期刊：
Journal of Ethnopharmacology,2024年320:117417 ISSN：0378-8741
通讯作者：
Jin, J
作者机构：
[Zhang, Shuihan; Jin, Jian; Liu, Tingting; Jin, J; Shen, Bingbing; Zeng, Hongliang; Qin, You; Xie, Zhenni] Hunan Acad Chinese Med, Inst Chinese Med Resources, Changsha 410013, Hunan, Peoples R China.;[Zhang, Shuihan; He, Dan; Zeng, Hongliang; Xie, Zhenni] Hunan Univ Chinese Med, Grad Sch, Changsha 410036, Hunan, Peoples R China.;[Luo, Ji] Hunan Acad Chinese Med, Affiliated Hosp, Changsha 410013, Hunan, Peoples R China.
通讯机构：
[Jin, J ] H;Hunan Acad Chinese Med, Inst Chinese Med Resources, Changsha 410013, Hunan, Peoples R China.
关键词：
Anti-proliferation;MAPK3;PIK3R3;Poria cocos triterpenoids component fraction;Stomach cancer
摘要：
ETHNOPHARMACOLOGICAL RELEVANCE: Poria cocos F.A. Wolf is an edible fungus with forming sclerotia, which has the effects of promoting diuresis, exuding dampness, invigorating the spleen, and regulating the stomach. P. cocos has a high application in the clinic of traditional Chinese medicine, and some studies have indicated that P. cocos has a good effect on tumor diseases. According to ancient records and modern studies, P. cocos wine offers beneficial effects in terms of strengthening tendons and bones and anti-tumor effects. AIM OF THE STUDY: To understand the substance composition of P. cocos ethanol-soluble extract (PESE) and then further study the effect and potential mechanism of PESE components on gastric cancer. MATERIALS AND METHODS: In vitro and in vivo experiments were performed to detect the cell activity and apoptotic condition. Differential expression analysis and pathway enrichment were performed based on transcriptomics and were verified by real-time polymerase chain reaction and western blotting. The mice of the stomach cancer tumor model were randomly categorized into three groups. The weight and tumor volume of the mice were measured, and the pathological characteristics of tumor tissue and immunohistochemical changes were determined. Then, the main active components of PESE were detected by MKN45cell fishing. RESULTS: In vitro experiments showed that PESE inhibited the proliferation of MKN45cells, but it did not induce apoptosis. Based on the transcriptome and western blotting results, the inhibition of MKN45 proliferation by PESE may be influenced by mitogen-activated protein kinase (MAPK) and phosphoinositide-3-kinase-protein kinase B (PI3K-Akt) signaling pathways. In vivo experiments showed that PESE inhibited tumor growth in mice and caused partial necrosis of tumor cells but had no toxic effect on mice. Cell fishing identified nine triterpenoids of P. cocos as the major active components of PESE. CONCLUSIONS: The results indicated that PESE has a significant inhibitory effect on stomach cancer, and its mechanism probably commonly affects the MAPK and PI3K-Akt signaling pathways, which could be due to the triterpenoid components.
期刊：
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2023年19(1):109-116 ISSN：1550-7033
作者机构：
[Jiang, Xueyu; Yan, Sen; Liu, Zhili; Fu, Mandi] Yueyang Tradit Chinese Med Hosp, Dept Massage, Yueyang 414000, Peoples R China.;[Zhu, Wei] Hunan Inst Tradit Chinese Med, Dept Acupuncture & Moxibust, Affiliated Hosp, Changsha 410000, Peoples R China.;[Ren, Xiang] Hunan Univ Tradit Chinese Med, Grad Sch, Changsha 410208, Peoples R China.;[Hu, Lin] Yueyang Tradit Chinese Med Hosp, Dept Anesthesiol, Yueyang 414000, Peoples R China.;[Chang, Xiaorong; Zhang, Guoshan; Liu, Qiong] Hunan Univ Tradit Chinese Med, Coll Acupuncture & Massage, Changsha 410208, Peoples R China.
关键词：
Cervical Spondylotic Radiculopathy;Neuralgia of Cervical Spondylotic;Electro-Acupuncture;Neuron-Gliocyte-Chemokine;Mechanism
摘要：
Background: Cervical spondylotic radiculopathy (CSR) is the most common type of cervical spondylosis, which mainly presents as radioactive neuralgia. Neuralgia is closely related to gliocyte in the central nervous system. Therefore, to investigate the therapeutic effect and mechanism of LAA (astrocyte inhibitor) electro-acupuncture on neuralgia in rats with cervical spondylotic radiculopathyIP: based203.8.109on the20 On: Wed, 03 neuron-gliocyte-chemokine May 2023 10:21:59 signaling pathway. Material & Methods: The models of rats with neuralgia in cervical spondylotic rdiculopathy were etablihed and the intervention of electro-Copy ight: Amer can Scientific Publishers acupuncture and LAA were carried out. All rats were randomldivided into 4 groups (12 rats in each group). Control Delivered by ngenta group, Model group, Sham operation group and Electro-acupuncture group. Results: The rats were bound to the plate in a prone position and treated with electro-acupuncture at Jiaji point, with dense wave frequency of 50 Hz and wave repacking frequency of 10 Hz, once a day, 20 min each time, for 14 consecutive days. The results found that the content of calcitonin gene-related peptides (CGRP), adenylate cyclase (AC), substances P in C6-C7 primary sensory neuron terminal of the spinal cord segment were significantly decreased in electro-acupuncture group compared with model group. Relative expression of RNA levels of protein kinase (PKC), Ca2+ channel protein (VGCC, CCL2 and CXC3L1) in electro-acupuncture group were significantly decreased compared with model group. Westernblot detected that the expression of IL-1/3, IL-6, IL-18, TNF-a, NMDA and AMPA proteins in LAA +electro+ acupuncture group were obvioulsy decreased. It indicates that neuralgia is closely related to gliocyte in the central nervous system, and also proves that LAA +electro acupuncture can effectively relieve neuralgia caused by cervical spondylosis.
期刊：
Scandinavian Journal of Rheumatology,2023年52(2):200-207 ISSN：0300-9742
通讯作者：
Wu, B.;Li, C.
作者机构：
[Li, C.; Yu, X.; Wu, B.; Wu, B; Li, C] Chongqing Hosp Tradit Chinese Med, Basic Res Dept Tradit Chinese Med & Pharm, Chongqing 400021, Peoples R China.;[Li, C.; Wu, B.; Wang, J.; Zhu, F.] Chongqing Hosp Tradit Chinese Med, Dept Rheumatol, Chongqing 400021, Peoples R China.;[Yu, X.] Hunan Univ Tradit Chinese Med, Grad Sch, Changsha, Peoples R China.
通讯机构：
[Wu, B; Li, C] C;Chongqing Hosp Tradit Chinese Med, Basic Res Dept Tradit Chinese Med & Pharm, Chongqing 400021, Peoples R China.
摘要：
OBJECTIVE: The aim of this study was to explore the significance of serum CCL28 in Sjögren's syndrome (SS) diagnosis and evaluation. METHOD: The expression of CCL28 mRNA in salivary glands of SS patients from the GEO database was analysed. Serum levels of CCL28 of SS patients, rheumatoid arthritis (RA) patients, systemic lupus erythematosus (SLE) patients, and healthy controls (HCs) were measured by enzyme-linked immunosorbent assay. The serum immunoglobulin A (IgA) levels and the focus score of labial salivary gland (LSG) in patients with SS were also measured, and the correlation between serum IgA levels and serum CCL28 was explored. In addition, the level of serum CCL28 was compared between two subsets of SS patients who were classified by clinical symptoms and laboratory tests. RESULTS: SS patients displayed decreased expression of CCL28 mRNA in salivary glands, accompanying more severe pathological injury. Serum levels of CCL28 in both primary and secondary SS patients were significantly lower than those in the HC group, whereas no significant differences were observed between RA patients or SLE patients and HCs. Compared with RA and SLE patients alone, serum levels of CCL28 were dramatically lower in patients with SS secondary to RA or SLE. No remarkable correlation between serum IgA and CCL28 levels was observed, while the focus score of LSG negatively correlated with serum CCL28 levels. Serum levels of CCL28 were lower in SS patients who had dental caries and thrombocytopenia. CONCLUSION: Serum CCL28 is a useful biomarker in the diagnosis and evaluation of SS.
作者机构：
[林旭鑫] Graduate School, Hunan University of Chinese Medicine, Changsha Hunan, 410208, P. R. China;[尚利杰; 付豪; 林旭鑫; 周鹭凡; 常青; 赵刚; 王怡帆] First Department of Minimally Invasive Spine, Luoyang Orthopedic-Traumatological Hospital of Henan Province (Henan Provincial Orthopedic Hospital), Luoyang Henan, 471000, P. R. China;[沈素红; 付卓] Department of Functional Examination, Luoyang Orthopedic-Traumatological Hospital of Henan Province (Henan Provincial Orthopedic Hospital), Luoyang Henan, 471000, P. R. China
