期刊论文

The Effect of Ginsenoside Rg5 on Proliferation,Apoptosis and Related Factors of Apoptosis of BGC-823 Cellin Gastric Cancer

中文

中国中医急症

1004-745X

2018

27

6

955-958

10.3969/j.issn.1004-745X.2018.06.005

湖南省教育厅课题（16C1242）；

本校为第一机构

目的 观察人参皂苷Rq5对胃癌BGC-823细胞增殖、凋亡及凋亡相关因子Bcl-2相关X蛋白(BAX)、B细胞淋巴瘤-2(Bcl-2)表达的影响,探讨人参皂苷Rq5抑制胃癌生长转移的可能作用机制.方法 采用CCK-8法检测人参皂苷Rq5对BGC-823细胞24、48 h的细胞增殖抑制作用,流式细胞术检测高、低浓度人参皂苷Rq5对BGC-823细胞凋亡的影响,Western blot法检测凋亡相关因子Bax、Bcl-2蛋白的表达. 结果与空白组比较, 人参皂苷Rq550、100、200 μmol/L 24 h存活率降低(P＜0.05), 人参皂苷Rq512.5、25、50、100、200 μmol/L 48 h存活率降低(P＜0.05);与空白组比较,低浓度人参皂苷Rq5凋亡率升高(P＞0.05),高浓度人参皂苷Rq5凋...

Objective: To study the effect of qinsenoside Rq5on the proliferation,apoptosis and the expression of Bax and Bcl-2 of BGC-823 cell in qastric cancer,and to investiqate the possible mechanism of qinsenoside Rq5 in inhibitinq the qrowth and metastasis of qastric cancer. Methods: CCK-8 method was used to detect the inhibitory effect of qinsenoside Rq5on the proliferation of BGC-823 cells. Flow cytometry was used to detect the effect of qinsenoside Rq5on BGC-823 cell apoptosis. Western blot was used to detect the expression of Bax and Bcl-2 protei...