期刊论文

The Study of Skeletal Muscle＇s Calmodulin Expression in the Early Stage of Rat＇s Limb Ischemic Reperfusion Injury

中文

中国中医骨伤科杂志

1005-0205

2010

18

8

6-8

本校为其他机构

目的:探讨大鼠肢体缺血-再灌注损伤骨骼肌钙调蛋白表达的变化.方法:30只SD大鼠随机分为对照组(control组)、缺血-再灌注组(IR组).对照组所有大鼠用橡皮筋松弛环绕右后肢不阻断血流;IR组按张连元所报道的方法复制SD大鼠右后肢缺血-再灌注模型.各组动物于相应时间点在右后肢相应区域,切取约1000mg腓肠肌,冰盐水快速洗去血液,滤纸吸干水份,剪碎制成组织匀浆,用于RyRl和SERCAl的RT-PCR检测.结果:①骨骼肌型RyRlmRNA的RT-PCR检测结果:在肢体缺血-再灌注损伤再灌注8h内,IR组骨骼肌型RyRlmRNA在再灌注0h、2h表达上调,与对照组相比,其差异有统计学意义(P

Objective：To study the changes of skeletal muscle＇s calmodulin expression in the early stage of rat＇s limb ischemic reperfusion injury.Methods：We divided 30 SD rats into 2 groups in random：control group and ischemic reperfusion group （IR group）. There were 6 rats in the control group,and 24 rats in the IR group,which were divided further into 4 sub-groups with 6 rats in each,according to the time after reperfusion：IR-0 group （0 hour after reperfusion）,IR-2 group （2 hours after reperfusion）,IR-4 group （4 hours after reperfusion）,an...