Phenotypic characterization of type II collagen-induced arthritis in Wistar rats
作者:
Song, Hou-Pan
(宋厚盼);Li, Xin
(李鑫);Yu, Rong
(喻嵘);Zeng, Guang;Yuan, Zhen-Yi
(袁振仪);...
期刊:
Experimental and Therapeutic Medicine,2015年10(4):1483-1488 ISSN:1792-0981
作者机构:
[Song, Hou-Pan; Li, Xin; Zeng, Guang; Huang, Hui-Yong; Cai, Xiong] Hunan Univ Chinese Med, Hunan Prov Key Lab Diagnost Chinese Med, Changsha 410007, Hunan, Peoples R China.;[Yu, Rong; Yuan, Zhen-Yi; Cai, Xiong] Hunan Univ Chinese Med, Hunan Dept, Educ Key Lab Translat Chinese Med, Changsha 410007, Hunan, Peoples R China.;[Wang, Wei] Hunan Univ Chinese Med, Sch Pharmaceut Sci, Changsha 410007, Hunan, Peoples R China.;[Cai, Xiong] Hunan Univ Chinese Med, Hunan Prov Key Lab Diagnost Chinese Med, 113C Shaoshan Rd, Changsha 410007, Hunan, Peoples R China.
通讯机构:
[Cai, Xiong] H;Hunan Univ Chinese Med, Hunan Prov Key Lab Diagnost Chinese Med, 113C Shaoshan Rd, Changsha 410007, Hunan, Peoples R China.
关键词:
Wistar rats;bovine type II collagen;collagen-induced arthritis;incomplete Freund's adjuvant
摘要:
The aim of the present study was to determine a more specific, efficient and simple method for the induction of collagen-induced arthritis (CIA) in rats. Different strains of rats were injected at the base of the tail with bovine type II collagen (CII) emulsified in incomplete Freund's adjuvant (IFA). The onset and severity of arthritis were evaluated by clinical assessment. The established CIA model was analyzed using a comprehensive examination of clinical, hematological, histological and radiological parameters. The results demonstrated that Wistar rats were the most susceptible strain to CIA followed by Wistar Furth rats, with Sprague Dawley rats being the least susceptible. Following primary and booster immunization, female Wistar rats developed severe arthritis, with an incidence of >83% and low variability in clinical signs. The development of arthritis was accompanied by a significantly elevated erythrocyte sedimentation rate compared with that in the control rats. The radiographic examination revealed bone matrix resorption, considerable soft tissue swelling, periosteal new bone formation and bone erosion in the arthritic joints of the CIA rats. Histopathologically, the synovial joints of CIA rats were characterized by synovial hyperplasia, pannus formation, marked cellular infiltration, bone and cartilage erosion and narrowing of the joint space. The administration of an intradermal injection of only 200 μg bovine CII emulsified in IFA at the base of the tail therefore leads to the successful development of a CIA rat model. This well-characterized CIA rat model could be specifically used to study the pathophysiology of human rheumatoid arthritis as well as to test and develop anti-arthritic agents for humans. © 2015, Spandidos Publications. All rights reserved.
语种:
英文
展开