期刊论文

中文

中医药信息

1002-2406

2018

35

1

38-42

10.19656/j.cnki.1002-2406.180012

国家自然科学基金项目（No.81302953）； 湖南省中医药科研计划项目（No.2014136）； 湖南省教育厅科研项目（No.15C1050）； 中药粉体与创新药物省部共建国家重点实验室培育基地开放基金（No.ZYFT201510）；

本校为第一机构

目的:探讨补阳还五汤对局灶性脑缺血大鼠神经干细胞移植后凋亡相关基因Bag-1的影响.方法:SD大鼠80只,雌雄各半,复制局灶性脑缺血大鼠模型,造模后按神经功能缺损评分进行分级,随机分为5组,每组15只,分别于移植后7 d、14 d、28 d观察神经功能评分,采用补阳还五汤超微饮片与传统饮片予以干预,于相应时间点处死大鼠后采用免疫组化法检测凋亡基因Bag-1.结果:单纯移植组、移植加中药组大鼠神经功能得到明显恢复,Bag-1蛋白表达上升显著,在各时间点(7 d、14 d、28 d)与模型组比较差异有统计学意义(P＜0.05或P＜0.01),模型加移植加补阳还五汤超微饮片组促进神经功能积分的恢复及上调Bag-1蛋白表达作用优于...

Objective: To investigate the effects of Buyang Huanwu decoction on apoptosis gene Bag - 1 after transplantation of neural stem cells in rats with focal cerebral ischemia. Methods: 80 SD rats were made into the models of focal cerebral ischemia, assessed by Neurological Function Defect Scale (NFDS), and the rats were randomly divided into five groups after modeling, with 15 rats in each group. After the transplantation of7 d, 14 d, and 28 d, interventions of Buyang Huanwu decoction were applied with respective ultramicro herbal pieces and tradi...