] Univ South China, Xiangtan Hosp, Dept Orthoped, Xiangtan 411101, Hunan, Peoples R China.;[Lu, Xiaolong] 233 Cai E Bei Rd, Changsha 410005, Hunan, Peoples R China.
通讯机构：
[Xiaolong Lu] D;Department of Orthopedics, Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410005, Hunan Province, PR China
摘要：
ETHNOPHARMACOLOGICAL RELEVANCE: Taohong Siwu Decoction (THSWD) is a conventional traditional Chinese prescription aiming at promoting blood circulation and alleviating blood stasis. It is widely prescribed in instances of ischemic strokes, cardiovascular diseases, osteoporosis and bone fracture. However, its molecular functions in bone formation remain uncharacterized. AIM OF STUDY: This study aims to explore the potential effects of THSWD treatment on human bone marrow mesenchymal stem cells (BMSCs) proliferation, osteogenic differentiation, and migration. MATERIALS AND METHODS: BMSCs undergo osteogenic, adipogenic, and chondrogenic differentiation to determine cell stemness. BMSCs were treated with low dose (200μg/ml), medium dose (400μg/ml) and high dose (600μg/ml) THSWD. The cell viability was determined by CCK-8 assays, the osteogenic differentiation ability was determined by alizarin red staining and ALP staining, and cell migration was determined by wound healing and transwell assays. The effect of THSWD on the vascular endothelial growth factor (VEGF)/focal adhesion kinase (FAK) pathway was determined by immunoblotting. RESULTS: THSWD time-dependently and dose-dependently promoted BMSC viability. Moreover, THSWD also promoted BMSC osteogenic differentiation and migration. As opposed to THSWD, VEGF receptor inhibitor Bevacizumab suppressed BMSC osteogenic differentiation and migration. In BMSCs that have been co-treated with THSWD and Bevacizumab, THSWD effects on BMSC functions were partially eliminated by Bevacizumab. Moreover, THSWD treatment boosted VEGF content in the supernatant and was conducive to the phosphorylation of FAK and Src, whereas Bevacizumab exerted opposite effects; similarly, Bevacizumab partially abolished THSWD effects on VEGF and FAK (Tyr397) and Src (Tyr418) phosphorylation. CONCLUSION: THSWD enhances the capacities of BMSCs to proliferate, differentiate, and migrate, possibly through VEGF and the FAK-Src, thereby improving fracture healing.
摘要：
Objective: Exploring the feasibility of combining herbal fumigation and myoelectric biofeedback therapy in football players with post-stroke shoulder-hand syndrome. Methods: A total of 80 football players with shoulder-hand syndrome after stroke who were healed in our hospital from July 2019 to June 2021 were retrospectively opted as the research subjects, and were divided into a joint intervention cluster (JIG) according to the variations in their healing methods. cluster, n=40, receiving traditional Chinese medicine fumigation and EMG biofeedback healing) and EMG healing cluster (Electromyobiological feedback cluster, EFG cluster, n=40), the healing effect, changes in simplified FMA mark of upper limbs before and after healing, and healing effects were contrasted between the two clusters. The changes of the front and rear shoulder pain and the pain part of the High Coast Shoulder Joint Function Rating Scale were recorded, and the occurrence of adverse reactions in the two clusters of football players was recorded; Results: The total effective rate of football players in the JIG cluster was 97.50% (39/40), which was notably upper than 85.00% (34/40) in the EFG cluster, and the variation between the clusters was notable (P<0.05). None notable variation in the simplified FMA mark between the clusters (P>0.05). On the 7th, 14th, and 28th days of healing, the simplified FMA mark of the upper limbs of the JIG cluster was notably upper than that of the EFG cluster, and the variation was notable (P>0.05). P<0.05); before healing, None notable variation between the two clusters in the degree of shoulder pain and the pain part of the Gaoshore Shoulder Joint Function Assessment Scale (P>0.05). After 28 days of healing, the degree of shoulder pain in the JIG cluster was notably bottom In the EFG cluster, the pain mark of the Gaoan Shoulder Joint Function Assessment Scale was notably upper than that in the EFG cluster, and the variation between the two clusters was notable (P<0.05); None notable variation in the incidence of adverse reactions between the two clusters (P >0.05); Conclusion: Combination of traditional Chinese medicine fumigation and EMG biofeedback for football players with shoulder-hand syndrome after stroke has a good effect, which can notably relieve the pain symptoms of football players, enhance their joint mobility, and at the same time, the healing is safe.
作者机构：
The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, China;Zhangjiajie Hospital of Traditional Chinese Medicine, Hunan Province, Zhangjiajie, China;[XING Bowen; LIU Weiai; WU Pingxiang; QIN Simin; JIANG Yu; ZHAN Lifen; XIE Yunfang; HE Ke] The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410005,China;[SHI Haibing] Zhangjiajie Hospital of Traditional Chinese Medicine,Hunan Province,Zhangjiajie 427000,China
通讯机构：
[Ke He; Weiai Liu] T;The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, China<&wdkj&>The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, China
摘要：
Objective:To investigate the effects and the possible mechanisms of Neiguan(PC6)and Gongsun(SP4)on the hypothalamic-pituitary-adrenal(HPA)axis in rats with functional dyspepsia(FD),thus to provide a theoretical basis for the clinical application of the Eight Confluent P...MORE Objective:To investigate the effects and the possible mechanisms of Neiguan(PC6)and Gongsun(SP4)on the hypothalamic-pituitary-adrenal(HPA)axis in rats with functional dyspepsia(FD),thus to provide a theoretical basis for the clinical application of the Eight Confluent Points.Methods:Forty specific-pathogen-free Sprague-Dawley rats were divided into a blank group,a model group,an electroacupuncture(EA)group,and a Western medicine group by the random number table method,with 10 rats in each group.Rats in the blank group did not receive modeling or intervention.Rats in the other three groups were subjected to the FD with mood disorder model using the compound etiology modeling method.After the successful modeling,rats in the model group did not receive any interventions,rats in the Western medicine group received deanxit and mosaprid intervention,and those in the EA group received EA intervention on the ipsilateral Neiguan(PC6)and Gongsun(SP4)for 21 d.The sugar-water consumption rate was measured before the experiment and before and after interventions to assess the emotional status.The gastric emptying rate was measured after interventions to assess the gastrointestinal dynamics.The expression levels of hypothalamic corticotropin-releasing hormone(CRH),pituitary adrenocorticotropic hormone(ACTH),and adrenal corticosterone(CORT)were measured by enzyme-linked immunosorbent assay.Results:Compared with the blank group,the sugar-water consumption rate and the gastric emptying rate were decreased(P<0.01),and the hypothalamic CRH,pituitary ACTH,and adrenal CORT expression levels were increased(P<0.01)in the model group.Compared with the model group,the sugar-water consumption rate and the gastric emptying rate were significantly increased(P<0.01),while the expression levels of hypothalamic CRH,pituitary ACTH,and adrenal CORT were significantly decreased(P<0.01)in the EA group and the Western medicine group.The differences between the EA group and the Western medicine group were not statistically significant(P>0.05).Conclusion:The Eight Confluent Points Neiguan(PC6)and Gongsun(SP4)can improve the mood and gastrointestinal dynamics in FD rats,which may be achieved by down-regulating the hypothalamic CRH,pituitary ACTH,and adrenal CORT,as well as by correcting the HPA axis hyperfunction.FEWER
作者机构：
[李恋秋; 张鹏; 陈壮志; 林佩怡; 许慧; 康贞] Department of Acupuncture-Moxibustion, Tuina and Rehabilitation, Second Affiliated Hospital of Hunan University of CM, Changsha 410005, China;[方灵莨; 叶海敏] Neurological Department, Second Affiliated Hospital of Hunan University of CM, Changsha 410005, China
作者机构：
[Zhang, Yi; Zhang, Y] Hunan Univ Tradit Chinese Med, Affiliated Hosp 2, Dept Gynecol, Changsha 410005, Peoples R China.;[Wu, Lulu] Huazhong Univ Sci & Technol, Cent Hosp Wuhan, Tongji Med Coll, Dept Integrated Tradit Chinese & Western Med, Wuhan 430014, Peoples R China.;[Wen, Xiang] First Peoples Hosp Huizhou City, Dept Pathol, Huizhou 516000, Peoples R China.;[Lv, Xiuwei] Rocket Force Med Ctr PLA, Dept Tradit Chinese Med, Beijing 100088, Peoples R China.
通讯机构：
[Zhang, Y ] H;Hunan Univ Tradit Chinese Med, Affiliated Hosp 2, Dept Gynecol, Changsha 410005, Peoples R China.
关键词：
Endometriosis;Gene set variation analysis;Gene set;Diagnostic biomarker
摘要：
Objective: The enigmatic nature of Endometriosis (EMS) pathogenesis necessitates investigating alterations in signaling pathway activity to enhance our comprehension of the disease's characteristics.Methods: Three published gene expression profiles (GSE11691, GSE25628, and GSE7305 datasets) were downloaded, and the "combat" algorithm was employed for batch correction, gene expression difference analysis, and pathway enrichment difference analysis. The protein-protein interaction (PPI) network was constructed to identify core genes, and the relative enrichment degree of gene sets was evaluated. The Lasso regression model identified candidate gene sets with diagnostic value, and a risk scoring diagnostic model was constructed for further validation on the GSE86534 and GSE5108 datasets. CIBERSORT was used to assess the composition of immune cells in EMS, and the correlation between EMS diagnostic value gene sets and immune cells was evaluated.Results: A total of 568 differentially expressed genes were identified between eutopic and ectopic endometrium, with 10 core genes in the PPI network associated with cell cycle regulation. Inflammation-related pathways, including cytokine-receptor signaling and chemokine signaling pathways, were significantly more active in ectopic endometrium compared to eutopic endometrium. Diagnostic gene sets for EMS, such as homologous recombination, base excision repair, DNA replication, P53 signaling pathway, adherens junction, and SNARE interactions in vesicular transport, were identified. The risk score's area under the curve (AUC) was 0.854, as indicated by the receiver operating characteristic (ROC) curve, and the risk score's diagnostic value was validated by the validation cohort. Immune cell infiltration analysis revealed correlations between the risk score and Macrophages M2, Plasma cells, resting NK cells, activated NK cells, and regulatory T cells.Conclusion: The risk scoring diagnostic model, based on pathway activity, demonstrates high diagnostic value and offers novel insights and strategies for the clinical diagnosis and treatment of Endometriosis.
