期刊论文

Study on the Proliferation and Apoptosis of Lung Adenocarcinoma Cells Induced by New Anticancer Prescription of Chanshan Hujin

中文

世界中医药

1673-7202

2019

14

6

1388-1392,1396

10.3969/j.issn.1673-7202.2019.06.009

2015112:湖南省中医药局项目 15C1035:湖南省教育厅项目 C2017027:湖南省卫生计生委课题 9982-1001-011:湖南中医药大学课题 :湖南中医药大学校级重点学科《基础医学》资助

本校为第一机构

目的:观察新型抗癌复方蟾山护金方含药血清对肺腺癌A549细胞增殖的影响,并探讨其能否诱导A549细胞凋亡。方法:将30只SD大鼠随机分成3组,分别为含药血清组、消癌平组、阴性血清组,每组10只,各组依次灌胃蟾山护金方煎剂、消癌平片水溶剂和生理盐水,制备含药血清,利用含药血清做A549细胞增殖抑制实验（MTT实验）、细胞凋亡实验和检测"死亡蛋白酶"caspase-3的表达变化。结果:通过MTT实验,发现蟾山护金方含药血清能够抑制A549细胞的增殖,且呈剂量相关性,与阴性血清组比较,差异有统计学意义（P

Objective: To observe the effects of new anticancer prescription of Chanshan Hujin on the proliferation of lung adenocarcinoma A549 cells,and to investigate that whether it could induce the apoptosis of A519. Methods: A total of 30 SD rats were randomly divided into three groups of a serum containing drug group,a Xiao-Ai-Ping group and a normal saline group,with 10 rats in each group.Each group was intragastric administrated successively with the Decoction of Chan-Shan-Hu-Jin Prescription,Xiao-Ai-Ping water solvent and normal saline,to prepare ...