作者机构:
[Wu, Yaohui; Li, Jiaxin; Hu, Yuda; Wang, Yonghong; Zhou, Xuan] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Peoples R China.;[Ning, Ge; Ning, G] Hunan Univ Chinese Med, Int Educ Inst, Changsha 410208, Peoples R China.
通讯机构:
[Wang, YH ] C;[Ning, G ] H;Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Peoples R China.;Hunan Univ Chinese Med, Int Educ Inst, Changsha 410208, Peoples R China.
摘要:
Kanamycin is used widely in livestock farming due to its antimicrobial properties and low cost, but has led to antibiotic residues in food, which can damage human health. Therefore, there is an urgent need for convenient technology that can be used to detect kanamycin rapidly. We found that Co(3)O(4) nanoparticles (NPs) possessed peroxidase-like activity that catalyzed the oxidation of 3,3',5,5'-tetramethylbenzidine to change color. Interestingly, a target-specific aptamer could regulate the catalytic activity of Co(3)O(4) NPs and inhibit this effect through aptamer-target binding. On the basis of a colorimetric assay combined with an aptamer-regulatory mechanism, the linear range for quantitative detection of kanamycin was 0.1-30 μM, the minimum limit of detection was 44.2 nM, and the total time needed for detection was 55 min. Moreover, this "aptasensor" displayed excellent selectivity and could be applied to detect KAN in milk samples. Our sensor might have promising applications for kanamycin detection in animal husbandry and agricultural products.
摘要:
Traditional methods for detecting antibiotic and mycotoxin residues rely on large-scale instruments, which are expensive and require complex sample pretreatment processes and professional operators. Although aptamer-based electrochemical sensors have the advantages of simplicity, speed, low cost, and high sensitivity, most aptamer-based sensors lack a signal amplification strategy due to their direct use of aptamers as probes, resulting in insufficient sensitivity. To solve the sensitivity problem in the electrochemical detection process, a novel electrochemical sensing strategy was established for ultrasensitive zearalenone (ZEN) detection on the basis of exonuclease I (Exo I) and branched hybridization chain reaction (bHCR) to amplify the signal. The amplification strategy showed excellent analytical performance towards ZEN with a low detection limit at 3.1×10(-12) mol/L and a wide linear range from 10(-11) to 10(-6) mol/L. Importantly, the assay was utilized in the corn powder samples with satisfactory results, holding promising applications in food safety detection and environmental monitoring.
期刊:
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy,2023年286:121953 ISSN:1386-1425
通讯作者:
Yonghong Wang<&wdkj&>Ge Ning
作者机构:
[Liu, Yan; Guan, Baibing; Wu, Yaohui; Xu, Ziqi; Wang, Yonghong] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Peoples R China.;[Liu, Yan; Guan, Baibing; Wu, Yaohui; Xu, Ziqi; Wang, Yonghong] Cent South Univ Forestry & Technol, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Peoples R China.;[Ning, Ge] Hunan Univ Chinese Med, Int Educ Inst, Changsha 410208, Peoples R China.;[Liu, Yan] South China Univ Technol, Sch Biol & Biol Engn, Guangzhou 510006, Peoples R China.
通讯机构:
[Yonghong Wang] H;[Ge Ning] I;Hunan Provincial Key Laboratory for Forestry Biotechnology & International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, 410004 Changsha, China<&wdkj&>International Education Institute, Hunan University of Chinese Medicine, 410208 Changsha, China
摘要:
Kanamycin was a group of essential antibiotics generally served in treating infections of animals which leached into the environment residual in food, causing health concerns. Thus, selective and sensitive monitoring of kanamycin was significant for food safety. In this work, split aptamers were used as templates to prepare fluorescent Cu/Ag NCs for detection of kanamycin. According to the impressive affinity of the aptamer to kanamycin, two different detection modes were designed using kanamycin aptamer as a recognition molecule, in which one was to combine split aptamer Apt-1 with Apt-2 to form an entangled DNA as a Cu/Ag NCs template, the other was to associate the normal aptamer after encirclement to form Cu/Ag NCs templates. After the addition of kanamycin, the fluorescence signals of the Cu/Ag NCs synthesized in the two modes were both enhanced, but the approach with split aptamer exhibited a superior observable sensitivity than that of the normal type. The detection range showed a well linear relationship between 80nM and 10μM when the emission wavelength was 560nm, and the detection limit was 13.3nM. In addition, when streptomycin, oxytetracycline, chloramphenicol and chlortetracycline were involved in the selective interference experiment under the same conditions, the fluorescence intensity of the system performed no significant changes. The results demonstrated that this method possessed favorable specificity and selectivity for the assay of kanamycin, proficiently achieving efficient, rapid and sensitive evaluation of kanamycin in the milk samples.
期刊:
Journal of Fluorescence,2022年32(5):1949-1957 ISSN:1053-0509
通讯作者:
Yonghong Wang
作者机构:
[Li, Hong; Xie, Chuchu; Wu, Yaohui; Yao, Liu; Li, Xin; Liao, Zhibin; Wang, Yonghong] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.;[Ning, Ge] Hunan Univ Chinese Med, Int Educ Inst, Changsha 410208, Hunan, Peoples R China.
通讯机构:
[Yonghong Wang] H;Hunan Province Key Laboratory for Forestry Biotechnology, College of Life Science and technology, Central South University of Forestry and Technology, Hunan Province, Changsha, China
作者机构:
[潘江] Hunan University of CM, Changsha 410007, China;[章薇; 娄必丹; 潘江] Department of Acupuncture and Moxibustion;[林碧莲] College of International Education, Hunan University of CM;[陈明] Department of Stomatology, the First Affiliated Hospital of Hunan University of CM, Changsha 410007