期刊论文

Experimental Study on Effect of Active Principle Region of Yangxing Tongmai Formula on BMSCs Transplantation in Myocardium with Blood Stasis Syndrome

中文

世界中医药

1673-7202

2015

1

75-79

10.3969/j.issn.1673-7202.2015.01.020

81102595:国家自然科学基金 2012GXNSFAA053113:广西自然科学基金 2013GXNSFAA019126:广西自然科学基金 1347004－26:广西壮族自治区科学研究与技术开发项目 GZZC1223:广西壮族自治区中医药民族医药自筹经费科研项目

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目的：观察养心通脉部位方（ Active Principle Region of Yangxing Tongmai Formula，apr－YTF）对大鼠骨髓间充质干细胞（ Bone Mesemchymal Stem Cells，BMSCs）移植心血瘀阻证心肌后分化为心肌样细胞的作用。方法：采用“冠脉结扎法”结合血流变学改变建立大鼠心血瘀阻证模型。然后将大鼠分为（ apr－YTF）＋BMSCs组、集落刺激因子（ rhG－CSF）＋BMSCs组、NS＋BMSCs组、NS＋DMEM组。原代培养大鼠BMSCs，体外溴氮胞苷（5－bromouracil Deoxyriboside Brdu）标记后注射至大鼠心肌，采用双染法观察心肌组织中心肌结蛋白Desmin－Brdu和肌球蛋白重链（ Myosin Heavy Chain MHC）－Brdu的表达。结...

Objective:To observe the effect of active principle region of site Yangxing Tongmai Formula( apr-YTF) improving the rat bone marrow mesenchymal stem cells ( BMSCs) transplanted in myocardium with blood stasis syndrome differentiated into myo-cardial like cells.Methods:Coronary ligation combining blood rheology changes was used to set up rat blood stasis syndrome mod-els.Then the rats were divided into ( apr-YTF) +BMSCs group,recombinant human granulocyte colony-stimulating factor +BM-SCs group,NS +BMSCs group,and NS +DMEM group.Primary cultured rat BMSCs,in vitro labeled by 5-romouracil deoxyr...