期刊论文

The study of acupuncture regulating differential miRNA signaling pathway and expression of mir-34c-3p in ischemic hippocampus of CIRI rats

中文

中国康复医学杂志

1001-1242

2020

35

11

1290-1295

10.3969/j.issn.1001-1242.2020.11.003

81874508:国家自然科学基金 81303051:国家自然科学基金 2017-287:湖南省大学生研究性学习和创新性实验计划 201961:湖南省中医药局项目

本校为第一机构

目的:观察针刺调控脑缺血再灌注损伤大鼠缺血侧海马组织差异miRNA信号归属通路及mir-34c-3p表达的作用,探讨mir-34c-3p在脑缺血再灌注损伤中的作用机制。方法:通过线栓法制作局灶性脑缺血再灌注模型,将60只健康SD大鼠随机分为空白组、假手术组、模型组、针刺组,每组15只,共4组。造模成功约180min后开始针刺,每12h一次,连续进行6次。干预结束后,用Zea Longa评分观察大鼠神经功能缺损状况,测定脑梗死面积比,采用miRNA微阵列芯片技术筛选出缺血侧海马组织组间差异基因并归纳信号传导通路,RT-PCR法检测缺血侧海马组织mir-34c-3p的表达水平。结果:造模后,与空白组、假手术组比较,模型组神经功能缺失评...

Objective: To observe the effects of acupuncture on the differential miRNA signaling pathways and mir-34c-3p expression in ischemic hippocampus of cerebral ischemia reperfusion injury rats, and to explore the mechanism of mir-34c-3p in cerebral ischemia reperfusion injury. Method: A focal cerebral ischemia reperfusion model was established by suture method, and 60 healthy SD rats were randomly divided into blank group, sham operation group, model group and acupuncture group, with 15 rats in each group, 4 groups in total. After successful modeli...