作者机构：
[林亚平; 刘琴; 陈楚淘; 陈文; 肖姮; 杨茜芸; 田浩梅] Hunan University of Traditional Chinese Medicine, Changsha, 410208
通讯机构：
[Hao-mei Tian] H;Hunan University of Traditional Chinese Medicine, Changsha, China
关键词：
Acupuncture Therapy;Reperfusion Injury;Hypothermia Induced;Brain Ischemia;Apoptosis;Mitogen-activated Protein Kinases;Rats
摘要：
Objective: To observe the protective effect of acupuncture plus mild hypothermia on brain tissues in rats with cerebral ischemia-reperfusion injury (CIRI), and the influence on protein expression levels of phosphorylated Raf-1, MEK-2 and ERK1/2 in the mitogen-activated protein kinase (MAPK)/extracellular regulated protein kinases (ERK) pathway, and to explore the mechanism of acupuncture plus mild hypothermia therapy for the ischemic stroke. Methods: Ninety Sprague-Dawley (SD) rats were randomly divided into a blank control group, a sham operation group, a model group, an acupuncture group, a mild hypothermia group and an acupuncture plus mild hypothermia group, 15 rats in each group. Except the rats in the blank control group, the remaining rats were used to prepare the middle cerebral artery occlusion (MCAO) models according to the modified occlusion method using lines, while only the occlusion lines were inserted without blocking the brain arteries of rats in the sham operation group. When the vital signs of rats were stable, rats in the blank control group did not receive any intervention; rats in the sham operation group and the model group received fastening without treatment; rats in the acupuncture group, the mild hypothermia group, and the acupuncture plus mild hypothermia group were treated with the corresponding therapeutic methods. 72 h later, observed neurologic injury score, evaluated infarction area ratio by 2,3,5-tripheyl tetrazolium chloride (TTC) staining, determined apoptosis by TUNEL assay, and measured the phosphorylated Raf-1, MEK-2 and ERK1/2 protein expression levels in rat ischemic hippocampal tissues by Western blot assay. Results: Compared with the blank control group and the sham operation group, after modeling, the neurologic injury score, infarction area ratio and apoptotic cells were increased, and phosphorylated Raf-1, MEK-2 and ERK1/2 protein expression levels were significantly increased in the model group; the differences were statistically significant (P<0.05 or P<0.01). Compared with the model group, after acupuncture or mild hypothermia therapy, neurologic injury score and infarction area ratio were decreased; apoptotic cells and phosphorylated Raf-1, MEK-2 and ERK1/2 protein expression levels were significantly decreased; the differences were statistically significant (P<0.05 or P<0.01). Compared with the acupuncture group, neurologic injury score and phosphorylated Raf-1, MEK-2 and ERK1/2 protein expression levels were decreased in the acupuncture plus mild hypothermia group; differences between the groups were statistically significant (P<0.05 or P<0.01). Compared with the mild hypothermia group, phosphorylated Raf-1, MEK-2 and ERK1/2 protein expression levels decreased in the acupuncture plus mild hypothermia group, and differences were statistically significant (P<0.01). Conclusion: Acupuncture or mild hypothermia therapy can improve neurologic injury, reduce infarction area and apoptosis, which brought about protective effect on the brain tissues, in the MCAO model. The protective effect of acupuncture plus mild hypothermia group is the strongest. The mechanism may involve the MAPK/ERK pathway, by reducing the phosphorylated Raf-1, MEK-2 and ERK1/2 protein expression levels.
摘要：
目的：观察电针对糖尿病胃轻瘫(DGP)大鼠血清胰岛素(INS)水平和胃窦组织胆囊收缩素(CCK)含量的影响。方法将60只SD大鼠随机分为A组、B组、C组、D组和E组,每组12只。A组为正常对照组,B组、C组、D组和E组采用腹腔一次性注射2%链脲佐菌素(STZ)配合高糖高脂不规则饮食建立DGP模型。B组为模型组,不接受治疗；C组采用电针足三里、三阴交和梁门治疗；D 组采用电针足三里、三阴交和梁门的对照点治疗；E 组采用甲氧氯普胺片(胃复安)药液灌胃治疗。采用OneTouch 血糖仪测血糖,尿糖试纸测尿糖,酚红灌胃法测胃排空率和肠移行率,并采用 ELISA 法检测各组大鼠治疗前后血清INS水平和胃窦组织CCK的含量。结果 B组、C组、D组和E组大鼠血糖值及尿糖值与A组比较,差异均具有统计学意义(P＜0.01)。C组大鼠血糖值及尿糖值与B组和E组比较,差异均具有统计学意义(P＜0.05)。B组、C组、D组和E组大鼠胃排空率与 A 组比较,差异均具有统计学意义(P＜0.01)。C 组大鼠胃排空率与 B 组和 D 组比较,差异均具有统计学意义(P＜0.01,P＜0.05)。B组和D组大鼠肠移行率与A组比较,差异均具有统计学意义(P＜0.01)。C组大鼠肠移行率与B组和D组比较,差异均具有统计学意义(P＜0.01)。B组大鼠血清INS水平及胃窦组织CCK含量与A组比较,差异均具有统计学意义(P＜0.05,P＜0.01)。D组大鼠胃窦组织CCK含量与A组比较,差异具有统计学意义(P＜0.01)。C组和E组大鼠血清INS水平及胃窦组织CCK含量与B组比较,差异均具有统计学意义(P＜0.05,P＜0.01)。C组大鼠胃窦组织CCK含量与D组比较,差异具有统计学意义(P＜0.05)。结论电针能明显改善DGP大鼠的胃肠功能症状,促进胃排空,其疗效可能与电针刺激能提高血清INS水平,同时降低胃窦组织CCK含量有关。
期刊：
Journal of Acupuncture and Herbs,2015年3(1):70-76
作者机构：
Graduate School, Hunan University of Traditional Chinese Medicine, Changsha, 410208, China Key Acu-Moxibustion Lab. of Biological Information Analyzing, Institute of Acupuncture, Moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha, 410208, China
关键词：
Life Sciences;Life Sciences, other;Medicine;Clinical Medicine;Complementary Medicine;Pharmacy;Pharmacy, other
摘要：
Objective: To observe the effects of electro-acupuncture (EA) on ultrastructure of gastric antrum smooth muscle cells (GASMCs) in diabetic gastroparesis (DGP) model rats, and to explore the possible mechanism underlying the therapeutic effects of EA.
期刊：
Journal of Acupuncture and Herbs,2015年3(1):38-45 ISSN：2001-7464
作者机构：
Key Research Lab. on Acupoint-organ Relations and Key Lab. of Bioinformatic Analysis on Acupuncture-moxibustion, Acupuncture-Moxibustion and Tuina Massage School, Hunan University of Traditional Chinese Medicine, Changsha, 417000, China Phisiotherapy Department, Hunan People’s Hospital, Changsha, 410005, China
关键词：
Life Sciences;Life Sciences, other;Medicine;Clinical Medicine;Complementary Medicine;Pharmacy;Pharmacy, other
摘要：
Objective: to observe the effects of moxibustion on histomorphological changes of gastric mucosa, as well as on serum IL-6、IL-8、TNF-α，Hp IgG、CD Methods: 40 SD rats were randomly assigned to four groups: group A (blank control), group B (Hp infection model), group C (moxibustion plus model), group D (electro-acupuncture plus model), 10 for each group. The “NaHCO Results: compared with group A, the values of IL-6, IL-8, TNF-α, Hp IgG and CD Conclusion: the preventive and therapeutic effects of moxibustion on Hp related gastritis might be realized by two ways- to inhibit the secretion of proinflammatory cytokines such as IL-6, IL-8 and TNF-α, or to regulate the production of immune factors (such as up-regulation of CD
摘要：
目的:观察针刺与西药对比治疗阴虚阳亢型原发性高血压病的临床疗效差异。方法:将符合纳入标准的60例原发性高血压病患者随机分为针刺组、药物组各30例。针刺组分为2组取穴:①四关穴,②曲池、行间,两组穴位交替使用,每天针1次;药物组予口服卡托普利片,每次25 mg,每日3次。两组均7天为一疗程,治疗3个疗程。结果:(1)两组在治疗后血压都有下降,前后差异均具有统计学意义(均P<0.01),治疗14天、21天后针刺组降低舒张压效果明显优于药物组[(83.60±7.56)mmHg vs (89.76±6.51)mmHg,(76.60±4.13)mmHg vs (90.93±7.94)mmHg,均P<0.01];(2)两组中医症候积分比较,针刺组对头晕、腰膝酸软、心悸及总积分值改善情况明显优于药物组(均P<0.05);(3)针刺组与药物组比较,发生不良反应例数少,差异有统计学意义(P<0.05)。结论:针刺四关、曲池、行间治疗原发性高血压病疗效确切,对舒张压的控制作用优于卡托普利,且能较好地改善头晕、腰膝酸软、心悸等临床症状。
作者机构：
[Yan Peng; Shou-xiang Yi; Fen Peng; Yi-wen Long; Ya-ping Lin; Xiao-rong Chang] Major Laboratory of Meridians and Viscera, Tertiary Laboratory of State Administration of Traditional Chinese Medicine, Institute of Acupuncture, Moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha, China;[Bin Xu] Traditional Chinese Medicine Department of Nanjing University of Chinese Medicine, Nanjing, China
通讯机构：
[Bin Xu] T;Traditional Chinese Medicine Department of Nanjing University of Chinese Medicine, Nanjing, China
