期刊论文

Effect of Simiao Yongan Decoction on Anastomotic Restenosis and Expression of VEGF Protein in Rats with Microvascular Anastomosis

中文

中国中医急症

1004-745X

2018

27

4

637-639+643

10.3969/j.issn.1004-745X.2018.04.022

湖南中医药大学青年教师科研基金课题 湖南省研究生创新课题（CX2017B422）

本校为第一机构

目的 观察四妙勇安汤对大鼠尾动脉血管吻合术后吻合口再狭窄及血管内皮生长因子(VEGF)蛋白的作用.方法 雄性SD大鼠随机分为两组,每组12只,均采用尾动脉离断后行显微镜下吻合手术造模,造模后12h进行干预,实验组予四妙勇安汤灌服,对照组予0.9％氯化钠注射液灌服.分别于造模后第3日和第7日的末次灌胃2h后分批留取血管组织标本,HE染色观察、免疫组织化学法检测VEGF蛋白表达,并采用图像分析软件IPP 6.0(Image-Pro-Plus 6.0)进行分析.结果 与对照组比较,四妙勇安汤组在术后第3日和第7日时的血管内膜面积均明显增加(P＜0.05),VEGF蛋白表达升高(P＜0.05),而吻合口血管狭窄程度减低(P＜0.05).结论 四妙勇...

Objective:To study the effect of Simiao Yongan Decoction on anastomotic restenosis and expression of VEGF protein in rats with microvascular anastomosis.Methods:Male SD rats were randomly divided into two groups (n=12).All of them were treated with the anastomosis under the microscope after the tail artery were cutoff.After the model was established,Simiao Yongan Decoction and normal saline was respectively performed.On the 3rd day and the 7th day after the operation,the blood vessel tissue samples were collected at 2 h after the last Feeding.E...