期刊论文

Research on the Medicate Effect of Clerodendron Bungei Flavornoids on Wnt/β-catenin in Inducing the Apoptosis of HepG2

中文

世界中医药

1673-7202

2016

11

6

954-957

10.3969/j.issn.1673-7202.2016.06.004

国家自然科学基金项目（编号:81503452）；

本校为第一机构

目的：探讨臭牡丹总黄酮诱导肝癌细胞凋亡的作用及机制。方法：体外培养 HepG2细胞，流式细胞术检测臭牡丹总黄酮对细胞凋亡的影响；RT-PCR 法检测臭牡丹总黄酮作用于 HepG2细胞48 h 后对β-catenin、Tcf-4、CD44v6 mRNA 表达的影响。结果：流式细胞术结果显示：随着臭牡丹总黄酮剂量的增加，细胞凋亡比例亦相应增加。PT-PCR 结果显示不同剂量臭牡丹总黄酮作用下β-catenin、Tcf-4、CD44v6 mRNA 的表达均降低，与对照组相比具有统计学意义。结论：臭牡丹总黄酮诱导 HepG2凋亡，能下调β-catenin、Tcf-4、CD44v6基因的表达，其作用机制可能与调控 Wnt／β-catenin 信号通路并抑制其关键基因有关。...

Objective:To discuss the apoptotic effect of Clerodendron bungei flavornoids on liver tumor cells and the possible mechanism.Methods:To culture HepG2 cells in virto,and investigate the apototic effect of clerodendron bungei flavornoidson by flow cytometry.The mRNA expression levels of proteins β-catenin,Tcf-4 and CD44v6 of HepG2 tumor cells were detected by RT-PCR after 48h treatment of flavornoids.Results:Flow cytometry indicates that along with the rising dose of Clerodendron bungei fla-vornoids,the apoptosis rate of HepG2 cancer cells begin...