摘要:
The processing of medicinal materials, known as Pao Zhi in traditional Chinese medicine, is a unique part of traditional Chinese medicine and has been widely used for the preparation of Chinese materia medica. It is believed that processing can alter the properties and functions of remedies, increase medical potency, and reduce toxicity and side effects. Both processed and unprocessed Flos Lonicerae (flowers of Lonicera japonica) are important drug ingredients in traditional Chinese medicine. To gain insights on the effect of processing factors (heating temperature and duration) on the change of chemical composition, nuclear magnetic resonance combined with chemometric analysis was applied to investigate the processing of F. Lonicerae. Nuclear magnetic resonance spectral data were analyzed by means of a heat map and principal components analysis. The results indicated that the composition changed significantly, particularly when processing at the higher temperature (210 °C). Five chemical components, viz. 3,4-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, chlorogenic acid, and myo-inositol, whose concentration changed significantly during the processing, were isolated and identified. The patterns for the concentration change observed from nuclear magnetic resonance analysis during the processing were confirmed and quantitatively determined by ultrahigh-performance liquid chromatography analysis. The study demonstrated that a nuclear magnetic resonance-based chemometric approach could be a promising tool for investigation of the processing of herbal medicines in traditional Chinese medicine.
作者:
Long Mian*;Li Shun-xiang;Xiao Jiang-feng;Wang Jian;Scott, Lozanoff;...
期刊:
中国结合医学杂志,2014年20(9):675-681 ISSN:1672-0415
通讯作者:
Long Mian
作者机构:
[Long Mian] Univ Hawaii Manoa, Dept Complementary & Alternat Med, John A Burns Sch Med, Honolulu, HI 96813 USA.;[Li Shun-xiang] Hunan Acad Tradit Chinese Med, Dept Phytochem, Inst Chinese Medica Matria, Changsha 410013, Hunan, Peoples R China.;[Wang Jian; Xiao Jiang-feng] Jiangsu Jiangshan Pharmaceut Co, Dept Res, Jingjiang 214500, Jiangsu, Peoples R China.;[Scott, Lozanoff] Univ Hawaii Manoa, Dept Anat Biochem & Physiol, John A Burns Sch Med, Honolulu, HI 96813 USA.;[Zhang Zhi-guang] Hunan Normal Univ, Sch Life Sci, Dept Bot, Changsha 410006, Hunan, Peoples R China.
通讯机构:
[Long Mian] U;Univ Hawaii Manoa, Dept Complementary & Alternat Med, John A Burns Sch Med, Honolulu, HI 96813 USA.
关键词:
kaempferol;renal tubular cell;osteoblast growth factor;Rhizoma Drynariae;bone morphological;protein receptor;Kidney controls the bone
摘要:
OBJECTIVE: To study, at the cytological level, the basic concept of Chinese medicine that "the Kidney (Shen) controls the bone". METHODS: Kaempferol was isolated form Rhizoma Drynariae (Gu Sui Bu, GSB) and at several concentrations was incubated with opossum kidney (OK) cells, osteoblasts (MC3T3 E1) and human fibroblasts (HF) at cell concentrations of 2x10(4)/mL. Opossum kidney cell-conditioned culture media with kaempferol at 70 nmol/L (70kaeOKM) and without kaempferol (0OKM) were used to stimulate MC3T3 E1 and HF proliferation. The bone morphological protein receptors I and II (BMPR I and II) in OK cells were identified by immune-fluorescence staining and Western blot analysis. RESULTS: Kaempferol was found to increase OK cell growth (P<0.05), but alone did not promote MC3T3 E1 or HF cell proliferation. However, although OKM by itself increased MC3T3 E1 growth by 198% (P<0.01), the 70kaeOKM further increased the growth of these cells by an additional 127% (P<0.01). It indicates that the kidney cell generates a previously unknown osteoblast growth factor (OGF) and kaempferol increases kidney cell secretion of OGF. Neither of these media had any significant effect on HF growth. Kaempferol also was found to increase the level of the BMPR II in OK cells. CONCLUSIONS: This lends strong support to the original idea that the Kidney has a significant influence over bone-formation, as suggested by some long-standing Chinese medical beliefs, kaempferol may also serve to stimulate kidney repair and indirectly stimulate bone formation.
摘要:
Background: Bushen Huoxue Qubi (BHQ) granules, a traditional Chinese medicine preparation, has been clinically used for the treatment of the blood stasis syndrome. Objective: The main objective is to investigate whether the diseased condition would alter the pharmacokinetics and tissue distribution of tanshinone IIA in BHQ, which was given orally to the acute blood stasis rats. Materials and Methods: The main bioactive constituent in BHQ, tanshinone IIA, was measured in the plasma and tissues of animals by the high performance liquid chromatography with ultraviolet detection. The analysis was successfully performed on an Agilent TC-C-18 column (250 x 4.6 mm I.D., 5 mu m) protected with a Octadecylsilane (ODS) guard column (10 x 4.6 mm I.D., 5 mu m). The mobile phase was aqueous solution (A) (containing 0.40% aqueous acetic acid) and acetonitrile (B). The conditions of the solvent gradient elution were 35-40% (B) in 0-15 min, 40-42% (B) in 15-18 min and 42-70% (B) in 18-30 min at a flow rate of 1.0 mL/min. Detection was conducted with wavelength of 270 nm at 30 degrees C. Results: Good linearity relationships were found (r(2) > 0.9955) over the investigated concentration range for bio-samples. Blood stasis was associated with significantly higher area under the concentration-time curve (AUC), the maximum plasma concentration (C-max) and biological half-life (t(1/2)), lower total body clearance (CL) and apparent volume of distribution (Vd) of tanshinone IIA in plasma and higher AUC(0-t) of tanshinone IIA in the analyzed tissues of rats treated with BHQ. Conclusion: Blood stasis could alter pharmacokinetics and tissue distribution of tanshinone IIA in BHQ.