作者：
Jun Liao（廖君）;Xing Xia;Guo-zuo Wang;Yong-mei（石咏梅）;Shi Jin-wen Ge
作者机构：
[Yong-mei; Jun Liao; Xing Xia; Guo-zuo Wang; Jin-wen Ge] Department of Anatomy,Hunan University of Chinese Medicine;[Yong-mei; Jun Liao; Xing Xia; Guo-zuo Wang; Jin-wen Ge] Campus Network Center,Hunan University of Chinese Medicine;[Yong-mei; Jun Liao; Xing Xia; Guo-zuo Wang; Jin-wen Ge] College of Integrated Medicine,Hunan University of Chinese Medicine;[Yong-mei; Jun Liao; Xing Xia; Guo-zuo Wang; Jin-wen Ge] Hunan Province Key Laboratory of Integrated Medicine for Cardi-Cerebrovascular Disease,Hunan University of Chinese Medicine
摘要：
Objective: To observe the expression ofFerroportin (Fpn) at different time points in rats after focal cerebral ischemia treated with or without Chinese medicine NTE (extract of HuangQi, Chuan Xiong, Di Long, Jiang Can)_Methods: The experiment is carried out by two steps: Firstly , SD rats were randomly divided into 2 h, 6 h, 12 h, 24 h, 72 h groups after operation of Middle cerebral artery occlusion (MCAO), mRNA and protein level of Fpn were detected by immunohistochemistry and RT-PCR at above time points; Secondly, the rats were randomly allotted five groups as following: sham operation group, model group, low dose group of NTE (3 g/kg), medial dose group of NTE (9_g/kg), large dose group of NTE (27 g/kg).After three days of corresponding therapy by intragastric administration once a day, the regional cerebral ischemia model was reproduced by middle cerebral artery occlusion (MCAO)with suture method.Three days later, the rats treated by previous method.The third day, Neurological behavior of rats were detected by neurobehavioral testing.Fpn in Hippocampal C2 region was measured by immunohistochemical method and mRNA of Fpn was detected by RT-PCR.Results: Expression of Fpn in hippocamp C2 reaches the highest point at 12 h after operation (P<0.05); compared with model group, the large dose group of NTE (27 g/kg) displays a lower Neurological behavior score (P<0.05); and large dose group ofNTE showes higher lever expression of Fpn both at mRNA and protein level compared with sham operation group and model group (P<0.05).Conclusion: Disregulation of intracellular iron balance perhaps is the new mechanism of cerebral ischemia injury, the NTE can protect the neuron in Hippocampal C2 area by adjusting the expression of Fpn to balance the iron after cerebral ischemia.
