作者机构：
[潘思安; 刘余; 袁菡钰; 李娟; 岳增辉; 汪少华; 薛晓] College of Acupuncture-Moxibustion, Tuina and Rehabilitation, Hunan University of CM, Changsha 410208, China;[薛晓] Department of TCM, First Affiliated Hospital of University of South China
作者机构：
[张泓; 熊丹; 谢菊英; Qiu, Rong-Rong; 邓畅; 谭洁; Deng, Chang; 徐雅钰] School of Acupuncture-Message and Rehabilitation, Hunan University of Chinese Medicine, Changsha 410208, China
作者机构：
[Shatong Z.; Xiaoyu L.; Tianhua Z.; Xing W.; Xiaojuan X.; Yan P.] College of Acupuncture and Moxibustion and Tui-na, Hunan University of Traditional Chinese Medicine, Changsha, 410208, China
通讯机构：
[Yan, P.] C;College of Acupuncture and Moxibustion and Tui-na, China
作者机构：
[来奕恬; 周竞颖; 丁攀婷] Graduate School of Hunan University of Chinese Medicine, Changsha 410208, China;[刘密; 李南; 张国山] College of Acupuncture-moxibustion-Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha 410208;[邱冉冉] The First Hospital of Hunan University of Chinese Medicine, Changsha 410007
作者机构：
The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, College of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha, China;College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China;[杨建文; 林亚平; 彭艳; 张程程; 陈海交; 魏星] The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, College of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha 410000, China;[曹建中] College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410000, China
通讯机构：
[Zhang Cheng-cheng; Peng Yan] T;The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, College of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha, China<&wdkj&>The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, College of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha, China
关键词：
Acupuncture Therapy;Electroacupuncture;Calcium-activated Chloride Channel;Interstitial Cells of Cajal;Diabetes Complications;Gastroparesis;Rats
摘要：
To investigate the mechanisms of electroacupuncture (EA) at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) in intervening diabetic gastroparesis (DGP) based on calcium-activated chloride channel. Forty Sprague-Dawley rats were randomly divided into four groups, including a normal control group (group A), a model group (group B), an EA group (group C) and a metoclopramide group (group D), with 10 rats in each group. A single intraperitoneal injection of 2% streptozotocin (STZ) combined with 8-week high-glucose high-fat diet was used to establish a DGP rat model. After intervention, gastrointestinal propulsive rate was observed; the expression level of transmembrane protein 16A (TMEM16A) was examined by immunohistochemistry; the Ca2+ concentration in interstitial cells of Cajal (ICCs) was detected by immunofluorescence; and whole-cell patch-clamp technique was applied to detect the current intensity of calcium-activated chloride channel (ICaCC) in ICCs in gastric antrum. After modeling, the blood glucose levels in group B, group C and group D were significantly increased compared with group A (all P<0.01); after intervention, compared with group B, the blood glucose levels in group C and group D were significantly decreased (P<0.05, P<0.01); the intra-group comparison of blood glucose level between after modeling and after intervention found significant difference only in group C (P<0.01). The gastrointestinal propulsive rates in group B, group C and group D were significantly different from that in group A (P<0.01 or P<0.05); the gastrointestinal propulsive rates were markedly higher in group C and group D than in group B (P<0.01, P<0.01). The expressions of TMEM16A in group B and group C were decreased compared with group A (P<0.01, P<0.05); the expressions of TMEM16A in group C and group D were increased compared with group B (P<0.01, P<0.05). The fluorescence intensity of Ca2+ was significantly lower in group B than in group A (P<0.01); the fluorescence intensity of Ca2+ was significantly higher in group C and group D than in group B (P<0.01, P<0.05). ICaCC in ICCs in group B was significantly decreased compared with group A; ICaCC in group C and group D were increased compared with group B. EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) can significantly improve gastrointestinal motility in DGP rats by up-regulating the ICaCC in ICCs.
摘要：
OBJECTIVE: To explore how baseline resting-state cerebral function predicts clinical outcomes of acupuncture treatment for migraine. METHODS: One hundred migraine patients and 46 healthy subjects were recruited. Patients were randomized into the acupuncture, sham acupuncture, and waiting list groups. Resting-state functional magnetic resonance imaging data were collected at baseline and after 1 month of longitudinal acupuncture treatments. Fractional amplitude of low-frequency fluctuations (fALFF) calculations were applied to explore the associations between baseline fALFF and changes in clinical variables in the acupuncture treatment group and the waiting list group. RESULTS: Compared with healthy subjects, migraine patients had lower fALFF in the left rostral ventromedial medulla, right thalamus, left amygdala, and right angular gyrus. Regression analyses revealed that baseline fALFF values in the left middle frontal gyrus, left superior temporal gyrus, and middle temporal gyrus were positively associated with headache intensity changes in the acupuncture treatment group, while baseline fALFF values in the bilateral lingual gyrus and cuneus were negatively associated with headache intensity changes in this group. CONCLUSION: The baseline fALFF values of brain regions associated with cognitive pain modulation, but not migraine severity, may predict future headache intensity improvement levels in migraine patients receiving acupuncture treatment. (C) 2020 JTCM. All rights reserved.
作者：
Liu Mi;Shen Jiacheng;Liu Caichun;Zhong Huan;Yang Qing;...
期刊：
中医杂志(英文版),2020年40(5):827-835 ISSN：0255-2922
通讯作者：
She Chang;Yu Shu
作者机构：
[Ma Mingzhu; Chang Xiaorong; Shu Wenna; Yang Qing; Zhong Huan; Liu Mi] Hunan Univ Chinese Med, Coll Acupuncture & Moxibust & Tui Na, Changsha 410208, Peoples R China.;[Liu Mi] Liuyang Hosp Chinese Med, Liuyang 410300, Peoples R China.;[Yang Zong-bao; Shen Jiacheng; Liu Caichun] Xiamen Univ, Dept Tradit Chinese Med, Dept Elect Sci, Xiamen 361005, Peoples R China.;[Dong Jiyang] Xiamen Univ, Dept Elect Sci, Fujian Prov Key Lab Plasma & Magnet Resonance, Xiamen 361005, Peoples R China.;[Yu Shu] City Univ Hong Kong, Hong Kong 999077, Peoples R China.
通讯机构：
[She Chang] C;[Yu Shu] T;Changsha Eighth Hosp, Changsha Hosp Tradit Chinese Med, Changsha 410002, Peoples R China.;Tat Chee Ave, Hong Kong 999077, Peoples R China.
关键词：
Gastritis, atrophic;Metabolomic;Moxibustion;Acupuncture;Proton magnetic resonance spectroscopy
摘要：
OBJECTIVE: To evaluate the effects of moxibustion and acupuncture of Zusanli (ST 36) and Zhongwan (CV 12) acupoints on chronic atrophic gastritis (CAG) in rats, and to study the mechanisms behind their actions. METHODS: Forty-four male Sprague-Dawley rats were induced with CAG by intragastric administration of 40% ethanol combined with free drinking of N-methyl-N'-nitro-N-nitrosoguanidine and irregular feeding for 12 weeks, followed by daily treatment with moxibustion or acupuncture for 2 weeks. Histopathologic examination, Western blotting of cytokines [epidermal growth factor (EGF), EGF receptor (EGFR), extracellular signal-regulated kinase (ERK), phosphorylated ERK (p-ERK)], and H-1 NMR-based metabolic profiling of gastric tissues were used to measure changes related to CAG modeling and treatment. RESULTS: Moxibustion and acupuncture at Zusanli (ST 36) and Zhongwan (CV 12) each relieved CAG-induced abnormalities in histopathology and cytokine expression of ERK and p-ERK. Only moxibustion treatment regulated the expression of EGF and EGFR. Metabolites that were increased in gastric tissue by CAG induction (alanine, nicotinamide adenine dinucleotide phosphate, uracil DNA glycosylase, lactate, glycerol and adenosine) were restored to normal levels after moxibustion treatment; acupuncture treatment only normalized the levels of adenosine monophosphate and glycerol. CONCLUSION: Our findings suggest that moxibustion or acupuncture at Zusanli (ST 36) and Zhongwan (CV 12) can significantly improve the condition of CAG in rats. These treatments exert their effects on CAG through different mechanisms. (C) 2020 JTCM. All rights reserved.
