期刊论文

Effect of Jinshui Liujun Decoction and its Decomposed Prescription Serum on High Mucus Secretion Induced by HNE in A549 Cells

中文

中国中医急症

1004-745X

2019

28

5

753-756

10.3969/j.issn.1004-745X.2019.05.001

国家自然科学基金资助项目(81473564) 湖南省研究生科研创新项目(CX2017B423).

本校为第一机构

目的探讨金水六君煎及其拆方对A549细胞黏液高分泌模型黏蛋白MUC5AC、MUC5B表达的影响。方法制备金水六君煎及其拆方、生理盐水大鼠血清,将人肺腺癌细胞A549分为空白对照组、模型组、金水六君煎组、养阴组、化痰组。采用人中性粒细胞弹性蛋白酶（HNE）25 nmol/L诱导A549细胞黏液高分泌,20%空白大鼠血清及含药血清进行干预。CCK8法测定细胞活性,实时荧光定量PCR法（rt-PCR）检测细胞MUC5AC mRNA、MUC5B mRNA表达,Western blotting检测细胞MUC5AC mRNA、MUC5B mRNA表达。结果各组细胞活性无明显差异。与空白组比较,模型组细胞黏蛋白MUC5AC m RNA、MUC5B m RNA与蛋白表达明显增多（P

Objective: To investigate the effects of Jinshui Liujun Decoction and its decomposition on the expression of mucin MUC5AC and MUC5B in A549 cell mucus hypersecretion model. Methods: Rat serum of Jinshui Liujun Decoction,its decomposed prescription and normal saline was prepared. Human lung adenocarcinoma A549 was divided into the blank control group,the model group,Jinshui Liujun group,Yangyin group and Huatan group. Human neutrophil elastase(HNE) 25 nmmol/L was used to induce high mucus secretion in A549 cells and 20% blank rat serum and drug-containing serum were used for intervention. The c...