期刊论文

Effect of chloroform extract from Solanum nigrum L on clear cell renal cell carcinoma

中文

临床泌尿外科杂志

1001-1420

2020

35

6

454-457

10.13201/j.issn.1001-1420.2020.06.008

长沙市科技计划项目(No:2019-157) 长沙市卫生计划项目(No:2017-15) 湖南中医药大学大学生研究性学习与创新性实验项目(No:2018-04)。

本校为第一机构

目的:探究龙葵氯仿提取物对肾透明细胞癌(CCRCC)细胞增殖和凋亡能力的影响,明确其在CCRCC中作用机制。方法:同步设空白组、顺铂组、龙葵组、抑制剂组、顺铂联合抑制剂组、龙葵联合抑制剂组采用CCK-8法检测CCRCC细胞系的增殖情况,运用AV/PI染色联合流式细胞术检测细胞的凋亡情况,Western Blot法定量检测PI3K/AKT信号通路关键元件的蛋白表达和磷酸化水平。结果:与空白组比较,各组对786-O的增殖均有抑制作用,均能促进细胞凋亡,其中以顺铂联合抑制剂组和龙葵联合抑制剂组最为突出。与空白组比较,各组均能调控PI3K/AKT信号通路,其中龙葵组的p-AKT表达下调最为显著。结论:龙葵氯仿提取物对CCRCC有较好...

Objective: To investigate the effect of chloroform extract of nightshade on the proliferation and apoptosis of renal clear cell carcinoma cells, and clarify its mechanism in renal clear cell carcinoma. Method: Control, DDP, SNL, inhibitor, inhibitors and DDP, SNL combined inhibitor group were synchronously set, and CCK-8 method was used to detect renal clear cell cancer cell proliferation situation. AV/PI flow cytometry was used to detect cell apoptosis, and Western blot method was used for quantitative detection of PI3 K/AKT signal pathway of protein expression and phosphorylation level. Resu...