作者机构:
Department of Traditional Chinese Medicine of Hunan Provincial People's Hospital, Changsha, 410005;School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410007;[封迎帅; 刘百祥; 李俊; 阳大为] Department of Traditional Chinese Medicine of Hunan Provincial People's Hospital, Changsha, 410005;[林亚平] School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410007
通讯机构:
[Ya-ping Lin] S;School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, China
摘要:
Objective: To observe the effect of electroacupuncture (EA) at Huantiao (GB 30) and Weizhong (BL 40) on thigmesthesia, gait function, and expression levels of serum immunoglobulin G (IgG) and immunoglobulin M (IgM) in rabbits with lumbar intervertebral disc herniation (LIDH). Methods: Forty healthy New Zealand rabbits were randomly divided into a blank control group, a model group, an EA at acupoint group and an EA at non-acupoint group, with 10 rabbits in each group. The LIDH pathological model of rabbit was established using the self-made LIDH model maker. The thigmesthesia and gait function of rabbits were recorded by Siegal method. The serum IgG and IgM expression levels were detected by enzyme-linked immunosorbent assay. Results: EA at Huantiao (GB 30) and Weizhong (BL 40) could improve the clinical symptoms of thigmesthesia and gait function, and inhibit the expressions of serum IgG and IgM in the LIDH rabbits, which were significantly different compared with those in the model group and EA at non-acupoint group. Conclusion: EA at Huantiao (GB 30) and Weizhong (BL 40) can improve the clinical symptoms of LIDH rabbits, which is associated with inhibition of the serum IgG and IgM expressions and reduction of the immunoinflammatory factor release. This may be one of the mechanisms of EA at Huantiao (GB 30) and Weizhong (BL 40) in the treatment of LIDH.
作者机构:
Graduate School, Hunan University of Chinese Medicine, Changsha, 410208;Key Laboratory of Acupuncture Bioinformation Analysis, School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410208;[张程程; 陈海交; 杨建文; 刘薇薇; 刘丽] Graduate School, Hunan University of Chinese Medicine, Changsha, 410208;[林亚平; 彭艳] Key Laboratory of Acupuncture Bioinformation Analysis, School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410208
通讯机构:
[Yan Peng] K;Key Laboratory of Acupuncture Bioinformation Analysis, School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, China
关键词:
Acupuncture Therapy;Electroacupuncture;Interstitial Cells of Cajal;Diabetes Complications;Gastroparesis;Rats
摘要:
Objective: To observe the effect of electroacupuncture (EA) at Zusanli (ST 36) on the gastrointestinal motility and the ultrastructures of pacemaker cells [the interstitial cells of Cajal (ICC)] in diabetic gastroparesis (DGP) rats and explore the mechanism of EA for DGP. Methods: A total of 50 Sprague-Dawley (SD) rats were randomly divided into group A, group B, group C, group D and group E, with 10 rats in each group. Group A was the blank control; a single intraperitoneal injection of 2% streptozotocin (STZ) was performed in rats of group B, group C, group D and group E, with high glucose and high fat diet for 8 weeks to establish the DGP rat models. Group B was the model group and the rats did not receive any treatment; group C was EA at acupoint group and the rats received EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6); group D was EA at non-acupoint group and the rats received EA at the control points of Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6); group E was metoclopramide group and the rats were treated by intragastric administration of metoclopramide. Blood glucose was detected using ONE TOUCH blood glucose meter; gastric emptying rate and small intestine migration rate were measured using intragastric phenol red; ultrastructures of gastric antrum ICC were detected by transmission electron microscopy. Results: The differences of blood glucose between group B, group C, group D, group E versus that of group A were statistically significant after modeling (P<0.01); after treatment, the differences of blood glucose between group D, group E versus that of group C were statistically significant (P<0.05, P<0.01); the gastric emptying rate of rats in group B was statistically significant different from that in group A (P<0.01); the gastric emptying rate of rats in group C was statistically significant different from that in group B (P<0.01). The migration rates of rats’ small intestines in group B, group C, group D and group E were all statistically significant different from that in group A (P<0.01); the migration rate of rats’ small intestines in group C was statistically significant different from that in group B (P<0.01). The ultrastructure of rat’s ICC in group B showed apoptosis compared with that in group A; rat‘s ICC in group C had complete basement membrane, more cytoplasm mitochondria, Golgi and rough endoplasmic reticulum, showing clear structure, occasional mitochondria swelling and gap junctions with adjacent smooth muscle cells; there were no significant differences between group D, group E versus group B. Conclusion: EA at Zusanli (ST 36) plus other acupoints can regulate the blood glucose and promote gastrointestinal motility in DGP rats, and the mechanism may be related to repairing the damaged ICC structure.
摘要:
Objective: To investigate the effect of acupuncture plus mild hypothermia on neurological function impairment score, cerebral infarct size and apoptosis-related factors in cerebral ischemia reperfusion injury (CIRI) rats. Methods: Sixty healthy male Sprague-Dawley (SD) rats were routinely reared for 1 week. Ten rats were randomly selected as the sham operation group and 10 rats as the blank control group, while the remaining 40 rats were subjected to preparing the middle cerebral artery occlusion (MCAO) model by modified filament occlusion method. The 40 MCAO rats were further randomly divided into a model group, an acupuncture group, a mild hypothermia group and an acupuncture plus mild hypothermia group, with 10 rats in each group. Rats in the sham operation group, the blank control group and the model group did not accept treatment except binding; rats in the acupuncture group received acupuncture treatment; rats in the mild hypothermia group received mild hypothermia treatment; rats in the acupuncture plus mild hypothermia group received acupuncture and mild hypothermia treatment. 72 h after the treatment, neurological function impairment score was performed; the infarct area ratio was determined by 2,3,5-tripheyl tetrazolium chloride (TTC) staining; apoptosis of brain cells was observed by TUNEL method; the expressions of Bcl-2, Bax and Caspase-3 were detected by immunohistochemistry. Results: Compared with the blank control group and the sham operation group, the neurological function impairment score, cerebral infarct area ratio, apoptosis, and the expressions of Bax and Caspase-3 in the model group were significantly increased, while the expression of Bcl-2 was significantly decreased, and there were significant between-group differences (all P<0.05). After the treatment, there were statistically significant differences among the treatment groups in the neurological function impairment score, cerebral infarct area ratio and apoptosis in the ischemic side of rats, as well as the expressions of Bcl-2, Bax and Caspase-3 (all P<0.05), and from the figures, tables and statistical analysis, it was found that a better tendency in the acupuncture plus mild hypothermia group than the acupuncture group or mild hypothermia group. Conclusion: Acupuncture plus mild hypothermia can protect the brain cells by improving neurological function impairment, decreasing cerebral infarct area ratio, reducing the number of apoptotic cells in the ischemic area and regulating the expressions of apoptosis related proteins to inhibit apoptosis.
作者机构:
School of Acupuncture,Moxibustion & Tuina,Hunan University of Chinese Medicine, Changsha, 410208;Postgraduate School of Hunan University of Chinese Medicine, Changsha, 410208;[李文慧; 谭杏; 宋洋] School of Acupuncture,Moxibustion & Tuina,Hunan University of Chinese Medicine, Changsha, 410208;[郁洁; 林亚平] Postgraduate School of Hunan University of Chinese Medicine, Changsha, 410208
通讯机构:
[Ya-ping Lin] P;Postgraduate School of Hunan University of Chinese Medicine, Changsha, China
摘要:
Objective:To investigate the effects of electroacupuncture(EA)at Neiguan(PC 6)and Baihui(GV 20)by observing the changes of CCAAT/enhancer-binding protein(C/EBP)homologous protein(CHOP)and caspase-12 gene expressions in rats after cerebral ischemia-reperfusion injury(IRI),and explore whether the apoptosis pathway of endoplasmic reticulum stress(ERS)is involved in the protective mechanisms of EA.Methods:Sixty rats were randomly assigned to five groups(12 in each group):a normal control group(group A),a sham-operation group(group B),an operation group(group C),an Edaravone group(group D)and an EA group(group E).The cerebral IRI rat model was induced by middle cerebral artery occlusion(MCAO)using intraluminal monofilament.2,3,5-triphenyl tetrazolium chloride(TTC)staining was adopted in the measurement of cerebral infarction volume.Real-time polymerase chain reaction(RT-PCR)was used to determine the mRNA expressions of CHOP and caspase-12.Results:Compared with group A and group B,the volume of cerebral infarction and mRNA expressions of CHOP and caspase-12 in group C,group D and group E were increased,with statistical significances(P<0.05 or P<0.01);compared with group C,the volume of cerebral infarction and mRNA expressions of CHOP and caspase-12 in group D and group E were decreased significantly(P<0.05 or P<0.01);there were no significant differences between group D and group E in comparing the above items(P>0.05).Conclusion:EA at Neiguan(PC 6)and Baihui(GV 20)can effectively suppress the volume of cerebral infarction.Furthermore,the underlying mechanism of EA at Neiguan(PC 6)and Baihui(GV 20)is possibly related to the down-regulation of CHOP and caspase-12 mRNA expressions,so as to decrease cell apoptosis.
作者机构:
[杨建文; 刘丽; 刘薇薇; 陈海交; 张程程] Graduate School, Hunan University of Chinese Medicine, Changsha 410007, China;[林亚平; 彭艳; 岳增辉] Key Acu-moxibustion Laboratory of Biological Information Analysis of Institute of Acupuncture, Moxibustion and Massage, Hunan University of Chinese Medicine, Changsha 410007, China
作者机构:
[杨建文; 刘丽; 刘薇薇; 陈海交; 张程程] Graduate School, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[彭艳; 刘密] Key Lab for Acu-moxibustion Biological Information Analysis of College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[林亚平] Key Lab for Acu-moxibustion Biological Information Analysis of College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410208, China. lyp5381161@126.com
作者机构:
[彭艳; 林亚平; 贺凤娥; 万荃荃; 陈文; 刘琴; 易受乡] Major Laboratory of Meridians and Viscera, Tertiary Laboratory of State Administration of Traditional Chinese Medicine, Institute of Acupuncture, Moxibustion and Massage, Hunan University of Chinese Medicine, Changsha, 410007
通讯机构:
[Ya-ping Lin] M;Major Laboratory of Meridians and Viscera, Tertiary Laboratory of State Administration of Traditional Chinese Medicine, Institute of Acupuncture, Moxibustion and Massage, Hunan University of Chinese Medicine, Changsha, China
摘要:
Objective: To explore the action mechanism of electroacupuncture (EA) in improving the gastric motility of rats with diabetic gastroparesis (DGP). Methods: Forty-eight healthy Sprague-Dawley (SD) rats were randomly assigned into four groups: a normal group (group A), a model group (group B), a group of EA at acupoints (group C), and a group of EA at non-acupoints (group D), 12 rats in each group. The animal model of DGP was established by intraperitoneal injection of streptozotocin (STZ) plus high glucose and fat diet. The blood glucose, urine glucose and gastric emptying rate (GER) were observed. The content of insulin (INS) in serum and ghrelin in gastric antrum tissue were detected by enzyme linked immunoassay (ELISA). The expression of growth hormone secretagogue receptor mRNA (GHSR mRNA) in gastric antrum tissue was detected by real-time fluorescent quantitative polymerase chain reaction (PCR). Results: Compared with group A, blood glucose and urine glucose increased significantly (P<0.01), GER, content of serum insulin, the content of ghrelin and expression of GHSR mRNA in gastric antrum tissue decreased significantly (P<0.05 or P<0.01) in group B. Compared with group B, blood glucose and urine glucose decreased significantly (P<0.05), GER, the content of insulin in serum, the content of ghrelin and expression of GHSR mRNA in gastric antrum tissue increased significantly (P<0.05 or P<0.01) in group C. Conclusion: EA at acupoints can down-regulate the content of blood and urine sugar, and promote gastric emptying, which is possibly related to the regulation of serum insulin, and the expressions of ghrelin and GHSR mRNA in gastric antrum.
作者机构:
[陈海交; 林亚平; 杨建文; 张程程; 彭艳; 刘薇薇; 刘丽; 刘密; 岳增辉] Hunan University of Chinese Medicine, Changsha, 410208
通讯机构:
[Yan Peng] H;Hunan University of Chinese Medicine, Changsha, China
关键词:
Acupuncture Therapy;Electroacupuncture;Specificity of Acupoints;Point Selection;Diabetes Complications;Gastroparesis;Rats
摘要:
Objective: To observe the effect of electroacupuncture (EA) on the electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis (DGP). Methods: Fifty Sprague-Dawley (SD) rats were randomly divided into group A, group B, group C, group D and group E, 10 rats in each group. Group A was the blank control group without intervention. Group B, Group C, Group D and Group E were treated with single dose intraperitoneal injection of 2% streptozotocin (STZ), combined with 8-week high glucose and high fat diet to establish DGP rat models. Group B was the model group without treatment. Group C was the EA at acupoint group, was treated with EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6). Group D was the EA at non-acupoint group, was treated by EA at the control points of Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6). Rats in the metoclopramide control group received 1.7% metoclopramide solution [10 mL/(kg·bw)] by gavage. Rat's blood glucose level was measured by blood glucose meter; gastric emptying rate was detected using phenol red as a marker; the electrogastrogram was detected by BL-420F biological function system; the protein level of ghrelin was detected by enzyme-linked immunosorbent assay (ELISA); the expression of ghrelin mRNA was detected by real-time polymerase chain reaction (RT-PCR). Results: Compared with group A, the blood glucose of group B, C, D and E were significantly increased before and after the treatment (all P<0.01); after treatment, the gastric emptying rate of group B was significantly decreased (P<0.01), the migration rates of small intestine in group B, C, D and E were significantly lower (all P<0.01), and the protein content of ghrelin in group C was significantly decreased (P<0.01); the expressions of ghrelin mRNA were significantly increased in group B, C, D and E (all P<0.01), the mean amplitudes of electrogastrogram in group B and D were significantly decreased (both P<0.01). After treatment, compared with group B, the blood glucose of group C was significantly decreased (P<0.05), the gastric emptying rate and small intestine migration rate were significantly increased in group C and E (P<0.05, P<0.01), the small intestinal migration rate was significantly increased in group D (P<0.05), the expression of ghrelin in protein and mRNA in group C was significantly lower (P<0.01), the expression of ghrelin mRNA in group E was significantly lower (P<0.05), and the mean amplitude of electrogastrogram in group C was significantly increased (P<0.05). After treatment, compared with group D, the protein and mRNA expressions of ghrelin in group C were significantly decreased (P<0.01). After treatment, compared with group E, the protein expression of ghrelin in group C was significantly decreased (P<0.01). Conclusion: EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) could regulate the blood glucose level of DGP model rats, enhance electrogastrogram activity, promote gastric emptying, and regulate ghrelin expression in protein and mRNA.