期刊论文

Mechanism of miR-20a Targeting STAT3 Inhibiting Keratinocyte Proliferation and Participating in Psoriasis

中文

中国皮肤性病学杂志

1001-7089

2020

34

4

371-375

10.13735/j.cjdv.1001-7089.201904057

国家自然科学基金(81774326) 湖南省自然科学基金课题(2017JJ2207)。

本校为第一机构

目的探讨miR-20a是否可靶向调控STAT3表达影响角质形成细胞增殖参与银屑病发生发展过程。方法QPCR检测银屑病患者正常皮肤和皮损组织中miR- 20a、STAT3的表达情况,统计分析二者间的表达相关性; QPCR检测miR-20a表达变化对STAT3水平的影响; LUC验证miR-20a和STAT3间的靶向关系; miRNA mimics和STAT3过表达载体共转染后,MTT检测HaCaT细胞的增殖活性。结果miR-20a在银屑病患者皮损组织中特异性低表达,STAT3在皮损组织中高表达,且miR-20a与STAT3表达呈负相关性; QPCR检测miR-20a表达变化对STAT3的影响后发现miR-20a可抑制STAT3表达; LUC实验发现miR-20a可靶向结合STAT3; MTT功能实验结果表明STAT3过...

Objective To explore the role and mechanism of miR-20 a in psoriasis lesions and HaCaT cells. Methods QPCR was used to detect the expression of miR-20 a and STAT3 in normal skin and psoriasis lesions, QPCR was used to detect the effect of miR-20 a expression on STAT3 level in HaCaT cells,LUC verified the relationship between miR-20 a and STAT3.After co-transfection of miRNA mimics and STAT3 overexpression vectors,MTT assayed the proliferative of HaCaT cells.Results miR-20 a was lower expressed in the lesions′ and STAT3 was highly expressed.miR...