隔药饼灸激活Nrf2/ARE/HO-1信号通路修复阿司匹林诱导的胃黏膜损伤的机制研究
作者:
易展;贲定严;徐寅;王佳怡
期刊:
针刺研究 ,2023年48(8):773-781 ISSN:1000-0607
作者机构:
[易展; 贲定严] Xiangxing College, Hunan University of Chinese Medicine, Changsha 410208, China;[王佳怡; 徐寅] Department of Internal Medicine, the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410000
关键词:
胃黏膜损伤;隔药饼灸;核因子E2相关因子2/抗氧化反应元件/血红素加氧酶-1信号通路
摘要:
目的:探讨隔药饼灸是否通过激活核因子E2相关因子2(Nrf2)/抗氧化反应元件(ARE)/血红素加氧酶-1(HO-1)信号通路修复阿司匹林诱导的胃黏膜损伤。方法:SD大鼠随机分为空白对照组、模型组、隔药饼灸穴位组、空白+...展开更多 目的:探讨隔药饼灸是否通过激活核因子E2相关因子2(Nrf2)/抗氧化反应元件(ARE)/血红素加氧酶-1(HO-1)信号通路修复阿司匹林诱导的胃黏膜损伤。方法:SD大鼠随机分为空白对照组、模型组、隔药饼灸穴位组、空白+抑制剂组、模型+抑制剂组、隔药饼灸+抑制剂组,每组20只。阿司匹林(150 mg/kg)灌胃诱导复制胃黏膜损伤模型。隔药饼灸穴位组和隔药饼灸+抑制剂组施隔药饼灸“足三里”“中脘”“脾俞”“胃俞”,每次30 min,连续治疗8 d。3个抑制剂组的大鼠腹腔内注射HO-1抑制剂锌原卟啉5 mg/kg。观察各组大鼠外观行为变化并进行评分;观察胃黏膜损伤程度并对胃黏膜损伤指数进行评定;HE染色观察胃黏膜组织形态学变化;ELISA法检测胃黏膜组织、血清中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。荧光定量PCR法和Western blot法检测胃黏膜组织中Nrf2/ARE/HO-1信号通路相关因子Keap1、Nrf2、HO-1、过氧化氢酶(CAT)、谷胱甘肽S-转移酶(GST)、NAD(P)H醌脱氢酶1(NQO1) mRNA和蛋白表达水平。结果:与空白对照组比较,模型组大鼠的行为状态、情绪反应、皮肤毛发、饮食状态和大便状态评分升高(P<0.01),胃黏膜炎性反应明显,胃黏膜损伤指数升高(P<0.01),胃黏膜组织和血清中SOD活性降低(P<0.01),MDA含量升高(P<0.01),胃黏膜组织中Keap1 mRNA和蛋白表达上调(P<0.01),Nrf2、HO-1、CAT、GST、NQO1 mRNA和蛋白表达下调(P<0.01)。与模型组比较,隔药饼灸穴位组大鼠的情绪反应、皮肤毛发和大便状态评分降低(P<0.01,P<0.05),胃黏膜损伤指数降低(P<0.01),胃黏膜炎性反应减轻,胃黏膜组织和血清中SOD活性升高(P<0.01),MDA含量降低(P<0.01),Keap1 mRNA和蛋白表达下调(P<0.01),Nrf2、HO-1、CAT、GST、NQO1 mRNA和蛋白表达上调(P<0.05,P<0.01)。隔药饼灸+抑制剂组大鼠使用HO-1抑制剂后,与隔药饼灸穴位组比较,皮肤毛发评分升高(P<0.01),胃黏膜损伤指数升高(P<0.05),胃黏膜炎性反应加重,胃黏膜组织和血清中SOD活性降低(P<0.01),MDA含量升高(P<0.01),Keap1 mRNA和蛋白表达上调(P<0.05),HO-1、CAT、GST、NQO1 mRNA和蛋白表达下调(P<0.05,P<0.01),Nrf2蛋白表达下调(P<0.05)。结论:隔药饼灸可通过激活Nrf2/ARE/HO-1信号通路,降低阿司匹林诱导的胃黏膜细胞的氧化应激,从而修复胃黏膜损伤。收起
语种:
中文
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Danger of injudicious use of tui-na therapy in ankylosing spondylitis
作者:
Zou, Gen;Wang, Guyi;Li, Jinxiu;Wu, Guobao;Huang, Jia* ;...
期刊:
European Spine Journal ,2017年26(Suppl 1):178-180 ISSN:0940-6719
通讯作者:
Huang, Jia;Huang, Siyang
作者机构:
[Zou, Gen] Zhejiang Univ, Womens Hosp, Sch Med, Dept Gynaecol, Hangzhou, Zhejiang, Peoples R China.;[Wang, Guyi; Wu, Guobao; Li, Jinxiu; Zou, Gen; Huang, Jia] Cent S Univ, Xiangya Hosp 2, Dept Intens Care Unit, Renmin Rd 139, Changsha, Hunan, Peoples R China.;[Huang, Siyang] Hunan Univ Chinese Med, XiangXing Coll, Changsha, Hunan, Peoples R China.
通讯机构:
[Huang, Jia] C;[Huang, Siyang] H;Cent S Univ, Xiangya Hosp 2, Dept Intens Care Unit, Renmin Rd 139, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, XiangXing Coll, Changsha, Hunan, Peoples R China.
关键词:
Ankylosing spondylitis;Cervical dislocation;Tui-na therapy
摘要:
Tui-na is a very important component of Chinese medicine. It is a well-respected treatment modality known to be helpful and safe for a wide range of conditions. It can be considered as a certain kind of massage which is performed at acupoints, meridians, and collaterals by pushing, finger twisting, grasping thumb waving pressing, patting, palm twisting, and other manipulation techniques. At present, it is extensively used for relieving pain and stiffness associated with ankylosing spondylitis in China, even though there is a lack of evidence to support its validation and feasibility. The patient in this case was treated by tui-na massage at acute flare-up of ankylosing spondylitis and ended up with catastrophic results. © 2017 The Author(s)
语种:
英文
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隔药饼灸治疗老年骨质疏松症临床研究
作者:
曾宇晴;贲定严;易展
( 易展 ) ;陆建伟;钟甫华;...
期刊:
中国针灸 ,2017年37(5):473-476 ISSN:0255-2930
作者机构:
[易展; 陆建伟; 钟甫华; 江彬锋] Department of Orthopedics and Traumatology, Zhejiang Tongde Hospital, Hangzhou 310012, China;[贲定严] College of Acupuncture-Moxibustion and Tuina, Hunan University of CM;[Yi, Zhan] College of Xiangxing, Hunan University of CM, Changsha 410007
关键词:
老年骨质疏松症;隔药饼灸;骨密度(BMD);Ⅰ型前胶原氨基端前肽(PINP);β-Ⅰ型胶原羧基端肽(β-CTX);随机对照试验
摘要:
目的:探讨隔药饼灸治疗老年骨质疏松症的临床疗效及其部分作用机制。方法:将60例老年骨质疏松症患者按随机数字表法随机分为观察组和对照组,每组30例。两组均予西药基础治疗,穴取①大椎、大杼、肝俞;②中脘、膻中、足三里;③脾俞、肾俞、命门;④神阙、关元。每次取1组穴位,4组穴位轮流选用。观察组予隔药饼灸,药物组成:补骨脂30 g、熟地黄30 g、白术30 g、党参30 g、淫羊藿20 g、仙茅20 g、丁香5 g、肉桂5 g。对照组予隔面饼灸。两组均每穴施灸5壮,隔日治疗1次,共治疗6个月。观察两组治疗前后的症状评分、腰椎及髋部骨密度(BMD)、血清Ⅰ型前胶原氨基端前肽(PINP)和β-Ⅰ型胶原羧基端肽(β-CTX)。结果:两组治疗后症状评分、血清β-CTX均低于治疗前,腰椎及髋部BMD、血清PINP均高于治疗前(均P<0.05);治疗后观察组症状评分、血清β-CTX均低于对照组,腰椎及髋部BMD、血清PINP均高于对照组(均P< 0.05)。结论:隔药饼灸治疗老年骨质疏松症疗效显著,优于隔面饼灸。
语种:
中文
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