EXPERIMENTAL AND THERAPEUTIC MEDICINE,2015年10(4):1483-1488 ISSN：1792-0981
[Song, Hou-Pan; Li, Xin; Zeng, Guang; Huang, Hui-Yong; Cai, Xiong] Hunan Univ Chinese Med, Hunan Prov Key Lab Diagnost Chinese Med, Changsha 410007, Hunan, Peoples R China.;[Yu, Rong; Yuan, Zhen-Yi; Cai, Xiong] Hunan Univ Chinese Med, Hunan Dept, Educ Key Lab Translat Chinese Med, Changsha 410007, Hunan, Peoples R China.;[Wang, Wei] Hunan Univ Chinese Med, Sch Pharmaceut Sci, Changsha 410007, Hunan, Peoples R China.
[Cai, X] Hunan Univ Chinese Med, Hunan Prov Key Lab Diagnost Chinese Med, 113C Shaoshan Rd, Changsha 410007, Hunan, Peoples R China.
collagen-induced arthritis;bovine type II collagen;incomplete Freund's adjuvant;Wistar rats
The aim of the present study was to determine a more specific, efficient and simple method for the induction of collagen-induced arthritis (CIA) in rats. Different strains of rats were injected at the base of the tail with bovine type II collagen (CII) emulsified in incomplete Freund's adjuvant (IFA). The onset and severity of arthritis were evaluated by clinical assessment. The established CIA model was analyzed using a comprehensive examination of clinical, hematological, histological and radiological parameters. The results demonstrated that Wistar rats were the most susceptible strain to CIA followed by Wistar Furth rats, with Sprague Dawley rats being the least susceptible. Following primary and booster immunization, female Wistar rats developed severe arthritis, with an incidence of >83% and low variability in clinical signs. The development of arthritis was accompanied by a significantly elevated erythrocyte sedimentation rate compared with that in the control rats. The radiographic examination revealed bone matrix resorption, considerable soft tissue swelling, periosteal new bone formation and bone erosion in the arthritic joints of the CIA rats. Histopathologically, the synovial joints of CIA rats were characterized by synovial hyperplasia, pannus formation, marked cellular infiltration, bone and cartilage erosion and narrowing of the joint space. The administration of an intradermal injection of only 200 jig bovine CII emulsified in IFA at the base of the tail therefore leads to the successful development of a CIA rat model. This well-characterized CIA rat model could be specifically used to study the pathophysiology of human rheumatoid arthritis as well as to test and develop anti-arthritic agents for humans.
Phytomedicine : international journal of phytotherapy and phytopharmacology,2017年28:27-35 ISSN：0944-7113
Cai, Xiong;Zhou, Chi
[Li, Xin; Huang, Hui-Yong; Li, Ru-Yi; Song, Hou-Pan; Yu, Rong; Cai, Xiong] Hunan Provincial Key Laboratory of Diagnostic and Therapeutic Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;[Zhou, Sai-Nan] The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;[Hou, Xue-Qin] Institute of Pharmacology, Taishan Medical College, Taian, Shandong 271000, China;[Zhou, Chi] The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, China. Electronic address: email@example.com;[Cai, Xiong] School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China. Electronic address: firstname.lastname@example.org
[Cai, Xiong] School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China. Electronic address:;[Zhou, Chi] The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, China. Electronic address:
Atractylenolide I;Intestinal epithelial repair;Cell migration and proliferation;Polyamine;Cytosolic free Ca2+
Background : An impairment of the integrity of the mucosal epithelial barrier can be observed in the course of various gastrointestinal diseases. The migration and proliferation of the intestinal epithelial (IEC-6) cells are essential repair modalities to the healing of mucosal ulcers and wounds. Atractylenolide I (AT-I), one of the major bioactive components in the rhizome of Atractylodes macrocephala Koidz. (AMR), possesses multiple pharmacological activities. This study was designed to investigate the therapeutic effects and the underlying molecular mechanisms of AT-I on gastrointestinal mucosal injury. Methods: Scratch method with a gel-loading microtip was used to detect IEC-6 cell migration. The real-time cell analyzer (RTCA) system was adopted to evaluate IEC-6 cell proliferation. Intracellular polyamines content was determined using high performance liquid chromatography (HPLC). Flow cytometry was used to measure cytosolic free Ca2+ concentration ([Ca2+](c)). mRNA and protein expression of TRPC1 and PLC-gamma(1) were determined by real-time PCR and Western blotting assay respectively. Results: Treatment of IEC-6 cells with AT-I promoted cell migration and proliferation, increased polyamines content, raised cytosolic free Ca2+ concentration ([Ca2+] c), and enhanced TRPC1 and PLC-gamma(1) mRNA and protein expression. Depletion of cellular polyamines by DL-a-difluoromethylornithine (DFMO, an inhibitor of polyamine synthesis) suppressed cell migration and proliferation, decreased polyamines content, and reduced [Ca2+] c, which was paralleled by a decrease in TRPC1 and PLC-gamma(1) mRNA and protein expression in IEC-6 cells. AT-I reversed the effects of DFMO on polyamines content, [Ca-2+] c, TRPC1 and PLC-gamma(1) mRNA and protein expression, and restored IEC-6 cell migration and proliferation to near normal levels. Conclusion: Our data demonstrate that AT-I stimulates intestinal epithelial cell migration and proliferation via the polyamine-mediated Ca2+ signaling pathway. Therefore, AT-I may have the potential to be further developed as a promising therapeutic agent to treat diseases associated with gastrointestinal mucosal injury, such as inflammatory bowel disease and peptic ulcer. (C) 2017 Elsevier GmbH. All rights reserved.