作者机构：
[Qiong LIU; Wei ZHU; Mailan LIU; Xia LIU; Jianan CAO; Shuning HU; Xiaorong CHANG] College of Acupuncture & Moxibustion & Tui-na, Hunan University of Chinese Medicine, Changsha 410007, China (湖南中医药大学针灸推拿学院, 长沙 410007, 中国);[Xianghong JING] Laboratory of Meridians and Collaterals, Institute of Acupuncture & Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China (中国中医科学院针灸研究所经络研究室, 北京 100700, 中国)
通讯机构：
[Xiaorong CHANG] C;[Xianghong JING] L;Laboratory of Meridians and Collaterals, Institute of Acupuncture & Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China (中国中医科学院针灸研究所经络研究室, 北京 100700, 中国)<&wdkj&>College of Acupuncture & Moxibustion & Tui-na, Hunan University of Chinese Medicine, Changsha 410007, China (湖南中医药大学针灸推拿学院, 长沙 410007, 中国)
摘要：
Objective: To observe the influence of acupuncture stimulus and thermal stimulus on the expression of mast cells(MCs) and 5-hydroxytryptamine(5-HT) in local acupoint area of Guanyuan (关元 CV 4) and serum 5-HT in rats. Methods: Thirty-nine male C57BL/6 rats were randomly divided into blank control group, acupuncture stimulus group and thermal stimulus group, with 13 rats in each group. The rats were stimulated by manual acupuncture at CV 4 for 5 min in acupuncture stimulus group, while those in thermal stimulus group were stimulated by adopting a thermal moxibustion apparatus on CV 4 for 30 min. The expression of MC and 5-HT in the skin in the acupoint area of CV 4 before and after acupuncture stimulus and thermal stimulus was observed and analyzed via adopting toluidine blue staining method and immunofluorescence histochemical method(5 rats were selected from each group), and the 5-HT content in serum before and after stimulus was determined through enzyme-linked immunosorbent assay(ELISA)(8 rats were selected from each group). The influence of acupuncture stimulus and thermal stimulus on the contents of MC and 5-HT in the skin and serum 5-HT in rats was analyzed and compared. Results:① After acupuncture stimulus or thermal stimulus on CV 4 of C57BL/6 rats, the number of MC in the acupoint area significantly increased when compared with that in blank group(the acupuncture stimulus group 12.40 ±2.07 vs. the blank group 3.00 ± 5.96; thermal stimulus group 26.20 ± 10.85 vs. the blank group 12.40± 2.07, both P< 0.05), and MC aggregation and degranulation were observed (the acupuncture stimulus group 17.80 ± 4.55 vs. the blank group 8.00 ± 3.16; the thermal stimulus group 24.00 ± 9.05 vs. the blank group 8.00 ± 3.16, P<0.05, P<0.01).② After acupuncture stimulus or thermal stimulus, 5-HT was released by MCs in the acupoint area, which aggregated around the blood vessels, and the number of 5-HT in the acupoint area significantly increased when compared with that in blank group (the blank group 3.00 ±1.28 vs. the acupuncture stimulus group 10.02 ± 3.21; the blank group 3.00 ± 1.28 vs. the thermal stimulus group 14.00 ± 3.94, both P < 0.01 ).③ Compared with blank group, both acupuncture stimulus and thermal stimulus could reduce the 5-HT content in serum(the blank group 0.72 ± 0.2372 vs. acupuncture stimulus group 0.43 ±0.21; the blank group 0.72 ± 0.24 vs. thermal stimulus group 0.32 ± 0.18, both P< 0.01), and the effect in thermal stimulus group was slightly superior to that in acupuncture stimulus group(P<0.05). Conclusion: Both acupuncture stimulus and thermal stimulus can cause the aggregation and degranulation of MCs and high expression of 5-HT in the acupoint area. The effect of thermal stimulus was superior to that of acupuncture stimulus in degranulation.
