期刊论文

Effects of Simo Decoction on Sec and SP mRNA in Cold-constraint Stressed Mice

中文

中国中医急症

1004-745X

2013

22

11

1819-1820+1869

国家重点基础研究计划（973计划）资助项目（2009CB523002）；

本校为第一机构

目的 观察四磨汤对寒冷束缚应激小鼠促胰液素(Sec)和P物质(SP) mRNA表达的影响,揭示四磨汤调节胃肠功能紊乱的作用机制.方法 动物随机分组,采用寒冷束缚等多种刺激方法,制备胃肠功能紊乱小鼠模型,分别给予蒸馏水(模型组)、得舒特(对照组)、四磨汤(四磨汤组)治疗,用RT-PCR法检测结肠组织Sec、SP mRNA表达.结果 模型组小鼠结肠组织中Sec mRNA的表达下降、SP mRNA表达上升,与正常组比较差异有统计学意义(P＜0.01);四磨汤组Sec mRNA的表达增加、SP mRNA表达下降,与模型组比较差异有统计学意义(P＜0.05).结论 四磨汤治疗功能性胃肠病的作用机制可能与调节Sec及SP mRNA的变化有关.

Objective： To study the effects of Simo decoction on expression of Sec and SP mRNA in cold-con- straint stressed mice,and to explore the mechanism of Simo decoction on FGIDs. Methods： Gastrointestinal dys- function ＇model was established by exerting the factors of cold-constraint stimulation. And then randomly treated with distilled water（model group）, Dicetel （control guoup） and Simo decoction （SD group）. The expressions of SEC and SP in intestine were measured by RT-PCR. Result： The expressions of SEC mRNA of model group was de- cre...