期刊：
Frontiers in Pharmacology,2023年14:1173747 ISSN：1663-9812
通讯作者：
Long, HP
作者机构：
[Zhou, Siqian; Long, HP; Liu, Jian; Long, Hongping] Hunan Univ Chinese Med, Ctr Med Res & Innovat, Hosp 1, Changsha, Peoples R China.;[Ding, Changsong; Zhou, Siqian; Wang, Yajing; Long, HP; Long, Hongping] Hunan Univ Chinese Med, Changsha, Peoples R China.;[Tan, Leihong] Hunan Univ Chinese Med, Dept Pharm, Hosp 2, Changsha, Peoples R China.;[Wang, Yikun] Cent South Univ, Xiangya Hosp 2, Dept Pharm, Changsha, Peoples R China.;[Li, Jing] Cent South Univ, Xiangya Hosp, Dept Pharm, Changsha, Peoples R China.
通讯机构：
[Long, HP ] H;Hunan Univ Chinese Med, Ctr Med Res & Innovat, Hosp 1, Changsha, Peoples R China.;Hunan Univ Chinese Med, Changsha, Peoples R China.
摘要：
Introduction: Corni Fructus (CF) is a Chinese herbal medicine used for medicinal and dietary purposes. It is available commercially in two main forms: raw CF (unprocessed CF) and wine-processed CF. Clinical observations have indicated that wine-processed CF exhibits superior hypoglycemic activity compared to its raw counterpart. However, the mechanisms responsible for this improvement are not well understood. Methods: To address this gap in knowledge, we conducted metabolomics analysis using ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-QTOF-MS) to compare the chemical composition of raw CF and wine-processed CF. Subsequently, network analysis, along with immunofluorescence assays, was employed to elucidate the potential targets and mechanisms underlying the hypoglycemic effects of metabolites in CF. Results: Our results revealed significant compositional differences between raw CF and wine-processed CF, identifying 34 potential markers for distinguishing between the two forms of CF. Notably, wine processing led to a marked decrease in iridoid glycosides and flavonoid glycosides, which are abundant in raw CF. Network analysis predictions provided clues that eight compounds might serve as hypoglycemic metabolites of CF, and glucokinase (GCK) and adenylate cyclase (ADCYs) were speculated as possible key targets responsible for the hypoglycemic effects of CF. Immunofluorescence assays confirmed that oleanolic acid and ursolic acid, two bioactive compounds present in CF, significantly upregulated the expression of GCK and ADCYs in the HepG2 cell model. Discussion: These findings support the notion that CF exerted hypoglycemic activity via multiple components and targets, shedding light on the impact of processing methods on the chemical composition and hypoglycemic activity of Chinese herbal medicine.
作者机构：
[Liu, Yu] Hunan Univ Chinese Med, Coll Acupuncture & Moxibust & Tui Na, Changsha, Peoples R China.;[Gong, Houwu] DHC SOFTWARE CO LTD, DHC Mediway Technol Co Ltd, Beijing, Peoples R China.;[Chen, Heng] Hunan Univ Chinese Med, Affiliated Hosp 2, Dept Encephalopathy, Changsha, Peoples R China.
会议名称：
IEEE International Conference on Bioinformatics and Biomedicine (BIBM)
会议时间：
NOV 18-21, 2019
会议地点：
San Diego, CA
会议主办单位：
[Liu, Yu] Hunan Univ Chinese Med, Coll Acupuncture & Moxibust & Tui Na, Changsha, Peoples R China.^[Gong, Houwu] DHC SOFTWARE CO LTD, DHC Mediway Technol Co Ltd, Beijing, Peoples R China.^[Chen, Heng] Hunan Univ Chinese Med, Affiliated Hosp 2, Dept Encephalopathy, Changsha, Peoples R China.
会议论文集名称：
IEEE International Conference on Bioinformatics and Biomedicine-BIBM
关键词：
Xievin Decoction Analogous Prescriptions;Xinkai Kujiang therapy(herbs acrid in flavor for dispersing and herbs bitter in flavorfor descending);complex network;data mining
摘要：
Objective To summarize the core drugs, compatibility rules and properties, flavor and meridian tropism of the Xiexin Decoction Analogous Prescriptions by complex network mining techniques, in order to provide reference for the research of the Analogous prescriptions. Methods The prescription information was extracted from the case of Xiexin Decoction Analogous Prescription(herbs acrid in flavor for dispersing and herbs bitter in flavor for descending)in the the "Ancient and Modern Medical Case Cloud Platform", and the core drugs, compatibility rules and properties,flavor and meridian tropism of the Xiexin Decoction Analogous Prescriptions were analyzed. Results The core drugs of Xiexin Decoction Analogous Prescription were Pinellia, Dried Ginger, Licorice, Rhizoma Coptidis, Scutellaria, Poria, Codonopsis, Jujube, Radix Paeoniae Alba, Atractylodes Rhizome, Tangerine, Amomum vulgaris. The herbs especially belonged to the warm properties,the acrid flavor, and the meridian tropism of these drugs belonged to spleen and stomach channels. Excavation of the common drugs on the compatibility and network compatibility group are: Scutellaria-Coptis, Dried ginger - Pinellia, Jujube - Licorice - Codonopsis, Berberine+Scutellaria Pinellia+Dried ginger. Conclusion Using the complex network mining technology, the core drugs, compatibility drugs and properties, flavor and meridian tropism of the Xiexin Decoction Analogous Prescription were obtained, which played an exemplary role in further digging into the compatibility of the analogous prescriptions.
作者机构：
[Yang Cheng-xi] Hunan Univ Chinese Med, Clin Med Coll 2, Changsha 410005, Hunan, Peoples R China.;[Yang Zhi-an] Key Lab Struct & Vibrat Engn Tangshan, Tangshan 063000, Hebei, Peoples R China.
通讯机构：
[Yang Cheng-xi] H;Hunan Univ Chinese Med, Clin Med Coll 2, Changsha 410005, Hunan, Peoples R China.
会议名称：
2012 The 2nd International Conference on Biotechnology,Chemial and Materials Engineering(第二届生物、化工和材料工程国际会议(CBCME2012))
会议时间：
2012-12-28
会议地点：
厦门
会议主办单位：
[Yang Cheng-xi] Hunan Univ Chinese Med, Clin Med Coll 2, Changsha 410005, Hunan, Peoples R China.^[Yang Zhi-an] Key Lab Struct & Vibrat Engn Tangshan, Tangshan 063000, Hebei, Peoples R China.
会议论文集名称：
2012 The 2nd International Conference on Biotechnology,Chemial and Materials Engineering(第二届生物、化工和材料工程国际会议(CBCME2012))论文集
关键词：
bone injury;bone biomechanics;mechanics;differentiation;synthesizing
摘要：
The paper first summarizes the evolution of the differentiation and synthesizing of bone injury discipline and mechanics and then, with the application of material mechanics, mechanical vibration and dynamics in bone injury discipline, comes to the conclusion that differentiation is the prerequisite for disciplinary development and synthesizing is the inevitable trend or result of disciplinary development.
