期刊论文

中文

中国中医急症

1004-745X

2014

23

5

804-807

10.3969/j.issn.1004-745X.2014.05.009

12JJ2046:湖南省自然科学基金 2011SK3096:湖南省科技厅科学研究项目 2013KP0111:湖南省科普计划 :湖南中医药大学研究生校级科研项目

本校为第一机构

目的 观察幽门螺杆菌相关性胃炎(HAG)脾胃湿热证小鼠模型TLR4、IL-6的表达,探寻灭幽汤治疗HAG脾胃湿热证的作用机理.方法 将BABL/c小鼠分成空白组、模型组、灭幽汤组、胃三联组.空白组正常饲养;模型组使用复合因素(湿热环境、高糖高脂饲料、幽门螺杆菌感染)造成HAG脾胃湿热证小鼠模型;灭幽汤组和胃三联组分别使用清热祛湿的灭幽汤和胃三联药物对模型鼠进行治疗.采用免疫组化法检测胃黏膜组织TLR4蛋白的表达,实时荧光定量PCR检测胃黏膜TLR4 mRNA的表达,ELISA检测血清白介素(IL)-6.结果 TLR4蛋白及mRNA,IL-6的表达,灭幽汤组、胃三联组均降低,与模型组比较差异显著(P＜0.01);灭幽汤组与胃三联组比较...

Objective： To observe the expression of TLR4 and IL-6 in rat model of helicobacter pylori associated gastritis with spleen-stomach damp-heat syndrome, and to explore mechanism of Mieyou Decoction treating helicobacter pylori associated gastritis with spleen-stomach dampheat syndrome. Methods： BABL/e rats were divided into control group,model group,Mieyou Decoction group,and Weisanlian group. The control group was given normal feeding while the model group was surrounded by composite factors （hot and humid environment,highsugar and high-fat d...