期刊论文

Establishment and Evaluation of the Combination of Disease And Syndrome Experimental Model of Spleen Stomaeh Deficieney Cold Type of Gastric Ulcer in Rats

中文

中国中医基础医学杂志

1006-3250

2020

26

4

468-473

国家自然科学基金资助项目(81703920)-基于Toll样受体信号通路“危险模式”效应理论解析温中益气健脾法治疗Hp相关性溃疡的分子机制 中国博士后科学基金资助项目(2019M662790) 湖南省自然科学基金青年科学基金项目(2019JJ50442) 湖南中医药大学中医学一流学科开放基金项目(2018ZYX20) 湖南中医药大学校级科研基金项目(2018XJJJ28) 湖南中医药大学青年教师科研基金项目(201610)。

本校为第一机构

目的:建立脾胃虚寒型胃溃疡(GU)病证结合大鼠实验模型并对其进行客观评价。方法: 36只SD大鼠按随机数字方法分为正常组、模型组和黄芪建中汤组(HQJZT) 3组,采用“苦寒泻下+劳倦过度”法联合“乙醇+阿司匹林”造模,观察大鼠的一般症状与体征,测定体质量、进食量、饮水量、肛温,计算溃疡指数,HE染色观察黏形态学改变,ELISA法测定NO、MDA、PGE含量,免疫组化检测TLR-2、MyD88蛋白表达。结果:与正常组比较,模型大鼠体质量、进食量、肛温均显著降低,总体或局部症状与体征、胃黏膜形态均发生病性改变。给予HQJZT后上述指标均明显提高或明显改善,与正常组比较,模型大鼠胃黏膜PGE、NO含量显著降低,MDA含量...

Objective: To establish and evaluate an experimental model of gastric ulcer (GU) in rats with syndrome of spleen-stomach deficiency cold and to evaluate it objectively. Methods: SD rats were randomly divided into normal group, model group,and Huang-Qi-Jian-Zhong-Tang (HQJZT) group. The animal model was established by using method of " bitter cold induced diarrhea + overfatigue " combined with" ethanol + aspirin". The general symptoms and signs of the rats were observed. Body weight,food intake,water intake,and rectal temperature were measured. The degree of mucosal damage was observed and the ...