作者：
Min Zhang;Ling Zhang;Shitao Fang;Yun Wang;Jinkun Guo;...
期刊：
Journal of Orthopaedics,2025年67:132-139 ISSN：0972-978X
通讯作者：
Lei Mi
作者机构：
[Lei Mi; Mi, Lei; Jinkun Guo; Min Zhang] Hunan Univ Chinese Med, Clin Med Coll, Peoples Hosp Hunan Prov 2, Dept Orthopaed Med Ctr,Brain Hosp Hunan Prov, Changsha 410007, Peoples R China.;[Ling Zhang] Cent South Univ, Xiangya Hosp 3, Dept Nursing, Changsha 410013, Peoples R China.;[Shitao Fang] Hanshou Cty Peoples Hosp, Dept Orthopaed, Changde 415999, Peoples R China.;[Yun Wang] Shandong First Med Univ, Affiliated Hosp 1, Dept Surg, Jinan 250013, Peoples R China.
通讯机构：
[Mi, L ] H;Hunan Univ Chinese Med, Clin Med Coll, Peoples Hosp Hunan Prov 2, Dept Orthopaed Med Ctr,Brain Hosp Hunan Prov, Changsha 410007, Peoples R China.
关键词：
Hallux valgus;Scarf osteotomy;Precision treatment;Finite element analysis
摘要：
Background The treatment of hallux valgus is currently in face of many challenges in clinical practice. Although conventional surgical procedures can correct deformities to a certain extent, some key parameters such as osteotomy displacement and angle are often difficult to determine, leading to increased uncertainty in surgical outcomes and a relatively high incidence of postoperative complications.
The treatment of hallux valgus is currently in face of many challenges in clinical practice. Although conventional surgical procedures can correct deformities to a certain extent, some key parameters such as osteotomy displacement and angle are often difficult to determine, leading to increased uncertainty in surgical outcomes and a relatively high incidence of postoperative complications.
Objective Hallux valgus is a common foot deformity that often leads to pain, difficulty in walking, and other health problems, seriously affecting the quality of life of patients. In order to provide patients with more precise treatment, we aimed to simulate the Scarf osteotomy for the treatment of hallux valgus using the finite element method, and comparatively analyze the mechanical performance indicators under different distal osteotomy displacements. We hoped to provide scientific and reasonable treatment plans for clinical practice and better treatment outcomes for patients.
Hallux valgus is a common foot deformity that often leads to pain, difficulty in walking, and other health problems, seriously affecting the quality of life of patients. In order to provide patients with more precise treatment, we aimed to simulate the Scarf osteotomy for the treatment of hallux valgus using the finite element method, and comparatively analyze the mechanical performance indicators under different distal osteotomy displacements. We hoped to provide scientific and reasonable treatment plans for clinical practice and better treatment outcomes for patients.
Methods A volunteer with moderate hallux valgus was selected as the research subject in this study. Preoperative plantar pressure test was conducted, and the CT data of the patient's affected foot was collected to create the finite element model using finite element software. After verifying the validity of the model, it was used to simulate the translational Scarf osteotomy under different settings. Specifically, the distal end of the bone fragment was pushed outwards by varying distances (2 mm, 4 mm, 6 mm, and 8 mm) and was fixed with screws. The maximum Von Mises stresses on the first to fifth metatarsal bones, the sole and the heel under four different surgical settings were compared with the preoperative values, and the changes in the maximum Von Mises stress on the screws were examined.
A volunteer with moderate hallux valgus was selected as the research subject in this study. Preoperative plantar pressure test was conducted, and the CT data of the patient's affected foot was collected to create the finite element model using finite element software. After verifying the validity of the model, it was used to simulate the translational Scarf osteotomy under different settings. Specifically, the distal end of the bone fragment was pushed outwards by varying distances (2 mm, 4 mm, 6 mm, and 8 mm) and was fixed with screws. The maximum Von Mises stresses on the first to fifth metatarsal bones, the sole and the heel under four different surgical settings were compared with the preoperative values, and the changes in the maximum Von Mises stress on the screws were examined.
Results When the distal end of the bone fragment was pushed outwards by 6 mm, the maximum Von mises stress on the first metatarsal bone (9.4711 MPa) reached its highest value, while the maximum Von mises stresses on the second and third metatarsal bones (0.34062 MPa and 1.6246 MPa, respectively) and on the screws (40.99 MPa) were at the lowest values. The maximum plantar pressure detected during static test was 0.292 MPa, while the maximum plantar stress observed in the finite element model was 0.25733 MPa, indicating comparable values between the two.
When the distal end of the bone fragment was pushed outwards by 6 mm, the maximum Von mises stress on the first metatarsal bone (9.4711 MPa) reached its highest value, while the maximum Von mises stresses on the second and third metatarsal bones (0.34062 MPa and 1.6246 MPa, respectively) and on the screws (40.99 MPa) were at the lowest values. The maximum plantar pressure detected during static test was 0.292 MPa, while the maximum plantar stress observed in the finite element model was 0.25733 MPa, indicating comparable values between the two.
Conclusions Based on precise and customized preoperative design and finite element analysis, it was found that the Scarf osteotomy with the distal end of the bone fragment pushed outwards for 6 mm could yield the best treatment effect for hallux valgus. Under this setting, the stress on the first metatarsal bone was the highest, while the stresses on the second and third metatarsal bones were the lowest, suggesting that it can relieve the stress on lateral metatarsal bones and improve the stress distribution of the forefoot. Overall, Scarf osteotomy under 6 mm setting can effectively correct hallux valgus deformity, reduce the incidence of metastatic plantar pain and prevent the recurrence of metastatic plantar pain after surgery. By improving the patient's plantar stress distribution, this surgical setting is expected to provide better clinical outcomes.
Based on precise and customized preoperative design and finite element analysis, it was found that the Scarf osteotomy with the distal end of the bone fragment pushed outwards for 6 mm could yield the best treatment effect for hallux valgus. Under this setting, the stress on the first metatarsal bone was the highest, while the stresses on the second and third metatarsal bones were the lowest, suggesting that it can relieve the stress on lateral metatarsal bones and improve the stress distribution of the forefoot. Overall, Scarf osteotomy under 6 mm setting can effectively correct hallux valgus deformity, reduce the incidence of metastatic plantar pain and prevent the recurrence of metastatic plantar pain after surgery. By improving the patient's plantar stress distribution, this surgical setting is expected to provide better clinical outcomes.
期刊：
Frontiers in Pharmacology,2025年16:1578400 ISSN：1663-9812
通讯作者：
Yu, R
作者机构：
[Zhou, Tongyi; Liu, Xiu; Zou, Junju; Li, Liu; Tan, Danni; Yu, Rong; Xiang, Qin; Yu, R] Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Peoples R China.;[Liu, Xiu; Yu, Rong; Yu, R] Hunan Univ Chinese Med, Hunan Key Lab Tradit Chinese Med Prescript & Syndr, Changsha, Peoples R China.
通讯机构：
[Yu, R ] H;Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Peoples R China.;Hunan Univ Chinese Med, Hunan Key Lab Tradit Chinese Med Prescript & Syndr, Changsha, Peoples R China.
关键词：
autophagy;diabetic kidney disease;mTOR;mechanism;oxidative stress;sense of nutrition;traditional Chinese medicine
摘要：
CONTEXT: Autophagy plays a pivotal role in the pathogenesis of DKD, and the mechanistic target of rapamycin (mTOR) pathway, which regulates nutrient sensing and oxidative stress responses, is a key regulator of autophagy. Traditional Chinese Medicine (TCM) has garnered attention for its potential to treat DKD by modulating the mTOR signaling pathway, reducing oxidative stress, and restoring autophagic function. OBJECTIVE: The objective of this study is to examine how mTOR-mediated regulation of nutrient sensing and oxidative stress impacts autophagy in DKD, and to explore how TCM modulates these pathways to improve the condition. METHODS: A systematic review was conducted using PubMed, Web of Science, Wanfang Data, and China National Knowledge Infrastructure (CNKI), with the search extended to December 2024. The search subject terms included 'diabetic kidney disease,' 'Traditional Chinese Medicine,' 'mTOR,' 'nutrient sensing,' and 'oxidative stress.' Studies were rigorously screened by two investigators. RESULTS: This review systematically examines the pathogenesis of mTOR-mediated nutrient sensing dysfunction and oxidative stress in DKD, highlighting their impact on autophagy. It further clarifies how these mechanisms are targeted by Chinese medicine in the treatment of DKD. The review summarizes the potential mechanisms by which TCM, including monomers (e.g., Astragaloside IV), individual botanical drugs (e.g., Dendrobium nobile Lindl.), and compound formulations (e.g., Tongluo Digui Decoction), regulate autophagy in DKD through pathways such as AMP-activated protein kinase (AMPK), mTOR, sirtuins (Sirt), and the phosphatidylinositol three kinase (PI3K)/Akt/mTOR signaling pathway. TCM compound formulas share a common foundational framework, with the majority being formulated based on therapeutic principles such as 'Yiqi', 'Yangyin', 'Tongluo', and 'Huashi'. CONCLUSION: TCM shows promise in treating DKD, with unique advantages in modulating key signaling pathways. However, the underlying mechanisms remain complex and warrant further investigation.
摘要：
The human skin microbiome is closely associated with various diseases. We aimed to find the causal association of the human skin microbiome with vaginitis. A two-way two-sample Mendelian randomization study used summary statistics of the human skin microbiota from the largest genome-wide association study meta-analysis. Pooled statistics for vaginitis disease were obtained from the FinnGen consortium R10 release. Inverse variance weighted, MR-Egger, weighted median, weighted model, MR-PRESSO, and STEIGER methods were used to test for causal associations between different parts of the human skin microbiota and vaginitis disease in either dry or moist environments. IVW estimates suggest that phylococcus hominischaracterizes a protective effect against vaginitis lesions at the Antecubitalfossa wet site. Staphylococcus in the dry state at the Dorsalforearm site also demonstrated a protective effect against vaginitis lesions. Micrococcus showed a trend of up-regulation of vaginitis risk under IVW method estimation. In contrast, Anaerococcus and Veillonella were associated with a low risk of vaginitis as estimated by the IVW method: ASV061 and ASV065. In the Class, IVW estimation showed that Bacilli at Antecubitalfossa site had an increased risk effect on vaginitis in the moist skin condition. Similarly in Genus, Staphylococcus at Antecubitalfossa sites had an increased risk effect against vaginitis in the moist skin condition. Based on the results of the inverse MR analysis, no significant causal effect of vaginitis on the human skin microbiota was found. This two-sample bidirectional Mendelian randomization study found a causal relationship between seven human skin microbiota and vaginitis, further enriching our understanding of the value of skin flora in reproductive diseases such as vaginitis.
作者机构：
[Zhang, JP; Li, Juan; Zhang, Jing-Ping] Cent South Univ, Xiangya Sch Nursing, Changsha, Peoples R China.;[Li, Chan] Guangzhou Univ Chinese Med, Clin Med Coll 5, Guangzhou, Peoples R China.;[Li, Chan] Yeahcome Hlth Ind Grp Co Ltd, Changsha, Peoples R China.;[Zou, Jie] Chongqing Univ Canc Hosp, Hepatobiliary Pancreat Oncol Ctr, Chongqing Key Lab Translat Res Canc Metastasis & I, Chongqing, Peoples R China.;[Cheng, Fang-Qun] Xiangtan Cent Hosp, Dept Nursing, Xiangtan, Peoples R China.
通讯机构：
[Zhang, JP ] C;[Chen, OY ] H;Cent South Univ, Xiangya Sch Nursing, Changsha, Peoples R China.;Hunan Univ Tradit Chinese Med, Sch Nursing, Changsha, Peoples R China.
关键词：
Lung cancer patients;Caregivers;Psychological stress;Prevalence;Influencing factors;Anxiety;Depression
摘要：
A lung cancer diagnosis has a huge impact on the psychological well-being of both patients and family caregivers. However, the current psychological stress status among dyads remains unclear. We aimed to determine the prevalence of anxiety and depression and identify the factors that influence patients with lung cancer and their caregivers. We conducted a cross-sectional study of 254 dyads of lung cancer patients and family caregivers from four tertiary hospitals in Hunan Province, China from January 2021 to June 2021. Besides, we used several instruments to collect data on depression, anxiety, illness perception, mindfulness, self-compassion, and dyadic coping. The independent samples t-test, analysis of one-way variance, Spearman’s correlation analysis, and multiple linear regression analysis were employed. Results showed that 21.3% of patients and 29.5% of caregivers experienced depression, while 22.0% of patients and 33.5% of caregivers reported anxiety. For lung cancer patients, patients’ illness perception positively predicted their depression scores and patients’ level of mindfulness and level of self-compassion negatively predicted their depression scores; patients’ illness perception positively predicted their anxiety scores, and only living with children, patients’ mindfulness and self-compassion levels were negatively predicted their anxiety scores. For caregivers, caregivers’ level of illness perception and the number of other caregivers positively predicted their depression and anxiety scores, and caregiver’s dyadic coping level, residence (county or town, and city), and caregiver’s self-compassion level, were negatively predicted their depression and anxiety scores. The prevalence of psychological stress response of lung cancer patients and caregivers was high and influenced by family living situation, number of persons assisting in care, place of residence, patient-caregiver relationship, levels of illness perception, mindfulness, self-compassion, and dyadic coping. ClinicalTrial.gov NCT06746948.
期刊：
MOLECULES AND CELLS,2025年48(6):100216 ISSN：1016-8478
通讯作者：
Yu, R
作者机构：
[Yu, Yun-Feng; Qin, Li-Na; Yu, Rong; Yu, R] Hunan Univ Chinese Med, Dept Endocrinol, Hosp 1, Changsha, Hunan, Peoples R China.;[Ma, Lie] Henan Univ Chinese Med, Dept Reprod Med, Affiliated Hosp 3, Zhengzhou, Henan, Peoples R China.;[Yu, Rong; Yu, R] Hunan Univ Chinese Med, Coll Chinese Med, Changsha, Hunan, Peoples R China.;[Yu, Rong; Yu, R] Hunan Univ Chinese Med, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Yu, R ] H;Hunan Univ Chinese Med, Dept Endocrinol, Hosp 1, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Coll Chinese Med, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.
摘要：
Metabolic dysfunction-associated steatotic liver disease (MASLD) is a progressive disease that affects the health of approximately one-third of the world's population. It is the primary cause of end-stage liver disease, liver malignancy, and liver transplantation, resulting in a great medical burden. No medications have yet been approved by the US Food and Drug Administration (FDA) for treating MASLD without liver inflammation or scarring. Therefore, the development of specific drugs to treat MASLD remains a key task in the ongoing research objective. Extracellular vesicles (EVs) play an important role in the communication between organs, tissues, and cells. Recent studies have found that intestinal microbiota are closely related to the pathogenesis and progression of MASLD. EVs produced by bacteria (BEVs) play an indispensable role in this process. Thus, this study provides a new direction for MASLD treatment. However, the mechanism by which BEVs affect MASLD remains unclear. Therefore, this study investigated the influence and function of intestinal microbiota in MASLD. Additionally, we focus on the research progress of BEVs in recent years, and explain the relationship between BEVs and MASLD from the perspectives of glucose and lipid metabolism, immune responses, and intestinal homeostasis. Finally, we summarized the potential therapeutic value of BEVs and EVs from other sources, such as adipocytes, immunocytes, stem cells, and plants.
Metabolic dysfunction-associated steatotic liver disease (MASLD) is a progressive disease that affects the health of approximately one-third of the world's population. It is the primary cause of end-stage liver disease, liver malignancy, and liver transplantation, resulting in a great medical burden. No medications have yet been approved by the US Food and Drug Administration (FDA) for treating MASLD without liver inflammation or scarring. Therefore, the development of specific drugs to treat MASLD remains a key task in the ongoing research objective. Extracellular vesicles (EVs) play an important role in the communication between organs, tissues, and cells. Recent studies have found that intestinal microbiota are closely related to the pathogenesis and progression of MASLD. EVs produced by bacteria (BEVs) play an indispensable role in this process. Thus, this study provides a new direction for MASLD treatment. However, the mechanism by which BEVs affect MASLD remains unclear. Therefore, this study investigated the influence and function of intestinal microbiota in MASLD. Additionally, we focus on the research progress of BEVs in recent years, and explain the relationship between BEVs and MASLD from the perspectives of glucose and lipid metabolism, immune responses, and intestinal homeostasis. Finally, we summarized the potential therapeutic value of BEVs and EVs from other sources, such as adipocytes, immunocytes, stem cells, and plants.
摘要：
Heart failure (HF) is a complex syndrome marked by considerable expenditures and elevated mortality and morbidity rates globally. Shenmai injection (SMI), a form of Traditional Chinese Medicine-based therapy, has demonstrated effectiveness in treating HF. Recent research suggests that Traditional Chinese Medicine (TCM) may induce beneficial changes in microbial-host co-metabolism, potentially providing cardiovascular protection. This study used a rat model of hypertensive heart failure (H-HF) to explore the mechanism of SMI. The possible compounds and key targets of SMI against H-HF were investigated using network pharmacology. The pharmacodynamics of SMI were validated using the H-HF animal model, with analysis of fecal gut microbiota integrating metabolomics and 16S rRNA sequencing. Metorigin metabolite traceability analysis and the MetaboAnalyst platform were utilized to explore the action mechanism. To evaluate changes in serum TMAO levels, targeted metabolomics was performed. Finally, the study looked at the intrinsic relationships among modifications in the intestinal flora, metabolite profile changes, and the targets of SMI compounds to clarify how they might be used to treat H-HF. According to metabolomics and 16S rRNA sequencing, by reestablishing homeostasis in the gut microbiota, SMI affects vital metabolic pathways, such as energy metabolism, amino acid metabolism, and bile acid metabolism. Increased serum TMAO levels were identified to be a risk factor for H-HF, and SMI was able to downregulate the levels of TMAO-related metabolites. Network pharmacology analysis identified 13 active components of SMI targeting 46 proteins, resulting in differential expression changes in 8 metabolites and 24 gut microbes. In conclusion, this study highlights the effectiveness of SMI in alleviating H-HF and its potential to modulate microbial-host co-metabolism. Through a comprehensive discussion of the interconnected relationships among the components, targets, metabolites, and gut microbiota, it provided fresh light on the therapeutic mechanism of SMI on H-HF.
作者机构：
[Dai, Jie; Huang, Shiyi; Liu, Shi Ao; Zhao, Hongqing; Zou, Ting; He, Xiangchang; He, Weihe; Xu, Guangming] Hunan Univ Chinese Med, Sch Pharm, Changsha, Peoples R China.
通讯机构：
[Xu, GM ] H;Hunan Univ Chinese Med, Sch Pharm, Changsha, Peoples R China.
摘要：
Traditional Chinese Medicine (TCM) holds significant potential as a natural source of medication, providing effective alternatives for preventing and treating thrombotic diseases. Although research on the anticoagulant activity of Agrimonia Pilosa Ledeb (Rosaceae, A. pilosa) has been paid attention to, the monomer component and its mechanism of action have not been studied deeply. Therefore, this study aimed to screen and identify the antithrombotic active components of A. pilosa by affinity ultrafiltration (AUF) combined with ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). The affinity between compounds and thrombin was studied through molecular docking, and the activity of thrombin inhibitors was evaluated from multiple dimensions by the zebrafish thrombus model, chromogenic substrate method, and coagulation factor test. Eleven ligands with potential antithrombotic activity were screened from A. pilosa. Among them, compounds hyperoside, quercitrin, afzelin, baicalin, quercetin, kaempferol, and 3-O-caffeoylquinic acid were shown to have antithrombotic activity. This study is the first to report that kaempferol-3-rhamno-glucoside, tiliroside, and taxifolin exhibit thrombin-inhibitory effects through both in vivo and in vitro experiments. The results of this study indicate that AUF-HPLC and UPLC-Q-TOF-MS can be used for high-throughput screening of active components from TCM, thus laying a groundwork for the discovery of thrombin inhibitors and promoting the research progress of antithrombotic drugs.
期刊：
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2025年15:1539277 ISSN：2235-2988
通讯作者：
Peng, XX;Deng, Na;Deng, N
作者机构：
[Peng, Xinxin; Peng, XX; Fang, Leyao] Hunan Univ Chinese Med, Hosp 1, Changsha, Peoples R China.;[Shen, Junxi; Peng, Xinxin; Yi, Xin; Deng, Na; Peng, XX; Deng, N; Fang, Leyao] Hunan Univ Chinese Med, Domest Class Discipline Construction Project Chine, Changsha, Peoples R China.;[Shen, Junxi; Yi, Xin; Deng, N; Deng, Na; Fang, Leyao] Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Peoples R China.
通讯机构：
[Deng, N ; Peng, XX ; Deng, N] H;Hunan Univ Chinese Med, Hosp 1, Changsha, Peoples R China.;Hunan Univ Chinese Med, Domest Class Discipline Construction Project Chine, Changsha, Peoples R China.;Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Peoples R China.
摘要：
BACKGROUND: Constipation is a common digestive system disorder, which is closely related to the intestinal flora. Zhishi Daozhi decoction (ZDD) is a traditional Chinese medicine prescription used to treat constipation caused by indigestion. This study is to evaluate the efficacy of ZDD in treating constipation and to elucidate the underlying mechanism. METHODS: In this study, Kunming mice were administered a high-protein diet (HFHPD) and loperamide hydrochloride injections to induce constipation. The mice then received varying doses (2.4, 4.7, and 9.4 mg/kg) of ZDD for seven days. Following the sampling process, we measured fecal microbial activity. The levels of 5-hydroxytryptamine (5-HT), vasoactive intestinal peptide (VIP), and aquaporin-3 (AQP3) were quantified using enzyme-linked immunosorbent assay. Changes in the gut microbiota were evaluated through 16S rRNA gene sequencing. Additionally, we investigated the correlation between specific microbiota features and the levels of 5-HT, VIP, and AQP3. RESULTS: The fecal surface of the mice in the model group (CMM) was rough and dry. The stool of mice in the low-dose ZDD group (CLD), medium-dose ZDD group (CMD), and high-dose ZDD group (CHD) exhibited a smoother texture, closely resembling that of the normal group (CNM). 5-HT levels in the CMM group were significantly lower than in the CNM, CLD, and CHD. VIP levels in the CMD were lower than in the other four groups, and AQP3 levels in CMM showed a decreasing trend. The fecal microbial activity of the CMM group was significantly higher than that of the other groups. Diversity analysis indicated that CMD and CHD treatments were more effective in restoring the intestinal microbiota structure. Potential pathogenic bacteria, including Clostridium, Aerococcus, Jeotgalicoccus, and Staphylococcus were enriched in CMM. In contrast, beneficial bacteria such as Faecalibacterium, Bacillaceae, and Bacillus were more prevalent in the CLD, CMD, and CHD. Correlation analysis revealed that Streptococcus and Enterococcus were positively correlated with VIP, while Succinivibrio showed a negative correlation with 5-HT. CONCLUSIONS: Constipation induced by HFHPD and loperamide hydrochloride disrupts the structure of the intestinal microbiota. ZDD appears to alleviate constipation, potentially through mechanisms linked to the brain-gut axis and its interaction with the intestinal microbiota. Among the treatment groups, the medium dose of ZDD demonstrated the most effective results.
期刊：
Journal of Applied Toxicology,2025年 ISSN：0260-437X
通讯作者：
He, X
作者机构：
[Guo, Bing] Hunan Univ Chinese Med, Grad Sch, Changsha, Hunan, Peoples R China.;[He, Xuan; He, X] Changsha Med Univ, Coll Tradit Chinese Med, Changsha, Hunan, Peoples R China.
通讯机构：
[He, X ] C;Changsha Med Univ, Coll Tradit Chinese Med, Changsha, Hunan, Peoples R China.
摘要：
The increasing prevalence of environmental pollutants has raised public concern about their potential role in diseases such as atherosclerosis (AS). Existing studies suggest that chemicals, including bisphenol S (BPS), may adversely affect cardiovascular health, but the specific mechanisms remain unclear. This study aims to elucidate the effects of BPS on AS and the underlying mechanisms. Through an extensive search of databases such as ChEMBL, STITCH, SwissTargetPrediction, SuperPred, SEA, and GEO, we identified 34 potential targets related to BPS-induced AS. A target network was constructed using the STRING platform and Cytoscape software. GO and KEGG functional enrichment analysis using the DAVID database revealed that BPS may promote the occurrence of AS by interfering with critical biological processes such as glutathione metabolism, nitrogen metabolism, and tyrosine metabolism. This was followed by the selection of 4 core targets-aminopeptidase n (ANPEP), alcohol dehydrogenase 5 (ADH5), lysosomal pro-x carboxypeptidase (PRCP), and microsomal glutathione s-transferase 1 (MGST1)-using five machine learning methods. These core targets play a pivotal role in BPS-induced AS. Furthermore, molecular docking confirmed the tight binding between BPS and these core targets. In conclusion, this study provides a theoretical framework for understanding the molecular mechanisms of BPS-induced AS and contributes scientific evidence for the development of prevention and treatment strategies for cardiovascular diseases triggered by BPS exposure.
摘要：
BACKGROUND AND OBJECTIVES: To explore the mechanism of action of the differential components of medicinal and edible lilies in treating depression by network pharmacology using UPLC-Q-TOF-MS technology. METHODS: The chemical composition of medicinal and edible lilies was analyzed, screening for unique medicinal compounds. Searched for depression-related targets. Constructed PPI networks. Performed GO and KEGG analyses. Built a network of differential components, and conducted molecular docking. In addition, the contents of regaloside before and after lily processing were compared Results: Medicinal lilies and edible lilies have 17 main differences, including regaloside B and regaloside E. There are 179 targets for actives, 2690 for antidepressants, and 98 intersected. Core targets (7) led to 238 GO processes and 107 KEGG pathways. The molecular docking results showed that 17 components, including regaloside B, regaloside E, (25R)-3β,17α-Dihydroxy-5α- spirostan-6-one 3-O-α-L- rhamnopyranosyl-(1→2)-β- D-glucopyranoside (Named: Lilium lancifolium saponin), etc. could act on 7 potential targets such as EGFR, HSP90AA1, STAT3, TNF, etc. to exert antidepressant effects. CONCLUSION: This study employed a network pharmacology combined with a molecular docking approach to compare the active constituents of medicinal and edible lilies in antidepressants, and their pharmacological mechanisms, both theoretically and technically. The phytoconstituents were found to act mainly by inhibiting the inflammatory response in depression. Especially Lilium lancifolium saponin may have a close relationship with antidepressants. These results provide some justification for lilies in the treatment of depression.
摘要：
Mild cognitive impairment (MCI) represents a transitional stage between normal aging and dementia, often considered critical for dementia prevention. Despite its significance, no effective clinical treatment for MCI has yet been established. Emerging evidence has demonstrated a strong association between trimethylamine-N-oxide (TMAO), a prominent metabolite derived from the gut microbiota, and MCI, highlighting its potential as a biomarker and therapeutic target. TMAO has been implicated in increasing MCI risk through its influence on factors such as hypertension, cardiovascular disease, depression, diabetes, and stroke. Moreover, it contributes to MCI by promoting oxidative stress, disrupting the blood–brain barrier, impairing synaptic plasticity, inducing inflammation, causing mitochondrial metabolic disturbances, and facilitating abnormal protein aggregation. This review further explores therapeutic strategies targeting TMAO to mitigate MCI progression.
作者机构：
[Huang, Xiangjun] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Gen Surg, Changsha, Peoples R China.;[Deng, Xinyue] Cent South Univ, Xiangya Sch Med, Changsha, Peoples R China.;[Xu, Hongbo; Yuan, Lamei; Yuan, LM; Deng, Hao; Deng, Xiong] Cent South Univ, Xiangya Hosp 3, Ctr Expt Med, Changsha, Peoples R China.
通讯机构：
[Yuan, LM ; Deng, H] C;Cent South Univ, Xiangya Hosp 3, Ctr Expt Med, Changsha, Peoples R China.
摘要：
The peroxisome biogenesis disorders (PBDs) are a group of rare inherited autosomal recessive diseases characterized by motor and cognitive neurological dysfunction, hypotonia, seizures, feeding difficulties, retinopathy, sensorineural hearing loss, hepatic and renal abnormalities, and chondrodysplasia punctata of long bones, and the clinical expression is variable. Exome sequencing and Sanger sequencing were used to identify the genetic defect for PBDs in a two-generation non-consanguineous Han-Chinese pedigree. Compound heterozygous variants, a novel splicing variant c.113-2A>G and a reported substitution c.890T>C (p.Leu297Pro), in the peroxisomal biogenesis factor 10 gene (PEX10) were detected. The splicing variant c.113-2A>G led to a canonical splice acceptor site inactivation, exon 2 skipping, and in-frame deletions (p.Ala39_Gly65del). The three patients had similar phenotypes of milder PBDs, which were further genetically determined as PBD6B. The findings extend the PEX10 variant spectrum and may provide new insights into PBDs causation and diagnosis, with implications for genetic counseling and clinical management.
作者机构：
[Ma, Chengning; Liu, Lumei; Pan, Siyang; Zhou, Xiang] School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, Hunan, 4120208, China
摘要：
The increasing global aging population has led to a rise in diabetic cognitive dysfunction (DCD), a common complication of diabetes that significantly impacts the health of elderly individuals. Neuronal death is a key factor in cognitive impairment, with studies showing interactions between cellular pyroptosis, apoptosis, and necroptosis in the development of neurodegenerative disorders. This has led to the concept of PANoptosis, where these pathways work together to cause cell death. High glucose levels can induce neuronal damage and cognitive dysfunction in rats, leading to various forms of programmed cell death. It is hypothesized that high glucose can trigger neuronal PANoptosis, resulting in cognitive dysfunction. AIM2, an upstream regulator of PANoptosis, is closely associated with the pathogenesis of DCD. In DCD, dysregulated glucose metabolism induces the release of mitochondrial DNA (mtDNA), which acts as a ligand to activate the cell membrane-bound DNA sensor AIM2. Upon activation, AIM2 oligomerizes and recruits a caspase recruit domain (ASC), forming a complex that activates caspase-1. Caspase-1 activation subsequently triggers the production of pro-inflammatory cytokines, induces pyroptosis, and mediates apoptosis, necroptosis, and PANoptosis in neurons through signaling crosstalk. Understanding the pathophysiological mechanism of AIM2-mediated neuronal PANoptosis in DCD development can aid in early diagnosis and identify new therapeutic targets.
The increasing global aging population has led to a rise in diabetic cognitive dysfunction (DCD), a common complication of diabetes that significantly impacts the health of elderly individuals. Neuronal death is a key factor in cognitive impairment, with studies showing interactions between cellular pyroptosis, apoptosis, and necroptosis in the development of neurodegenerative disorders. This has led to the concept of PANoptosis, where these pathways work together to cause cell death. High glucose levels can induce neuronal damage and cognitive dysfunction in rats, leading to various forms of programmed cell death. It is hypothesized that high glucose can trigger neuronal PANoptosis, resulting in cognitive dysfunction. AIM2, an upstream regulator of PANoptosis, is closely associated with the pathogenesis of DCD. In DCD, dysregulated glucose metabolism induces the release of mitochondrial DNA (mtDNA), which acts as a ligand to activate the cell membrane-bound DNA sensor AIM2. Upon activation, AIM2 oligomerizes and recruits a caspase recruit domain (ASC), forming a complex that activates caspase-1. Caspase-1 activation subsequently triggers the production of pro-inflammatory cytokines, induces pyroptosis, and mediates apoptosis, necroptosis, and PANoptosis in neurons through signaling crosstalk. Understanding the pathophysiological mechanism of AIM2-mediated neuronal PANoptosis in DCD development can aid in early diagnosis and identify new therapeutic targets.
摘要：
Lycium barbarum (goji berry) is a globally valued species used as a health food and traditional medicine. It is known for its bioactive properties, including antioxidant, anti-aging, anticancer, and immune-enhancing effects. This review explores key aspects of L. barbarum research, focusing on its molecular biology, cultivation conditions, and quality characteristics. Advances in genetic studies have identified key genes linked to growth, stress resistance, and secondary metabolite biosynthesis. Cultivation practices, including environmental, irrigation and fertilization factors, significantly affect yield and fruit quality. Additionally, factors such as variety, developmental stage, and post-harvest processing influence the phytochemical composition and bioactive potential of goji berries. However, gaps remain in the comprehensive evaluation of all L. barbarum varieties and their quality attributes. Future studies should emphasize optimizing cultivation methods and refining processing techniques to enhance the functional and medicinal value of goji berries.
Lycium barbarum (goji berry) is a globally valued species used as a health food and traditional medicine. It is known for its bioactive properties, including antioxidant, anti-aging, anticancer, and immune-enhancing effects. This review explores key aspects of L. barbarum research, focusing on its molecular biology, cultivation conditions, and quality characteristics. Advances in genetic studies have identified key genes linked to growth, stress resistance, and secondary metabolite biosynthesis. Cultivation practices, including environmental, irrigation and fertilization factors, significantly affect yield and fruit quality. Additionally, factors such as variety, developmental stage, and post-harvest processing influence the phytochemical composition and bioactive potential of goji berries. However, gaps remain in the comprehensive evaluation of all L. barbarum varieties and their quality attributes. Future studies should emphasize optimizing cultivation methods and refining processing techniques to enhance the functional and medicinal value of goji berries.
期刊：
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING,2025年27(9):1491-1514 ISSN：1386-2073
作者机构：
[Li, Qing; Li, Xiao Ling; Hou, Chao Wen; Shi, Yuhe; Zhu, Jue; Tong, Qiao Zhen; Liu, Xiang Dan] Department of Hunan University of Chinese Medicine, School of Pharmacy, Changsha 410208, Hunan Province, P.R.China
摘要：
AIMS AND OBJECTIVE: This study aimed to identify the bioactive compounds and explore the multi-target mechanisms of Salvia miltiorrhiza Bunge (SMB) against coronary heart disease (CHD) using an integrated serum pharmacochemistry and network pharmacology approach. MATERIALS AND METHODS: The chemical constituents of SMB were characterized by UPLC-MS. The absorbed ingredients and metabolites after oral SMB administration were identified in rat serum. Therapeutic targets of SMB against CHD were predicted by intersecting the targets of absorbed compounds from databases and CHD-associated genes. Protein-protein interaction network, pathway analysis, molecular docking, and molecular dynamic simulation were performed. RESULTS: A total of 61 SMB-derived compounds were identified in rat serum. Network analysis revealed 111 candidate targets highly related to CHD pathways. Further topological analysis identified 10 hub targets and 20 key active compounds, constructing an informative compoundtarget- pathway network. PTGS2 and TNF were predicted as primary targets of SMB against CHD based on molecular dynamic simulation. CONCLUSION: This integrated approach identified bioactive compounds and multi-target mechanisms of SMB against CHD. The results provide scientific evidence supporting SMB's clinical efficacy and reveal potential anti-CHD targets.
