期刊论文

中文

中国中医急症

1004-745X

2020

29

9

1524-1527,1564

10.3969/j.issn.1004-745X.2020.09.006

国家自然科学基金项目(81503442) 湖南中医药大学研究生创新课题项目(2019CX13)。

本校为第一机构

目的探讨六味地黄汤对慢性肾衰竭大鼠肾脏Bax和Bcl-2表达的影响。方法将45只雄性SD大鼠随机分为空白组（10只）、假手术组（10只）及手术组（25只）,手术组予以5/6肾切除造模,造模成功后随机分为模型组及治疗组。治疗组予六味地黄汤灌胃,其余3组予等体积蒸馏水灌胃。干预8周后检测各组大鼠血肌酐、血尿素氮、尿蛋白定量水平;HE、Masson染色观察大鼠肾脏组织形态学变化;免疫组化及实时荧光定量PCR分别检测大鼠Bax及Bcl-2蛋白及mRNA的表达水平。结果空白组及假手术组大鼠肾脏未观察到明显病理变化,模型组存在不同程度的肾小管及肾间质病变,治疗组较模型组病灶明显减轻;模型组血肌酐、血尿素氮、尿...

Objective:To investigate the effect of Liuwei Dihuang Decoction on the expression of Bax and Bcl-2 in rats with chronic renal failure.Methods:A total of 45 male SD rats were randomly divided into blank group(10 rats),sham operation group(10 rats)and operation group(25 rats).The operation group received 5/6 nephrec⁃tomy and was randomly divided into the model group and the treatment group after successful modeling.The treat⁃ment group was given Liuwei Dihuang Decoction and the other three groups were given equal volume distilled wa⁃ter.Quanti...